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The map label for this gene is pheT [H]

Identifier: 16273224

GI number: 16273224

Start: 1389323

End: 1391710

Strand: Direct

Name: pheT [H]

Synonym: HI1312

Alternate gene names: 16273224

Gene position: 1389323-1391710 (Clockwise)

Preceding gene: 16273223

Following gene: 16273225

Centisome position: 75.91

GC content: 40.66

Gene sequence:

>2388_bases
ATGAAATTTAGTGAACAGTGGGTAAGAGAATGGGTGAACCCTGCGGTGTCCACCGAGCAATTATGCGAGCAAATTACCAT
GCTTGGCTTAGAAGTAGATGGCGTGGAAGCCGTTGCGGGTACATTTAATGGTGTCGTTGTTGGTGAAGTGGTGGAATGTG
CACAACATCCAGATGCAGATAAATTACGTGTAACTAAAGTGAACGTAGGCGGTGATCGTTTATTGGATATCGTGTGCGGT
GCACCAAATTGTCGTCAAGGCTTAAAAGTTGCTTGCGCAACAGAAGGTGCGGTATTACCTGGCGATTTCAAAATTAAGAA
AACGAAATTACGTGGTCAGCCATCAGAGGGGATGCTTTGTTCATTTTCTGAATTAGGTATTGATGTTGAAGCTGATGGTA
TTATTGAATTGCCACTTGATGCACCAATTGGTACTGATTTACGTGAATATTTAGCTTTAGATGATAATGCCATCGAAATT
AGCTTAACGCCAAACCGTGCAGATTGTTTGAGCATTGCAGGTATTGCGCGTGAAATTGGTGTGGTAAATAAACAACTCGT
AAATCAACTGCACTTTGAAGCAGCGCCAGCAACAATTTCTGATAAAGTTCAAATTGATTTACAAGCACCAGAAGCTTGCC
CGCGTTATTTATTACGTGTGATAAAGAACGTCAATGTAAAAGCACCATCGCCAATGTGGATGCAAGAAAAATTGCGTCGT
TGTGGTATTCGTTCTATCGATCCTATTGTCGATATTACTAACTATATTTTGCTTGAGTTCGGTCAGCCAATGCACGCGTT
TGATGCGGCTAAAGTTACACAACCTGTTCAAGTTCGCTTCGCGAAAGAGGGGGAAGAATTAGTATTATTAGATGGCTCAA
CCGCAAAACTTCAATCAAATACGTTATTAATTGCTGACCAAAATGGGCCTTTAGCAATGGCAGGGATCTTTGGTGGTGCA
GCCAGTGGCGTGAATAGTGAAACGAAAGATGTGATTTTAGAATCCGCATTTTTCGCTCCATTAGCGATTGCAGGCCGTGC
AAGACAATATGGTTTACATACGGATGCATCGCACCGTTTTGAACGTGGTGTGGATTTTGAATTAGCGCGTAAAGCAATGG
AACGAGCAACGGCATTATTACTTGAAATCTGTGGTGGTGAAGCAGGTGAGATTTGTGAAGCAAGCAGTGAAACTCATCTT
CCTAAAGTCAATACAGTTCAACTTCGCCGTAGTAAATTAGATGCACTTTTAGGTCATCACATTGAAACAGGAAGCGTAAC
GGAAATTTTCCACCGTCTTGGTTTTGATGTTACTTATGCAAATGATATTTGGACGGTAACTTCTGCAAGCTGGCGTTTTG
ATATTGAAATCGAAGAAGATTTAATTGAAGAAGTGGCGCGTATTTATGGTTATAACAGCATTCCAAACAATGCGCCATTA
GCGCATCTCTGCATGCGTGAGCACAAAGAATCTGATTTAGATTTAGCTCGAATTAAGACCGCACTTGTGGATGCAGATTA
TCAAGAAGCTATTACTTATAGCTTTGTGGATCCAAAAATTCAAAGTTTATTACATCCACATCAAGAAGCACTTGTATTGC
CAAACCCAATTTCTGTGGAAATGTCCGCAATGCGCGTGTCTTTAATCAGCGGTTTATTAGGTGCAGTGCTTTATAACCAA
AATCGCCAACAATCCCGTGTTCGTTTATTTGAAACAGGATTACGTTTTGTGCCAGATGCCAATGCTGAATTTGGCGTGCG
TCAAGAATTTGTTTTAAGTGCGGTGATTACTGGAACGGCAAAATCTGAACATTGGGCAGGTAAAGCAGAGTCTGTAGATT
TCTTTGATCTTAAAGGCGATTTAGAATCAGTACTTTCTTTAACAGAAGGTGGTCATAGAGTTCGTTTTGTTGCAAAACAA
TTTGATGCATTACACCCTGGCCAATCTGCTGCTATTGAATTAGATGGTCAAGAAATTGGTTTTATTGGTGCAATTCATCC
ATCTATTAGCCAAAAACTTGGCTTAAATGGCAAAACTTTTGTATTTGAAATTCTTTGGAATGCAATTGCGGCACGTAATG
TGGTGCAAGCAAAAGAAATTTCTAAATTCCCAGCAAACCGTCGTGATTTAGCTTTAGTCGTCGCGGATAGCGTACCAGCA
GGGGAATTAATCGCAGCATGTAAACAAGCAGGTGGCGAAAAATTGGTGCAAGTGAACTTGTTCGATGTATATCAAGGTGT
GGGCGTCGCTGAAGGCTATAAGAGTTTAGCGATTAGCTTGACTGTCCAAGATAATGAAAAAACACTTGAAGATGAAGAAA
TTAATGCAGTGATTTCAGCAGTATTAGCGGAGGTAAAACAACGCTTTAATGCTGAATTAAGGGATTAA

Upstream 100 bases:

>100_bases
TTATAACGTAACAGATTTACGTTCGTTCTTTGAAAACGACTTACGTTTCTTAAAACAATTTAAGTAATTTCTCGGATTTG
AATTAAAAGGATAACAACAA

Downstream 100 bases:

>100_bases
TATGGCTACGATAACTAAACTTGATATTATTGAATATTTAAGCGATAAATATCACTTATCTAAACAAGATACGAAAAATG
TAGTGGAGAACTTTTTGGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSEQWVREWVNPAVSTEQLCEQITMLGLEVDGVEAVAGTFNGVVVGEVVECAQHPDADKLRVTKVNVGGDRLLDIVCG
APNCRQGLKVACATEGAVLPGDFKIKKTKLRGQPSEGMLCSFSELGIDVEADGIIELPLDAPIGTDLREYLALDDNAIEI
SLTPNRADCLSIAGIAREIGVVNKQLVNQLHFEAAPATISDKVQIDLQAPEACPRYLLRVIKNVNVKAPSPMWMQEKLRR
CGIRSIDPIVDITNYILLEFGQPMHAFDAAKVTQPVQVRFAKEGEELVLLDGSTAKLQSNTLLIADQNGPLAMAGIFGGA
ASGVNSETKDVILESAFFAPLAIAGRARQYGLHTDASHRFERGVDFELARKAMERATALLLEICGGEAGEICEASSETHL
PKVNTVQLRRSKLDALLGHHIETGSVTEIFHRLGFDVTYANDIWTVTSASWRFDIEIEEDLIEEVARIYGYNSIPNNAPL
AHLCMREHKESDLDLARIKTALVDADYQEAITYSFVDPKIQSLLHPHQEALVLPNPISVEMSAMRVSLISGLLGAVLYNQ
NRQQSRVRLFETGLRFVPDANAEFGVRQEFVLSAVITGTAKSEHWAGKAESVDFFDLKGDLESVLSLTEGGHRVRFVAKQ
FDALHPGQSAAIELDGQEIGFIGAIHPSISQKLGLNGKTFVFEILWNAIAARNVVQAKEISKFPANRRDLALVVADSVPA
GELIAACKQAGGEKLVQVNLFDVYQGVGVAEGYKSLAISLTVQDNEKTLEDEEINAVISAVLAEVKQRFNAELRD

Sequences:

>Translated_795_residues
MKFSEQWVREWVNPAVSTEQLCEQITMLGLEVDGVEAVAGTFNGVVVGEVVECAQHPDADKLRVTKVNVGGDRLLDIVCG
APNCRQGLKVACATEGAVLPGDFKIKKTKLRGQPSEGMLCSFSELGIDVEADGIIELPLDAPIGTDLREYLALDDNAIEI
SLTPNRADCLSIAGIAREIGVVNKQLVNQLHFEAAPATISDKVQIDLQAPEACPRYLLRVIKNVNVKAPSPMWMQEKLRR
CGIRSIDPIVDITNYILLEFGQPMHAFDAAKVTQPVQVRFAKEGEELVLLDGSTAKLQSNTLLIADQNGPLAMAGIFGGA
ASGVNSETKDVILESAFFAPLAIAGRARQYGLHTDASHRFERGVDFELARKAMERATALLLEICGGEAGEICEASSETHL
PKVNTVQLRRSKLDALLGHHIETGSVTEIFHRLGFDVTYANDIWTVTSASWRFDIEIEEDLIEEVARIYGYNSIPNNAPL
AHLCMREHKESDLDLARIKTALVDADYQEAITYSFVDPKIQSLLHPHQEALVLPNPISVEMSAMRVSLISGLLGAVLYNQ
NRQQSRVRLFETGLRFVPDANAEFGVRQEFVLSAVITGTAKSEHWAGKAESVDFFDLKGDLESVLSLTEGGHRVRFVAKQ
FDALHPGQSAAIELDGQEIGFIGAIHPSISQKLGLNGKTFVFEILWNAIAARNVVQAKEISKFPANRRDLALVVADSVPA
GELIAACKQAGGEKLVQVNLFDVYQGVGVAEGYKSLAISLTVQDNEKTLEDEEINAVISAVLAEVKQRFNAELRD
>Mature_795_residues
MKFSEQWVREWVNPAVSTEQLCEQITMLGLEVDGVEAVAGTFNGVVVGEVVECAQHPDADKLRVTKVNVGGDRLLDIVCG
APNCRQGLKVACATEGAVLPGDFKIKKTKLRGQPSEGMLCSFSELGIDVEADGIIELPLDAPIGTDLREYLALDDNAIEI
SLTPNRADCLSIAGIAREIGVVNKQLVNQLHFEAAPATISDKVQIDLQAPEACPRYLLRVIKNVNVKAPSPMWMQEKLRR
CGIRSIDPIVDITNYILLEFGQPMHAFDAAKVTQPVQVRFAKEGEELVLLDGSTAKLQSNTLLIADQNGPLAMAGIFGGA
ASGVNSETKDVILESAFFAPLAIAGRARQYGLHTDASHRFERGVDFELARKAMERATALLLEICGGEAGEICEASSETHL
PKVNTVQLRRSKLDALLGHHIETGSVTEIFHRLGFDVTYANDIWTVTSASWRFDIEIEEDLIEEVARIYGYNSIPNNAPL
AHLCMREHKESDLDLARIKTALVDADYQEAITYSFVDPKIQSLLHPHQEALVLPNPISVEMSAMRVSLISGLLGAVLYNQ
NRQQSRVRLFETGLRFVPDANAEFGVRQEFVLSAVITGTAKSEHWAGKAESVDFFDLKGDLESVLSLTEGGHRVRFVAKQ
FDALHPGQSAAIELDGQEIGFIGAIHPSISQKLGLNGKTFVFEILWNAIAARNVVQAKEISKFPANRRDLALVVADSVPA
GELIAACKQAGGEKLVQVNLFDVYQGVGVAEGYKSLAISLTVQDNEKTLEDEEINAVISAVLAEVKQRFNAELRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=65.2830188679245, Blast_Score=1056, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=552, Percent_Identity=22.8260869565217, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86748; Mature: 86748

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSEQWVREWVNPAVSTEQLCEQITMLGLEVDGVEAVAGTFNGVVVGEVVECAQHPDAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCEECCHHHHHHCCCCEEHHHHHHHHCCCCCC
KLRVTKVNVGGDRLLDIVCGAPNCRQGLKVACATEGAVLPGDFKIKKTKLRGQPSEGMLC
CEEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCEE
SFSELGIDVEADGIIELPLDAPIGTDLREYLALDDNAIEISLTPNRADCLSIAGIAREIG
EHHHCCCEECCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHH
VVNKQLVNQLHFEAAPATISDKVQIDLQAPEACPRYLLRVIKNVNVKAPSPMWMQEKLRR
HHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDPIVDITNYILLEFGQPMHAFDAAKVTQPVQVRFAKEGEELVLLDGSTAKLQSN
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCEEECC
TLLIADQNGPLAMAGIFGGAASGVNSETKDVILESAFFAPLAIAGRARQYGLHTDASHRF
EEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHH
ERGVDFELARKAMERATALLLEICGGEAGEICEASSETHLPKVNTVQLRRSKLDALLGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
IETGSVTEIFHRLGFDVTYANDIWTVTSASWRFDIEIEEDLIEEVARIYGYNSIPNNAPL
CCCCCHHHHHHHHCCCEEECCCEEEEECCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCH
AHLCMREHKESDLDLARIKTALVDADYQEAITYSFVDPKIQSLLHPHQEALVLPNPISVE
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHCCCCCEEECCCCCCCH
MSAMRVSLISGLLGAVLYNQNRQQSRVRLFETGLRFVPDANAEFGVRQEFVLSAVITGTA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
KSEHWAGKAESVDFFDLKGDLESVLSLTEGGHRVRFVAKQFDALHPGQSAAIELDGQEIG
CCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCEEEECCCEEE
FIGAIHPSISQKLGLNGKTFVFEILWNAIAARNVVQAKEISKFPANRRDLALVVADSVPA
EEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCH
GELIAACKQAGGEKLVQVNLFDVYQGVGVAEGYKSLAISLTVQDNEKTLEDEEINAVISA
HHHHHHHHHCCCCEEEEEEHHHHHCCCCHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
VLAEVKQRFNAELRD
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MKFSEQWVREWVNPAVSTEQLCEQITMLGLEVDGVEAVAGTFNGVVVGEVVECAQHPDAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCEECCHHHHHHCCCCEEHHHHHHHHCCCCCC
KLRVTKVNVGGDRLLDIVCGAPNCRQGLKVACATEGAVLPGDFKIKKTKLRGQPSEGMLC
CEEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCEE
SFSELGIDVEADGIIELPLDAPIGTDLREYLALDDNAIEISLTPNRADCLSIAGIAREIG
EHHHCCCEECCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHH
VVNKQLVNQLHFEAAPATISDKVQIDLQAPEACPRYLLRVIKNVNVKAPSPMWMQEKLRR
HHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDPIVDITNYILLEFGQPMHAFDAAKVTQPVQVRFAKEGEELVLLDGSTAKLQSN
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCEEECC
TLLIADQNGPLAMAGIFGGAASGVNSETKDVILESAFFAPLAIAGRARQYGLHTDASHRF
EEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHH
ERGVDFELARKAMERATALLLEICGGEAGEICEASSETHLPKVNTVQLRRSKLDALLGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
IETGSVTEIFHRLGFDVTYANDIWTVTSASWRFDIEIEEDLIEEVARIYGYNSIPNNAPL
CCCCCHHHHHHHHCCCEEECCCEEEEECCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCH
AHLCMREHKESDLDLARIKTALVDADYQEAITYSFVDPKIQSLLHPHQEALVLPNPISVE
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHCCCCCEEECCCCCCCH
MSAMRVSLISGLLGAVLYNQNRQQSRVRLFETGLRFVPDANAEFGVRQEFVLSAVITGTA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
KSEHWAGKAESVDFFDLKGDLESVLSLTEGGHRVRFVAKQFDALHPGQSAAIELDGQEIG
CCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCEEEECCCEEE
FIGAIHPSISQKLGLNGKTFVFEILWNAIAARNVVQAKEISKFPANRRDLALVVADSVPA
EEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCH
GELIAACKQAGGEKLVQVNLFDVYQGVGVAEGYKSLAISLTVQDNEKTLEDEEINAVISA
HHHHHHHHHCCCCEEEEEEHHHHHCCCCHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
VLAEVKQRFNAELRD
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA