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The map label for this gene is cca [H]

Identifier: 16273496

GI number: 16273496

Start: 1672032

End: 1673282

Strand: Direct

Name: cca [H]

Synonym: HI1606

Alternate gene names: 16273496

Gene position: 1672032-1673282 (Clockwise)

Preceding gene: 16273495

Following gene: 16273497

Centisome position: 91.36

GC content: 39.41

Gene sequence:

>1251_bases
ATGAAAGTTTATTTGGTTGGAGGGGCTGTTCGTGATCAATTATTGGGGTTACCCGTAAAAGATCGTGATTGGATTGTGGT
AGGCGCAGACCCCGCTACGCTACTCTCTCTCGGTTATCAGCAAGTAGGCAAAGATTTTCCAGTTTTTCTCAATCCTAAAA
CAAAAGAAGAATATGCGCTTGCCCGAACAGAACGTAAATCTAGCGCAGGTTACACGGGCTTTATTTGTGATTTCTCCCCA
ACAATCACATTAGAACAAGATTTAATTCGACGAGATCTCACTATTAACGCCATGGCTCAAAGCGAAGACGGTGAAATTAT
CGATCCTTATGGTGGAAAACAAGATTTAGAAAATCGAATTTTGCGCCATATTTCTCCCGCTTTTTCAGAAGATCCTTTAC
GAGTATTGCGCGTGGCACGCTTTGCTGCCCGTTATCATTCCCTCGGTTTTAAAATCGCCTCGGAAACATTAGCTCTAATG
GCAGAACTTGCCCAATCAGGAGAATTACAACATCTCACGGCGGAACGCATTTGGCTAGAAACAGAAAAAGCCTTAAACGA
AAAAAATCCTGAAATTTATTTTGAAACCTTACATAAAACAGGCGCATTAAGCGTTTTATTTTCTGAAATCGATGCCCTTT
ATGGCGTACCAAATCCAGTAAAACATCATCCCGAAGTGGATAGTTTTATTCATACCATGTTGGTATTAAAACAAGCGGTT
AATTTGACTGAAAATAACCCTATCCTAAATAAAAGTGCGGTTCGTTTTGCCGCAATTTGTCACGATCTTGGCAAGGCTCT
TACACCTCAAAATATTCTTCCTCATCATTATGGGCATGAACAAGCTGGCATAAAACCGACTAGATCATTGTGCAAACGCT
TGAAGGTTCCAAGTTATTTTCAAGAACTTGCAGAACTGACTTGTGAATTTCACACGCATATTCACAAAGCCTTTGAGCTT
CGAGCTGAAACCGTCATAACGCTGTTTAATCGTTTTGATGTGTGGAGAAAACCACAACGCTTTCAAGAATTTCTACAAGT
ATGTCTCGCTGATACTCGAGGTAGAACAGGTTTTGAAACTAAAGACTATCCACAAATAGATTACATCAATCAATTATTAC
ATGCGGCAAATGAAGTAGATGTACAGCAAGTTATTGCCGATGGATTTGAGAAACAAGCAATCAAAAATGAATTGACGAAA
CGAAGAATCTTAGCGGTTAAACAAACAAAAACGAATTACCCAACAAATTAA

Upstream 100 bases:

>100_bases
TTATGGCGGTTCAGTATTGGGCGTAGGCTTACTCTTCGGCTTGCTCATTCCTTATGTGTTACCAAAACGTCGCCGTCGTG
ATGGCTGGGCATAATCACGA

Downstream 100 bases:

>100_bases
CAAATAAAAGTAACTTTACTCAATTAAACTAAATCCTTAAAATATGCGAGCTTATTAACTTGATGAATAATATGAAAACC
TTTAAATTTTTCACCGCACT

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKVYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYALARTERKSSAGYTGFICDFSP
TITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRILRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALM
AELAQSGELQHLTAERIWLETEKALNEKNPEIYFETLHKTGALSVLFSEIDALYGVPNPVKHHPEVDSFIHTMLVLKQAV
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYFQELAELTCEFHTHIHKAFEL
RAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFETKDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTK
RRILAVKQTKTNYPTN

Sequences:

>Translated_416_residues
MKVYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYALARTERKSSAGYTGFICDFSP
TITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRILRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALM
AELAQSGELQHLTAERIWLETEKALNEKNPEIYFETLHKTGALSVLFSEIDALYGVPNPVKHHPEVDSFIHTMLVLKQAV
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYFQELAELTCEFHTHIHKAFEL
RAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFETKDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTK
RRILAVKQTKTNYPTN
>Mature_416_residues
MKVYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYALARTERKSSAGYTGFICDFSP
TITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRILRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALM
AELAQSGELQHLTAERIWLETEKALNEKNPEIYFETLHKTGALSVLFSEIDALYGVPNPVKHHPEVDSFIHTMLVLKQAV
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYFQELAELTCEFHTHIHKAFEL
RAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFETKDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTK
RRILAVKQTKTNYPTN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=192, Percent_Identity=32.2916666666667, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789436, Length=410, Percent_Identity=58.2926829268293, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI87081691, Length=180, Percent_Identity=34.4444444444444, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=238, Percent_Identity=28.1512605042017, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281360112, Length=370, Percent_Identity=25.1351351351351, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357337, Length=370, Percent_Identity=25.1351351351351, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47248; Mature: 47248

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSSAGYTGFICDFSPTITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRI
HHHHHHCCCCCEEEEECCCCCEEEHHHHHHHCCEEEEEECCCCCCEECCCCCHHHHHHHH
LRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALMAELAQSGELQHLTAERIWLE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TEKALNEKNPEIYFETLHKTGALSVLFSEIDALYGVPNPVKHHPEVDSFIHTMLVLKQAV
HHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
QELAELTCEFHTHIHKAFELRAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
KDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTKRRILAVKQTKTNYPTN
CCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKVYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSSAGYTGFICDFSPTITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRI
HHHHHHCCCCCEEEEECCCCCEEEHHHHHHHCCEEEEEECCCCCCEECCCCCHHHHHHHH
LRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALMAELAQSGELQHLTAERIWLE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TEKALNEKNPEIYFETLHKTGALSVLFSEIDALYGVPNPVKHHPEVDSFIHTMLVLKQAV
HHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
QELAELTCEFHTHIHKAFELRAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
KDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTKRRILAVKQTKTNYPTN
CCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA