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The map label for this gene is sbcB

Identifier: 16273288

GI number: 16273288

Start: 1472239

End: 1473660

Strand: Direct

Name: sbcB

Synonym: HI1377

Alternate gene names: 16273288

Gene position: 1472239-1473660 (Clockwise)

Preceding gene: 16273287

Following gene: 16273649

Centisome position: 80.44

GC content: 35.4

Gene sequence:

>1422_bases
ATGGTAAAAGATTTCAGCTTTTTTATTTACGATTATGAAAGTTTTGGTGTAAATCCAGCAACAGATCGCCCTGCGCAATT
TGCGGGTATTCGTACAGATGCTGATTTTAATATCATTGGCGAACCAATAATGTTTTATTGTAAACAAACCAACGATTATT
TACCGGCTCCTGAAGCCGTAATGGTAACAGGAATTACACCACAAGAATGTAATGAAAAGGGGCTTTCAGAGCCTGAATTC
GCCGCAAATATCTTAGCAGAATTTTCCCAACCCAATACTTGTGTCATGGGTTATAACAATATTCGCTACGATGATGAAAT
GACGCGTTATACGTTTTATCGTAATTTCATTGAACCTTATGAATACAGCTGGAAAAACGGAAATTCACGCTGGGATTTGT
TAGATTTAGTACGGGCCTGTTATGCCTTGCGTCCAGAAGGAATTAATTGGGCTTATGACGATGATGGAATGCCAAGTTTC
CGCTTAGAAAAACTCACTAAAGCAAATAGTATTGAGCATGAAAATGCTCACGATGCCATGGCGGATGTATATGCAACTAT
TGCTATGGCGAAATTAATTAAAGAAAAACAGCCTAAATTATTCCAATATTTCTTTGAAAATCGCGGTAAAAAAGAAATCG
AAAAATTAGTTGATACAGGCGCAATGACACCTTTAGTGCATGTTTCTGGAATGCTAGGGAATTATCGAGGAAATTGCACT
TGGGTTGCACCTTTAGCTTGGCATCCGACAAACCAAAACGCATTAATTGTGTGTGATTTAACTGGTGATATCGATAATTT
ATTAGCCAAAAGTGCGGATGAATTACGAGCAGATTTATATACAAAAAAATTGGAATTAGAAGAAAGGGGAGTTTCATCCG
TTCCGTTAAAACTTGTGCATATCAATAAATGTCCTATTTTAGCACCAGCTAAAACTTTGTTACCAGAAACAGCAAATCGA
TTGGGTATTGATCGTCAATTATGCTTGGATAATCTTGCAAAATTACGAGCATCTTTTGATATTCGTGAAAAAGTGGCGGA
TATTTTTAATGAAGAACGTCAATTTGCATCGAATGATAATGTAGAAACTGAGTTATACAATGGCTTTTTTAGCAATGCTG
ATAAAAATAATATGGCTATTTTACGTAGTTTACCTGCAGAAAAATTATCTGAACATGGTTTAGCTTTTGAAGATAAACGT
ATCCTTGAATTATTGTTCCATTATCGCGCTCGTCATTTTTATAAAACCCTCACACGCGCTGAACAAATTAAATGGAAAAA
ATACCGACAGAATAAACTTGAAAAAAGTGCGGTAGAGTTTGAAGCAAGTTTACAACGTTTAGTAGAGGYACATTCAGATA
ATTCTGAAAAACTATCTTTGCTACAACAAGTATATGAATATGGTATAAAATTACTAGGTTAG

Upstream 100 bases:

>100_bases
AAGAATTAGCAAGCTAAATTGGCTAGGTATTATTTTCAGTTTGTCGGCTATTTTCTGCCTATATTATTTAGATAAAATTA
TCGCGTAAAAGAGAGTAGTC

Downstream 100 bases:

>100_bases
TCGATACGTTGTCACATTTTGTCAAAAAGCACGTTTTAATAAAAACGTGCTTTTTTTGTTGTTTTTCTGGGAAGAAAATA
TCCAAGAAGTTTTCCAGATT

Product: exonuclease I

Products: NA

Alternate protein names: Exonuclease I; DNA deoxyribophosphodiesterase; dRPase

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MVKDFSFFIYDYESFGVNPATDRPAQFAGIRTDADFNIIGEPIMFYCKQTNDYLPAPEAVMVTGITPQECNEKGLSEPEF
AANILAEFSQPNTCVMGYNNIRYDDEMTRYTFYRNFIEPYEYSWKNGNSRWDLLDLVRACYALRPEGINWAYDDDGMPSF
RLEKLTKANSIEHENAHDAMADVYATIAMAKLIKEKQPKLFQYFFENRGKKEIEKLVDTGAMTPLVHVSGMLGNYRGNCT
WVAPLAWHPTNQNALIVCDLTGDIDNLLAKSADELRADLYTKKLELEERGVSSVPLKLVHINKCPILAPAKTLLPETANR
LGIDRQLCLDNLAKLRASFDIREKVADIFNEERQFASNDNVETELYNGFFSNADKNNMAILRSLPAEKLSEHGLAFEDKR
ILELLFHYRARHFYKTLTRAEQIKWKKYRQNKLEKSAVEFEASLQRLVEXHSDNSEKLSLLQQVYEYGIKLLG

Sequences:

>Translated_473_residues
MVKDFSFFIYDYESFGVNPATDRPAQFAGIRTDADFNIIGEPIMFYCKQTNDYLPAPEAVMVTGITPQECNEKGLSEPEF
AANILAEFSQPNTCVMGYNNIRYDDEMTRYTFYRNFIEPYEYSWKNGNSRWDLLDLVRACYALRPEGINWAYDDDGMPSF
RLEKLTKANSIEHENAHDAMADVYATIAMAKLIKEKQPKLFQYFFENRGKKEIEKLVDTGAMTPLVHVSGMLGNYRGNCT
WVAPLAWHPTNQNALIVCDLTGDIDNLLAKSADELRADLYTKKLELEERGVSSVPLKLVHINKCPILAPAKTLLPETANR
LGIDRQLCLDNLAKLRASFDIREKVADIFNEERQFASNDNVETELYNGFFSNADKNNMAILRSLPAEKLSEHGLAFEDKR
ILELLFHYRARHFYKTLTRAEQIKWKKYRQNKLEKSAVEFEASLQRLVEXHSDNSEKLSLLQQVYEYGIKLLG
>Mature_473_residues
MVKDFSFFIYDYESFGVNPATDRPAQFAGIRTDADFNIIGEPIMFYCKQTNDYLPAPEAVMVTGITPQECNEKGLSEPEF
AANILAEFSQPNTCVMGYNNIRYDDEMTRYTFYRNFIEPYEYSWKNGNSRWDLLDLVRACYALRPEGINWAYDDDGMPSF
RLEKLTKANSIEHENAHDAMADVYATIAMAKLIKEKQPKLFQYFFENRGKKEIEKLVDTGAMTPLVHVSGMLGNYRGNCT
WVAPLAWHPTNQNALIVCDLTGDIDNLLAKSADELRADLYTKKLELEERGVSSVPLKLVHINKCPILAPAKTLLPETANR
LGIDRQLCLDNLAKLRASFDIREKVADIFNEERQFASNDNVETELYNGFFSNADKNNMAILRSLPAEKLSEHGLAFEDKR
ILELLFHYRARHFYKTLTRAEQIKWKKYRQNKLEKSAVEFEASLQRLVEXHSDNSEKLSLLQQVYEYGIKLLG

Specific function: Also functions as a DNA deoxyribophosphodiesterase that release deoxyribose-phosphate moieties following the cleavage DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease AP lyase

COG id: COG2925

COG function: function code L; Exonuclease I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788321, Length=468, Percent_Identity=56.8376068376068, Blast_Score=562, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EX1_HAEIN (P45188)

Other databases:

- EMBL:   L42023
- PIR:   E64120
- RefSeq:   NP_439529.1
- ProteinModelPortal:   P45188
- GeneID:   950828
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1377
- NMPDR:   fig|71421.1.peg.1314
- TIGR:   HI_1377
- HOGENOM:   HBG366231
- OMA:   PYAREWQ
- ProtClustDB:   PRK11779
- BioCyc:   HINF71421:HI_1377-MONOMER
- BRENDA:   3.1.11.1
- InterPro:   IPR013620
- InterPro:   IPR013520
- InterPro:   IPR012337

Pfam domain/function: PF00929 Exonuc_X-T; PF08411 Exonuc_X-T_C; SSF53098 RNaseH_fold

EC number: =3.1.11.1

Molecular weight: Translated: 54334; Mature: 54334

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKDFSFFIYDYESFGVNPATDRPAQFAGIRTDADFNIIGEPIMFYCKQTNDYLPAPEAV
CCCCEEEEEEEHHHCCCCCCCCCCCHHCCEECCCCCCCCCCHHHHHHHCCCCCCCCCCEE
MVTGITPQECNEKGLSEPEFAANILAEFSQPNTCVMGYNNIRYDDEMTRYTFYRNFIEPY
EEECCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCC
EYSWKNGNSRWDLLDLVRACYALRPEGINWAYDDDGMPSFRLEKLTKANSIEHENAHDAM
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
ADVYATIAMAKLIKEKQPKLFQYFFENRGKKEIEKLVDTGAMTPLVHVSGMLGNYRGNCT
HHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEE
WVAPLAWHPTNQNALIVCDLTGDIDNLLAKSADELRADLYTKKLELEERGVSSVPLKLVH
EEEEEEECCCCCCEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
INKCPILAPAKTLLPETANRLGIDRQLCLDNLAKLRASFDIREKVADIFNEERQFASNDN
ECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VETELYNGFFSNADKNNMAILRSLPAEKLSEHGLAFEDKRILELLFHYRARHFYKTLTRA
CCHHHHHHHHCCCCCCCHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQIKWKKYRQNKLEKSAVEFEASLQRLVEXHSDNSEKLSLLQQVYEYGIKLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MVKDFSFFIYDYESFGVNPATDRPAQFAGIRTDADFNIIGEPIMFYCKQTNDYLPAPEAV
CCCCEEEEEEEHHHCCCCCCCCCCCHHCCEECCCCCCCCCCHHHHHHHCCCCCCCCCCEE
MVTGITPQECNEKGLSEPEFAANILAEFSQPNTCVMGYNNIRYDDEMTRYTFYRNFIEPY
EEECCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCC
EYSWKNGNSRWDLLDLVRACYALRPEGINWAYDDDGMPSFRLEKLTKANSIEHENAHDAM
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
ADVYATIAMAKLIKEKQPKLFQYFFENRGKKEIEKLVDTGAMTPLVHVSGMLGNYRGNCT
HHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEE
WVAPLAWHPTNQNALIVCDLTGDIDNLLAKSADELRADLYTKKLELEERGVSSVPLKLVH
EEEEEEECCCCCCEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
INKCPILAPAKTLLPETANRLGIDRQLCLDNLAKLRASFDIREKVADIFNEERQFASNDN
ECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VETELYNGFFSNADKNNMAILRSLPAEKLSEHGLAFEDKRILELLFHYRARHFYKTLTRA
CCHHHHHHHHCCCCCCCHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQIKWKKYRQNKLEKSAVEFEASLQRLVEXHSDNSEKLSLLQQVYEYGIKLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800