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The map label for this gene is nudF

Identifier: 30995372

GI number: 30995372

Start: 420086

End: 420718

Strand: Direct

Name: nudF

Synonym: HI0398

Alternate gene names: 30995372

Gene position: 420086-420718 (Clockwise)

Preceding gene: 16272346

Following gene: 16272348

Centisome position: 22.95

GC content: 41.55

Gene sequence:

>633_bases
ATGTCAGAAATTCAACATTTTAGCCAACAAGATATAGAAATATTGGGCGAACAAACTTTGTATGAAGGTTTTTTCACGCT
TAAACGAATCCAATTTAAACATAAACTTTTTGCTGGGGGACAAAGTGGCGTCGTAACCCGAGAATTACTGATTAAAGGTG
CCGCCTCAGCGGTTATCGCTTATGATCCAAAAGAAGATTCGGTGATTTTAGTCGAACAAGTTCGTATTGGCGCGGCTTAT
CATCCTGAATCTCATCGTTCGCCTTGGTTACTTGAACTCATTGCAGGTATGGTGGAAAAAGGCGAAAAGCCAGAAGACGT
TGCTTTGCGTGAGAGTGAAGAAGAAGCAGGGATTCAAGTCAAAAATTTAACACATTGTTTAAGTGTGTGGGATAGCCCAG
GTGGCATAGTGGAGCGGATTCATTTATTTGCAGGCGAAGTGGATAGCGCACAAGCAAAAGGCATTCACGGTTTGGCTGAA
GAAAATGAAGACATTAAAGTGCACGTAGTAAAACGTGAACAGGCGTATCAATGGATGTGCGAGGGGAAAATTGATAATGG
CATTGCAGTGATTGGATTGCAGTGGCTTCAATTAAATTATGCTCAGTTACAACAAAGTTGGAAACGTAGCTAG

Upstream 100 bases:

>100_bases
TTTGTGACTCACTTCAAACTCTCAACAAAAAGTGGAAACCTTCTATTTTTTTTGTAGTCTTTTAAGCGTATGATTTTGCG
TGCAGTTTTGGAGAAAACAG

Downstream 100 bases:

>100_bases
GGGGCATTAATGAAAAATACGTTTGTTTATCAGGCGGAAAAGCCTGTTATAAAGTTATTACAAATTACCGATCCCCATTT
ATTTAAAGATGAAAGTGCGG

Product: hypothetical protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSEIQHFSQQDIEILGEQTLYEGFFTLKRIQFKHKLFAGGQSGVVTRELLIKGAASAVIAYDPKEDSVILVEQVRIGAAY
HPESHRSPWLLELIAGMVEKGEKPEDVALRESEEEAGIQVKNLTHCLSVWDSPGGIVERIHLFAGEVDSAQAKGIHGLAE
ENEDIKVHVVKREQAYQWMCEGKIDNGIAVIGLQWLQLNYAQLQQSWKRS

Sequences:

>Translated_210_residues
MSEIQHFSQQDIEILGEQTLYEGFFTLKRIQFKHKLFAGGQSGVVTRELLIKGAASAVIAYDPKEDSVILVEQVRIGAAY
HPESHRSPWLLELIAGMVEKGEKPEDVALRESEEEAGIQVKNLTHCLSVWDSPGGIVERIHLFAGEVDSAQAKGIHGLAE
ENEDIKVHVVKREQAYQWMCEGKIDNGIAVIGLQWLQLNYAQLQQSWKRS
>Mature_209_residues
SEIQHFSQQDIEILGEQTLYEGFFTLKRIQFKHKLFAGGQSGVVTRELLIKGAASAVIAYDPKEDSVILVEQVRIGAAYH
PESHRSPWLLELIAGMVEKGEKPEDVALRESEEEAGIQVKNLTHCLSVWDSPGGIVERIHLFAGEVDSAQAKGIHGLAEE
NEDIKVHVVKREQAYQWMCEGKIDNGIAVIGLQWLQLNYAQLQQSWKRS

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI1789412, Length=201, Percent_Identity=53.7313432835821, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1788810, Length=191, Percent_Identity=29.3193717277487, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ADPP_HAEIN (P44684)

Other databases:

- EMBL:   L42023
- PIR:   B64151
- RefSeq:   NP_438560.2
- ProteinModelPortal:   P44684
- SMR:   P44684
- GeneID:   949499
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0398
- NMPDR:   fig|71421.1.peg.373
- TIGR:   HI_0398
- HOGENOM:   HBG692398
- OMA:   YFPSPGG
- ProtClustDB:   CLSK870337
- BioCyc:   HINF71421:HI_0398-MONOMER
- BRENDA:   3.6.1.13
- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- Gene3D:   G3DSA:3.90.79.10
- TIGRFAMs:   TIGR00052

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: =3.6.1.13

Molecular weight: Translated: 23575; Mature: 23444

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: ACT_SITE 168-168 BINDING 82-82 BINDING 104-104 BINDING 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIQHFSQQDIEILGEQTLYEGFFTLKRIQFKHKLFAGGQSGVVTRELLIKGAASAVIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEE
YDPKEDSVILVEQVRIGAAYHPESHRSPWLLELIAGMVEKGEKPEDVALRESEEEAGIQV
ECCCCCCEEEEEEEEECCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCEEH
KNLTHCLSVWDSPGGIVERIHLFAGEVDSAQAKGIHGLAEENEDIKVHVVKREQAYQWMC
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEEEEECHHHHHHHC
EGKIDNGIAVIGLQWLQLNYAQLQQSWKRS
CCCCCCCEEEEEHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
SEIQHFSQQDIEILGEQTLYEGFFTLKRIQFKHKLFAGGQSGVVTRELLIKGAASAVIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEE
YDPKEDSVILVEQVRIGAAYHPESHRSPWLLELIAGMVEKGEKPEDVALRESEEEAGIQV
ECCCCCCEEEEEEEEECCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCEEH
KNLTHCLSVWDSPGGIVERIHLFAGEVDSAQAKGIHGLAEENEDIKVHVVKREQAYQWMC
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEEEEECHHHHHHHC
EGKIDNGIAVIGLQWLQLNYAQLQQSWKRS
CCCCCCCEEEEEHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10542272