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The map label for this gene is purP

Identifier: 15668306

GI number: 15668306

Start: 131467

End: 132552

Strand: Direct

Name: purP

Synonym: MJ0136

Alternate gene names: 15668306

Gene position: 131467-132552 (Clockwise)

Preceding gene: 15668300

Following gene: 15668309

Centisome position: 7.9

GC content: 34.35

Gene sequence:

>1086_bases
ATGATTTCAAAAGATGAGATTTTAGAGATTTTTGATAAATACAACAAAGATGAGATAACAATAGCAACATTAGGAAGCCA
TACCTCTTTGCATATTTTAAAAGGAGCTAAATTAGAAGGCTTTTCTACTGTTTGTATAACTATGAAGGGAAGAGATGTTC
CATACAAGAGATTTAAAGTTGCTGATAAATTTATATATGTTGATAACTTTTCTGACATAAAAAATGAAGAGATTCAAGAG
AAATTGAGAGAGTTAAATTCTATTGTAGTTCCACACGGCTCTTTTATTGCATACTGTGGTTTAGACAATGTGGAAAATAG
TTTTTTAGTCCCAATGTTTGGGAATAGGAGAATATTAAGATGGGAATCAGAGAGAAGCTTAGAAGGAAAGCTGTTGAGAG
AAGCTGGATTAAGAGTTCCTAAGAAGTATGAAAGCCCAGAAGACATTGATGGAACAGTTATAGTCAAATTCCCAGGGGCA
AGAGGTGGAAGAGGTTACTTTATAGCTTCATCAACAGAGGAATTTTATAAAAAAGCTGAGGATTTAAAGAAAAGAGGAAT
ATTGACTGATGAAGACATAGCCAACGCACATATAGAAGAGTATGTTGTTGGAACTAACTTCTGCATACATTATTTCTACT
CTCCATTGAAGGATGAAGTTGAGTTATTGGGAATGGACAAGAGATATGAAAGTAATATAGATGGTTTAGTTAGAATTCCA
GCAAAAGACCAATTAGAGATGAATATAAATCCAAGCTATGTGATTACTGGAAATATTCCAGTTGTTATTAGGGAGAGTTT
ATTACCTCAAGTCTTTGAGATGGGGGATAAGTTAGTTGCTAAGGCTAAAGAGCTTGTTCCACCAGGAATGATTGGACCTT
TCTGTTTGCAGAGTTTATGTAATGAGAATCTTGAATTAGTTGTCTTTGAAATGAGTGCAAGAGTTGATGGTGGAACAAAC
AGCTTTATGAATGGTGGCCCTTACTCTTTCCTATACAATGGAGAGCCATTGAGTATGGGGCAGAGAATTGCAAGAGAGAT
AAAGATGGCTCTACAATTAGATATGATTGATAAGATTATTTCATAA

Upstream 100 bases:

>100_bases
AAAATTCATAATGAAAATTTGAACGCCTTCCTTTGGAAGGCGTTCATTAATGCCTTATCCATCTTAAATATTTGCAAAAA
ACTATAATTAGGTGAAATAA

Downstream 100 bases:

>100_bases
GTTAGTATCTTGTGAAAAATTTAATATTGCTTAGCTTTATTTCTTTAAAACTTTATTCGTTTATTTAGAGGTTTTAAATT
TAATTTCTAAGGGTTTGCTG

Product: 5-formaminoimidazole-4-carboxamide-1-(beta)-D- ribofuranosyl 5'-monophosphate synthetase

Products: NA

Alternate protein names: 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MISKDEILEIFDKYNKDEITIATLGSHTSLHILKGAKLEGFSTVCITMKGRDVPYKRFKVADKFIYVDNFSDIKNEEIQE
KLRELNSIVVPHGSFIAYCGLDNVENSFLVPMFGNRRILRWESERSLEGKLLREAGLRVPKKYESPEDIDGTVIVKFPGA
RGGRGYFIASSTEEFYKKAEDLKKRGILTDEDIANAHIEEYVVGTNFCIHYFYSPLKDEVELLGMDKRYESNIDGLVRIP
AKDQLEMNINPSYVITGNIPVVIRESLLPQVFEMGDKLVAKAKELVPPGMIGPFCLQSLCNENLELVVFEMSARVDGGTN
SFMNGGPYSFLYNGEPLSMGQRIAREIKMALQLDMIDKIIS

Sequences:

>Translated_361_residues
MISKDEILEIFDKYNKDEITIATLGSHTSLHILKGAKLEGFSTVCITMKGRDVPYKRFKVADKFIYVDNFSDIKNEEIQE
KLRELNSIVVPHGSFIAYCGLDNVENSFLVPMFGNRRILRWESERSLEGKLLREAGLRVPKKYESPEDIDGTVIVKFPGA
RGGRGYFIASSTEEFYKKAEDLKKRGILTDEDIANAHIEEYVVGTNFCIHYFYSPLKDEVELLGMDKRYESNIDGLVRIP
AKDQLEMNINPSYVITGNIPVVIRESLLPQVFEMGDKLVAKAKELVPPGMIGPFCLQSLCNENLELVVFEMSARVDGGTN
SFMNGGPYSFLYNGEPLSMGQRIAREIKMALQLDMIDKIIS
>Mature_361_residues
MISKDEILEIFDKYNKDEITIATLGSHTSLHILKGAKLEGFSTVCITMKGRDVPYKRFKVADKFIYVDNFSDIKNEEIQE
KLRELNSIVVPHGSFIAYCGLDNVENSFLVPMFGNRRILRWESERSLEGKLLREAGLRVPKKYESPEDIDGTVIVKFPGA
RGGRGYFIASSTEEFYKKAEDLKKRGILTDEDIANAHIEEYVVGTNFCIHYFYSPLKDEVELLGMDKRYESNIDGLVRIP
AKDQLEMNINPSYVITGNIPVVIRESLLPQVFEMGDKLVAKAKELVPPGMIGPFCLQSLCNENLELVVFEMSARVDGGTN
SFMNGGPYSFLYNGEPLSMGQRIAREIKMALQLDMIDKIIS

Specific function: Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'- monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1- beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates

COG id: COG1759

COG function: function code R; ATP-utilizing enzymes of ATP-grasp superfamily (probably carboligases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURP_METJA (Q57600)

Other databases:

- EMBL:   L77117
- PIR:   H64316
- RefSeq:   NP_247102.1
- PDB:   2R7K
- PDB:   2R7L
- PDB:   2R7M
- PDB:   2R7N
- PDBsum:   2R7K
- PDBsum:   2R7L
- PDBsum:   2R7M
- PDBsum:   2R7N
- ProteinModelPortal:   Q57600
- SMR:   Q57600
- GeneID:   1450977
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0136
- NMPDR:   fig|243232.1.peg.139
- TIGR:   MJ0136
- HOGENOM:   HBG392202
- OMA:   MGQRIAR
- ProtClustDB:   PRK13278
- BioCyc:   MetaCyc:MONOMER-14616
- BioCyc:   MJAN243232:MJ_0136-MONOMER
- HAMAP:   MF_01163
- InterPro:   IPR011761
- InterPro:   IPR013816
- InterPro:   IPR009720
- InterPro:   IPR010672
- InterPro:   IPR013817
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF06849 DUF1246; PF06973 DUF1297

EC number: NA

Molecular weight: Translated: 40825; Mature: 40825

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 27-27 BINDING 94-94 BINDING 230-230 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKDEILEIFDKYNKDEITIATLGSHTSLHILKGAKLEGFSTVCITMKGRDVPYKRFKV
CCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCCHHHHEE
ADKFIYVDNFSDIKNEEIQEKLRELNSIVVPHGSFIAYCGLDNVENSFLVPMFGNRRILR
EEEEEEEECCCCCCHHHHHHHHHHHHCEECCCCCEEEEECCCCCCCCEEEEECCCCEEEE
WESERSLEGKLLREAGLRVPKKYESPEDIDGTVIVKFPGARGGRGYFIASSTEEFYKKAE
ECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHH
DLKKRGILTDEDIANAHIEEYVVGTNFCIHYFYSPLKDEVELLGMDKRYESNIDGLVRIP
HHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCCCCHHHCCCCCEEECC
AKDQLEMNINPSYVITGNIPVVIRESLLPQVFEMGDKLVAKAKELVPPGMIGPFCLQSLC
CCCCEEECCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
NENLELVVFEMSARVDGGTNSFMNGGPYSFLYNGEPLSMGQRIAREIKMALQLDMIDKII
CCCCEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
S
C
>Mature Secondary Structure
MISKDEILEIFDKYNKDEITIATLGSHTSLHILKGAKLEGFSTVCITMKGRDVPYKRFKV
CCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCCHHHHEE
ADKFIYVDNFSDIKNEEIQEKLRELNSIVVPHGSFIAYCGLDNVENSFLVPMFGNRRILR
EEEEEEEECCCCCCHHHHHHHHHHHHCEECCCCCEEEEECCCCCCCCEEEEECCCCEEEE
WESERSLEGKLLREAGLRVPKKYESPEDIDGTVIVKFPGARGGRGYFIASSTEEFYKKAE
ECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHH
DLKKRGILTDEDIANAHIEEYVVGTNFCIHYFYSPLKDEVELLGMDKRYESNIDGLVRIP
HHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCCCCHHHCCCCCEEECC
AKDQLEMNINPSYVITGNIPVVIRESLLPQVFEMGDKLVAKAKELVPPGMIGPFCLQSLC
CCCCEEECCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
NENLELVVFEMSARVDGGTNSFMNGGPYSFLYNGEPLSMGQRIAREIKMALQLDMIDKII
CCCCEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087