| Definition | Methanocaldococcus jannaschii DSM 2661 chromosome, complete genome. |
|---|---|
| Accession | NC_000909 |
| Length | 1,664,970 |
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The map label for this gene is frdB [C]
Identifier: 15668263
GI number: 15668263
Start: 86613
End: 88082
Strand: Direct
Name: frdB [C]
Synonym: MJ0092
Alternate gene names: 15668263
Gene position: 86613-88082 (Clockwise)
Preceding gene: 15668262
Following gene: 15668274
Centisome position: 5.2
GC content: 34.97
Gene sequence:
>1470_bases ATGATAAAGATAACAGTTAAGAGATTTAACGGAGAGAAAGAGTATCTTGAAAGCTATGAAGTCCCAGAAAATATAACAGT TTTAGAGGCCTTAGAGTATATAAATAAGCATTATGAGGCTAATATTTTATTTAGAGCGTCTTGCAGAAATGCTCAGTGTG GAAGTTGTGCTGTAACAATAAATGGAGAGCCAAGATTGGCATGTGAGACAAAGGTAGAGGATGGCATGATAATTGAGCCA TTAAGAGGATTTAAGGTTATTAGAGATTTAATTGTTGATAGGGAACCATATTATAAAAAGTTATTAGGCATAAAAAACTA CCTTATAAGAAAAAATTATCCTGAAGAGCTTGAAATTCTCATTCCCAAATATGTTGAAGAGAACAAAGAGCTTAGAGGTT GTATTGACTGCCTATCTTGCCTCTCTGTATGTCCAGCAAGGGAAGTTAGCGACTATCCTGGCCCAACTTTTATGAGACAG TTGGCGAGATTTGCGTTTGATAAGAGGGACGAAGATGGCAGAGAGATAACTGCATACTTTGAAAATATCTACAACTGCAC AACGTGTGCTAAGTGTGTTGAGGTCTGCCCAAAGGAGATTGATATTGTTCATAGAGCTATAGAAAAGCTTAGAGCTTTAG CATTTAGTAAGGGCTATTATATAGAAAATCACTTAAAAGTTAGGGAAAATGTTTTAAAATATAACAGAAGTGTTGTTGAA GAGGAATTGCCATTATTAAAGCAAGTTGCTGATTTCTATCCAGCTGAAAGTGAGAAATTAAGAGTAGCTTTCTTTACAGG ATGTTTAGTTGATTTTAGACTGCAAAATGTAGGAAAAGATGCAATAAAGGTTTTAAATGCTCATGGTGTGTCAGTTGTTA TTCCTAAAAATCAGGTTTGCTGTGGCTCACCATTCTTCAGAACTGGGCAGAGGGATGTTGCTGAAATGCTAAAAAGAAAA AACTTGGAAATATTTAACAAGTTGGATGTTGATTGTGTTGTAACAATCTGTGCTGGTTGTGGAAGCACATTAAAAAACGA TTATAAGGAGAGAAAGTTTGAAGTTAAAGATATAACAGAGGTTTTAACTGAAGTTGGACTTTTAAAGTATAAACCGTTAA AGATGAGAATCACTTACCACGACCCATGTCATTTGAGGAGAGGACAAAAAATATATAAACAGCCAAGAGAGATTTTAAAG TCCATTCCAGAACTTGAATTTATTGATATAGAGGCAAGGTGTTGTGGAGCTGGAGGAGGAGTTAGGAGTGGAAAACCAGA TATTGCTAACTTAATAGGTAAAAGTAGGGCAAGGATGATTTATGATGCCAATGTAGATGCTGTAATTACAGTTTGCCCAT TCTGTGAATACCACATAAGAGATAGCTTAAAAAGGTTTAAGGAGGAGAATAAAATAGATAAGGAAATAGATGTTATGAAT ATTGTCTCTCTATTGGCTAAAGTCATTTAA
Upstream 100 bases:
>100_bases TACTCTATTTATTTGCAAAATACTCAAAGATTGGTAGATGGCAAGGAATTTTATTTTTAGCTCTATATATAATTGCAATA GCTTCTTTGAGGATGGGATA
Downstream 100 bases:
>100_bases TTTTATTTATATTCCAACGTATATTTTTTTGTCTCCAATTTCAATAACCTCTTTATCTCCTTCTGGCATCTCTTCAACAA TCTCTATATAATCCTTATTG
Product: succinate dehydrogenase/fumarate reductase iron-sulfur subunit
Products: fumarate; reduced acceptor
Alternate protein names: NA
Number of amino acids: Translated: 489; Mature: 489
Protein sequence:
>489_residues MIKITVKRFNGEKEYLESYEVPENITVLEALEYINKHYEANILFRASCRNAQCGSCAVTINGEPRLACETKVEDGMIIEP LRGFKVIRDLIVDREPYYKKLLGIKNYLIRKNYPEELEILIPKYVEENKELRGCIDCLSCLSVCPAREVSDYPGPTFMRQ LARFAFDKRDEDGREITAYFENIYNCTTCAKCVEVCPKEIDIVHRAIEKLRALAFSKGYYIENHLKVRENVLKYNRSVVE EELPLLKQVADFYPAESEKLRVAFFTGCLVDFRLQNVGKDAIKVLNAHGVSVVIPKNQVCCGSPFFRTGQRDVAEMLKRK NLEIFNKLDVDCVVTICAGCGSTLKNDYKERKFEVKDITEVLTEVGLLKYKPLKMRITYHDPCHLRRGQKIYKQPREILK SIPELEFIDIEARCCGAGGGVRSGKPDIANLIGKSRARMIYDANVDAVITVCPFCEYHIRDSLKRFKEENKIDKEIDVMN IVSLLAKVI
Sequences:
>Translated_489_residues MIKITVKRFNGEKEYLESYEVPENITVLEALEYINKHYEANILFRASCRNAQCGSCAVTINGEPRLACETKVEDGMIIEP LRGFKVIRDLIVDREPYYKKLLGIKNYLIRKNYPEELEILIPKYVEENKELRGCIDCLSCLSVCPAREVSDYPGPTFMRQ LARFAFDKRDEDGREITAYFENIYNCTTCAKCVEVCPKEIDIVHRAIEKLRALAFSKGYYIENHLKVRENVLKYNRSVVE EELPLLKQVADFYPAESEKLRVAFFTGCLVDFRLQNVGKDAIKVLNAHGVSVVIPKNQVCCGSPFFRTGQRDVAEMLKRK NLEIFNKLDVDCVVTICAGCGSTLKNDYKERKFEVKDITEVLTEVGLLKYKPLKMRITYHDPCHLRRGQKIYKQPREILK SIPELEFIDIEARCCGAGGGVRSGKPDIANLIGKSRARMIYDANVDAVITVCPFCEYHIRDSLKRFKEENKIDKEIDVMN IVSLLAKVI >Mature_489_residues MIKITVKRFNGEKEYLESYEVPENITVLEALEYINKHYEANILFRASCRNAQCGSCAVTINGEPRLACETKVEDGMIIEP LRGFKVIRDLIVDREPYYKKLLGIKNYLIRKNYPEELEILIPKYVEENKELRGCIDCLSCLSVCPAREVSDYPGPTFMRQ LARFAFDKRDEDGREITAYFENIYNCTTCAKCVEVCPKEIDIVHRAIEKLRALAFSKGYYIENHLKVRENVLKYNRSVVE EELPLLKQVADFYPAESEKLRVAFFTGCLVDFRLQNVGKDAIKVLNAHGVSVVIPKNQVCCGSPFFRTGQRDVAEMLKRK NLEIFNKLDVDCVVTICAGCGSTLKNDYKERKFEVKDITEVLTEVGLLKYKPLKMRITYHDPCHLRRGQKIYKQPREILK SIPELEFIDIEARCCGAGGGVRSGKPDIANLIGKSRARMIYDANVDAVITVCPFCEYHIRDSLKRFKEENKIDKEIDVMN IVSLLAKVI
Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]
COG id: COG0247
COG function: function code C; Fe-S oxidoreductase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 2 4Fe-4S ferredoxin-type domains
Homologues:
Organism=Homo sapiens, GI115387094, Length=218, Percent_Identity=32.1100917431193, Blast_Score=106, Evalue=6e-23, Organism=Escherichia coli, GI1790596, Length=214, Percent_Identity=34.1121495327103, Blast_Score=120, Evalue=3e-28, Organism=Escherichia coli, GI1788576, Length=366, Percent_Identity=27.5956284153005, Blast_Score=108, Evalue=9e-25, Organism=Escherichia coli, GI1786943, Length=237, Percent_Identity=28.6919831223629, Blast_Score=92, Evalue=6e-20, Organism=Escherichia coli, GI48994913, Length=401, Percent_Identity=22.9426433915212, Blast_Score=81, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17533915, Length=227, Percent_Identity=28.6343612334802, Blast_Score=91, Evalue=1e-18, Organism=Saccharomyces cerevisiae, GI6322987, Length=231, Percent_Identity=32.4675324675325, Blast_Score=109, Evalue=8e-25, Organism=Drosophila melanogaster, GI17137106, Length=232, Percent_Identity=32.7586206896552, Blast_Score=106, Evalue=4e-23, Organism=Drosophila melanogaster, GI24643156, Length=219, Percent_Identity=31.0502283105023, Blast_Score=97, Evalue=3e-20,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): Y092_METJA (Q57557)
Other databases:
- EMBL: L77117 - PIR: D64311 - RefSeq: NP_247056.1 - ProteinModelPortal: Q57557 - SMR: Q57557 - GeneID: 1450931 - GenomeReviews: L77117_GR - KEGG: mja:MJ_0092 - NMPDR: fig|243232.1.peg.93 - TIGR: MJ0092 - HOGENOM: HBG539334 - OMA: CAGCYTT - ProtClustDB: PRK06259 - BioCyc: MJAN243232:MJ_0092-MONOMER - InterPro: IPR006058 - InterPro: IPR017896 - InterPro: IPR017900 - InterPro: IPR012675 - InterPro: IPR004017 - InterPro: IPR001041 - InterPro: IPR012285 - InterPro: IPR009051 - InterPro: IPR004489 - Gene3D: G3DSA:3.10.20.30 - Gene3D: G3DSA:1.10.1060.10 - TIGRFAMs: TIGR00384
Pfam domain/function: PF02754 CCG; PF00111 Fer2; SSF54292 Ferredoxin; SSF46548 Helical_ferredxn
EC number: 1.3.99.1
Molecular weight: Translated: 56129; Mature: 56129
Theoretical pI: Translated: 8.02; Mature: 8.02
Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2; PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
4.7 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 6.1 %Cys+Met (Translated Protein) 4.7 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 6.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIKITVKRFNGEKEYLESYEVPENITVLEALEYINKHYEANILFRASCRNAQCGSCAVTI CEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEE NGEPRLACETKVEDGMIIEPLRGFKVIRDLIVDREPYYKKLLGIKNYLIRKNYPEELEIL CCCCCEEEECCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH IPKYVEENKELRGCIDCLSCLSVCPAREVSDYPGPTFMRQLARFAFDKRDEDGREITAYF HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHH ENIYNCTTCAKCVEVCPKEIDIVHRAIEKLRALAFSKGYYIENHLKVRENVLKYNRSVVE HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH EELPLLKQVADFYPAESEKLRVAFFTGCLVDFRLQNVGKDAIKVLNAHGVSVVIPKNQVC HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCC CGSPFFRTGQRDVAEMLKRKNLEIFNKLDVDCVVTICAGCGSTLKNDYKERKFEVKDITE CCCCHHCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH VLTEVGLLKYKPLKMRITYHDPCHLRRGQKIYKQPREILKSIPELEFIDIEARCCGAGGG HHHHHCCCEECCEEEEEEECCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCC VRSGKPDIANLIGKSRARMIYDANVDAVITVCPFCEYHIRDSLKRFKEENKIDKEIDVMN CCCCCCHHHHHHCCHHCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHH IVSLLAKVI HHHHHHHHC >Mature Secondary Structure MIKITVKRFNGEKEYLESYEVPENITVLEALEYINKHYEANILFRASCRNAQCGSCAVTI CEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEE NGEPRLACETKVEDGMIIEPLRGFKVIRDLIVDREPYYKKLLGIKNYLIRKNYPEELEIL CCCCCEEEECCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH IPKYVEENKELRGCIDCLSCLSVCPAREVSDYPGPTFMRQLARFAFDKRDEDGREITAYF HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHH ENIYNCTTCAKCVEVCPKEIDIVHRAIEKLRALAFSKGYYIENHLKVRENVLKYNRSVVE HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH EELPLLKQVADFYPAESEKLRVAFFTGCLVDFRLQNVGKDAIKVLNAHGVSVVIPKNQVC HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCC CGSPFFRTGQRDVAEMLKRKNLEIFNKLDVDCVVTICAGCGSTLKNDYKERKFEVKDITE CCCCHHCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH VLTEVGLLKYKPLKMRITYHDPCHLRRGQKIYKQPREILKSIPELEFIDIEARCCGAGGG HHHHHCCCEECCEEEEEEECCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCC VRSGKPDIANLIGKSRARMIYDANVDAVITVCPFCEYHIRDSLKRFKEENKIDKEIDVMN CCCCCCHHHHHHCCHHCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHH IVSLLAKVI HHHHHHHHC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]
Metal ions: Anions; Diphosphate; Fe [C]
Kcat value (1/min): 10000-13000 [C]
Specific activity: 509
Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]
Substrates: succinate; acceptor
Specific reaction: succinate + acceptor = fumarate + reduced acceptor
General reaction: Redox reaction [C]
Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8688087