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The map label for this gene is ribE

Identifier: 16273503

GI number: 16273503

Start: 1679509

End: 1680123

Strand: Direct

Name: ribE

Synonym: HI1613

Alternate gene names: 16273503

Gene position: 1679509-1680123 (Clockwise)

Preceding gene: 16273499

Following gene: 16273505

Centisome position: 91.77

GC content: 37.24

Gene sequence:

>615_bases
ATGTTTACTGGAATTGTACAAGGCACCGCCCCCATCCACTCCATTAAAGAAAAGGCTAATTTTAGAACACAAGTGGTAAA
ATTACTACCTGAAATGCGTAAAGATCTGGAAATTGGCGCATCAATAGCAAATAACGGCGTATGTTTAACTGTAACTGAAA
TCAATGGTGATCTGGTTAGCTTTGATCTGATGCAAGAAACTTTAAAAATTACAAATCTCGGCACAGTAAAAGTGGGCGAT
TATGTGAATATAGAACGTGCTATGCAAATGGGAACGGAAATTGGCGGACATTTATTATCTGGTCATATTTATTGCACCGC
AAAAATTTCAGATATTATCGCTAGTGAAAATAACCGACAAATTTGGTTTGAACTGCCAAGTGCGGATGTGATGAAATATA
TTTTAACGAAAGGATTTGTTGCGGTAGATGGCATTAGCCTCACTATTGGCGAAGTAAGAGACACTCAATTCTGTGTAAAT
TTAATCCCTGAAACTTTACAACGAACCTTAATGGGGCGGCGTAAAGTGGGCGATATTGTGAATATCGAAATTGATCCACA
AACGCAAGCTATTGTAGATACGGTAGAAAATTATTTGCAGTCAAAAAATTTCTAG

Upstream 100 bases:

>100_bases
AATTTTAATCAACTTTTTAATATCAGCGTGGTATTGAGATAAAAGACGAAAATTCATAAATTTGATATACTGCTAAAAAT
TTTTAGAAAAGGAAAAAATT

Downstream 100 bases:

>100_bases
TTCATTTAAAAATATTAAGCAATTGAATAAAGTGAGAATGGGCATAAAGCCTATTCTCACATCATCAAGTGTTACTGCAA
CGCTTTCTCAATTTTTTCAA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MFTGIVQGTAPIHSIKEKANFRTQVVKLLPEMRKDLEIGASIANNGVCLTVTEINGDLVSFDLMQETLKITNLGTVKVGD
YVNIERAMQMGTEIGGHLLSGHIYCTAKISDIIASENNRQIWFELPSADVMKYILTKGFVAVDGISLTIGEVRDTQFCVN
LIPETLQRTLMGRRKVGDIVNIEIDPQTQAIVDTVENYLQSKNF

Sequences:

>Translated_204_residues
MFTGIVQGTAPIHSIKEKANFRTQVVKLLPEMRKDLEIGASIANNGVCLTVTEINGDLVSFDLMQETLKITNLGTVKVGD
YVNIERAMQMGTEIGGHLLSGHIYCTAKISDIIASENNRQIWFELPSADVMKYILTKGFVAVDGISLTIGEVRDTQFCVN
LIPETLQRTLMGRRKVGDIVNIEIDPQTQAIVDTVENYLQSKNF
>Mature_204_residues
MFTGIVQGTAPIHSIKEKANFRTQVVKLLPEMRKDLEIGASIANNGVCLTVTEINGDLVSFDLMQETLKITNLGTVKVGD
YVNIERAMQMGTEIGGHLLSGHIYCTAKISDIIASENNRQIWFELPSADVMKYILTKGFVAVDGISLTIGEVRDTQFCVN
LIPETLQRTLMGRRKVGDIVNIEIDPQTQAIVDTVENYLQSKNF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats

Homologues:

Organism=Escherichia coli, GI1787952, Length=202, Percent_Identity=65.8415841584158, Blast_Score=280, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6319733, Length=217, Percent_Identity=35.0230414746544, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISA_HAEIN (P45273)

Other databases:

- EMBL:   L42023
- PIR:   E64132
- RefSeq:   NP_439755.1
- ProteinModelPortal:   P45273
- SMR:   P45273
- GeneID:   950465
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1613
- NMPDR:   fig|71421.1.peg.1529
- TIGR:   HI_1613
- HOGENOM:   HBG289809
- OMA:   CCLTVTK
- ProtClustDB:   PRK09289
- BioCyc:   HINF71421:HI_1613-MONOMER
- BRENDA:   2.5.1.9
- InterPro:   IPR001783
- InterPro:   IPR017938
- PANTHER:   PTHR21098
- PIRSF:   PIRSF000498
- TIGRFAMs:   TIGR00187

Pfam domain/function: PF00677 Lum_binding; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =2.5.1.9

Molecular weight: Translated: 22590; Mature: 22590

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS51177 LUMAZINE_BIND; PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVQGTAPIHSIKEKANFRTQVVKLLPEMRKDLEIGASIANNGVCLTVTEINGDLVS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEH
FDLMQETLKITNLGTVKVGDYVNIERAMQMGTEIGGHLLSGHIYCTAKISDIIASENNRQ
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCEEEECEEEEEEEHHHHHHCCCCCE
IWFELPSADVMKYILTKGFVAVDGISLTIGEVRDTQFCVNLIPETLQRTLMGRRKVGDIV
EEEECCCHHHHHHHHHCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
NIEIDPQTQAIVDTVENYLQSKNF
EEEECCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFTGIVQGTAPIHSIKEKANFRTQVVKLLPEMRKDLEIGASIANNGVCLTVTEINGDLVS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEH
FDLMQETLKITNLGTVKVGDYVNIERAMQMGTEIGGHLLSGHIYCTAKISDIIASENNRQ
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCEEEECEEEEEEEHHHHHHCCCCCE
IWFELPSADVMKYILTKGFVAVDGISLTIGEVRDTQFCVNLIPETLQRTLMGRRKVGDIV
EEEECCCHHHHHHHHHCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
NIEIDPQTQAIVDTVENYLQSKNF
EEEECCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800