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The map label for this gene is hmrM

Identifier: 16273502

GI number: 16273502

Start: 1678071

End: 1679465

Strand: Reverse

Name: hmrM

Synonym: HI1612

Alternate gene names: 16273502

Gene position: 1679465-1678071 (Counterclockwise)

Preceding gene: 16273504

Following gene: 16273501

Centisome position: 91.77

GC content: 37.85

Gene sequence:

>1395_bases
ATGAATTTTCGTCTTTTATCTCAATACCACGCTGATATTAAAAAGTTGATTAAAATTTCCTTGCCTATTTTATTAGCACA
AATCGCACAAAACTCAATGGGATTAGCGGATACCATTATGGCGGGGCGAGTGAGTTCCACTGATATGGCAGCCATTTCTA
TTGGTGCTTCAATTTGGATGCCATTGATGTTTTTTGGGCAAGGTTTATTGTTGGCATTGCCGCCTACAATTTCTTATTTG
AATGGTTCAGGCCAACACCATCGCATTGCACATCAAGTTCGCCAAGGCATTTGGCTTGTGTTAGGCGTGAGTATTCCTTT
AGGTTTACTGATTTATTTCTGTGAAATTCCGCTGCAATATATGCAAATGGAAAGCAAAATGTCAGATTTAGCACGCGATT
ATTTACACGCGATGTTGTGGGGATTGCCAGCTTATTTGATGCTGATTAATTTTCGTTGTTTAAATGATGGGATTGAGAAA
ACCAAGCCTGCGATGGTCATTACCTTTTTAGGTTTATTGATTAATATTCCGCTTAATTACATTTTTATTTATGGAAAATT
TGGTATGCCTGCTTTTGGTGCGGTGGGCTGTGGTATTGCGACAGCTATTGTGAACTGGGCAATGTGCTTAATGATGATTT
TCTATTCCTACACGAATACTCAAGAACGTTCACTAAAAGTATTTAGTCAATTAATTGAAATGCCAAATCCGAAAACACTT
AAAAAATTACTGCGTTTAGGATTACCCATTGCCATTGCAATTTGTTGCGAAGTGGCGTTATATGCACTTACGTCCTTAAT
GCTTTCTCCGCTGGGTGCAACTATTGTGGCAAGCCATCAAATTACGCTGAATACTAGTTCTTTTATTTTTATGTTCCCTA
TGTCGATTGGTATGGCAACGACGATTTTAGTTGGACAAGCATTGGGTGCAGGTTCTCCACAAAATGCGAAGAAAATTGGC
TATGCCGCATTATTATTAGGGCTAACTGTGACAATTGTTACTGCATTAATTACGATTTTTTTCCGTTATGAAATTGCATC
GATCTTCGTGACAGATGAAATTGTCATCGCCATGGCAGCAAATCTATTATTATTTGCCGCACTTTATCAATTTTCAGATA
CCATTCAAATGGTGGTTGGTGGTATTTTACGTGGTTATAAAGATACTAAAGTCATTTTATACATTACCCTTTTCTCTTAT
TGGGTAATTGGTGTGCCACTTGGTTATACGCTAGGTCGTACAGATTGGCTTGTGCCACACATTGATGCGAAAGGTTTCTG
GATTGCCTTTGTGGTCTCGCTCACTTTTGCGGCATTCTTACTTTCTTTGAGAATGAAAAAAATGCAAGCTATGAACGACA
ACGCTATTTTACAACGTTTAGAAAAACTTAAATAA

Upstream 100 bases:

>100_bases
AGCCTTTTCTTTAATGGAGTGGATGGGGGCGGTGCCTTGTACAATTCCAGTAAACATAATTTTTTCCTTTTCTAAAAATT
TTTAGCAGTATATCAAATTT

Downstream 100 bases:

>100_bases
GGAACTTTATGCAACTTCCAGCTTTACAATCGGCGACATTAATTCGTCGTTACAAACGATTTCTTGCGGATATTGAATTG
CCAACTGGTGATGTTATGAC

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNFRLLSQYHADIKKLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWMPLMFFGQGLLLALPPTISYL
NGSGQHHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQYMQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIEK
TKPAMVITFLGLLINIPLNYIFIYGKFGMPAFGAVGCGIATAIVNWAMCLMMIFYSYTNTQERSLKVFSQLIEMPNPKTL
KKLLRLGLPIAIAICCEVALYALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMATTILVGQALGAGSPQNAKKIG
YAALLLGLTVTIVTALITIFFRYEIASIFVTDEIVIAMAANLLLFAALYQFSDTIQMVVGGILRGYKDTKVILYITLFSY
WVIGVPLGYTLGRTDWLVPHIDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMNDNAILQRLEKLK

Sequences:

>Translated_464_residues
MNFRLLSQYHADIKKLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWMPLMFFGQGLLLALPPTISYL
NGSGQHHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQYMQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIEK
TKPAMVITFLGLLINIPLNYIFIYGKFGMPAFGAVGCGIATAIVNWAMCLMMIFYSYTNTQERSLKVFSQLIEMPNPKTL
KKLLRLGLPIAIAICCEVALYALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMATTILVGQALGAGSPQNAKKIG
YAALLLGLTVTIVTALITIFFRYEIASIFVTDEIVIAMAANLLLFAALYQFSDTIQMVVGGILRGYKDTKVILYITLFSY
WVIGVPLGYTLGRTDWLVPHIDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMNDNAILQRLEKLK
>Mature_464_residues
MNFRLLSQYHADIKKLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWMPLMFFGQGLLLALPPTISYL
NGSGQHHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQYMQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIEK
TKPAMVITFLGLLINIPLNYIFIYGKFGMPAFGAVGCGIATAIVNWAMCLMMIFYSYTNTQERSLKVFSQLIEMPNPKTL
KKLLRLGLPIAIAICCEVALYALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMATTILVGQALGAGSPQNAKKIG
YAALLLGLTVTIVTALITIFFRYEIASIFVTDEIVIAMAANLLLFAALYQFSDTIQMVVGGILRGYKDTKVILYITLFSY
WVIGVPLGYTLGRTDWLVPHIDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMNDNAILQRLEKLK

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter. Confers resistance to norfloxacin, acriflavine, doxorubicin, ethidium bromide, tetraphenylphosphonium chloride, daunomycin, berberine and sodium deoxycholate

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Homologues:

Organism=Homo sapiens, GI22907060, Length=457, Percent_Identity=22.1006564551422, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI153792564, Length=451, Percent_Identity=21.0643015521064, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI38261960, Length=206, Percent_Identity=24.2718446601942, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI48994890, Length=458, Percent_Identity=46.943231441048, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI226510954, Length=421, Percent_Identity=22.3277909738717, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321821, Length=383, Percent_Identity=22.7154046997389, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320545, Length=428, Percent_Identity=20.3271028037383, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HMRM_HAEIN (P45272)

Other databases:

- EMBL:   L42023
- PIR:   B64173
- RefSeq:   NP_439754.1
- GeneID:   950466
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1612
- NMPDR:   fig|71421.1.peg.1528
- TIGR:   HI_1612
- HOGENOM:   HBG479615
- OMA:   HQIALNT
- ProtClustDB:   PRK01766
- BioCyc:   HINF71421:HI_1612-MONOMER
- InterPro:   IPR002528
- PANTHER:   PTHR11206
- TIGRFAMs:   TIGR00797

Pfam domain/function: PF01554 MatE

EC number: NA

Molecular weight: Translated: 51308; Mature: 51308

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xda073c4)-; HASH(0xe5513cc)-; HASH(0xe55948c)-; HASH(0xecf1690)-; HASH(0xdf866f0)-; HASH(0xcc588cc)-; HASH(0xea6e48c)-; HASH(0xdc39920)-; HASH(0xec67c44)-; HASH(0xec0c3f4)-; HASH(0xe12afb8)-; HASH(0xe19240c)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRLLSQYHADIKKLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
PLMFFGQGLLLALPPTISYLNGSGQHHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQY
HHHHHCCCCEEECCCHHHHCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHH
MQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIEKTKPAMVITFLGLLINIPLNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHCCCCE
IFIYGKFGMPAFGAVGCGIATAIVNWAMCLMMIFYSYTNTQERSLKVFSQLIEMPNPKTL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
KKLLRLGLPIAIAICCEVALYALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMAT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEHHHHHHHH
TILVGQALGAGSPQNAKKIGYAALLLGLTVTIVTALITIFFRYEIASIFVTDEIVIAMAA
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLLFAALYQFSDTIQMVVGGILRGYKDTKVILYITLFSYWVIGVPLGYTLGRTDWLVPH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCC
IDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMNDNAILQRLEKLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCH
>Mature Secondary Structure
MNFRLLSQYHADIKKLIKISLPILLAQIAQNSMGLADTIMAGRVSSTDMAAISIGASIWM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
PLMFFGQGLLLALPPTISYLNGSGQHHRIAHQVRQGIWLVLGVSIPLGLLIYFCEIPLQY
HHHHHCCCCEEECCCHHHHCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHH
MQMESKMSDLARDYLHAMLWGLPAYLMLINFRCLNDGIEKTKPAMVITFLGLLINIPLNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHCCCCE
IFIYGKFGMPAFGAVGCGIATAIVNWAMCLMMIFYSYTNTQERSLKVFSQLIEMPNPKTL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
KKLLRLGLPIAIAICCEVALYALTSLMLSPLGATIVASHQITLNTSSFIFMFPMSIGMAT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEHHHHHHHH
TILVGQALGAGSPQNAKKIGYAALLLGLTVTIVTALITIFFRYEIASIFVTDEIVIAMAA
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLLFAALYQFSDTIQMVVGGILRGYKDTKVILYITLFSYWVIGVPLGYTLGRTDWLVPH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCC
IDAKGFWIAFVVSLTFAAFLLSLRMKKMQAMNDNAILQRLEKLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800