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The map label for this gene is mraW [H]

Identifier: 16273056

GI number: 16273056

Start: 1196536

End: 1197501

Strand: Direct

Name: mraW [H]

Synonym: HI1130

Alternate gene names: 16273056

Gene position: 1196536-1197501 (Clockwise)

Preceding gene: 16273055

Following gene: 16273057

Centisome position: 65.38

GC content: 37.78

Gene sequence:

>966_bases
ATGAATAGCGAAAATTCCTTTTCTTCATCTGAACATATTACAGTTTTACTTCACGAAGCCGTGAATGGCTTGGCATTGAA
GGAGAATGGCATTTATATTGATGGTACTTTTGGGCGTGGGGGGCATTCTCGGTTTATCCTTTCTCAACTTTCTTCTAATG
GTCGTTTGATAGCTGTAGATCGCGATCCTCGTGCTATTGCAGAAGCACACAAAATCCAAGACTTGCGTTTTCAGATTGAA
CATAACAGCTTTTCGCATATTCCTGAAATTTGTGACAAATTAAATTTAGTGGGCAAAATTGACGGTATTTTGCTTGATCT
TGGTGTGTCTTCCCCTCAGCTTGATGAAGCAGAACGTGGTTTTAGTTTTATGAAAGATGGCCCGCTTGATATGCGTATGG
ATACAACTCAAGGTTTATCTGCTGAAGAATGGTTAAAACAAGTGTCCATTGAGGATTTAACTTGGGTGTTGAAAACTTTT
GGCGAAGAGCGTTTCGCTAAACGTATTGCCACTGCTATTGTTGATTTCAATAAAAGTGCGGTAAAAAATGGCACAGAATT
TTTATCGCGTACCAGTCAATTGGCGGAACTTATTTCACAGGCAGTTCCTTTTAAAGATAAACATAAACATCCTGCGACGC
GTAGTTTCCAAGCTATTCGTATTTTTATTAATTCGGAATTAGATGAATTAGAAAGTCTGCTTAATTCTGCGTTAGATATG
TTAGCACCAGAAGGTCGTTTATCAATTATTAGTTTCCATTCTTTAGAAGATAGAATGGTGAAACATTTTATGAAAAAACA
AAGTAAGGGCGAGGATATTCCCAAAGGTTTACCATTGCGAGAAGATCAAATTCAGCGTAATCAAAAATTAAGAATTATTG
GTAAAGCCATTCAGCCAAGTGATGCAGAAATTCAAGCCAATCCTCGTTCAAGAAGTGCCATATTACGTGTGGCAGAGAGA
ATTTAG

Upstream 100 bases:

>100_bases
TGCAGAGGATATAGAAATTGGCTCCAGCACAGATTTTGCTGCTGATGCGTTAAATGATTTCTCATTATAGAAAATAATGT
AGTACTTTTATCTTAAATTT

Downstream 100 bases:

>100_bases
CGATGTCTGAAAATAATAAGCCTCGTTATCCGTTACAGCAGATTTTAGTCGAAGATTTATTTTCTTCAAATAAGTTAGTG
GTGTTGCTGTTAATAGGGAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNSENSFSSSEHITVLLHEAVNGLALKENGIYIDGTFGRGGHSRFILSQLSSNGRLIAVDRDPRAIAEAHKIQDLRFQIE
HNSFSHIPEICDKLNLVGKIDGILLDLGVSSPQLDEAERGFSFMKDGPLDMRMDTTQGLSAEEWLKQVSIEDLTWVLKTF
GEERFAKRIATAIVDFNKSAVKNGTEFLSRTSQLAELISQAVPFKDKHKHPATRSFQAIRIFINSELDELESLLNSALDM
LAPEGRLSIISFHSLEDRMVKHFMKKQSKGEDIPKGLPLREDQIQRNQKLRIIGKAIQPSDAEIQANPRSRSAILRVAER
I

Sequences:

>Translated_321_residues
MNSENSFSSSEHITVLLHEAVNGLALKENGIYIDGTFGRGGHSRFILSQLSSNGRLIAVDRDPRAIAEAHKIQDLRFQIE
HNSFSHIPEICDKLNLVGKIDGILLDLGVSSPQLDEAERGFSFMKDGPLDMRMDTTQGLSAEEWLKQVSIEDLTWVLKTF
GEERFAKRIATAIVDFNKSAVKNGTEFLSRTSQLAELISQAVPFKDKHKHPATRSFQAIRIFINSELDELESLLNSALDM
LAPEGRLSIISFHSLEDRMVKHFMKKQSKGEDIPKGLPLREDQIQRNQKLRIIGKAIQPSDAEIQANPRSRSAILRVAER
I
>Mature_321_residues
MNSENSFSSSEHITVLLHEAVNGLALKENGIYIDGTFGRGGHSRFILSQLSSNGRLIAVDRDPRAIAEAHKIQDLRFQIE
HNSFSHIPEICDKLNLVGKIDGILLDLGVSSPQLDEAERGFSFMKDGPLDMRMDTTQGLSAEEWLKQVSIEDLTWVLKTF
GEERFAKRIATAIVDFNKSAVKNGTEFLSRTSQLAELISQAVPFKDKHKHPATRSFQAIRIFINSELDELESLLNSALDM
LAPEGRLSIISFHSLEDRMVKHFMKKQSKGEDIPKGLPLREDQIQRNQKLRIIGKAIQPSDAEIQANPRSRSAILRVAER
I

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=345, Percent_Identity=37.9710144927536, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI165377202, Length=196, Percent_Identity=39.2857142857143, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=60.8974358974359, Blast_Score=393, Evalue=1e-111,
Organism=Drosophila melanogaster, GI62472493, Length=338, Percent_Identity=35.5029585798817, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI28571637, Length=338, Percent_Identity=35.5029585798817, Blast_Score=171, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 36053; Mature: 36053

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSENSFSSSEHITVLLHEAVNGLALKENGIYIDGTFGRGGHSRFILSQLSSNGRLIAVD
CCCCCCCCCCCCEEEEEEHHHCCEEEECCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEC
RDPRAIAEAHKIQDLRFQIEHNSFSHIPEICDKLNLVGKIDGILLDLGVSSPQLDEAERG
CCCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHC
FSFMKDGPLDMRMDTTQGLSAEEWLKQVSIEDLTWVLKTFGEERFAKRIATAIVDFNKSA
CHHHCCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VKNGTEFLSRTSQLAELISQAVPFKDKHKHPATRSFQAIRIFINSELDELESLLNSALDM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
LAPEGRLSIISFHSLEDRMVKHFMKKQSKGEDIPKGLPLREDQIQRNQKLRIIGKAIQPS
HCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCC
DAEIQANPRSRSAILRVAERI
CCEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNSENSFSSSEHITVLLHEAVNGLALKENGIYIDGTFGRGGHSRFILSQLSSNGRLIAVD
CCCCCCCCCCCCEEEEEEHHHCCEEEECCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEC
RDPRAIAEAHKIQDLRFQIEHNSFSHIPEICDKLNLVGKIDGILLDLGVSSPQLDEAERG
CCCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHC
FSFMKDGPLDMRMDTTQGLSAEEWLKQVSIEDLTWVLKTFGEERFAKRIATAIVDFNKSA
CHHHCCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VKNGTEFLSRTSQLAELISQAVPFKDKHKHPATRSFQAIRIFINSELDELESLLNSALDM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
LAPEGRLSIISFHSLEDRMVKHFMKKQSKGEDIPKGLPLREDQIQRNQKLRIIGKAIQPS
HCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCC
DAEIQANPRSRSAILRVAERI
CCEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA