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The map label for this gene is gatE

Identifier: 15668190

GI number: 15668190

Start: 19229

End: 21121

Strand: Reverse

Name: gatE

Synonym: MJ0019

Alternate gene names: 15668190

Gene position: 21121-19229 (Counterclockwise)

Preceding gene: 15668191

Following gene: 15668187

Centisome position: 1.27

GC content: 34.39

Gene sequence:

>1893_bases
ATGGAGATTAACTATGAAAAAGTTGGTTTAAAGGTTGGGTTAGAAATTCATCAACAGTTAAATACAAAGAGAAAGTTATT
CTGCCACTGTCCAACAATTTTAAGAGATGATGAACCAGATGGAGAGATTGTTAGAGTTTTAAGACCTTCATTAAGTGAAA
TGGGAGAAGTTGATAGAGCTGCTTTAATAGAGGCAAGGAAAGGGAAGAAATTCATTTATCAATTTTATAATGACACAACA
TGTTTGGTTGAGTTGGATGAAGAACCTCCACATCCACCAAGTGAAGAGGCTTTAAGGATAGCGTTAGAGGTTGCTTTATT
GATGAATATGAACGTGGTTGATGTTGCATACACAATGAGAAAGATAGTTATTGACGGTTCAAACACTTCTGGATTTCAAA
GAACCATATTTTTAGCAAGAGATGGATATATAGAAACATCTGAGGGAAAAGTAGGAATAACAAGCTTATGTTTAGAGGAA
GATGCTGCAAGAAAGATAGAAGATAGAGGGGATGCAGTTGTTTATAACTTAGATAGGTTGGGAATTCCATTGGTTGAGAT
TTCAACAGCTCCCGACATAAAGACTCCAAAGATGGCTAAAGAGGCAGCAAGAAGAATTGGAGAGATATTAAGAGCCACTG
GAAAGGTTAAGAGAGGTTTAGGGACTATAAGGCAGGATATAAACATATCAATTAAAGATGGAGCAAGAATAGAGGTTAAG
GGAGTTCAAGACTTAGATTTAATTGAAAAGGTTGTAGAGAATGAAGTTATAAGGCAACTAAACTTATTAAAGATTAGAGA
TGAATTAAGAGAAAGAAATGCAGAGGTTGTTGAGAAGATATTTGATGTTACAGAGATATTTAAAGACTGTAAATCAAAAA
TTATACAAAATGCTTTAAAGAAAAAGAATGGAAAGGTTAAGGCAGTTTTATTAAAAGGATTTGCTGGTTTAGTTGGAAAA
GAGATTCAGCCAGGAAGAAGATTAGGAACTGAATTTTCAGATAGGGCCAAGGTTATAGCTGGCGTTGGAGGGCTTTTCCA
CACTGATGAGTTGCCAAAATATGGTATTACAGAGGAGGAAGTTAAAAAACTTAAAGAGTTTGTTAATGCAGAAGAAAATG
ATGCTGTAATTATTGTTGCAGATGAGGAAAGCAAGGTAGATAGGGCGTTAGAGGCTGTAATAGAGAGGGCTAAAGAGGCA
TTAATAGGAGTTCCAGAGGAAACAAGAAGGGCTTTAGAGGATGGAAATACTGCATATCTAAGACCGCTACCTGGAGCCGC
AAGAATGTATCCTGAAACCGATATCCCACCAATAATTATAAAGAAGGAGTTTATTGAGGAGATTAGAGCTAATCTGCCAG
AACTTCCAGAGGAGAAGTTTGAGAGGTTTAAGAAAGAATATAAATTAAATGATGAATTAGCTAAAAAGATGGTTTTAAGT
TATTACGTTGATTTATTTGAAGATCTATGTAAGAAATTTAAGAATGTTAAGCCGGTTTTAATTGCTACAACCTTAGAAGG
GACATTGAAGGAGATTAAAAGAGAAGGATATGATATTGATAAGTTGGAGGATAGACATTTAGAAGAGACCTTTAAAGCTC
TATCTGAAGGTAAGATAGCTAAAGAGGGAATTGTTGAGGTTTTAAAAGGCTTTTGTGAGTTTCCAGATAAAAGTATAGAT
GAGATTTTAGAAATTAAAGGATTAAAAGGATTATCTAAGGAAGAAGTTGAAAAGATTATTGAGGGCATAATTAAAGAACA
TTTAAATGTGGTTAAAGAAAAAGGAGAAAAGGCCTATGGATTTTTAATGGGTAGATGTATGGCAAAGCTAAGAGGAAAAG
CGGATGGAAAGTTAGTTAATGATATATTGAGAAAAAAGTTAAAGGAGATTTAA

Upstream 100 bases:

>100_bases
AGGAAGTTAAAAAATTAATTAATAAGAATTTGGTTGGGGAGATTGAATATAGGAGCAGATTTGATGCATACTAATAATAA
CAAATTAATTTGGTGATAAC

Downstream 100 bases:

>100_bases
CTCCATTTCTTATAATTACTCTTTTTGATTACTAACTTCTTATAGTATTTTCCATTTACATAAGACTTTTTTACAATTAT
TTTTCCTTTTCCTGAAATTT

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MEINYEKVGLKVGLEIHQQLNTKRKLFCHCPTILRDDEPDGEIVRVLRPSLSEMGEVDRAALIEARKGKKFIYQFYNDTT
CLVELDEEPPHPPSEEALRIALEVALLMNMNVVDVAYTMRKIVIDGSNTSGFQRTIFLARDGYIETSEGKVGITSLCLEE
DAARKIEDRGDAVVYNLDRLGIPLVEISTAPDIKTPKMAKEAARRIGEILRATGKVKRGLGTIRQDINISIKDGARIEVK
GVQDLDLIEKVVENEVIRQLNLLKIRDELRERNAEVVEKIFDVTEIFKDCKSKIIQNALKKKNGKVKAVLLKGFAGLVGK
EIQPGRRLGTEFSDRAKVIAGVGGLFHTDELPKYGITEEEVKKLKEFVNAEENDAVIIVADEESKVDRALEAVIERAKEA
LIGVPEETRRALEDGNTAYLRPLPGAARMYPETDIPPIIIKKEFIEEIRANLPELPEEKFERFKKEYKLNDELAKKMVLS
YYVDLFEDLCKKFKNVKPVLIATTLEGTLKEIKREGYDIDKLEDRHLEETFKALSEGKIAKEGIVEVLKGFCEFPDKSID
EILEIKGLKGLSKEEVEKIIEGIIKEHLNVVKEKGEKAYGFLMGRCMAKLRGKADGKLVNDILRKKLKEI

Sequences:

>Translated_630_residues
MEINYEKVGLKVGLEIHQQLNTKRKLFCHCPTILRDDEPDGEIVRVLRPSLSEMGEVDRAALIEARKGKKFIYQFYNDTT
CLVELDEEPPHPPSEEALRIALEVALLMNMNVVDVAYTMRKIVIDGSNTSGFQRTIFLARDGYIETSEGKVGITSLCLEE
DAARKIEDRGDAVVYNLDRLGIPLVEISTAPDIKTPKMAKEAARRIGEILRATGKVKRGLGTIRQDINISIKDGARIEVK
GVQDLDLIEKVVENEVIRQLNLLKIRDELRERNAEVVEKIFDVTEIFKDCKSKIIQNALKKKNGKVKAVLLKGFAGLVGK
EIQPGRRLGTEFSDRAKVIAGVGGLFHTDELPKYGITEEEVKKLKEFVNAEENDAVIIVADEESKVDRALEAVIERAKEA
LIGVPEETRRALEDGNTAYLRPLPGAARMYPETDIPPIIIKKEFIEEIRANLPELPEEKFERFKKEYKLNDELAKKMVLS
YYVDLFEDLCKKFKNVKPVLIATTLEGTLKEIKREGYDIDKLEDRHLEETFKALSEGKIAKEGIVEVLKGFCEFPDKSID
EILEIKGLKGLSKEEVEKIIEGIIKEHLNVVKEKGEKAYGFLMGRCMAKLRGKADGKLVNDILRKKLKEI
>Mature_630_residues
MEINYEKVGLKVGLEIHQQLNTKRKLFCHCPTILRDDEPDGEIVRVLRPSLSEMGEVDRAALIEARKGKKFIYQFYNDTT
CLVELDEEPPHPPSEEALRIALEVALLMNMNVVDVAYTMRKIVIDGSNTSGFQRTIFLARDGYIETSEGKVGITSLCLEE
DAARKIEDRGDAVVYNLDRLGIPLVEISTAPDIKTPKMAKEAARRIGEILRATGKVKRGLGTIRQDINISIKDGARIEVK
GVQDLDLIEKVVENEVIRQLNLLKIRDELRERNAEVVEKIFDVTEIFKDCKSKIIQNALKKKNGKVKAVLLKGFAGLVGK
EIQPGRRLGTEFSDRAKVIAGVGGLFHTDELPKYGITEEEVKKLKEFVNAEENDAVIIVADEESKVDRALEAVIERAKEA
LIGVPEETRRALEDGNTAYLRPLPGAARMYPETDIPPIIIKKEFIEEIRANLPELPEEKFERFKKEYKLNDELAKKMVLS
YYVDLFEDLCKKFKNVKPVLIATTLEGTLKEIKREGYDIDKLEDRHLEETFKALSEGKIAKEGIVEVLKGFCEFPDKSID
EILEIKGLKGLSKEEVEKIIEGIIKEHLNVVKEKGEKAYGFLMGRCMAKLRGKADGKLVNDILRKKLKEI

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily

Homologues:

Organism=Saccharomyces cerevisiae, GI6319391, Length=279, Percent_Identity=24.7311827956989, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATE_METJA (Q60325)

Other databases:

- EMBL:   L77117
- PIR:   C64302
- RefSeq:   NP_246982.1
- ProteinModelPortal:   Q60325
- GeneID:   1450857
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0019
- NMPDR:   fig|243232.1.peg.19
- TIGR:   MJ0019
- HOGENOM:   HBG297405
- OMA:   TSGFQRT
- ProtClustDB:   PRK04028
- BioCyc:   MJAN243232:MJ_0019-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00588
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00134

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 71226; Mature: 71226

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINYEKVGLKVGLEIHQQLNTKRKLFCHCPTILRDDEPDGEIVRVLRPSLSEMGEVDRA
CCCCHHHHHHHHHHHHHHHHCCCHHEEEECCHHHCCCCCCHHHHHHHHHHHHHHCCHHHH
ALIEARKGKKFIYQFYNDTTCLVELDEEPPHPPSEEALRIALEVALLMNMNVVDVAYTMR
HHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
KIVIDGSNTSGFQRTIFLARDGYIETSEGKVGITSLCLEEDAARKIEDRGDAVVYNLDRL
HHEEECCCCCCCEEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHC
GIPLVEISTAPDIKTPKMAKEAARRIGEILRATGKVKRGLGTIRQDINISIKDGARIEVK
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEC
GVQDLDLIEKVVENEVIRQLNLLKIRDELRERNAEVVEKIFDVTEIFKDCKSKIIQNALK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKNGKVKAVLLKGFAGLVGKEIQPGRRLGTEFSDRAKVIAGVGGLFHTDELPKYGITEEE
HCCCCEEEHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCCCHHH
VKKLKEFVNAEENDAVIIVADEESKVDRALEAVIERAKEALIGVPEETRRALEDGNTAYL
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEE
RPLPGAARMYPETDIPPIIIKKEFIEEIRANLPELPEEKFERFKKEYKLNDELAKKMVLS
ECCCCCHHCCCCCCCCCEEECHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
YYVDLFEDLCKKFKNVKPVLIATTLEGTLKEIKREGYDIDKLEDRHLEETFKALSEGKIA
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCH
KEGIVEVLKGFCEFPDKSIDEILEIKGLKGLSKEEVEKIIEGIIKEHLNVVKEKGEKAYG
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
FLMGRCMAKLRGKADGKLVNDILRKKLKEI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEINYEKVGLKVGLEIHQQLNTKRKLFCHCPTILRDDEPDGEIVRVLRPSLSEMGEVDRA
CCCCHHHHHHHHHHHHHHHHCCCHHEEEECCHHHCCCCCCHHHHHHHHHHHHHHCCHHHH
ALIEARKGKKFIYQFYNDTTCLVELDEEPPHPPSEEALRIALEVALLMNMNVVDVAYTMR
HHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
KIVIDGSNTSGFQRTIFLARDGYIETSEGKVGITSLCLEEDAARKIEDRGDAVVYNLDRL
HHEEECCCCCCCEEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHC
GIPLVEISTAPDIKTPKMAKEAARRIGEILRATGKVKRGLGTIRQDINISIKDGARIEVK
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEC
GVQDLDLIEKVVENEVIRQLNLLKIRDELRERNAEVVEKIFDVTEIFKDCKSKIIQNALK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKNGKVKAVLLKGFAGLVGKEIQPGRRLGTEFSDRAKVIAGVGGLFHTDELPKYGITEEE
HCCCCEEEHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCCCHHH
VKKLKEFVNAEENDAVIIVADEESKVDRALEAVIERAKEALIGVPEETRRALEDGNTAYL
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEE
RPLPGAARMYPETDIPPIIIKKEFIEEIRANLPELPEEKFERFKKEYKLNDELAKKMVLS
ECCCCCHHCCCCCCCCCEEECHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
YYVDLFEDLCKKFKNVKPVLIATTLEGTLKEIKREGYDIDKLEDRHLEETFKALSEGKIA
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCH
KEGIVEVLKGFCEFPDKSIDEILEIKGLKGLSKEEVEKIIEGIIKEHLNVVKEKGEKAYG
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
FLMGRCMAKLRGKADGKLVNDILRKKLKEI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087