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The map label for this gene is tgtA

Identifier: 15668612

GI number: 15668612

Start: 389623

End: 391275

Strand: Reverse

Name: tgtA

Synonym: MJ0436

Alternate gene names: 15668612

Gene position: 391275-389623 (Counterclockwise)

Preceding gene: 15668613

Following gene: 15668611

Centisome position: 23.5

GC content: 32.55

Gene sequence:

>1653_bases
TTGGAAATTATAGAATTCCAAGAAAGAATCGGAGTGGATGTTGGAACAATATTAGACATCCCAACACCACCAGATGTTGA
TAGAGAAAGAGCTGAGAAAGAATTAGAAGAAACTTTAAAGAGAGCTAAAGCATCTATAGAATTAAAGGAAGAGAGAGGAT
TTAAATTATTACTAAATGGAACTGTTCAAGGATCTACTTATTTAGATTTGAGGCAAAAATCTGCCAAAGAGATGGCCAAG
TTAGGATTTGATATCTATCCAATAGGAGCTGTTGTTCCATTGATGGAGCAATACAGATACAGAGATGTTGCTGAAATTAT
AATAAACTCAAAGATGTATCTACCAACAAACAAGCCAGTGCATTTATTTGGTTGTGGGCATCCAATGTTCTTTGCTTTAG
CTGTTGCTTTGGGCTGTGATTTGTTTGATTCTGCTGCTTATGCATTATATGCTAAGGATGACAGATATTTAACTGAAAGA
GGGACTTTACACTTGGAAGAGATTAAAGATTTAAAGGCATTTCCATGTTCATGTCCTGTTTGTTCAAGCTATACACCAAA
AGAATTGGCAAGTTTAAATAAAAAAGAGAGAGAAAGATTGTTAGCTGAACACAACCTATATGTAACTTTTGAAGAGATAA
ATAGAATAAAGCAGGCAATAAGAGATGGTAGTTTATGGGAATTGGTTGAGGAGAGAGTTAGATGTCATCCAAAGCTTTTG
GAAGCTTATAGGGTTGTTAGGAAATACATAGACTATATTGAAAAATTCGACCCAGTAACTAAAAAATCTGCCTTCTTCTA
TACTGGAATTGAATCGATGTTTAGACCAGAGGTTTTGAGACATAAGAAGAGATTGAAGAGGCTTAGATATGAAAAAGTTT
ATATTACAACTGTATCAAGCTCTATAGAAAAGCCATATCATGAGCATTTAAATGTAGTTGAGACAGATGTCGATATCTTA
ATTAAAGACCCAGTCTTTGGGTTTATTCCATACTACATAGATACCGTTTATCCACTATCTCAACATGAAATTCCTGAGCT
TTTTGATTATGAAAAAGAAATAAACAAGAGGTTTGTTGATGAATTTATTGATTGGTTAAAGAAAAAAATCGGAGAAGACA
ATATATTAGATATAATGACCTACAATTATTATATAAATTACTTCTCTGCAAATAAAAAAATTAATGCCGATGCTTTAAGG
ATTAGGAAAATGTTACAGTATCAGTATGGTTTTGATATAATTGACGATGAACTAATGAATAAAATAAAAGTTGTTAGAAG
CAAAACTACTGGTAGATTAAGGCAGGTTTTGGATGAAAATGGAGAAATTTTATTCTCAGTTAGGAGTAATGACAACCTCT
TAATACCTTCTGAAAAAGGAGCCAAATTGTTGTGGAAAAAAATTCCTTTCCCAAAATATAGGGTTGTTGTTAATAAAGAG
GCGGAGGAATTTGCAAGAGAGGGGAGAAATGTATTTGCCAAATTTGTTATTGATTGTGATGAGGAGTTAAGACCTTACGA
AGAAGTTTTGGTTGTTAATGAAGATGATGAACTCTTAGCTTATGGAACAACGATTTTAAATGGTATTGAGTTAAGAGAAT
TTAATTATGGATTGGCTGTTAAAGTAAGAGGAGGATTAAAAATAAATAAGTGA

Upstream 100 bases:

>100_bases
AGAAAAAGGGATTCACAAATTAATTGGCTTTGATAAAGTAATTGTTACAGATAGTGGTTCTTTTCAGTTAGGAGTTTATG
GAGATGTTGAAGTTGAACCA

Downstream 100 bases:

>100_bases
TAATTATGAATATCAACGAAATTAAAAGAAAAATTATCCCAATTCTATTAAAACATGGTGTTAAAAGAGCATCAATATTT
GGTAGTTATGCAAGAAATGA

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MEIIEFQERIGVDVGTILDIPTPPDVDRERAEKELEETLKRAKASIELKEERGFKLLLNGTVQGSTYLDLRQKSAKEMAK
LGFDIYPIGAVVPLMEQYRYRDVAEIIINSKMYLPTNKPVHLFGCGHPMFFALAVALGCDLFDSAAYALYAKDDRYLTER
GTLHLEEIKDLKAFPCSCPVCSSYTPKELASLNKKERERLLAEHNLYVTFEEINRIKQAIRDGSLWELVEERVRCHPKLL
EAYRVVRKYIDYIEKFDPVTKKSAFFYTGIESMFRPEVLRHKKRLKRLRYEKVYITTVSSSIEKPYHEHLNVVETDVDIL
IKDPVFGFIPYYIDTVYPLSQHEIPELFDYEKEINKRFVDEFIDWLKKKIGEDNILDIMTYNYYINYFSANKKINADALR
IRKMLQYQYGFDIIDDELMNKIKVVRSKTTGRLRQVLDENGEILFSVRSNDNLLIPSEKGAKLLWKKIPFPKYRVVVNKE
AEEFAREGRNVFAKFVIDCDEELRPYEEVLVVNEDDELLAYGTTILNGIELREFNYGLAVKVRGGLKINK

Sequences:

>Translated_550_residues
MEIIEFQERIGVDVGTILDIPTPPDVDRERAEKELEETLKRAKASIELKEERGFKLLLNGTVQGSTYLDLRQKSAKEMAK
LGFDIYPIGAVVPLMEQYRYRDVAEIIINSKMYLPTNKPVHLFGCGHPMFFALAVALGCDLFDSAAYALYAKDDRYLTER
GTLHLEEIKDLKAFPCSCPVCSSYTPKELASLNKKERERLLAEHNLYVTFEEINRIKQAIRDGSLWELVEERVRCHPKLL
EAYRVVRKYIDYIEKFDPVTKKSAFFYTGIESMFRPEVLRHKKRLKRLRYEKVYITTVSSSIEKPYHEHLNVVETDVDIL
IKDPVFGFIPYYIDTVYPLSQHEIPELFDYEKEINKRFVDEFIDWLKKKIGEDNILDIMTYNYYINYFSANKKINADALR
IRKMLQYQYGFDIIDDELMNKIKVVRSKTTGRLRQVLDENGEILFSVRSNDNLLIPSEKGAKLLWKKIPFPKYRVVVNKE
AEEFAREGRNVFAKFVIDCDEELRPYEEVLVVNEDDELLAYGTTILNGIELREFNYGLAVKVRGGLKINK
>Mature_550_residues
MEIIEFQERIGVDVGTILDIPTPPDVDRERAEKELEETLKRAKASIELKEERGFKLLLNGTVQGSTYLDLRQKSAKEMAK
LGFDIYPIGAVVPLMEQYRYRDVAEIIINSKMYLPTNKPVHLFGCGHPMFFALAVALGCDLFDSAAYALYAKDDRYLTER
GTLHLEEIKDLKAFPCSCPVCSSYTPKELASLNKKERERLLAEHNLYVTFEEINRIKQAIRDGSLWELVEERVRCHPKLL
EAYRVVRKYIDYIEKFDPVTKKSAFFYTGIESMFRPEVLRHKKRLKRLRYEKVYITTVSSSIEKPYHEHLNVVETDVDIL
IKDPVFGFIPYYIDTVYPLSQHEIPELFDYEKEINKRFVDEFIDWLKKKIGEDNILDIMTYNYYINYFSANKKINADALR
IRKMLQYQYGFDIIDDELMNKIKVVRSKTTGRLRQVLDENGEILFSVRSNDNLLIPSEKGAKLLWKKIPFPKYRVVVNKE
AEEFAREGRNVFAKFVIDCDEELRPYEEVLVVNEDDELLAYGTTILNGIELREFNYGLAVKVRGGLKINK

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs. Can also utilize guanine as substrate

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Escherichia coli, GI1786607, Length=239, Percent_Identity=28.8702928870293, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920510, Length=225, Percent_Identity=25.7777777777778, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATGT_METJA (Q57878)

Other databases:

- EMBL:   L77117
- PIR:   D64354
- RefSeq:   NP_247410.1
- ProteinModelPortal:   Q57878
- GeneID:   1451296
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0436
- NMPDR:   fig|243232.1.peg.447
- TIGR:   MJ0436
- HOGENOM:   HBG512800
- OMA:   ITNSYIT
- ProtClustDB:   PRK13534
- BioCyc:   MJAN243232:MJ_0436-MONOMER
- HAMAP:   MF_01634
- InterPro:   IPR004804
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR002616
- InterPro:   IPR004521
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- SMART:   SM00359
- TIGRFAMs:   TIGR00432
- TIGRFAMs:   TIGR00449
- TIGRFAMs:   TIGR00451

Pfam domain/function: PF01472 PUA; PF01702 TGT; SSF88697 PUA-like; SSF51713 tRNA_ribo_trans

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 64279; Mature: 64279

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 89-89

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIEFQERIGVDVGTILDIPTPPDVDRERAEKELEETLKRAKASIELKEERGFKLLLNG
CCHHHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCCEEEEEC
TVQGSTYLDLRQKSAKEMAKLGFDIYPIGAVVPLMEQYRYRDVAEIIINSKMYLPTNKPV
EECCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCE
HLFGCGHPMFFALAVALGCDLFDSAAYALYAKDDRYLTERGTLHLEEIKDLKAFPCSCPV
EEEECCCHHHHHHHHHHCCHHHCCCEEEEEECCCCEECCCCCEEHHHHHHHHCCCCCCCC
CSSYTPKELASLNKKERERLLAEHNLYVTFEEINRIKQAIRDGSLWELVEERVRCHPKLL
CCCCCHHHHHHCCHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHH
EAYRVVRKYIDYIEKFDPVTKKSAFFYTGIESMFRPEVLRHKKRLKRLRYEKVYITTVSS
HHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHCEEEEEEEHH
SIEKPYHEHLNVVETDVDILIKDPVFGFIPYYIDTVYPLSQHEIPELFDYEKEINKRFVD
HHCCCHHHCCCEEECCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
EFIDWLKKKIGEDNILDIMTYNYYINYFSANKKINADALRIRKMLQYQYGFDIIDDELMN
HHHHHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
KIKVVRSKTTGRLRQVLDENGEILFSVRSNDNLLIPSEKGAKLLWKKIPFPKYRVVVNKE
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHCCCCCEEEEECCC
AEEFAREGRNVFAKFVIDCDEELRPYEEVLVVNEDDELLAYGTTILNGIELREFNYGLAV
HHHHHHCCHHEEHHHHCCCCHHCCCCHHEEEECCCCCEEEEHHHHHCCEEEEEECCCEEE
KVRGGLKINK
EEECCEEECC
>Mature Secondary Structure
MEIIEFQERIGVDVGTILDIPTPPDVDRERAEKELEETLKRAKASIELKEERGFKLLLNG
CCHHHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCCEEEEEC
TVQGSTYLDLRQKSAKEMAKLGFDIYPIGAVVPLMEQYRYRDVAEIIINSKMYLPTNKPV
EECCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCE
HLFGCGHPMFFALAVALGCDLFDSAAYALYAKDDRYLTERGTLHLEEIKDLKAFPCSCPV
EEEECCCHHHHHHHHHHCCHHHCCCEEEEEECCCCEECCCCCEEHHHHHHHHCCCCCCCC
CSSYTPKELASLNKKERERLLAEHNLYVTFEEINRIKQAIRDGSLWELVEERVRCHPKLL
CCCCCHHHHHHCCHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHH
EAYRVVRKYIDYIEKFDPVTKKSAFFYTGIESMFRPEVLRHKKRLKRLRYEKVYITTVSS
HHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHCEEEEEEEHH
SIEKPYHEHLNVVETDVDILIKDPVFGFIPYYIDTVYPLSQHEIPELFDYEKEINKRFVD
HHCCCHHHCCCEEECCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
EFIDWLKKKIGEDNILDIMTYNYYINYFSANKKINADALRIRKMLQYQYGFDIIDDELMN
HHHHHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
KIKVVRSKTTGRLRQVLDENGEILFSVRSNDNLLIPSEKGAKLLWKKIPFPKYRVVVNKE
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHCCCCCEEEEECCC
AEEFAREGRNVFAKFVIDCDEELRPYEEVLVVNEDDELLAYGTTILNGIELREFNYGLAV
HHHHHHCCHHEEHHHHCCCCHHCCCCHHEEEECCCCCEEEEHHHHHCCEEEEEECCCEEE
KVRGGLKINK
EEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.000014 {7-(aminomethyl)-7-deazaguanine}} 0.00006 {guanine}} 0.000053 {guanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087