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The map label for this gene is lolB [H]

Identifier: 16273497

GI number: 16273497

Start: 1673345

End: 1673974

Strand: Direct

Name: lolB [H]

Synonym: HI1607

Alternate gene names: 16273497

Gene position: 1673345-1673974 (Clockwise)

Preceding gene: 16273496

Following gene: 16273498

Centisome position: 91.43

GC content: 36.03

Gene sequence:

>630_bases
ATGAATAATATGAAAACCTTTAAATTTTTCACCGCACTTTTTGCCACTGCGATTCTAACCGCTTGCACATTAGATATGGA
ACGTCCTACTAATGTACAATACATTGATAAAACGGATGCGATATGGCAACAACATTTACAAAAGATTCAAAAAATTCAAT
CCTATCAAGCCAAAGGACAAATTGGTTATATCAGTCCAACAGAACGTTTTTCTAGCCGCTTTGAATGGCAATATCAAAAT
CCTAAATCTTATACGCTTAAACTCTATTCTTTAATCAGTAAATCGACTCTTTGGATTCAAATGCATCAAAGTGGGATGAC
AATTTCCGATAACAACGGCAATCAACAATCTGCAGCCAACTCCAAACTATTATTACAAGAGATTATTGGCATGGATGTGC
CATTAGAACATTTAGCTTATTGGTTGAAAGGCCAACCAGCAATGAACGCGGATTATCAAGTTGGCACCAACCATTTACTT
GGCGCATTTACTTATCATGTGGATGGTTCTCAATGGACGGCAGATTATCTAACCTATCACTCAAATAATTCGATGCCTGA
AAATATTTTGCTGAAAAATGACAGCACAAAACAAACCTTAAAAATTCGCGTAGACGAGTGGATTTACTAA

Upstream 100 bases:

>100_bases
CGGTTAAACAAACAAAAACGAATTACCCAACAAATTAACAAATAAAAGTAACTTTACTCAATTAAACTAAATCCTTAAAA
TATGCGAGCTTATTAACTTG

Downstream 100 bases:

>100_bases
TGAATACATTAATGAAATCACATCAATTTTCTACCGCACTTTGCCAAAATACAACAGAATCAAATGGGCAGCCATTACGC
TTCCCTAGCCCAGCCAAACT

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MNNMKTFKFFTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQIGYISPTERFSSRFEWQYQN
PKSYTLKLYSLISKSTLWIQMHQSGMTISDNNGNQQSAANSKLLLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLL
GAFTYHVDGSQWTADYLTYHSNNSMPENILLKNDSTKQTLKIRVDEWIY

Sequences:

>Translated_209_residues
MNNMKTFKFFTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQIGYISPTERFSSRFEWQYQN
PKSYTLKLYSLISKSTLWIQMHQSGMTISDNNGNQQSAANSKLLLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLL
GAFTYHVDGSQWTADYLTYHSNNSMPENILLKNDSTKQTLKIRVDEWIY
>Mature_209_residues
MNNMKTFKFFTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQIGYISPTERFSSRFEWQYQN
PKSYTLKLYSLISKSTLWIQMHQSGMTISDNNGNQQSAANSKLLLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLL
GAFTYHVDGSQWTADYLTYHSNNSMPENILLKNDSTKQTLKIRVDEWIY

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein [H]

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family [H]

Homologues:

Organism=Escherichia coli, GI1787460, Length=213, Percent_Identity=26.7605633802817, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004565 [H]

Pfam domain/function: PF03550 LolB [H]

EC number: NA

Molecular weight: Translated: 24194; Mature: 24194

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMKTFKFFTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCC
IGYISPTERFSSRFEWQYQNPKSYTLKLYSLISKSTLWIQMHQSGMTISDNNGNQQSAAN
EEECCCHHHHHHHCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCHHHH
SKLLLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLLGAFTYHVDGSQWTADYLTYH
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEHEEEC
SNNSMPENILLKNDSTKQTLKIRVDEWIY
CCCCCCCEEEEECCCCCEEEEEEECCCCC
>Mature Secondary Structure
MNNMKTFKFFTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCC
IGYISPTERFSSRFEWQYQNPKSYTLKLYSLISKSTLWIQMHQSGMTISDNNGNQQSAAN
EEECCCHHHHHHHCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCHHHH
SKLLLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLLGAFTYHVDGSQWTADYLTYH
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEHEEEC
SNNSMPENILLKNDSTKQTLKIRVDEWIY
CCCCCCCEEEEECCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA