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The map label for this gene is gatD

Identifier: 15668191

GI number: 15668191

Start: 21148

End: 22401

Strand: Reverse

Name: gatD

Synonym: MJ0020

Alternate gene names: 15668191

Gene position: 22401-21148 (Counterclockwise)

Preceding gene: 15668195

Following gene: 15668190

Centisome position: 1.35

GC content: 33.49

Gene sequence:

>1254_bases
ATGAATGTTGGAGATGTTATAAGGGTAGAGACAGACAAAGGGATTTTTGAAGGTATCTTATTGCCATCAACTAACGAAAA
TATCATTACAATAAAGATGAAAAACGGTTATAACGTTGGAATATTAAAAGAAAATGTAAAAAATATTGAGATTATTGCTA
AAGGAGAAAAGCCAAAGTATGAACTACCTCCATTAAACATTGAAAAAAATGAAAAATTAAAAACAATCTCTATTTTATCC
ACTGGAGGGACAGTAGCTTCAAAGGTTGATTATAAAACAGGAGCTGTTCATCCTTCTTTTACAGCAGATGATTTAATTAG
GGCTGTACCAGAGCTTTTAGACATTGCCAACATAAAAGGAAGGGCTGTAATGAACATATTAAGCGAAAATATGAAACCAG
AGTATTGGAGAAAGATTGCTGAAGAGATAAAAAAAGAGATAGAAGAGGGAGCTGATGGAATTGTTATTGCCCATGGAACA
GACACTATGAGCTATACAGCTTCAGCTCTCTCATTTATGGTTAAAGCTGATGTCCCAATAATTTTGGTTGGAGCTCAGAG
AAGTAGTGACAGACCTTCATCAGATGCTGCTCTAAACTTAATAAGTGCTGTTTTAGCTGCAAGAGAACCAATTAAAGGAG
TTTATGTAGTAATGCATGGGGAGAGTGGAGATACATTTTGCTATCTACATAAGGGAGTTAAAGTTAGGAAATGCCATTCA
TCAAGAAGAGATGCATTTAAATCTATAAATTCAATTCCAGTAGCTAAGATAAACCCATTTACAAAGGAAATCATCTATTT
GCAGGAAGTTGAAAAATCAGATAACAGCAAAAAGGTAGAGATAAACACAAACTTAGAAGAAAAAGTGGCTTTAATAAAAG
TATATCCTGGAATGGATGGGGAAATTATTAGATTCTATGTTGATAAAGAGTATAAAGGGATTGTCTTAGAAGGGACGGGT
TTAGGTCATGCCCCAGAGTATATATTTGAACACATAAAGTATGCAACTGATAAAGGAGTAGTTGTTGTAATGACTACTCA
AACAATCAATGGAAGAGTAAATATGAACGTCTATTCAAATGGAAGAGAATTACAAAAATTAGGAGTTATTGGTTGTGAAG
ATATGCCTCCAGAAGTTGCATTGGTTAAATTAATGTATCTCTTAGGAAATTATGAGCCAGAGGAAGTTAAAAAATTAATT
AATAAGAATTTGGTTGGGGAGATTGAATATAGGAGCAGATTTGATGCATACTAA

Upstream 100 bases:

>100_bases
TATATATTTAAATATAAAAATTTATATGATAGCTATCTTTTTATAATTTTTATACCCTAATCTATAACAATCTCTAAGAA
TCAGTTTTTAAGGTGGAACG

Downstream 100 bases:

>100_bases
TAATAACAAATTAATTTGGTGATAACATGGAGATTAACTATGAAAAAGTTGGTTTAAAGGTTGGGTTAGAAATTCATCAA
CAGTTAAATACAAAGAGAAA

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNVGDVIRVETDKGIFEGILLPSTNENIITIKMKNGYNVGILKENVKNIEIIAKGEKPKYELPPLNIEKNEKLKTISILS
TGGTVASKVDYKTGAVHPSFTADDLIRAVPELLDIANIKGRAVMNILSENMKPEYWRKIAEEIKKEIEEGADGIVIAHGT
DTMSYTASALSFMVKADVPIILVGAQRSSDRPSSDAALNLISAVLAAREPIKGVYVVMHGESGDTFCYLHKGVKVRKCHS
SRRDAFKSINSIPVAKINPFTKEIIYLQEVEKSDNSKKVEINTNLEEKVALIKVYPGMDGEIIRFYVDKEYKGIVLEGTG
LGHAPEYIFEHIKYATDKGVVVVMTTQTINGRVNMNVYSNGRELQKLGVIGCEDMPPEVALVKLMYLLGNYEPEEVKKLI
NKNLVGEIEYRSRFDAY

Sequences:

>Translated_417_residues
MNVGDVIRVETDKGIFEGILLPSTNENIITIKMKNGYNVGILKENVKNIEIIAKGEKPKYELPPLNIEKNEKLKTISILS
TGGTVASKVDYKTGAVHPSFTADDLIRAVPELLDIANIKGRAVMNILSENMKPEYWRKIAEEIKKEIEEGADGIVIAHGT
DTMSYTASALSFMVKADVPIILVGAQRSSDRPSSDAALNLISAVLAAREPIKGVYVVMHGESGDTFCYLHKGVKVRKCHS
SRRDAFKSINSIPVAKINPFTKEIIYLQEVEKSDNSKKVEINTNLEEKVALIKVYPGMDGEIIRFYVDKEYKGIVLEGTG
LGHAPEYIFEHIKYATDKGVVVVMTTQTINGRVNMNVYSNGRELQKLGVIGCEDMPPEVALVKLMYLLGNYEPEEVKKLI
NKNLVGEIEYRSRFDAY
>Mature_417_residues
MNVGDVIRVETDKGIFEGILLPSTNENIITIKMKNGYNVGILKENVKNIEIIAKGEKPKYELPPLNIEKNEKLKTISILS
TGGTVASKVDYKTGAVHPSFTADDLIRAVPELLDIANIKGRAVMNILSENMKPEYWRKIAEEIKKEIEEGADGIVIAHGT
DTMSYTASALSFMVKADVPIILVGAQRSSDRPSSDAALNLISAVLAAREPIKGVYVVMHGESGDTFCYLHKGVKVRKCHS
SRRDAFKSINSIPVAKINPFTKEIIYLQEVEKSDNSKKVEINTNLEEKVALIKVYPGMDGEIIRFYVDKEYKGIVLEGTG
LGHAPEYIFEHIKYATDKGVVVVMTTQTINGRVNMNVYSNGRELQKLGVIGCEDMPPEVALVKLMYLLGNYEPEEVKKLI
NKNLVGEIEYRSRFDAY

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily

Homologues:

Organism=Homo sapiens, GI122937331, Length=291, Percent_Identity=32.3024054982818, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1788065, Length=351, Percent_Identity=32.4786324786325, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1789327, Length=323, Percent_Identity=31.2693498452012, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI193207372, Length=372, Percent_Identity=29.8387096774194, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI193207374, Length=372, Percent_Identity=29.8387096774194, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17558280, Length=372, Percent_Identity=29.8387096774194, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17558278, Length=372, Percent_Identity=29.8387096774194, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320527, Length=344, Percent_Identity=28.1976744186047, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323189, Length=303, Percent_Identity=28.0528052805281, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323187, Length=303, Percent_Identity=28.0528052805281, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323186, Length=303, Percent_Identity=28.0528052805281, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323184, Length=303, Percent_Identity=28.0528052805281, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24639609, Length=287, Percent_Identity=31.3588850174216, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645530, Length=282, Percent_Identity=30.8510638297872, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_METJA (Q60331)

Other databases:

- EMBL:   L77117
- PIR:   D64302
- RefSeq:   NP_246983.1
- ProteinModelPortal:   Q60331
- SMR:   Q60331
- GeneID:   1450858
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0020
- NMPDR:   fig|243232.1.peg.20
- TIGR:   MJ0020
- HOGENOM:   HBG497495
- OMA:   PEFYHDE
- ProtClustDB:   PRK04183
- BioCyc:   MJAN243232:MJ_0020-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00586
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519
- TIGRFAMs:   TIGR02153

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: 3.5.1.1

Molecular weight: Translated: 46344; Mature: 46344

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 84-84 ACT_SITE 160-160 ACT_SITE 161-161 ACT_SITE 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVGDVIRVETDKGIFEGILLPSTNENIITIKMKNGYNVGILKENVKNIEIIAKGEKPKY
CCCCCEEEEECCCCCCCEEECCCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCCCC
ELPPLNIEKNEKLKTISILSTGGTVASKVDYKTGAVHPSFTADDLIRAVPELLDIANIKG
CCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RAVMNILSENMKPEYWRKIAEEIKKEIEEGADGIVIAHGTDTMSYTASALSFMVKADVPI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEECCCCE
ILVGAQRSSDRPSSDAALNLISAVLAAREPIKGVYVVMHGESGDTFCYLHKGVKVRKCHS
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCEEEHHHH
SRRDAFKSINSIPVAKINPFTKEIIYLQEVEKSDNSKKVEINTNLEEKVALIKVYPGMDG
HHHHHHHHHCCCCCEEECCHHHHHHEEEHHHCCCCCEEEEEECCCCCEEEEEEEECCCCC
EIIRFYVDKEYKGIVLEGTGLGHAPEYIFEHIKYATDKGVVVVMTTQTINGRVNMNVYSN
CEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEEECC
GRELQKLGVIGCEDMPPEVALVKLMYLLGNYEPEEVKKLINKNLVGEIEYRSRFDAY
CCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEHHHCCCCC
>Mature Secondary Structure
MNVGDVIRVETDKGIFEGILLPSTNENIITIKMKNGYNVGILKENVKNIEIIAKGEKPKY
CCCCCEEEEECCCCCCCEEECCCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCCCC
ELPPLNIEKNEKLKTISILSTGGTVASKVDYKTGAVHPSFTADDLIRAVPELLDIANIKG
CCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RAVMNILSENMKPEYWRKIAEEIKKEIEEGADGIVIAHGTDTMSYTASALSFMVKADVPI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEECCCCE
ILVGAQRSSDRPSSDAALNLISAVLAAREPIKGVYVVMHGESGDTFCYLHKGVKVRKCHS
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCEEEHHHH
SRRDAFKSINSIPVAKINPFTKEIIYLQEVEKSDNSKKVEINTNLEEKVALIKVYPGMDG
HHHHHHHHHCCCCCEEECCHHHHHHEEEHHHCCCCCEEEEEECCCCCEEEEEEEECCCCC
EIIRFYVDKEYKGIVLEGTGLGHAPEYIFEHIKYATDKGVVVVMTTQTINGRVNMNVYSN
CEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEEECC
GRELQKLGVIGCEDMPPEVALVKLMYLLGNYEPEEVKKLINKNLVGEIEYRSRFDAY
CCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087