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The map label for this gene is cysG [C]

Identifier: 15668312

GI number: 15668312

Start: 138920

End: 139540

Strand: Direct

Name: cysG [C]

Synonym: MJ0140

Alternate gene names: 15668312

Gene position: 138920-139540 (Clockwise)

Preceding gene: 15668310

Following gene: 15668313

Centisome position: 8.34

GC content: 25.44

Gene sequence:

>621_bases
TTGCTTCCTATTTTGTTATCTTTTGAAGGAAAGAAAGTGGCAGTATTTGGTTGTGGAAGTGTAGGAAAAAGGAGAGCTAA
GAAAATATTAAAAAGTGGAGGGATTGTTGATATATACTCTAAAGAATTTGATGAAGAAATAAAAAAATTAAAAGAAAGCA
ATAAAAATCTAAATTTAATTGAAATTGATATTAATCAGTTAAGTGATGAAGAGCTAAAAAATATTATAATGAAGTATGAT
TTTATAGTAACAGCTATCAACGATGAAATTAATAAGAGAATTGTTAAATTAGCTAAAGAGCTAAACAAATTTGTAAATTC
TTCAACAAAGACAGAAGGAGTTAATTTTATCATCCCTGCTTATACAGAGGTTGATGAAGTAATATTCAGCATATACACAA
AAGGAAAAAGTCCTTTAATAGCTAAGCATATAAGAATTTTTGTTGAAAACTATCTAAAATCAACTGACATAAATATGATA
GCCTATATAAGAGAGTTTTTAAAAGAGACAATTCCTAAACAGAAAGATAGGGAAAAAATATTAAAAAAGATTTTTGAAAA
TGAAAAATTTAGAGAAGAATTAAAAAAATTAATAGAGAAGTGGGAAAATGGAAATCATTGA

Upstream 100 bases:

>100_bases
CTTGACAGTCCCTATAATAAGTTTTAAAATTCACTTGTTCTAACTCTAATATAATATTCACTATATCTAATAAATACTTT
CTAACTATGGGAGGGATAAT

Downstream 100 bases:

>100_bases
AATTATAAAGGAGTTTAAAAAGGATATATCAACCATTTTAAAAGATAAATTAGACAGAGTTATTTTATTTGGAAGTTATG
CAAGAGGAGATTATGATGAA

Product: hypothetical protein

Products: sirohydrochlorin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MLPILLSFEGKKVAVFGCGSVGKRRAKKILKSGGIVDIYSKEFDEEIKKLKESNKNLNLIEIDINQLSDEELKNIIMKYD
FIVTAINDEINKRIVKLAKELNKFVNSSTKTEGVNFIIPAYTEVDEVIFSIYTKGKSPLIAKHIRIFVENYLKSTDINMI
AYIREFLKETIPKQKDREKILKKIFENEKFREELKKLIEKWENGNH

Sequences:

>Translated_206_residues
MLPILLSFEGKKVAVFGCGSVGKRRAKKILKSGGIVDIYSKEFDEEIKKLKESNKNLNLIEIDINQLSDEELKNIIMKYD
FIVTAINDEINKRIVKLAKELNKFVNSSTKTEGVNFIIPAYTEVDEVIFSIYTKGKSPLIAKHIRIFVENYLKSTDINMI
AYIREFLKETIPKQKDREKILKKIFENEKFREELKKLIEKWENGNH
>Mature_206_residues
MLPILLSFEGKKVAVFGCGSVGKRRAKKILKSGGIVDIYSKEFDEEIKKLKESNKNLNLIEIDINQLSDEELKNIIMKYD
FIVTAINDEINKRIVKLAKELNKFVNSSTKTEGVNFIIPAYTEVDEVIFSIYTKGKSPLIAKHIRIFVENYLKSTDINMI
AYIREFLKETIPKQKDREKILKKIFENEKFREELKKLIEKWENGNH

Specific function: Multifunctional Enzyme That Catalyze The Sam-Dependent Methylation Of Uroporphyrinogen III At Position C-2 And C-7 To Form Precorrin-2 And Then Position C-12 Or C-18 To Form Trimethylpyrrocorphin 2. It Also Catalyzes The Conversion Of Precorrin-2 Into Sir

COG id: COG1648

COG function: function code H; Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y140_METJA (Q57605)

Other databases:

- EMBL:   L77117
- PIR:   E64317
- RefSeq:   NP_247108.1
- ProteinModelPortal:   Q57605
- GeneID:   1450984
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0140
- NMPDR:   fig|243232.1.peg.145
- TIGR:   MJ0140
- HOGENOM:   HBG541154
- OMA:   GKRRFEK
- ProtClustDB:   CLSK2749844
- BioCyc:   MJAN243232:MJ_0140-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011128
- InterPro:   IPR016040
- InterPro:   IPR006367
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF01210 NAD_Gly3P_dh_N

EC number: 1.3.1.76; 4.99.1.4

Molecular weight: Translated: 23989; Mature: 23989

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPILLSFEGKKVAVFGCGSVGKRRAKKILKSGGIVDIYSKEFDEEIKKLKESNKNLNLI
CCCEEEECCCCEEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCEEE
EIDINQLSDEELKNIIMKYDFIVTAINDEINKRIVKLAKELNKFVNSSTKTEGVNFIIPA
EEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECC
YTEVDEVIFSIYTKGKSPLIAKHIRIFVENYLKSTDINMIAYIREFLKETIPKQKDREKI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
LKKIFENEKFREELKKLIEKWENGNH
HHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLPILLSFEGKKVAVFGCGSVGKRRAKKILKSGGIVDIYSKEFDEEIKKLKESNKNLNLI
CCCEEEECCCCEEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCEEE
EIDINQLSDEELKNIIMKYDFIVTAINDEINKRIVKLAKELNKFVNSSTKTEGVNFIIPA
EEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECC
YTEVDEVIFSIYTKGKSPLIAKHIRIFVENYLKSTDINMIAYIREFLKETIPKQKDREKI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
LKKIFENEKFREELKKLIEKWENGNH
HHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 2; NAD+

Specific reaction: precorrin-2 + NAD+ = sirohydrochlorin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087