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The map label for this gene is hemK

Identifier: 16273459

GI number: 16273459

Start: 1629584

End: 1630462

Strand: Reverse

Name: hemK

Synonym: HI1559

Alternate gene names: 16273459

Gene position: 1630462-1629584 (Counterclockwise)

Preceding gene: 16273460

Following gene: 16273458

Centisome position: 89.09

GC content: 39.93

Gene sequence:

>879_bases
ATGAATTATAAAGAATGGCTGGCACAAGCGATTGCGGATTTAGCAAAAAAAAATCCCACTGAAAATAGTAAAATTGATGC
ACTTGTGCTTCTTCAACACGCTACTGGCAAATCGCGTACGCAAATCCTTGCTTTTGATGATACTGAGATTGATGAAAAAG
TGCGGTTAAAATTGACCGCACTTTTAGATCGTCGTCTAAAAGGTGAACCTATCGCTTATATTCTTGGTGAAAAAGAATTT
TGGTCGTTGCCATTAAATGTCTCAAAAGGCACATTGATTCCAAGACCAGATACTGAAATTTTAGTGGAAAAGGCATTACA
AATTGCGTTAGAAAAACTGGAAGAAAATCCACCGCACTTTCGTATTCTTGATCTCGGCACTGGCACAGGCGCAATCGCCC
TCGCTTTGGCTTCCGAGCTTGCGCCCATTTGCCAAAAGCGGCATATTCCGCTGGAAATAATTGGTGTGGATCTTATGTCC
GATGTTGTGGCATTAGCTCAATCTAATGCAGAAAGAAATCAGCTAAATGTAGAATTTTTGCAAAGCCGTTGGTTTGATAA
TATAACAGGGAAGTTTGATCTTATCGTCAGCAATCCGCCTTATATTGATGCGCAAGATGAACATTTGCATCAAGGCGATG
TGCGTTTTGAACCCCTTTCTGCATTAGTTGCCAATGATGAGGGGTATGCTGATTTACGTCATATTATTGAATTAGCATCG
AGTTATTTGAATTCTAATGGCGTATTATTGCTTGAACACGGATGGCAACAAGGAGAAAAAGTGCGGTCAATTTTCCAAGA
AAATCATTGGGAAATGGTTGAAACTGTGCGTGATTATAGCGACAATGAGCGTGTCACTTTAGGTTTTTGGAAGAAGTAA

Upstream 100 bases:

>100_bases
TCTTATTGATTATTAGCGGTATTGTTGCACTTGTACGCGATGATCGCCGTTCTTTAACTGATTTAGTGGCTGGCACGATA
GTGTTAAAAGACGAAAAATA

Downstream 100 bases:

>100_bases
TGAAATATTATCGACGTGCGTTATACGATAAATTTGTGCATTTTTATCTTGTTATCTCTGATGATGGCAGCTCAGAAGCA
CAACTTCGGGGAAAAATGGG

Product: hypothetical protein

Products: NA

Alternate protein names: M.HindHemKP

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNYKEWLAQAIADLAKKNPTENSKIDALVLLQHATGKSRTQILAFDDTEIDEKVRLKLTALLDRRLKGEPIAYILGEKEF
WSLPLNVSKGTLIPRPDTEILVEKALQIALEKLEENPPHFRILDLGTGTGAIALALASELAPICQKRHIPLEIIGVDLMS
DVVALAQSNAERNQLNVEFLQSRWFDNITGKFDLIVSNPPYIDAQDEHLHQGDVRFEPLSALVANDEGYADLRHIIELAS
SYLNSNGVLLLEHGWQQGEKVRSIFQENHWEMVETVRDYSDNERVTLGFWKK

Sequences:

>Translated_292_residues
MNYKEWLAQAIADLAKKNPTENSKIDALVLLQHATGKSRTQILAFDDTEIDEKVRLKLTALLDRRLKGEPIAYILGEKEF
WSLPLNVSKGTLIPRPDTEILVEKALQIALEKLEENPPHFRILDLGTGTGAIALALASELAPICQKRHIPLEIIGVDLMS
DVVALAQSNAERNQLNVEFLQSRWFDNITGKFDLIVSNPPYIDAQDEHLHQGDVRFEPLSALVANDEGYADLRHIIELAS
SYLNSNGVLLLEHGWQQGEKVRSIFQENHWEMVETVRDYSDNERVTLGFWKK
>Mature_292_residues
MNYKEWLAQAIADLAKKNPTENSKIDALVLLQHATGKSRTQILAFDDTEIDEKVRLKLTALLDRRLKGEPIAYILGEKEF
WSLPLNVSKGTLIPRPDTEILVEKALQIALEKLEENPPHFRILDLGTGTGAIALALASELAPICQKRHIPLEIIGVDLMS
DVVALAQSNAERNQLNVEFLQSRWFDNITGKFDLIVSNPPYIDAQDEHLHQGDVRFEPLSALVANDEGYADLRHIIELAS
SYLNSNGVLLLEHGWQQGEKVRSIFQENHWEMVETVRDYSDNERVTLGFWKK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family

Homologues:

Organism=Homo sapiens, GI7705409, Length=238, Percent_Identity=34.0336134453782, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1787463, Length=294, Percent_Identity=56.8027210884354, Blast_Score=298, Evalue=2e-82,
Organism=Escherichia coli, GI87082085, Length=189, Percent_Identity=32.8042328042328, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324266, Length=183, Percent_Identity=30.6010928961749, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582226, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_HAEIN (P45253)

Other databases:

- EMBL:   L42023
- PIR:   H64129
- RefSeq:   NP_439708.1
- ProteinModelPortal:   P45253
- SMR:   P45253
- GeneID:   950419
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1559
- NMPDR:   fig|71421.1.peg.1486
- TIGR:   HI_1559
- HOGENOM:   HBG732041
- OMA:   DPRPETE
- ProtClustDB:   PRK09328
- BioCyc:   HINF71421:HI_1559-MONOMER
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF05175 MTS

EC number: 2.1.1.-

Molecular weight: Translated: 33055; Mature: 33055

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 157-157 BINDING 184-184 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKEWLAQAIADLAKKNPTENSKIDALVLLQHATGKSRTQILAFDDTEIDEKVRLKLTA
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHH
LLDRRLKGEPIAYILGEKEFWSLPLNVSKGTLIPRPDTEILVEKALQIALEKLEENPPHF
HHHHHCCCCCEEEEECCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
RILDLGTGTGAIALALASELAPICQKRHIPLEIIGVDLMSDVVALAQSNAERNQLNVEFL
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHH
QSRWFDNITGKFDLIVSNPPYIDAQDEHLHQGDVRFEPLSALVANDEGYADLRHIIELAS
HHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEECHHHHEEECCCCHHHHHHHHHHHH
SYLNSNGVLLLEHGWQQGEKVRSIFQENHWEMVETVRDYSDNERVTLGFWKK
HHHCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECCC
>Mature Secondary Structure
MNYKEWLAQAIADLAKKNPTENSKIDALVLLQHATGKSRTQILAFDDTEIDEKVRLKLTA
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHH
LLDRRLKGEPIAYILGEKEFWSLPLNVSKGTLIPRPDTEILVEKALQIALEKLEENPPHF
HHHHHCCCCCEEEEECCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
RILDLGTGTGAIALALASELAPICQKRHIPLEIIGVDLMSDVVALAQSNAERNQLNVEFL
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHH
QSRWFDNITGKFDLIVSNPPYIDAQDEHLHQGDVRFEPLSALVANDEGYADLRHIIELAS
HHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEECHHHHEEECCCCHHHHHHHHHHHH
SYLNSNGVLLLEHGWQQGEKVRSIFQENHWEMVETVRDYSDNERVTLGFWKK
HHHCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800