Click here to switch to the map view.

The map label for this gene is metE [H]

Identifier: 16273589

GI number: 16273589

Start: 1771826

End: 1774096

Strand: Reverse

Name: metE [H]

Synonym: HI1702

Alternate gene names: 16273589

Gene position: 1774096-1771826 (Counterclockwise)

Preceding gene: 16273590

Following gene: 30995470

Centisome position: 96.94

GC content: 40.03

Gene sequence:

>2271_bases
ATGACAACATCACATATTTTAGGCTTTCCTCGTGTAGGGGCAAAACGTGAATTAAAATTCGCACAAGAACGTTATTGGCG
TAAAGAATTAGCAGAGCAAGATTTATTAGATTTAGCGAAAGCGTTGCGTGAAAAAAACTGGAAACATCAAGCAGCGGCGA
ATGCGGATTTCGTTGCAGTGGGCGATTTCACGTTCTACGATCATATTTTAGATTTACAAGTGGCAACAGGGGCAATTCCT
GCTCGTTTTGGTTTTGATAGCCAAAATTTAACCCTTGATCAATATTTCCAACTTGCACGTGGTAACAAAGATCAATTCGC
AATTGAAATGACCAAATGGTTTGATACGAACTATCACTATCTTGTGCCTGAGTTCCAAAAATCGACCGCTTTCAAAGCTA
ATCCAGCACATTATGTAAACCAAATCCGTGAAGCAAAAGCGTTAGGCTTAAACTTCAAACCTGTGATTGTTGGCCCATTA
ACATTCTTATGGTTAGGTAAAGAAAAAGGCGAAGCATTTAACCGTTTCGATTTATTAAATCAATTAGTGCCTGTTTATGT
TGAAATATTAAATGCATTAGTGGCTGAAGGGGCAGAGTGGATTCAAATTGATGAACCTGCATTAGCATTAGATTTACCAG
CAGAATGGGTTGAAGCCTATAAATCTGTTTACACTGAATTAAGCAAAGTGAACGCAAAATTATTATTAGCCACTTATTTT
GGTTCAGTTGCAGAACACGCTGAATTATTAAAAGCCTTACCTGTTGCAGGCTTGCATTTAGATTTAGTTCGTGCACCAGA
ACAACTTGCGGCATTTGAAGACTACAGCAAAGTGTTATCAGCTGGTGTGATTGAAGGTCGTAATATCTGGCGTGCAAACT
TAAACAAAGTGTTAGATGTATTAGAGCCATTAAAAGCAAAATTAGGTGAGCGTTTATGGATTGCACCAAGCTGTTCATTA
TTGCATACCCCATTTGATTTAGAAGTGGAAGTACAATTAAAAGAAAAAAATACCGCACTTTACAGCTGGTTATCTTTCAC
GCTACAAAAAGTAGAAGAATTAAACGTATTAAAACAAGCGTTAAATAATGGCAGAGCGTCTGTACAAGCTGCATTAGATG
CAAGCCAAGTGGCAGCAGATGCACGCGCAACGTCAAAAGAAATTCATCGCCCTGAAGTGGCAGAACGTTTAGCAAACTTG
CCAAAAGGTGCGGATCAACGTAAATCGCCATTTGCAGAACGTATCGTTAAGCAAAATGCGTGGTTAAATTTACCGCTTCT
GCCAACTACAAACATTGGTTCATTCCCACAAACTACTGAAATTCGTCACGCACGTGCAAGTTTCAAAAAAGGCGAGTTAT
CTCTTGCAGATTACGAAGCGGCAATGAAAAAAGAGATCGAATATGTAGTACGTCGCCAAGAAGAATTAGATTTAGATGTG
TTAGTTCACGGCGAAGCAGAACGTAACGACATGGTGGAATACTTTGGGGAATTATTAGATGGTTTCGCATTCACTAAATT
TGGTTGGGTACAAAGCTACGGTTCACGCTGTGTAAAACCACCAGTAATTTACGGTGATGTTACCCGCCCTGAGCCAATGA
CAGTACGTTGGTCTCAATATGCACAAAGTTTAACTAACCGTGTAATGAAAGGAATGCTCACAGGCCCTGTGACTATTTTA
CAATGGTCATTCGTGCGTAACGATATTCCACGTTCAACCGTATGTAAACAAATCGGCGTAGCATTATCTGATGAAGTGTT
AGATTTAGAAGCAGCAGGCATTAAAGTCATTCAAATTGACGAACCAGCCATTCGTGAAGGTTTACCGCTTAAACGTGCAG
ATTGGGACGCATACTTACAATGGGCAGGCGAAGCATTCCGTTTAAGCTCAATGGGCGTGCAAGATGATACGCAAATTCAC
ACTCACATGTGTTATTCCGAGTTTAACGACATCTTACCTGCCATTGCGGCATTAGATGCAGACGTGATTACCATCGAAAC
TTCACGTTCAGATATGGAATTATTAACGGCATTTGCAGATTTCAAATATCCAAATGATATTGGCCCAGGTGTTTATGATA
TTCACAGCCCTCGTGTGCCAACGGCAACGGAAGTAGAACATTTATTACGTAAAGCATTAAATGTAATTCCAAAAGAACGC
TTATGGGTAAACCCAGACTGCGGTTTAAAAACGCGTGGCTGGACAGAAACCATCGACCAATTAAAAGTGATGGTGGATGT
AACCAAAAAATTACGTGCGGAATTAGCGTAA

Upstream 100 bases:

>100_bases
ATAATAAGTTTGCATAATAAATGAAGATCAAGCATTCTATATTGGTATAGACGTTTAGCCGTCTAAATGATTAAAAAAAT
TACTTTAAATTGGAGTTATT

Downstream 100 bases:

>100_bases
GTCATAATACATAAAGAATAATTTTCAAACACAACATAATTAAAGCCGAATCTCTATGGGATTCGGTTTTTCTTATAGAT
AAAATGTTGAATATAATAAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MTTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIP
ARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHYLVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPL
TFLWLGKEKGEAFNRFDLLNQLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYTELSKVNAKLLLATYF
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDVLEPLKAKLGERLWIAPSCSL
LHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQALNNGRASVQAALDASQVAADARATSKEIHRPEVAERLANL
PKGADQRKSPFAERIVKQNAWLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTIL
QWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIH
THMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKER
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA

Sequences:

>Translated_756_residues
MTTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIP
ARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHYLVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPL
TFLWLGKEKGEAFNRFDLLNQLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYTELSKVNAKLLLATYF
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDVLEPLKAKLGERLWIAPSCSL
LHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQALNNGRASVQAALDASQVAADARATSKEIHRPEVAERLANL
PKGADQRKSPFAERIVKQNAWLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTIL
QWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIH
THMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKER
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA
>Mature_755_residues
TTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPA
RFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHYLVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPLT
FLWLGKEKGEAFNRFDLLNQLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYTELSKVNAKLLLATYFG
SVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDVLEPLKAKLGERLWIAPSCSLL
HTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQALNNGRASVQAALDASQVAADARATSKEIHRPEVAERLANLP
KGADQRKSPFAERIVKQNAWLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDVL
VHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTILQ
WSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHT
HMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKERL
WVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=757, Percent_Identity=54.4253632760898, Blast_Score=821, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=46.4516129032258, Blast_Score=676, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85239; Mature: 85108

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCEE
LVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPLTFLWLGKEKGEAFNRFDLLN
ECCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCCHHHHHHHHH
QLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYTELSKVNAKLLLATYF
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDV
HHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEPLKAKLGERLWIAPSCSLLHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQA
HHHHHHHHCCEEEECCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LNNGRASVQAALDASQVAADARATSKEIHRPEVAERLANLPKGADQRKSPFAERIVKQNA
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHCCC
WLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
CEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHHH
AQSLTNRVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQID
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHCCHHHEEECCCEEEEEEC
EPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDA
CHHHHCCCCCCCCCHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKER
CEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCEE
LVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPLTFLWLGKEKGEAFNRFDLLN
ECCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCCHHHHHHHHH
QLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYTELSKVNAKLLLATYF
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDV
HHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEPLKAKLGERLWIAPSCSLLHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQA
HHHHHHHHCCEEEECCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LNNGRASVQAALDASQVAADARATSKEIHRPEVAERLANLPKGADQRKSPFAERIVKQNA
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHCCC
WLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
CEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHHH
AQSLTNRVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQID
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHCCHHHEEECCCEEEEEEC
EPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDA
CHHHHCCCCCCCCCHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKER
CEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA