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The map label for this gene is ygfT [C]

Identifier: 15668327

GI number: 15668327

Start: 152592

End: 153047

Strand: Direct

Name: ygfT [C]

Synonym: MJ0155

Alternate gene names: 15668327

Gene position: 152592-153047 (Clockwise)

Preceding gene: 15668326

Following gene: 15668328

Centisome position: 9.16

GC content: 36.18

Gene sequence:

>456_bases
ATGAACCCAAAAATTATCGTCTTAAATCCAGAAAAATGCACCAAATGCTATGACTGTATAAACATCTGTAAAGAGATTCA
TGGAGAGAGTAGAGTAAGGAAGGTTGATGGCATTCCAATATTTTGCATGCAGTGTGAAAACGCTCCCTGTAAAGAGATTT
GTCCTGTTGATGCAATCTATCTAAAAGATGGCATTCCAATTGTAGATAAGGAGAGATGTATTGCTTGTGGAATGTGTGCA
ATTGCCTGCCCAATAGGAGCAATATTTATAAAAAACAGGGTTGCTCATAAATGCACCCTCTGCTTGGATGTTGATAGAAT
AACTCCAGCCTGTGTAGAGGCTTGTAAGGACAAAGCCCTCTTATTAGTATCAGAAGAAACACTTGACATGATGAAAGAAG
AGAAAAGAAAAAAAATACTTAAAATTTTGAGAGAAGAAGCTAAAGAAAACCTATAA

Upstream 100 bases:

>100_bases
TCAATAGACAAATATTATCAGCCAAATACCCTTTATTCATTCCCAAATCTCAGCAAGGAAGAATATTTAGACTATTTGAG
GAAGTTATTGGAGGGGTAGA

Downstream 100 bases:

>100_bases
AAACACTTAAAGGTGAGAAAGCATGTTTGATGATATCCCTGTCTCTGTTGGGCCGATGAATGAGGGGGAGAGAGTTAGGG
GCCCTGATATGTATGTTGAA

Product: formate dehydrogenase, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MNPKIIVLNPEKCTKCYDCINICKEIHGESRVRKVDGIPIFCMQCENAPCKEICPVDAIYLKDGIPIVDKERCIACGMCA
IACPIGAIFIKNRVAHKCTLCLDVDRITPACVEACKDKALLLVSEETLDMMKEEKRKKILKILREEAKENL

Sequences:

>Translated_151_residues
MNPKIIVLNPEKCTKCYDCINICKEIHGESRVRKVDGIPIFCMQCENAPCKEICPVDAIYLKDGIPIVDKERCIACGMCA
IACPIGAIFIKNRVAHKCTLCLDVDRITPACVEACKDKALLLVSEETLDMMKEEKRKKILKILREEAKENL
>Mature_151_residues
MNPKIIVLNPEKCTKCYDCINICKEIHGESRVRKVDGIPIFCMQCENAPCKEICPVDAIYLKDGIPIVDKERCIACGMCA
IACPIGAIFIKNRVAHKCTLCLDVDRITPACVEACKDKALLLVSEETLDMMKEEKRKKILKILREEAKENL

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI87082180, Length=150, Percent_Identity=31.3333333333333, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI87082179, Length=152, Percent_Identity=32.8947368421053, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI2367245, Length=153, Percent_Identity=35.9477124183007, Blast_Score=76, Evalue=8e-16,
Organism=Escherichia coli, GI87082114, Length=183, Percent_Identity=28.4153005464481, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788811, Length=167, Percent_Identity=29.940119760479, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1789079, Length=185, Percent_Identity=28.6486486486486, Blast_Score=73, Evalue=6e-15,
Organism=Escherichia coli, GI1789067, Length=169, Percent_Identity=31.9526627218935, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI226510944, Length=107, Percent_Identity=31.7757009345794, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FER8_METJA (Q57619)

Other databases:

- EMBL:   L77117
- PIR:   D64319
- RefSeq:   NP_247123.1
- ProteinModelPortal:   Q57619
- SMR:   Q57619
- GeneID:   1450999
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0155
- NMPDR:   fig|243232.1.peg.160
- TIGR:   MJ0155
- HOGENOM:   HBG726839
- OMA:   KERCIAC
- ProtClustDB:   CLSK2749846
- BioCyc:   MJAN243232:MJ_0155-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813
- PRINTS:   PR00354

Pfam domain/function: PF00037 Fer4

EC number: 1.2.1.2

Molecular weight: Translated: 16986; Mature: 16986

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
13.9 %Cys+Met (Translated Protein)
10.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
13.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKIIVLNPEKCTKCYDCINICKEIHGESRVRKVDGIPIFCMQCENAPCKEICPVDAIY
CCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHEECCCCEEEEEECCCCCHHHHCCCCEEE
LKDGIPIVDKERCIACGMCAIACPIGAIFIKNRVAHKCTLCLDVDRITPACVEACKDKAL
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCEE
LLVSEETLDMMKEEKRKKILKILREEAKENL
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPKIIVLNPEKCTKCYDCINICKEIHGESRVRKVDGIPIFCMQCENAPCKEICPVDAIY
CCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHEECCCCEEEEEECCCCCHHHHCCCCEEE
LKDGIPIVDKERCIACGMCAIACPIGAIFIKNRVAHKCTLCLDVDRITPACVEACKDKAL
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCEE
LLVSEETLDMMKEEKRKKILKILREEAKENL
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087