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The map label for this gene is pykA

Identifier: 16273468

GI number: 16273468

Start: 1640232

End: 1641668

Strand: Direct

Name: pykA

Synonym: HI1573

Alternate gene names: 16273468

Gene position: 1640232-1641668 (Clockwise)

Preceding gene: 30995457

Following gene: 16273469

Centisome position: 89.62

GC content: 40.22

Gene sequence:

>1437_bases
ATGTCTAGAAGACTAAGAAGAACGAAGATTGTATGTACTATGGGCCCATCAACTGACCGTGATAACAATCTTGAAAAAAT
TATCGCAGCGGGCGCAAACGTAGTTCGTATGAACTTCTCTCACGGTACACCTGATGACCATATCGGACGTGCTGAACGTG
TACGTTCTATTGCGAAAAAATTAGGTAAAACCGTGGCAATCTTAGGTGATTTACAAGGTCCTAAAATTCGTGTTTCTACT
TTTAAAGACGGTAAAATTTTCTTAAACGTTGGCGATAAATTCATTCTTGATGCAGAGTTACCAAAAGGCGAAGGCACTCA
AGAATCCGTTGGTTTAGACTATAAAACGCTTCCTCAAGATGTTGTGCCGGGCGATATTCTTTTATTAGATGATGGCCGTG
TTCAATTAAAAGTATTATCAACTGATGGTGCAAAAGTTTTCACTGAAGTTACTGTTGGTGGTCCATTATCAAATAATAAA
GGTATCAATAAATTAGGTGGCGGTTTATCTGCGGATGCCCTAACAGAAAAAGATAAAGCCGACATTATTACCGCTGCACG
CATTGGTGTTGATTTCTTAGCCGTTTCTTTCCCTCGTTCAAGTGCAGATTTAAATTATGCACGTGAACTTGCTCAACAAG
CAGGTTTAAATGCAAAAATCGTTGCTAAAGTTGAACGTGCAGAAACCGTTGCTAATGATGAAGCCATGGACGATATTATT
TTAGCCTCTGATGTAATTATGGTTGCACGTGGTGACTTAGGTGTAGAAATCGGCGATCCTGAATTAGTCGGTGTACAGAA
AAAATTAATCCGTCGTTCACGTCAATTAAATCGTGCTGTAATTACAGCGACTCAAATGATGGAATCAATGATTAGTAACC
CAATGCCAACTCGTGCTGAAGTAATGGACGTTGCAAACGCAGTATTAGATGGAACTGATGCAGTTATGCTTTCTGCAGAA
ACAGCAGCTGGTCAATATCCTTCTGAAACAGTGGCAGCAATGGCTAGCGTATGTTTAGGTGCAGAAAAAATGCCAAGCAT
TAACGTTTCTCGTCACCGTATGGATAAAGAATTTGAAACTATTGAAGAATCTGTTGCGATGTCTGCAATGTATGCAGCAA
ACCACATGAAAGGTGTAGCGGCAATCGTCACTTTAAGTAGCACAGGCCGTACTCCATTATTAATGTCACGCATTAGCTCT
GGCTTACCAATCTTTGCTTTATCTCGTAATCAAGAAACCCTAAACCTTTGTGCACTATACCGCGGTGTAACACCAATTTA
TCACGGCGAAGAAAGTCGTACAGAAGCTGGTGCAAAAGCAGCACCTCAATCATTAAAAGAAAAAGGTTATTTATCTACTG
GCGATTTAGTGCTGGTAACCCAAGGTGGTCAAGGTGCGACACAAACTAACGTATGTCGTACATTAATTGTTGAATAA

Upstream 100 bases:

>100_bases
AAGTTTTAAGCGTATAATGTGCACCGATTTTTTATCTGGCTAATATGTAGCGAAGTAAAATAACACCTTTAAAATTTTGT
AATTATTGACGGAGTAAATA

Downstream 100 bases:

>100_bases
TCAACAATCTAAATATGTTAAAATAAAAGAGCGGTGGATTTTTCCACCGTTTTTTATTTCCTTTTTTCACCGCACTTTTC
ACTTAAATCCAATAGATATT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKKLGKTVAILGDLQGPKIRVST
FKDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNK
GINKLGGGLSADALTEKDKADIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDII
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFETIEESVAMSAMYAANHMKGVAAIVTLSSTGRTPLLMSRISS
GLPIFALSRNQETLNLCALYRGVTPIYHGEESRTEAGAKAAPQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE

Sequences:

>Translated_478_residues
MSRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKKLGKTVAILGDLQGPKIRVST
FKDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNK
GINKLGGGLSADALTEKDKADIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDII
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFETIEESVAMSAMYAANHMKGVAAIVTLSSTGRTPLLMSRISS
GLPIFALSRNQETLNLCALYRGVTPIYHGEESRTEAGAKAAPQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE
>Mature_477_residues
SRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKKLGKTVAILGDLQGPKIRVSTF
KDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNKG
INKLGGGLSADALTEKDKADIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDIIL
ASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFETIEESVAMSAMYAANHMKGVAAIVTLSSTGRTPLLMSRISSG
LPIFALSRNQETLNLCALYRGVTPIYHGEESRTEAGAKAAPQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family

Homologues:

Organism=Homo sapiens, GI33286422, Length=498, Percent_Identity=35.3413654618474, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI33286420, Length=498, Percent_Identity=35.3413654618474, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI33286418, Length=497, Percent_Identity=34.8088531187123, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI32967597, Length=501, Percent_Identity=33.9321357285429, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI10835121, Length=501, Percent_Identity=33.9321357285429, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=34.6020761245675, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=34.6020761245675, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=32.3404255319149, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=32.3404255319149, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=31.7757009345794, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=77.0833333333333, Blast_Score=753, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=38.0851063829787, Blast_Score=291, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=499, Percent_Identity=35.4709418837675, Blast_Score=274, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI71984413, Length=492, Percent_Identity=35.3658536585366, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17506829, Length=492, Percent_Identity=35.3658536585366, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17506831, Length=492, Percent_Identity=35.3658536585366, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI71984406, Length=492, Percent_Identity=35.3658536585366, Blast_Score=234, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6319279, Length=482, Percent_Identity=36.9294605809129, Blast_Score=256, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=485, Percent_Identity=34.020618556701, Blast_Score=242, Evalue=8e-65,
Organism=Drosophila melanogaster, GI28571814, Length=506, Percent_Identity=36.5612648221344, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24648964, Length=506, Percent_Identity=36.5612648221344, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=37.1007371007371, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=31.4285714285714, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24646914, Length=285, Percent_Identity=34.0350877192982, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KPYK_HAEIN (P43924)

Other databases:

- EMBL:   L42023
- PIR:   C64130
- RefSeq:   NP_439719.1
- ProteinModelPortal:   P43924
- GeneID:   950433
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1573
- NMPDR:   fig|71421.1.peg.1495
- TIGR:   HI_1573
- HOGENOM:   HBG734376
- OMA:   SRSRQLN
- ProtClustDB:   PRK05826
- BioCyc:   HINF71421:HI_1573-MONOMER
- BRENDA:   2.7.1.40
- GO:   GO:0006096
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00224 PK; PF02887 PK_C; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 51055; Mature: 50924

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 36-36 BINDING 251-251 BINDING 252-252 BINDING 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKK
CCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHH
LGKTVAILGDLQGPKIRVSTFKDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCHHHCCCCHHHCCCC
VVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNKGINKLGGGLSADALTEKDKA
CCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCCCCHHHCCCCHH
DIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDII
HEEEHHHHCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCHHHHHHH
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAE
HHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFET
HHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IEESVAMSAMYAANHMKGVAAIVTLSSTGRTPLLMSRISSGLPIFALSRNQETLNLCALY
HHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHH
RGVTPIYHGEESRTEAGAKAAPQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE
HCCCCEECCCCHHHHCCCHHCHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SRRLRRTKIVCTMGPSTDRDNNLEKIIAAGANVVRMNFSHGTPDDHIGRAERVRSIAKK
CCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHH
LGKTVAILGDLQGPKIRVSTFKDGKIFLNVGDKFILDAELPKGEGTQESVGLDYKTLPQD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCHHHCCCCHHHCCCC
VVPGDILLLDDGRVQLKVLSTDGAKVFTEVTVGGPLSNNKGINKLGGGLSADALTEKDKA
CCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCCCCHHHCCCCHH
DIITAARIGVDFLAVSFPRSSADLNYARELAQQAGLNAKIVAKVERAETVANDEAMDDII
HEEEHHHHCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCHHHHHHH
LASDVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRAVITATQMMESMISNPMPTRAE
HHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMASVCLGAEKMPSINVSRHRMDKEFET
HHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IEESVAMSAMYAANHMKGVAAIVTLSSTGRTPLLMSRISSGLPIFALSRNQETLNLCALY
HHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHH
RGVTPIYHGEESRTEAGAKAAPQSLKEKGYLSTGDLVLVTQGGQGATQTNVCRTLIVE
HCCCCEECCCCHHHHCCCHHCHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800