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The map label for this gene is rffG

Identifier: 30995408

GI number: 30995408

Start: 925683

End: 926696

Strand: Direct

Name: rffG

Synonym: HI0873

Alternate gene names: 30995408

Gene position: 925683-926696 (Clockwise)

Preceding gene: 16272813

Following gene: 16272816

Centisome position: 50.58

GC content: 37.77

Gene sequence:

>1014_bases
ATGAATATTTTAGTCACTGGCGGTTCTGGCTTCATCGGTTCCGCTCTTATTCGATATATCATTAATCATACTCAAGATTT
CGTTATCAATATTGATAAGCTGACCTATGCAGCAAATCAATCAGCCTTGAGAGAGGTAGAAAATAATCCTCGTTATGTCT
TTGAAAAAGTTGATATCTGTGATCTTAACGTAATAGAAAACATTTTCGAAAAATATCAGCCTGATGCGGTGATGCATTTG
GCGGCAGAAAGTCATGTCGATCGTTCTATTTCAGGAGCGGCTGATTTTGTTCAAACGAATATTGTTGGTACTTATACGTT
ATTAGAGGTCGCTAAGAATTATTGGCATACTTTAGACGAGGCTAAAAAAACGACCTTTCGATTTCACCATATTTCTACAG
ATGAAGTGTATGGGGATTTATCCCTTTCTGAGCCTGCCTTTACCGAACAGTCTCCTTATCATCCTAGCAGCCCTTATTCA
GCCTCAAAAGCCGCAAGTAATCATCTAGTACAGGCTTGGCATCGCACTTATGGTTTACCAGTGATTATCACAAACAGTTC
AAATAACTATGGGGCTTATCAGCATGCTGAAAAGCTCATCCCTTTAATGATTTCAAATGCTGTGATGGGAAAGCCTTTGC
CAATTTATGGTGATGGGCAGCAGATTCGTGATTGGTTATTTGTGGAAGATCATGTTCAAGCTTCATATTTAGTTTTGACA
AAAGGTCGAGTCGGAGAAAACTATAATATTGGTGGAAATTGTGAAAAAACAAACCTTGAAGTGGTTAAAAGAATTTGCCA
ATTACTCGAAGAACTTGCGCCAAGTAAGCCTAATCACATTAAGTATTACGAAGATTTAATGACTTTTGTAAAAGACCGAC
CTGGGCATGATGTGCGATATTCATTGGATTGTTCTAAAATTCATGCAGAATTAGGTTGGCAACCGCAAATAACCTTTGAA
CAAGGACTTCGTCAGACGGTAAAATGGTATTTGTTTAATAGTTCATCTTCTTAA

Upstream 100 bases:

>100_bases
ACAATGAATGTGGTTTTAAATCGTGATGGAGCTTATTAACGTTATTTCTTAAGTGAGATTTCTTCTATTTGCATTACCTT
AAATTTATTAACCCAACATG

Downstream 100 bases:

>100_bases
ATAGGGTAAACGATAAGAATGTTTATCTATCTTGTAATTTTTTCTCTTTTTATTAGACAATAAAACACAACATAGCTGAA
AAATAAAAAATATTGCCTGA

Product: dTDP-glucose 46-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNILVTGGSGFIGSALIRYIINHTQDFVINIDKLTYAANQSALREVENNPRYVFEKVDICDLNVIENIFEKYQPDAVMHL
AAESHVDRSISGAADFVQTNIVGTYTLLEVAKNYWHTLDEAKKTTFRFHHISTDEVYGDLSLSEPAFTEQSPYHPSSPYS
ASKAASNHLVQAWHRTYGLPVIITNSSNNYGAYQHAEKLIPLMISNAVMGKPLPIYGDGQQIRDWLFVEDHVQASYLVLT
KGRVGENYNIGGNCEKTNLEVVKRICQLLEELAPSKPNHIKYYEDLMTFVKDRPGHDVRYSLDCSKIHAELGWQPQITFE
QGLRQTVKWYLFNSSSS

Sequences:

>Translated_337_residues
MNILVTGGSGFIGSALIRYIINHTQDFVINIDKLTYAANQSALREVENNPRYVFEKVDICDLNVIENIFEKYQPDAVMHL
AAESHVDRSISGAADFVQTNIVGTYTLLEVAKNYWHTLDEAKKTTFRFHHISTDEVYGDLSLSEPAFTEQSPYHPSSPYS
ASKAASNHLVQAWHRTYGLPVIITNSSNNYGAYQHAEKLIPLMISNAVMGKPLPIYGDGQQIRDWLFVEDHVQASYLVLT
KGRVGENYNIGGNCEKTNLEVVKRICQLLEELAPSKPNHIKYYEDLMTFVKDRPGHDVRYSLDCSKIHAELGWQPQITFE
QGLRQTVKWYLFNSSSS
>Mature_337_residues
MNILVTGGSGFIGSALIRYIINHTQDFVINIDKLTYAANQSALREVENNPRYVFEKVDICDLNVIENIFEKYQPDAVMHL
AAESHVDRSISGAADFVQTNIVGTYTLLEVAKNYWHTLDEAKKTTFRFHHISTDEVYGDLSLSEPAFTEQSPYHPSSPYS
ASKAASNHLVQAWHRTYGLPVIITNSSNNYGAYQHAEKLIPLMISNAVMGKPLPIYGDGQQIRDWLFVEDHVQASYLVLT
KGRVGENYNIGGNCEKTNLEVVKRICQLLEELAPSKPNHIKYYEDLMTFVKDRPGHDVRYSLDCSKIHAELGWQPQITFE
QGLRQTVKWYLFNSSSS

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=39.4578313253012, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=25.5255255255255, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI4504031, Length=353, Percent_Identity=24.3626062322946, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI48994969, Length=334, Percent_Identity=66.1676646706587, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=62.280701754386, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1788366, Length=354, Percent_Identity=25.1412429378531, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1786974, Length=354, Percent_Identity=25.9887005649718, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=37.0481927710843, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=35.9516616314199, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17539532, Length=333, Percent_Identity=22.5225225225225, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=362, Percent_Identity=25.9668508287293, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=364, Percent_Identity=25.8241758241758, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=354, Percent_Identity=23.1638418079096, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=354, Percent_Identity=23.1638418079096, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=354, Percent_Identity=23.1638418079096, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=354, Percent_Identity=23.1638418079096, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=354, Percent_Identity=23.1638418079096, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=348, Percent_Identity=22.1264367816092, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=356, Percent_Identity=26.4044943820225, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=25.4847645429363, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21356223, Length=333, Percent_Identity=24.6246246246246, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24158427, Length=345, Percent_Identity=22.3188405797101, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RMLB_HAEIN (P44914)

Other databases:

- EMBL:   L42023
- PIR:   C64099
- RefSeq:   NP_439034.2
- ProteinModelPortal:   P44914
- SMR:   P44914
- GeneID:   949510
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0873
- NMPDR:   fig|71421.1.peg.841
- TIGR:   HI_0873
- HOGENOM:   HBG755066
- OMA:   DVHITVL
- ProtClustDB:   CLSK824762
- BioCyc:   HINF71421:HI_0873-MONOMER
- BRENDA:   4.2.1.46
- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01181

Pfam domain/function: PF01370 Epimerase

EC number: =4.2.1.46

Molecular weight: Translated: 38198; Mature: 38198

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 135-135 ACT_SITE 136-136 ACT_SITE 160-160 BINDING 81-81 BINDING 85-85 BINDING 100-100 BINDING 189-189 BINDING 190-190 BINDING 224-224 BINDING 259-259

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGGSGFIGSALIRYIINHTQDFVINIDKLTYAANQSALREVENNPRYVFEKVDIC
CEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHCHHHHHHHHCCCCEEEEECCEE
DLNVIENIFEKYQPDAVMHLAAESHVDRSISGAADFVQTNIVGTYTLLEVAKNYWHTLDE
CHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKTTFRFHHISTDEVYGDLSLSEPAFTEQSPYHPSSPYSASKAASNHLVQAWHRTYGLP
HHHHEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
VIITNSSNNYGAYQHAEKLIPLMISNAVMGKPLPIYGDGQQIRDWLFVEDHVQASYLVLT
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEECCCCEEEEEEE
KGRVGENYNIGGNCEKTNLEVVKRICQLLEELAPSKPNHIKYYEDLMTFVKDRPGHDVRY
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEE
SLDCSKIHAELGWQPQITFEQGLRQTVKWYLFNSSSS
ECCHHHHHHHCCCCCCCHHHHHHHHHEEEEEEECCCC
>Mature Secondary Structure
MNILVTGGSGFIGSALIRYIINHTQDFVINIDKLTYAANQSALREVENNPRYVFEKVDIC
CEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHCHHHHHHHHCCCCEEEEECCEE
DLNVIENIFEKYQPDAVMHLAAESHVDRSISGAADFVQTNIVGTYTLLEVAKNYWHTLDE
CHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKTTFRFHHISTDEVYGDLSLSEPAFTEQSPYHPSSPYSASKAASNHLVQAWHRTYGLP
HHHHEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
VIITNSSNNYGAYQHAEKLIPLMISNAVMGKPLPIYGDGQQIRDWLFVEDHVQASYLVLT
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEECCCCEEEEEEE
KGRVGENYNIGGNCEKTNLEVVKRICQLLEELAPSKPNHIKYYEDLMTFVKDRPGHDVRY
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEE
SLDCSKIHAELGWQPQITFEQGLRQTVKWYLFNSSSS
ECCHHHHHHHCCCCCCCHHHHHHHHHEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800