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The map label for this gene is pheS [H]

Identifier: 16273223

GI number: 16273223

Start: 1388300

End: 1389289

Strand: Direct

Name: pheS [H]

Synonym: HI1311

Alternate gene names: 16273223

Gene position: 1388300-1389289 (Clockwise)

Preceding gene: 16273222

Following gene: 16273224

Centisome position: 75.86

GC content: 38.99

Gene sequence:

>990_bases
ATGCAACATCTGAACGAGCTTGTTGAGAAAGCGAAATTAGCGATTGAATCCATTCAGGATAAAAGTTTAACGGCTTTGGA
TGAAATCCGTGTCGAATATTTTGGTAAGAAAGGGCATTTCACGCAATTAATGCAAGAGTTGCGTAACGTGTCAGCTGAAG
AACGTCCTGCGATGGGCGCAAAAATTAACGAAGCAAAACAAGCTGCGTTAGAATTTTTAAATGCAAAAAAAACGGAGTGG
GAACAGGCTGAACTTAACTCAAAATTAGAAAAAGAACGTGTAGATGTCAGTTTACCTGGTCGCAAAGTTGAAACCGGTGG
CTTACACCCAGTTACCATGACGATTAATCGCGTAACAAAATTTTTCTCAGAACTTGGCTTTTCAGTTGAAAATGGTCCTG
AAATTGAAAGTGATTATTACAACTTCGATGCGTTAAATATTCCTAAACATCACCCAGCACGTGCCGATCACGATACTTTC
TGGTTTAATCCAGAATTATTACTTCGTACTCAAACTTCTGGCGTGCAAATTCGTACTATGGAAAAAATGCAGCCACCAAT
TCGCATTATGGCTCCAGGACGTGTATATCGTAATGACTACGATCAAACGCATACGCCAATGTTCCATCAAATTGAACTAC
TTTATGTGGATAAAAAAGCAAATTTCACAGAATTAAAAGGCTTATTACACGATTTCTTACGCGCTTTCTTTGAAGAAGAT
TTACAAGTACGTTTCCGTCCATCTTATTTCCCATTCACAGAGCCTTCAGCGGAAGTGGATGTGATGGGGAAAAACGGTAA
ATGGTTAGAAGTGTTGGGCTGCGGTATGGTGCATCCAAATGTGTTGCGTAATGTTGGTATTGATCCGAATGAATATTCTG
GTTTTGCAGTGGGAATGGGCGTTGAGCGTTTAACAATGTTACGTTATAACGTAACAGATTTACGTTCGTTCTTTGAAAAC
GACTTACGTTTCTTAAAACAATTTAAGTAA

Upstream 100 bases:

>100_bases
AAGCCAATGATTTTTCGCATTATTCTTTTACTTTTTAAGGACTTGGTATTATCATTACTCGGTTTTTTATTTTATAGAGA
AAAGAGAAGCAGGGGAAACT

Downstream 100 bases:

>100_bases
TTTCTCGGATTTGAATTAAAAGGATAACAACAAATGAAATTTAGTGAACAGTGGGTAAGAGAATGGGTGAACCCTGCGGT
GTCCACCGAGCAATTATGCG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MQHLNELVEKAKLAIESIQDKSLTALDEIRVEYFGKKGHFTQLMQELRNVSAEERPAMGAKINEAKQAALEFLNAKKTEW
EQAELNSKLEKERVDVSLPGRKVETGGLHPVTMTINRVTKFFSELGFSVENGPEIESDYYNFDALNIPKHHPARADHDTF
WFNPELLLRTQTSGVQIRTMEKMQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEED
LQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYSGFAVGMGVERLTMLRYNVTDLRSFFEN
DLRFLKQFK

Sequences:

>Translated_329_residues
MQHLNELVEKAKLAIESIQDKSLTALDEIRVEYFGKKGHFTQLMQELRNVSAEERPAMGAKINEAKQAALEFLNAKKTEW
EQAELNSKLEKERVDVSLPGRKVETGGLHPVTMTINRVTKFFSELGFSVENGPEIESDYYNFDALNIPKHHPARADHDTF
WFNPELLLRTQTSGVQIRTMEKMQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEED
LQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYSGFAVGMGVERLTMLRYNVTDLRSFFEN
DLRFLKQFK
>Mature_329_residues
MQHLNELVEKAKLAIESIQDKSLTALDEIRVEYFGKKGHFTQLMQELRNVSAEERPAMGAKINEAKQAALEFLNAKKTEW
EQAELNSKLEKERVDVSLPGRKVETGGLHPVTMTINRVTKFFSELGFSVENGPEIESDYYNFDALNIPKHHPARADHDTF
WFNPELLLRTQTSGVQIRTMEKMQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEED
LQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYSGFAVGMGVERLTMLRYNVTDLRSFFEN
DLRFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=318, Percent_Identity=28.6163522012579, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4758340, Length=259, Percent_Identity=31.2741312741313, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788007, Length=329, Percent_Identity=74.7720364741641, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI32566635, Length=242, Percent_Identity=33.4710743801653, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=28.8461538461538, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=28.8461538461538, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=28.4644194756554, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=41.1111111111111, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI18858079, Length=260, Percent_Identity=29.6153846153846, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17137424, Length=280, Percent_Identity=28.2142857142857, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38081; Mature: 38081

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLNELVEKAKLAIESIQDKSLTALDEIRVEYFGKKGHFTQLMQELRNVSAEERPAMGA
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC
KINEAKQAALEFLNAKKTEWEQAELNSKLEKERVDVSLPGRKVETGGLHPVTMTINRVTK
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEECCCCEECCCCCCEEEHHHHHHHH
FFSELGFSVENGPEIESDYYNFDALNIPKHHPARADHDTFWFNPELLLRTQTSGVQIRTM
HHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEEH
EKMQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEED
HHCCCCEEEECCCHHHCCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCC
LQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYSGFAVGMG
CEEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEEECC
VERLTMLRYNVTDLRSFFENDLRFLKQFK
HHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQHLNELVEKAKLAIESIQDKSLTALDEIRVEYFGKKGHFTQLMQELRNVSAEERPAMGA
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC
KINEAKQAALEFLNAKKTEWEQAELNSKLEKERVDVSLPGRKVETGGLHPVTMTINRVTK
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEECCCCEECCCCCCEEEHHHHHHHH
FFSELGFSVENGPEIESDYYNFDALNIPKHHPARADHDTFWFNPELLLRTQTSGVQIRTM
HHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEEH
EKMQPPIRIMAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLLHDFLRAFFEED
HHCCCCEEEECCCHHHCCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCC
LQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPNEYSGFAVGMG
CEEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEEECC
VERLTMLRYNVTDLRSFFENDLRFLKQFK
HHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA