Click here to switch to the map view.

The map label for this gene is bioD

Identifier: 16273352

GI number: 16273352

Start: 1532814

End: 1533542

Strand: Reverse

Name: bioD

Synonym: HI1445

Alternate gene names: 16273352

Gene position: 1533542-1532814 (Counterclockwise)

Preceding gene: 16273353

Following gene: 16273350

Centisome position: 83.79

GC content: 35.94

Gene sequence:

>729_bases
ATGAGTAGCTTTTTTGTCGCAGGGACAGATACGAATGTAGGGAAAACAACAGCAGCGCGCGCAATGATTCAAGCCTTGCA
AGGGCAGGGAGTGCAAGTTGTCGGCTATAAACCTATTGCTTGTTGTGGAGAAGAGAGTATTTATCCTGTCGCGCAATCAG
AAAATACATCTGATTATGATCATTTCGTAAATGCTGATGTATTAACGCTGATGCATTCAACAAAAGAAAATGTGTCTTAT
CAGGATATTAATAGTTATACTTTTAGCCACTGTGCACCCATGCTCACACAAGATAAAATGCGGATTAAATTAGATAAAAT
TAATTGTGATTTAACGCGTTTGAATCAAACTTATCAATCTGTTGTGGTGGAAGGATCTTTTGGTTTAATGACACCAATGG
CAGAAGGAAAAAGTTTTGCTGATTGGATTGCTCAACAAAAAATGCCAGTAGTACTTGTAGTTGGAATTAAAGATGGTTGC
GTAAATCACGCTTTATTGACGGTAAAGGTTATCCAACAGTTAGGTGTGCCGTTATTAGGTTGGATAGCAAATCGAATTAA
TCCATTATTAAGCCATTATGCAGAAATTGTTGATTTACTTGTTGAACATATTGATGCGCCGCTTTTAGGTAAAATTCCTT
ATTTACATAAACCCGAAGAACAAGAATTAGGGCATTATTTAACGAATATAGATCGATTAATGTATATGCAAACAGAGTTC
ATAAAATAA

Upstream 100 bases:

>100_bases
TGTCTATTTATAAGATAAAAAAAGTGAAAATTTGCGTTATCTCAAATTATTAAATAAAAAATTCCTTTAAAATCCACCGC
ACTTTTTAAAGGAGAAAAAA

Downstream 100 bases:

>100_bases
TAAAGTGCGGTTGAATTTTTTTAGTTTTGTATAAGGGCGAAATATCGCCCTTATTTTGTGTTTGAAAAGAAATATATTAT
TGTGATAATTAAGGTCTTAC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase 1; DTBS 1; Dethiobiotin synthase 1

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSSFFVAGTDTNVGKTTAARAMIQALQGQGVQVVGYKPIACCGEESIYPVAQSENTSDYDHFVNADVLTLMHSTKENVSY
QDINSYTFSHCAPMLTQDKMRIKLDKINCDLTRLNQTYQSVVVEGSFGLMTPMAEGKSFADWIAQQKMPVVLVVGIKDGC
VNHALLTVKVIQQLGVPLLGWIANRINPLLSHYAEIVDLLVEHIDAPLLGKIPYLHKPEEQELGHYLTNIDRLMYMQTEF
IK

Sequences:

>Translated_242_residues
MSSFFVAGTDTNVGKTTAARAMIQALQGQGVQVVGYKPIACCGEESIYPVAQSENTSDYDHFVNADVLTLMHSTKENVSY
QDINSYTFSHCAPMLTQDKMRIKLDKINCDLTRLNQTYQSVVVEGSFGLMTPMAEGKSFADWIAQQKMPVVLVVGIKDGC
VNHALLTVKVIQQLGVPLLGWIANRINPLLSHYAEIVDLLVEHIDAPLLGKIPYLHKPEEQELGHYLTNIDRLMYMQTEF
IK
>Mature_241_residues
SSFFVAGTDTNVGKTTAARAMIQALQGQGVQVVGYKPIACCGEESIYPVAQSENTSDYDHFVNADVLTLMHSTKENVSYQ
DINSYTFSHCAPMLTQDKMRIKLDKINCDLTRLNQTYQSVVVEGSFGLMTPMAEGKSFADWIAQQKMPVVLVVGIKDGCV
NHALLTVKVIQQLGVPLLGWIANRINPLLSHYAEIVDLLVEHIDAPLLGKIPYLHKPEEQELGHYLTNIDRLMYMQTEFI
K

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI87081944, Length=234, Percent_Identity=39.3162393162393, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1786995, Length=228, Percent_Identity=38.5964912280702, Blast_Score=167, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD1_HAEIN (P45209)

Other databases:

- EMBL:   L42023
- PIR:   I64123
- RefSeq:   NP_439597.1
- ProteinModelPortal:   P45209
- GeneID:   950720
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1445
- NMPDR:   fig|71421.1.peg.1379
- TIGR:   HI_1445
- HOGENOM:   HBG650065
- OMA:   INPGLSH
- ProtClustDB:   PRK00090
- BioCyc:   HINF71421:HI_1445-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 26967; Mature: 26836

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFFVAGTDTNVGKTTAARAMIQALQGQGVQVVGYKPIACCGEESIYPVAQSENTSDYD
CCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEECCCCCCCCCCCCCCCCCHH
HFVNADVLTLMHSTKENVSYQDINSYTFSHCAPMLTQDKMRIKLDKINCDLTRLNQTYQS
HHHCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
VVVEGSFGLMTPMAEGKSFADWIAQQKMPVVLVVGIKDGCVNHALLTVKVIQQLGVPLLG
HEEECCCCCCCCHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHH
WIANRINPLLSHYAEIVDLLVEHIDAPLLGKIPYLHKPEEQELGHYLTNIDRLMYMQTEF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IK
CC
>Mature Secondary Structure 
SSFFVAGTDTNVGKTTAARAMIQALQGQGVQVVGYKPIACCGEESIYPVAQSENTSDYD
CCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEECCCCCCCCCCCCCCCCCHH
HFVNADVLTLMHSTKENVSYQDINSYTFSHCAPMLTQDKMRIKLDKINCDLTRLNQTYQS
HHHCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
VVVEGSFGLMTPMAEGKSFADWIAQQKMPVVLVVGIKDGCVNHALLTVKVIQQLGVPLLG
HEEECCCCCCCCHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHH
WIANRINPLLSHYAEIVDLLVEHIDAPLLGKIPYLHKPEEQELGHYLTNIDRLMYMQTEF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800