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The map label for this gene is folC

Identifier: 16273179

GI number: 16273179

Start: 1338150

End: 1339463

Strand: Direct

Name: folC

Synonym: HI1261

Alternate gene names: 16273179

Gene position: 1338150-1339463 (Clockwise)

Preceding gene: 16273178

Following gene: 16273181

Centisome position: 73.12

GC content: 40.26

Gene sequence:

>1314_bases
ATGAATAATATGCAACTTAAAGCCACTTCGCCACTCGCTGAGTGGCTTTCTTATTTAGAAAAAAGCCATTTTAAACCTAT
TGATCTCGGTTTAGATCGTATTAAATCCGTAGCGGAAAAATTGGATTTACTTCATCCCGTCCCTTATGTGATAACTGTGG
GGGGGACGAATGGTAAAGGCACGACTTGTCGCTTGTTAGAAACGATTCTGCTTAATCACGGATTACGTGTAGGCGTGTAT
TCTTCACCCCATTTATTGCGTTATAACGAGCGAGTACGCATTCAAAATCAAGATTTACCCGATGAAGCACATACCGCCTC
TTTTGCTTTTATTGATGAAAACAAAACAGAATCGCTGACTTATTTTGAATTTAGCACCCTTTCTGCCTTGCATTTATTTA
AGCAAGCAAAATTAGATGTGGTGATTTTAGAAGTGGGGTTGGGCGGTCGTTTAGATGCGACCAATATTGTGGATAGCCAT
CTTGCTGTGATTACTAGCATCGATATTGATCACACCGATTTTCTTGGGGATACCAGAGAGGCCATTGCTTTTGAAAAAGC
AGGAATTTTCCGCGAAAATTGCCCTGTCGTGATTGGTGAACCTAATGTGCCACAAACAATGTTAGATCAGGCTGAAAAAT
TACATTGCCAAGTTGCTCGTCGTGATGTGGATTGGTTATTTGAGCAAAATGCTGAAAATTGGCAATGGCAAAATAAAAAA
GTGCGGTTAGAAAATTTGCCGTTTTGTCAGATTCCATTGGCGAATGCCGCAACGGTTCTTGCTGCTGTTCAATATTTACC
TTTTGATATTTCAGAACAGACTTTGCGAAAATCTTTGCAAGAGGTAGAACTGGTTGGTCGTTTTCAAGCTATAAAAACAG
ATAAACGTGAGAAATTAGCGGATTATCTTGGCGTACCAGTTGAAACCTTGCCAACAATCATCGTTGATGTGGGGCATAAT
CCTCACGCTGCAAAATATTTATCTGAAAAATTGACCGCACTTAAACGTAGCATCGAGGGCAAAATGATTGCCGTGTGTGG
GATGCTCAAAGACAAAGATGCAAATGGGGTATTTGAGCATTTAACACCTATTATTGATGAATGGCATTGTGTAACTTTAG
GCGGTTATCGCGGGCAGTCAGGCGATGAATTAGTAGAGAAATTGAAAAGCCATTTCCCAAATATTCAAGCTACATCAGAT
AATTCTGTCATGGATGGTGTGTGTACAGCACTCAAAAGTGCGGTAAAAAATGATGTGGTTTTAGTCTTTGGATCTTTCCA
CACCGTTGCTGAATTTTGGGCTGTGGTGGAATAA

Upstream 100 bases:

>100_bases
ATCGTGAAACGTTCAGAAATGCGTCAAACTTTAGCAAGTGTACTGAGTAAATTAACGAATCAACCTTCTCCTTTTGTAGA
ACCTGAACTCATTTCAGAAG

Downstream 100 bases:

>100_bases
AATTTATCATTAAAACAAAGGGCTGATATTTAAAATCAGCCCTTATTTTTTGGAATATTATCTCCAAGAATATTTCACAC
CCACGGTGCCTTGGTAGCCT

Product: folylpolyglutamate synthase/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNNMQLKATSPLAEWLSYLEKSHFKPIDLGLDRIKSVAEKLDLLHPVPYVITVGGTNGKGTTCRLLETILLNHGLRVGVY
SSPHLLRYNERVRIQNQDLPDEAHTASFAFIDENKTESLTYFEFSTLSALHLFKQAKLDVVILEVGLGGRLDATNIVDSH
LAVITSIDIDHTDFLGDTREAIAFEKAGIFRENCPVVIGEPNVPQTMLDQAEKLHCQVARRDVDWLFEQNAENWQWQNKK
VRLENLPFCQIPLANAATVLAAVQYLPFDISEQTLRKSLQEVELVGRFQAIKTDKREKLADYLGVPVETLPTIIVDVGHN
PHAAKYLSEKLTALKRSIEGKMIAVCGMLKDKDANGVFEHLTPIIDEWHCVTLGGYRGQSGDELVEKLKSHFPNIQATSD
NSVMDGVCTALKSAVKNDVVLVFGSFHTVAEFWAVVE

Sequences:

>Translated_437_residues
MNNMQLKATSPLAEWLSYLEKSHFKPIDLGLDRIKSVAEKLDLLHPVPYVITVGGTNGKGTTCRLLETILLNHGLRVGVY
SSPHLLRYNERVRIQNQDLPDEAHTASFAFIDENKTESLTYFEFSTLSALHLFKQAKLDVVILEVGLGGRLDATNIVDSH
LAVITSIDIDHTDFLGDTREAIAFEKAGIFRENCPVVIGEPNVPQTMLDQAEKLHCQVARRDVDWLFEQNAENWQWQNKK
VRLENLPFCQIPLANAATVLAAVQYLPFDISEQTLRKSLQEVELVGRFQAIKTDKREKLADYLGVPVETLPTIIVDVGHN
PHAAKYLSEKLTALKRSIEGKMIAVCGMLKDKDANGVFEHLTPIIDEWHCVTLGGYRGQSGDELVEKLKSHFPNIQATSD
NSVMDGVCTALKSAVKNDVVLVFGSFHTVAEFWAVVE
>Mature_437_residues
MNNMQLKATSPLAEWLSYLEKSHFKPIDLGLDRIKSVAEKLDLLHPVPYVITVGGTNGKGTTCRLLETILLNHGLRVGVY
SSPHLLRYNERVRIQNQDLPDEAHTASFAFIDENKTESLTYFEFSTLSALHLFKQAKLDVVILEVGLGGRLDATNIVDSH
LAVITSIDIDHTDFLGDTREAIAFEKAGIFRENCPVVIGEPNVPQTMLDQAEKLHCQVARRDVDWLFEQNAENWQWQNKK
VRLENLPFCQIPLANAATVLAAVQYLPFDISEQTLRKSLQEVELVGRFQAIKTDKREKLADYLGVPVETLPTIIVDVGHN
PHAAKYLSEKLTALKRSIEGKMIAVCGMLKDKDANGVFEHLTPIIDEWHCVTLGGYRGQSGDELVEKLKSHFPNIQATSD
NSVMDGVCTALKSAVKNDVVLVFGSFHTVAEFWAVVE

Specific function: Conversion of folates to polyglutamate derivatives

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family

Homologues:

Organism=Homo sapiens, GI66932984, Length=207, Percent_Identity=39.1304347826087, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI66932990, Length=207, Percent_Identity=39.1304347826087, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788654, Length=432, Percent_Identity=49.537037037037, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71984923, Length=171, Percent_Identity=38.0116959064327, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=171, Percent_Identity=38.0116959064327, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17553148, Length=171, Percent_Identity=38.0116959064327, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=323, Percent_Identity=30.030959752322, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324815, Length=178, Percent_Identity=34.8314606741573, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24641571, Length=198, Percent_Identity=37.8787878787879, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24581568, Length=186, Percent_Identity=36.0215053763441, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLC_HAEIN (P43775)

Other databases:

- EMBL:   L42023
- PIR:   C64113
- RefSeq:   NP_439416.1
- ProteinModelPortal:   P43775
- SMR:   P43775
- GeneID:   950205
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1261
- NMPDR:   fig|71421.1.peg.1206
- TIGR:   HI_1261
- HOGENOM:   HBG744947
- OMA:   KIENIAT
- ProtClustDB:   CLSK870180
- BioCyc:   HINF71421:HI_1261-MONOMER
- BRENDA:   6.3.2.17
- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- PANTHER:   PTHR11136
- TIGRFAMs:   TIGR01499

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.17

Molecular weight: Translated: 48826; Mature: 48826

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMQLKATSPLAEWLSYLEKSHFKPIDLGLDRIKSVAEKLDLLHPVPYVITVGGTNGKG
CCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
TTCRLLETILLNHGLRVGVYSSPHLLRYNERVRIQNQDLPDEAHTASFAFIDENKTESLT
HHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEEECCCCCCCHHCCEEEEEECCCCCCEE
YFEFSTLSALHLFKQAKLDVVILEVGLGGRLDATNIVDSHLAVITSIDIDHTDFLGDTRE
EEEHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHEEEEEEECCCCHHHCCCHHH
AIAFEKAGIFRENCPVVIGEPNVPQTMLDQAEKLHCQVARRDVDWLFEQNAENWQWQNKK
HHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCE
VRLENLPFCQIPLANAATVLAAVQYLPFDISEQTLRKSLQEVELVGRFQAIKTDKREKLA
EEECCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
DYLGVPVETLPTIIVDVGHNPHAAKYLSEKLTALKRSIEGKMIAVCGMLKDKDANGVFEH
HHHCCCHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
LTPIIDEWHCVTLGGYRGQSGDELVEKLKSHFPNIQATSDNSVMDGVCTALKSAVKNDVV
HHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCEE
LVFGSFHTVAEFWAVVE
EEEECHHHHHHHHHHCC
>Mature Secondary Structure
MNNMQLKATSPLAEWLSYLEKSHFKPIDLGLDRIKSVAEKLDLLHPVPYVITVGGTNGKG
CCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
TTCRLLETILLNHGLRVGVYSSPHLLRYNERVRIQNQDLPDEAHTASFAFIDENKTESLT
HHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEEECCCCCCCHHCCEEEEEECCCCCCEE
YFEFSTLSALHLFKQAKLDVVILEVGLGGRLDATNIVDSHLAVITSIDIDHTDFLGDTRE
EEEHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHEEEEEEECCCCHHHCCCHHH
AIAFEKAGIFRENCPVVIGEPNVPQTMLDQAEKLHCQVARRDVDWLFEQNAENWQWQNKK
HHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCE
VRLENLPFCQIPLANAATVLAAVQYLPFDISEQTLRKSLQEVELVGRFQAIKTDKREKLA
EEECCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
DYLGVPVETLPTIIVDVGHNPHAAKYLSEKLTALKRSIEGKMIAVCGMLKDKDANGVFEH
HHHCCCHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
LTPIIDEWHCVTLGGYRGQSGDELVEKLKSHFPNIQATSDNSVMDGVCTALKSAVKNDVV
HHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCEE
LVFGSFHTVAEFWAVVE
EEEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800