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The map label for this gene is recJ

Identifier: 16273133

GI number: 16273133

Start: 1281398

End: 1283125

Strand: Direct

Name: recJ

Synonym: HI1214

Alternate gene names: 16273133

Gene position: 1281398-1283125 (Clockwise)

Preceding gene: 16273132

Following gene: 30995432

Centisome position: 70.02

GC content: 40.34

Gene sequence:

>1728_bases
GTGAAAAAACTTATCAAACGCCGTGAAATTCCAATTGGAAATTCTGTTTCAAACCATCCCTTATTAGATCGACTTTATCG
CGCTCGTCATATTCAAAACACCAAAGAATTAGACCGCACTTTGAAATCAATGCTCAATCCAAATCAGTTATATGGCATTG
AGCAAGCGGTAAATTTATTAGTGGAAGCCTATCAACCACAGCAAAAAATTGTGATTGTTGGGGATTTTGATGCTGACGGT
GCAACCAGCACAGCATTGAGTGTGCTGGCTTTAAGACAACTTGGCTTTTCTGATGTGGATTACCTTGTGCCAAATCGATT
TGAACAAGGTTATGGTTTGAGTATTCCCGTGGCTGAAATGGCAATTGAAAAGGGCGTTCAGCTTTTAATGACGGTGGATA
ACGGCGTTTCATCTTTTGACGGTGTGGCATTTTTGAAAGAAAAAGGCATTCGAGTTTTAGTTACGGATCATCATTTACCG
CCCGAAACTTTGCCGCCAGCCGATGCGATTGTTAATCCTAATTTAAGTCAGTGTGGTTTTCCTTCAAAATCCTTAGCGGG
CGTTGGTGTGGCGTTTTATTTGATGTTAGCCGTACGAGCAAAATTTCGTGAACTCGGTATTTTTACGGCAGAAACACAAC
CTAATTTTACTGATTTGCTCGATCTGGTGGCACTAGGCACTATAGCTGATGTTGTTCCTTTGGATCAAAATAATCGTATC
CTTGCTTATCAAGGTTTAATGCGTATCCGTGCTAGACACTGCCGTCTTGGTATTATTGCGTTGGCAGAAGTGGCTAACCG
TAATGTAGAGCAGTTTACTTCAAGTGATCTCGGTTTTTGTATTGGACCGCGTTTAAATGCGGCAGGGCGTTTAGATAATA
TGTCGATTGGGGTAGAACTTTTGCTGGCGAACGAAATGTCAAAAGCCAGAGAATTAGCTTTAGATCTCGATCAGCTTAAC
CAAACTCGTAAAGAAATTGAAGCAGGTATGAAGCTTGAGGCAATAAAAATTTGTCAAAATCTTACCGCACTTTTTAAAGA
GTTACCCTATGGTATTACGCTTTATCAACCAGATTGGCATCAGGGCGTATTAGGCATTGTGTCATCAAGGATTAAGGATC
AATATCATCGTCCAGTGATTGCCTTTGCACAAGATAGTGAGGGAATTTTGAAAGGTTCCGCGCGTTCTATTGAAGGTTTA
CATATGCGTGATGTTCTGGAGCGTATTCATTCGCAACATCCTAATATGATTTTAAAATTTGGCGGACACGCAATGGCGGC
AGGCTTGAGTATTCGCGAAGAACATTTTGCGGATTTCCAACATATTTTTAATCAAACCGTTGCCGATTGGCTTGATGAAG
AACATCTACAAGGCGTAATTTGGACAGATGGCGAACTGAATTCAAATGAATTTAATCTTGAAACGGCAGAGCTGATAAAA
TCGGTAGGCACTTGGGGGCAAGGGTTTCCTGAGCCTTGCTTTGATGGCGAATTTAAAATTTTAGACCAGCGCGCAATTGG
GCAAAATAAAAATCATCTAAAAATGTTGCTAGAGCCGAAACAAGGCGGGGTTTTATTAGATGCGATTGCATTTAATATTA
ATACAAGATTGTATCCTGATCTTTCAATTAAACAGGCTCGTTTAGCCTATAAATTAGAGATTAACGAATTTCGTGGCAAT
CGTAGCCTACAATTATTGGTAGATTATATTGAGCCGATAGATGAGTGA

Upstream 100 bases:

>100_bases
CAGCATTCAGGGTTAAAAGCCCTGACCAACAGGAAAAAGTATAGATAAAAGAGCTGTTGATTTCAACAGCTTTTTGCTTT
TAGGTATGAATTAATATCCA

Downstream 100 bases:

>100_bases
TAGAATGAAATTAAAAGGGTTATTGGCATTTTGTTTGCTTTTTTTATCGTCGTTTGTTTTAGCAGAAGTCAATCAGAAAG
AATTTTCAGTGCAAAATTCT

Product: single-stranded-DNA-specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MKKLIKRREIPIGNSVSNHPLLDRLYRARHIQNTKELDRTLKSMLNPNQLYGIEQAVNLLVEAYQPQQKIVIVGDFDADG
ATSTALSVLALRQLGFSDVDYLVPNRFEQGYGLSIPVAEMAIEKGVQLLMTVDNGVSSFDGVAFLKEKGIRVLVTDHHLP
PETLPPADAIVNPNLSQCGFPSKSLAGVGVAFYLMLAVRAKFRELGIFTAETQPNFTDLLDLVALGTIADVVPLDQNNRI
LAYQGLMRIRARHCRLGIIALAEVANRNVEQFTSSDLGFCIGPRLNAAGRLDNMSIGVELLLANEMSKARELALDLDQLN
QTRKEIEAGMKLEAIKICQNLTALFKELPYGITLYQPDWHQGVLGIVSSRIKDQYHRPVIAFAQDSEGILKGSARSIEGL
HMRDVLERIHSQHPNMILKFGGHAMAAGLSIREEHFADFQHIFNQTVADWLDEEHLQGVIWTDGELNSNEFNLETAELIK
SVGTWGQGFPEPCFDGEFKILDQRAIGQNKNHLKMLLEPKQGGVLLDAIAFNINTRLYPDLSIKQARLAYKLEINEFRGN
RSLQLLVDYIEPIDE

Sequences:

>Translated_575_residues
MKKLIKRREIPIGNSVSNHPLLDRLYRARHIQNTKELDRTLKSMLNPNQLYGIEQAVNLLVEAYQPQQKIVIVGDFDADG
ATSTALSVLALRQLGFSDVDYLVPNRFEQGYGLSIPVAEMAIEKGVQLLMTVDNGVSSFDGVAFLKEKGIRVLVTDHHLP
PETLPPADAIVNPNLSQCGFPSKSLAGVGVAFYLMLAVRAKFRELGIFTAETQPNFTDLLDLVALGTIADVVPLDQNNRI
LAYQGLMRIRARHCRLGIIALAEVANRNVEQFTSSDLGFCIGPRLNAAGRLDNMSIGVELLLANEMSKARELALDLDQLN
QTRKEIEAGMKLEAIKICQNLTALFKELPYGITLYQPDWHQGVLGIVSSRIKDQYHRPVIAFAQDSEGILKGSARSIEGL
HMRDVLERIHSQHPNMILKFGGHAMAAGLSIREEHFADFQHIFNQTVADWLDEEHLQGVIWTDGELNSNEFNLETAELIK
SVGTWGQGFPEPCFDGEFKILDQRAIGQNKNHLKMLLEPKQGGVLLDAIAFNINTRLYPDLSIKQARLAYKLEINEFRGN
RSLQLLVDYIEPIDE
>Mature_575_residues
MKKLIKRREIPIGNSVSNHPLLDRLYRARHIQNTKELDRTLKSMLNPNQLYGIEQAVNLLVEAYQPQQKIVIVGDFDADG
ATSTALSVLALRQLGFSDVDYLVPNRFEQGYGLSIPVAEMAIEKGVQLLMTVDNGVSSFDGVAFLKEKGIRVLVTDHHLP
PETLPPADAIVNPNLSQCGFPSKSLAGVGVAFYLMLAVRAKFRELGIFTAETQPNFTDLLDLVALGTIADVVPLDQNNRI
LAYQGLMRIRARHCRLGIIALAEVANRNVEQFTSSDLGFCIGPRLNAAGRLDNMSIGVELLLANEMSKARELALDLDQLN
QTRKEIEAGMKLEAIKICQNLTALFKELPYGITLYQPDWHQGVLGIVSSRIKDQYHRPVIAFAQDSEGILKGSARSIEGL
HMRDVLERIHSQHPNMILKFGGHAMAAGLSIREEHFADFQHIFNQTVADWLDEEHLQGVIWTDGELNSNEFNLETAELIK
SVGTWGQGFPEPCFDGEFKILDQRAIGQNKNHLKMLLEPKQGGVLLDAIAFNINTRLYPDLSIKQARLAYKLEINEFRGN
RSLQLLVDYIEPIDE

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family

Homologues:

Organism=Escherichia coli, GI1789259, Length=573, Percent_Identity=57.7661431064572, Blast_Score=677, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECJ_HAEIN (P45112)

Other databases:

- EMBL:   L42023
- PIR:   F64110
- RefSeq:   NP_439370.1
- ProteinModelPortal:   P45112
- SMR:   P45112
- GeneID:   950889
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1214
- NMPDR:   fig|71421.1.peg.1160
- TIGR:   HI_1214
- HOGENOM:   HBG616253
- OMA:   HLPGHEL
- ProtClustDB:   CLSK892397
- BioCyc:   HINF71421:HI_1214-MONOMER
- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- TIGRFAMs:   TIGR00644

Pfam domain/function: PF01368 DHH; PF02272 DHHA1

EC number: 3.1.-.- [C]

Molecular weight: Translated: 64220; Mature: 64220

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIKRREIPIGNSVSNHPLLDRLYRARHIQNTKELDRTLKSMLNPNQLYGIEQAVNLL
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
VEAYQPQQKIVIVGDFDADGATSTALSVLALRQLGFSDVDYLVPNRFEQGYGLSIPVAEM
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHH
AIEKGVQLLMTVDNGVSSFDGVAFLKEKGIRVLVTDHHLPPETLPPADAIVNPNLSQCGF
HHHCCCEEEEEECCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCHHCCCCCHHHCCC
PSKSLAGVGVAFYLMLAVRAKFRELGIFTAETQPNFTDLLDLVALGTIADVVPLDQNNRI
CCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCE
LAYQGLMRIRARHCRLGIIALAEVANRNVEQFTSSDLGFCIGPRLNAAGRLDNMSIGVEL
EHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEE
LLANEMSKARELALDLDQLNQTRKEIEAGMKLEAIKICQNLTALFKELPYGITLYQPDWH
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHH
QGVLGIVSSRIKDQYHRPVIAFAQDSEGILKGSARSIEGLHMRDVLERIHSQHPNMILKF
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
GGHAMAAGLSIREEHFADFQHIFNQTVADWLDEEHLQGVIWTDGELNSNEFNLETAELIK
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEEECCCCCCCCCCCHHHHHHH
SVGTWGQGFPEPCFDGEFKILDQRAIGQNKNHLKMLLEPKQGGVLLDAIAFNINTRLYPD
HHCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCEEEEEECCCCCEEEEEEEEECCCEECCC
LSIKQARLAYKLEINEFRGNRSLQLLVDYIEPIDE
CCHHHHHEEEEEEEHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLIKRREIPIGNSVSNHPLLDRLYRARHIQNTKELDRTLKSMLNPNQLYGIEQAVNLL
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
VEAYQPQQKIVIVGDFDADGATSTALSVLALRQLGFSDVDYLVPNRFEQGYGLSIPVAEM
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHH
AIEKGVQLLMTVDNGVSSFDGVAFLKEKGIRVLVTDHHLPPETLPPADAIVNPNLSQCGF
HHHCCCEEEEEECCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCHHCCCCCHHHCCC
PSKSLAGVGVAFYLMLAVRAKFRELGIFTAETQPNFTDLLDLVALGTIADVVPLDQNNRI
CCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCE
LAYQGLMRIRARHCRLGIIALAEVANRNVEQFTSSDLGFCIGPRLNAAGRLDNMSIGVEL
EHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEE
LLANEMSKARELALDLDQLNQTRKEIEAGMKLEAIKICQNLTALFKELPYGITLYQPDWH
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHH
QGVLGIVSSRIKDQYHRPVIAFAQDSEGILKGSARSIEGLHMRDVLERIHSQHPNMILKF
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
GGHAMAAGLSIREEHFADFQHIFNQTVADWLDEEHLQGVIWTDGELNSNEFNLETAELIK
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEEECCCCCCCCCCCHHHHHHH
SVGTWGQGFPEPCFDGEFKILDQRAIGQNKNHLKMLLEPKQGGVLLDAIAFNINTRLYPD
HHCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCEEEEEECCCCCEEEEEEEEECCCEECCC
LSIKQARLAYKLEINEFRGNRSLQLLVDYIEPIDE
CCHHHHHEEEEEEEHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800