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The map label for this gene is hisF

Identifier: 15668587

GI number: 15668587

Start: 370907

End: 371725

Strand: Direct

Name: hisF

Synonym: MJ0411

Alternate gene names: 15668587

Gene position: 370907-371725 (Clockwise)

Preceding gene: 15668586

Following gene: 15668591

Centisome position: 22.28

GC content: 35.04

Gene sequence:

>819_bases
ATGCTAACAAAGAGAATTATTCCATGCTTAGATATTAAAGACGGAAGAGTTGTTAAAGGAACTAAGTTTTTGAATTTGAG
GGATGCTGGAGACCCAGTTGAGTTAGCCCAATACTATGATGATGAAGGAGCTGATGAGCTTGTATTTTTAGATATAACCG
CCTCAGCTGAAAAGAGGGACATAATTATTGATGTTGTAGAGAGAACAGCTGAAAAAGTATTTATCCCATTAACTGTTGGT
GGAGGAATTAAGTCAATTGAAGATTTTAGGAGAATACTGAGAGCCGGGGCTGATAAAGTTTCGATAAACACTGCCGCAGT
AAAAAATCCAAATTTAATTAAAGAGGCAAGTGAGATATTTGGCTCTCAATGTGTTGTTGTTGCAATTGATGCTAAAAGAC
ACTATGTTAATGAAGATGAGATAGATAAGATAAATAAAAATGTCGTTAAAGTAGAGGATGGTTATTGCTGGTTTGAAGTT
TATATATACGGAGGGAGAAAAGAAACAGGTATAGATGCCATAAACTGGGCTAAAAAAGTTGAAGAATTGGGAGCTGGAGA
GATTTTATTGACAAGTATTGATAAAGATGGGACAAAAAGTGGCTATGATTTGATATTGACAAAGGAGATTTCTAAAAGTG
TTAAACTCCCTGTTATTGCAAGTGGAGGTTGTGGAAAACCAGAACATGTTTATGAGGCATTTGTTTATGGAAAGGCAGAT
GCCGCATTAATGGCAGGGATATTGCACTATAGAGAATATACAATAGAAGAGATAAAAAAATACTGTGCTGATAGAGGAAT
ACCTATGAGATTATTATAA

Upstream 100 bases:

>100_bases
TTTCAGAACTAACTGAAGTAGAAAAATCTGAAAAGGACTTTGAAGATTATTTAGAATAAGAAATATAATAAAAAGTAAGT
ATAATAAAAATAGGGATAGG

Downstream 100 bases:

>100_bases
ACTTATAAACCTTTTTTATTTTTATTTTAGAGATTTTAAGACCTCATCTTTCAATATGTTTAGTATTTTCTTTCTATATT
CAAGGAATTCTATACTTGTT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MLTKRIIPCLDIKDGRVVKGTKFLNLRDAGDPVELAQYYDDEGADELVFLDITASAEKRDIIIDVVERTAEKVFIPLTVG
GGIKSIEDFRRILRAGADKVSINTAAVKNPNLIKEASEIFGSQCVVVAIDAKRHYVNEDEIDKINKNVVKVEDGYCWFEV
YIYGGRKETGIDAINWAKKVEELGAGEILLTSIDKDGTKSGYDLILTKEISKSVKLPVIASGGCGKPEHVYEAFVYGKAD
AALMAGILHYREYTIEEIKKYCADRGIPMRLL

Sequences:

>Translated_272_residues
MLTKRIIPCLDIKDGRVVKGTKFLNLRDAGDPVELAQYYDDEGADELVFLDITASAEKRDIIIDVVERTAEKVFIPLTVG
GGIKSIEDFRRILRAGADKVSINTAAVKNPNLIKEASEIFGSQCVVVAIDAKRHYVNEDEIDKINKNVVKVEDGYCWFEV
YIYGGRKETGIDAINWAKKVEELGAGEILLTSIDKDGTKSGYDLILTKEISKSVKLPVIASGGCGKPEHVYEAFVYGKAD
AALMAGILHYREYTIEEIKKYCADRGIPMRLL
>Mature_272_residues
MLTKRIIPCLDIKDGRVVKGTKFLNLRDAGDPVELAQYYDDEGADELVFLDITASAEKRDIIIDVVERTAEKVFIPLTVG
GGIKSIEDFRRILRAGADKVSINTAAVKNPNLIKEASEIFGSQCVVVAIDAKRHYVNEDEIDKINKNVVKVEDGYCWFEV
YIYGGRKETGIDAINWAKKVEELGAGEILLTSIDKDGTKSGYDLILTKEISKSVKLPVIASGGCGKPEHVYEAFVYGKAD
AALMAGILHYREYTIEEIKKYCADRGIPMRLL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=277, Percent_Identity=42.2382671480144, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI87082028, Length=224, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=39.8089171974522, Blast_Score=204, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_METJA (Q57854)

Other databases:

- EMBL:   L77117
- PIR:   C64351
- RefSeq:   NP_247385.1
- ProteinModelPortal:   Q57854
- SMR:   Q57854
- GeneID:   1451271
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0411
- NMPDR:   fig|243232.1.peg.422
- TIGR:   MJ0411
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   MJAN243232:MJ_0411-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 30168; Mature: 30168

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDIKDGRVVKGTKFLNLRDAGDPVELAQYYDDEGADELVFLDITASAEKRD
CCCCCCCCEEECCCCEEEECEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCH
IIIDVVERTAEKVFIPLTVGGGIKSIEDFRRILRAGADKVSINTAAVKNPNLIKEASEIF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHC
GSQCVVVAIDAKRHYVNEDEIDKINKNVVKVEDGYCWFEVYIYGGRKETGIDAINWAKKV
CCCEEEEEEECCHHCCCHHHHHHHCCCEEEEECCEEEEEEEEECCCCCCCCHHHHHHHHH
EELGAGEILLTSIDKDGTKSGYDLILTKEISKSVKLPVIASGGCGKPEHVYEAFVYGKAD
HHCCCCCEEEEECCCCCCCCCCEEEEEECHHCCCCCEEEECCCCCCHHHHHHHHEECCCH
AALMAGILHYREYTIEEIKKYCADRGIPMRLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure
MLTKRIIPCLDIKDGRVVKGTKFLNLRDAGDPVELAQYYDDEGADELVFLDITASAEKRD
CCCCCCCCEEECCCCEEEECEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCH
IIIDVVERTAEKVFIPLTVGGGIKSIEDFRRILRAGADKVSINTAAVKNPNLIKEASEIF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHC
GSQCVVVAIDAKRHYVNEDEIDKINKNVVKVEDGYCWFEVYIYGGRKETGIDAINWAKKV
CCCEEEEEEECCHHCCCHHHHHHHCCCEEEEECCEEEEEEEEECCCCCCCCHHHHHHHHH
EELGAGEILLTSIDKDGTKSGYDLILTKEISKSVKLPVIASGGCGKPEHVYEAFVYGKAD
HHCCCCCEEEEECCCCCCCCCCEEEEEECHHCCCCCEEEECCCCCCHHHHHHHHEECCCH
AALMAGILHYREYTIEEIKKYCADRGIPMRLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087