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The map label for this gene is speE

Identifier: 15668488

GI number: 15668488

Start: 292554

End: 293435

Strand: Reverse

Name: speE

Synonym: MJ0313

Alternate gene names: 15668488

Gene position: 293435-292554 (Counterclockwise)

Preceding gene: 15668490

Following gene: 15668487

Centisome position: 17.62

GC content: 32.2

Gene sequence:

>882_bases
GTGAATCAAAATAATGATTTTAAATGCCATATTTGGTTTACAGAGTATCATAACAACAATGTAGCTCTTTCAGTTAGAGT
TAAGGATATCTTATATAGGGAGAAATCAGGATTTCAAGAGATAGAGATTATTGACACCTATGATTTTGGGAAGGCATTAA
TTTTAGATAACACTTTTCAGACAACAGAGAGAGATGAATTTATTTATCATGAATTAATATCCCACATACCTCTTTTCACC
CATCCAAATCCAAGGAATGTTTTGGTTATTGGAGGAGGGGATGGAGGGACTGTTAGGGAAGTTGTTAAGCATAAATCAGT
TGAAACAGTGGATTTTGTAGAGTTGGATGAAAAGGTTATTGAAGCTTGTAAAAAGTATATGCCAAAATTGAGCTGTGAAA
TTGATAATGAGAAGGTAAATTTGATAATAACAGATGGAATTAAGTATGTTGCTGAAACAGAGAAGAAGTATGATGTGATT
ATTGTTGATTGTCCAGACCCTGTTGGGCCTGCTAAGGGGCTTTTTGAGAAAGAATTTTATAAAAATGTGTTTAAATGTTT
AAATGATGATGGAATTATGGTTCAGCAATCAGAGAGTCCATTGTATAACTTAGATTTGATACAAAATATCTGCAGATATT
TAAAAGATGCTGGATTTAAGATAATTATGCCATACACCTACCCAATGCCAACATATCCAAGTGGATTCTGGAGCTTTACA
TTAGCATCTAAAAAATACAACCCATTAGAAGTTGATGAGGCAAGAATAAAAGAAGCTTTAAAAGATATGGAAACTAAATA
CTATGATGAAGAAGTCCATAAGGGAATATTTTTAGCAGCACCTAAATTTTTAAAAGATGCTGTTAAAAAAGCTCTTGAAT
AA

Upstream 100 bases:

>100_bases
AGCACCATGCAAAAATTTTTATAGACAAAAGGTTTAATTAACATTACTCCATTTATAACTATTATATTTACACCTCCTAA
AATAAATAAGGTGATGTAGA

Downstream 100 bases:

>100_bases
TTTTATTTTCGTCTTTTTTATTTATACACTCATTTCGAATTTTTACCATTTTAACCATTTTATTTTTAAATATTTTTAAA
TATGTGTTTGACTATCTATA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNQNNDFKCHIWFTEYHNNNVALSVRVKDILYREKSGFQEIEIIDTYDFGKALILDNTFQTTERDEFIYHELISHIPLFT
HPNPRNVLVIGGGDGGTVREVVKHKSVETVDFVELDEKVIEACKKYMPKLSCEIDNEKVNLIITDGIKYVAETEKKYDVI
IVDCPDPVGPAKGLFEKEFYKNVFKCLNDDGIMVQQSESPLYNLDLIQNICRYLKDAGFKIIMPYTYPMPTYPSGFWSFT
LASKKYNPLEVDEARIKEALKDMETKYYDEEVHKGIFLAAPKFLKDAVKKALE

Sequences:

>Translated_293_residues
MNQNNDFKCHIWFTEYHNNNVALSVRVKDILYREKSGFQEIEIIDTYDFGKALILDNTFQTTERDEFIYHELISHIPLFT
HPNPRNVLVIGGGDGGTVREVVKHKSVETVDFVELDEKVIEACKKYMPKLSCEIDNEKVNLIITDGIKYVAETEKKYDVI
IVDCPDPVGPAKGLFEKEFYKNVFKCLNDDGIMVQQSESPLYNLDLIQNICRYLKDAGFKIIMPYTYPMPTYPSGFWSFT
LASKKYNPLEVDEARIKEALKDMETKYYDEEVHKGIFLAAPKFLKDAVKKALE
>Mature_293_residues
MNQNNDFKCHIWFTEYHNNNVALSVRVKDILYREKSGFQEIEIIDTYDFGKALILDNTFQTTERDEFIYHELISHIPLFT
HPNPRNVLVIGGGDGGTVREVVKHKSVETVDFVELDEKVIEACKKYMPKLSCEIDNEKVNLIITDGIKYVAETEKKYDVI
IVDCPDPVGPAKGLFEKEFYKNVFKCLNDDGIMVQQSESPLYNLDLIQNICRYLKDAGFKIIMPYTYPMPTYPSGFWSFT
LASKKYNPLEVDEARIKEALKDMETKYYDEEVHKGIFLAAPKFLKDAVKKALE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=281, Percent_Identity=38.7900355871886, Blast_Score=213, Evalue=1e-55,
Organism=Escherichia coli, GI1786312, Length=273, Percent_Identity=36.996336996337, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25154106, Length=273, Percent_Identity=39.9267399267399, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI212645616, Length=321, Percent_Identity=34.2679127725857, Blast_Score=183, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6325326, Length=277, Percent_Identity=35.7400722021661, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323175, Length=281, Percent_Identity=37.7224199288256, Blast_Score=186, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24645443, Length=277, Percent_Identity=40.072202166065, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI21355941, Length=214, Percent_Identity=39.2523364485981, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_METJA (Q57761)

Other databases:

- EMBL:   L77117
- PIR:   B64339
- RefSeq:   NP_247286.1
- ProteinModelPortal:   Q57761
- SMR:   Q57761
- GeneID:   1451168
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0313
- NMPDR:   fig|243232.1.peg.323
- TIGR:   MJ0313
- HOGENOM:   HBG635113
- OMA:   VKHPLVE
- ProtClustDB:   PRK00811
- BioCyc:   MJAN243232:MJ_0313-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 33899; Mature: 33899

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 39-39 BINDING 94-94 BINDING 114-114 BINDING 163-163 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNNDFKCHIWFTEYHNNNVALSVRVKDILYREKSGFQEIEIIDTYDFGKALILDNTFQ
CCCCCCEEEEEEEEEEECCCEEEEEEEHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCC
TTERDEFIYHELISHIPLFTHPNPRNVLVIGGGDGGTVREVVKHKSVETVDFVELDEKVI
CCCCHHHHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHH
EACKKYMPKLSCEIDNEKVNLIITDGIKYVAETEKKYDVIIVDCPDPVGPAKGLFEKEFY
HHHHHHCCCEEEEECCCEEEEEEECCCHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHH
KNVFKCLNDDGIMVQQSESPLYNLDLIQNICRYLKDAGFKIIMPYTYPMPTYPSGFWSFT
HHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEE
LASKKYNPLEVDEARIKEALKDMETKYYDEEVHKGIFLAAPKFLKDAVKKALE
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MNQNNDFKCHIWFTEYHNNNVALSVRVKDILYREKSGFQEIEIIDTYDFGKALILDNTFQ
CCCCCCEEEEEEEEEEECCCEEEEEEEHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCC
TTERDEFIYHELISHIPLFTHPNPRNVLVIGGGDGGTVREVVKHKSVETVDFVELDEKVI
CCCCHHHHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHH
EACKKYMPKLSCEIDNEKVNLIITDGIKYVAETEKKYDVIIVDCPDPVGPAKGLFEKEFY
HHHHHHCCCEEEEECCCEEEEEEECCCHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHH
KNVFKCLNDDGIMVQQSESPLYNLDLIQNICRYLKDAGFKIIMPYTYPMPTYPSGFWSFT
HHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEE
LASKKYNPLEVDEARIKEALKDMETKYYDEEVHKGIFLAAPKFLKDAVKKALE
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087