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The map label for this gene is nusA

Identifier: 16273197

GI number: 16273197

Start: 1359128

End: 1360615

Strand: Direct

Name: nusA

Synonym: HI1283

Alternate gene names: 16273197

Gene position: 1359128-1360615 (Clockwise)

Preceding gene: 30995436

Following gene: 16273198

Centisome position: 74.26

GC content: 38.98

Gene sequence:

>1488_bases
ATGAGTAAAGAAATTTTACTGGCGGCGGAAGCTGTATCCAATGAAAAATTATTACCACGTGAGAAAATTTTTGAAGCATT
AGAGAGTGCGATTGCGATTTCTACTAAGAAGAAGTTTGAATATGAAACGGATATTCGCGTTTCAATTAATCCCAAAACAG
GAGAATTTGATACTTTTCGCCGTTGGTTAGTCGTAGATGAAGTAAAAGTACCAACGAAGGAAATTACTTTGGAAGCGGCG
CAATTTGATGATCCAAATTTGCAACTTGGAGAGTATGTAGAAGATCAAATTGAATCTATTGCATTTGACCGTATTGCAAT
GCAAACGGCACGTCAAGTTATTAGCACTAAAATTCGTGAAGCCGAACGTGCAAAAGTGGTTGAACAATTTCGTGAAGAAG
AAGGAAAAATTGTAACAGGCACTGTAAAAAAAGTAAGTCGAGACAGTATTATTTTAGATCTTGGCAATAAAGCGGAAGCG
ATGATTGCGCGTGAAGATATGTTACCACGTGAAAACTTCCGTCCGGGAGATCGTGTTCGTGGGGTACTTTATAAAGTAAA
TCCTGAAGGCAAAACTGCACAATTATTTGTGACACGTTCTAAACCAGAAATGTTGATCGAATTATTCCGTATTGAAGTGC
CTGAAATTGGCGAAGAAATGATTGAAATTCGTGGTGCAGCTCGTGATGCTGGCTCTCGCGCTAAAATTGCAGTGAAATCA
AATGATAAGCGAATTGATCCTGTTGGTGCTTGTGTAGGAATGCGTGGTGCGCGTGTTCAGGTAATTACGAATGAACTTGG
TGGCGAGCGTGTAGATATCGTGCTTTGGGATGACAATCCCGCGCAATTTGTGATTAATGCGATGGCACCAGCGGATGTTT
CTTCTATTATCGTTGATGAAGATAATCATTCTATGGATATTGCTGTTGAAGCTGATAATCTTGCTCAAGCCATTGGTCGT
AATGGTCAAAACGTGCGTTTAGCAACACAATTAACGGGTTGGACACTAAACGTAATGACGACTGATGAGTTAAATGCTAA
GCATCAAGCAGAAGATAACAAAGTGTTAAATTTATTTATTAACGCATTAGAACTTGATGAAGAATTTGCTCAAATCCTTG
TGGAAGAAGGCTTCACAAGTTTAGAAGAAATTGCTTATGTGCCAATGAATGAATTGACAGCAATTGATGGTTTGGAAGAT
GAAGATCTTGTTGAAGAGCTTCAAACTCGTGCTAAAAATGCGATTACAGCCGCAGCAGTAGCTGAAGAAGAAGCACTGAA
AAAAGCGAATGTTGAAGATCGTTTATTGAATTTAGAGGGAATGAATCGTCACGTTGCAATCAAACTAGCAGAAAAACAAA
TTACCACCCTTGAAGAACTCGCAGAACAAGGCGTTGATGATTTAACTGATATTGAAGAATTAACCGCAGAACAGGCCGCA
GATTTAATTATGGCTGCACGTAATATTTGTTGGTTTGGCGAAGAATAA

Upstream 100 bases:

>100_bases
ATGATTACTTTAATTGTTGATGATCAAGAACAAATTTTAGTCTTCGGAAACATTCAAAAAGCGAATGTTGTTGCAAAATT
TTAAAAGGAGAAAAAGAGAA

Downstream 100 bases:

>100_bases
GGAGAGCAGTAATGACTGAAGATGTTAAGGCTGATGCGCCAAAAAAATTAAGCATTCAGCGCAGAACAAAAACAACAGTG
AGCAGCACCACAACTGGCGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFRRWLVVDEVKVPTKEITLEAA
QFDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIREAERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEA
MIAREDMLPRENFRPGDRVRGVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKS
NDKRIDPVGACVGMRGARVQVITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDEDNHSMDIAVEADNLAQAIGR
NGQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFINALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLED
EDLVEELQTRAKNAITAAAVAEEEALKKANVEDRLLNLEGMNRHVAIKLAEKQITTLEELAEQGVDDLTDIEELTAEQAA
DLIMAARNICWFGEE

Sequences:

>Translated_495_residues
MSKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFRRWLVVDEVKVPTKEITLEAA
QFDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIREAERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEA
MIAREDMLPRENFRPGDRVRGVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKS
NDKRIDPVGACVGMRGARVQVITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDEDNHSMDIAVEADNLAQAIGR
NGQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFINALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLED
EDLVEELQTRAKNAITAAAVAEEEALKKANVEDRLLNLEGMNRHVAIKLAEKQITTLEELAEQGVDDLTDIEELTAEQAA
DLIMAARNICWFGEE
>Mature_494_residues
SKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFRRWLVVDEVKVPTKEITLEAAQ
FDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIREAERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEAM
IAREDMLPRENFRPGDRVRGVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKSN
DKRIDPVGACVGMRGARVQVITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDEDNHSMDIAVEADNLAQAIGRN
GQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFINALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLEDE
DLVEELQTRAKNAITAAAVAEEEALKKANVEDRLLNLEGMNRHVAIKLAEKQITTLEELAEQGVDDLTDIEELTAEQAAD
LIMAARNICWFGEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI1789560, Length=495, Percent_Identity=73.1313131313131, Blast_Score=736, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSA_HAEIN (P43915)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_439435.1
- ProteinModelPortal:   P43915
- GeneID:   950214
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1283
- NMPDR:   fig|71421.1.peg.1223
- TIGR:   HI_1283
- HOGENOM:   HBG529269
- OMA:   KAREPWF
- ProtClustDB:   PRK09202
- BioCyc:   HINF71421:HI_1283-MONOMER
- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213
- Gene3D:   G3DSA:3.30.300.20
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.30.1480.10
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR01953
- TIGRFAMs:   TIGR01954

Pfam domain/function: PF08529 NusA_N; PF00575 S1; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF47794 Rad51_N; SSF69705 TF_NusA_N

EC number: NA

Molecular weight: Translated: 55263; Mature: 55132

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFR
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
RWLVVDEVKVPTKEITLEAAQFDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIRE
HEEEEECCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEAMIAREDMLPRENFRPGDRVR
HHHHHHHHHHHHHCCCEEEEEHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHC
GVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKS
EEEEEECCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEC
NDKRIDPVGACVGMRGARVQVITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDE
CCCCCCHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCHHEEEEECCCCCCCEEEEEC
DNHSMDIAVEADNLAQAIGRNGQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFI
CCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHH
NALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLEDEDLVEELQTRAKNAITAAAV
HHHHHHHHHHHHHHHHCCHHHHHHEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AEEEALKKANVEDRLLNLEGMNRHVAIKLAEKQITTLEELAEQGVDDLTDIEELTAEQAA
HHHHHHHHCCHHHHEECCCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DLIMAARNICWFGEE
HHHHHHHHCEECCCC
>Mature Secondary Structure 
SKEILLAAEAVSNEKLLPREKIFEALESAIAISTKKKFEYETDIRVSINPKTGEFDTFR
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
RWLVVDEVKVPTKEITLEAAQFDDPNLQLGEYVEDQIESIAFDRIAMQTARQVISTKIRE
HEEEEECCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAKVVEQFREEEGKIVTGTVKKVSRDSIILDLGNKAEAMIAREDMLPRENFRPGDRVR
HHHHHHHHHHHHHCCCEEEEEHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHC
GVLYKVNPEGKTAQLFVTRSKPEMLIELFRIEVPEIGEEMIEIRGAARDAGSRAKIAVKS
EEEEEECCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEC
NDKRIDPVGACVGMRGARVQVITNELGGERVDIVLWDDNPAQFVINAMAPADVSSIIVDE
CCCCCCHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCHHEEEEECCCCCCCEEEEEC
DNHSMDIAVEADNLAQAIGRNGQNVRLATQLTGWTLNVMTTDELNAKHQAEDNKVLNLFI
CCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHH
NALELDEEFAQILVEEGFTSLEEIAYVPMNELTAIDGLEDEDLVEELQTRAKNAITAAAV
HHHHHHHHHHHHHHHHCCHHHHHHEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AEEEALKKANVEDRLLNLEGMNRHVAIKLAEKQITTLEELAEQGVDDLTDIEELTAEQAA
HHHHHHHHCCHHHHEECCCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DLIMAARNICWFGEE
HHHHHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800