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The map label for this gene is galE [C]

Identifier: 16272949

GI number: 16272949

Start: 1078046

End: 1078993

Strand: Direct

Name: galE [C]

Synonym: HI1014

Alternate gene names: 16272949

Gene position: 1078046-1078993 (Clockwise)

Preceding gene: 16272948

Following gene: 16272950

Centisome position: 58.91

GC content: 35.55

Gene sequence:

>948_bases
ATGAAAGTTGTAATTACTGGTGGACAAGGGTTCTTAGGACAACGTTTAGCCAAAACGCTATTAGCCCAGAATAATGTTCA
TATTGATGATTTAATTTTAATTGATGTTGTTAAGCCTATTGCGCCAAATAATGATCCTCGTGTTCGATGTTATGAAATGA
ATTTACGTTATCCAACAGGATTAGATGAATTAATTACTGAAGAAACGGATGCTATTTTTCATTTAGCAGCTATCGTAAGT
AGTCATGCGGAACAAGATCCTGATTTAGGTTATGAAACTAATTTCTTAGCAACACGAAATATCCTTGAAATTTGTCGCAA
AAATAATCCTAAAGTTCGGTTTATTTTCTCTAGTTCTTTAGCCATATTTGGTGGAGAACTACCTGAAACTATTTTAGACA
GTACAGCATTCACACCGCAATCTACCTATGGTACACAAAAAGCAATGTGTGAATTGCTGATTAATGATTACTCACGAAAA
GGCTTTGTAGATGGTATTGTTGTTCGTTTACCAACCATTTGTATTCGACCAGGCAAACCAAACAAAGCTGCATCCTCATT
TGTTAGCAGTATTATGCGTGAACCTCTACATGGGGAGGATGCCGTGTGTCCTGTATCGGAAGAATTACGTTTATGGTTAT
CTAGCCCAAATACAGTAGTTGCAAACTTTATTCATGCTTTACAACTTCCATCACTTCCATTGCGTAGTTGGCATACTATT
AATCTTCCAGGGTTTAGTGTTACCGTAAAACAAATGTTATCTGATCTCACTCAAGTAAAAGGTGAAGCAATTTTAGAACA
TATTAAATTTGAGTTCGATGAAAGCATTAACAATATCGTTGCTAGTTGGCCTTCAAGAATTGATAACACCCAGGCATTAG
CTCTTGGTTTCAAAGTGGATAGCAATTTCCAAAATGTTATCCAACAGTTTATTGAATATGATATGTAG

Upstream 100 bases:

>100_bases
GGCGAAACCGTAACGGGATTGGATTGGTTTCAGAAGTATAAATAAATATTCTTAAATTTAACTGCGGTTTTAAAAACACA
TATTATCTAAGGAGGATAAA

Downstream 100 bases:

>100_bases
GAGGCACTATGTTTGGCTTACCGATTCCAATTATTGGATTATTAATCGCTGTATTTGTTCTCGTTTTTCTCGTATTAAGA
ACGCGAGTTCATGCTTTCAT

Product: hypothetical protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKVVITGGQGFLGQRLAKTLLAQNNVHIDDLILIDVVKPIAPNNDPRVRCYEMNLRYPTGLDELITEETDAIFHLAAIVS
SHAEQDPDLGYETNFLATRNILEICRKNNPKVRFIFSSSLAIFGGELPETILDSTAFTPQSTYGTQKAMCELLINDYSRK
GFVDGIVVRLPTICIRPGKPNKAASSFVSSIMREPLHGEDAVCPVSEELRLWLSSPNTVVANFIHALQLPSLPLRSWHTI
NLPGFSVTVKQMLSDLTQVKGEAILEHIKFEFDESINNIVASWPSRIDNTQALALGFKVDSNFQNVIQQFIEYDM

Sequences:

>Translated_315_residues
MKVVITGGQGFLGQRLAKTLLAQNNVHIDDLILIDVVKPIAPNNDPRVRCYEMNLRYPTGLDELITEETDAIFHLAAIVS
SHAEQDPDLGYETNFLATRNILEICRKNNPKVRFIFSSSLAIFGGELPETILDSTAFTPQSTYGTQKAMCELLINDYSRK
GFVDGIVVRLPTICIRPGKPNKAASSFVSSIMREPLHGEDAVCPVSEELRLWLSSPNTVVANFIHALQLPSLPLRSWHTI
NLPGFSVTVKQMLSDLTQVKGEAILEHIKFEFDESINNIVASWPSRIDNTQALALGFKVDSNFQNVIQQFIEYDM
>Mature_315_residues
MKVVITGGQGFLGQRLAKTLLAQNNVHIDDLILIDVVKPIAPNNDPRVRCYEMNLRYPTGLDELITEETDAIFHLAAIVS
SHAEQDPDLGYETNFLATRNILEICRKNNPKVRFIFSSSLAIFGGELPETILDSTAFTPQSTYGTQKAMCELLINDYSRK
GFVDGIVVRLPTICIRPGKPNKAASSFVSSIMREPLHGEDAVCPVSEELRLWLSSPNTVVANFIHALQLPSLPLRSWHTI
NLPGFSVTVKQMLSDLTQVKGEAILEHIKFEFDESINNIVASWPSRIDNTQALALGFKVDSNFQNVIQQFIEYDM

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI32566934, Length=301, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24667531, Length=296, Percent_Identity=23.9864864864865, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1014_HAEIN (P44094)

Other databases:

- EMBL:   L42023
- PIR:   D64018
- RefSeq:   NP_439175.1
- ProteinModelPortal:   P44094
- SMR:   P44094
- GeneID:   950006
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1014
- NMPDR:   fig|71421.1.peg.978
- TIGR:   HI_1014
- HOGENOM:   HBG376194
- OMA:   VFGAPFP
- ProtClustDB:   CLSK870462
- BioCyc:   HINF71421:HI_1014-MONOMER
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01370 Epimerase

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35146; Mature: 35146

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVITGGQGFLGQRLAKTLLAQNNVHIDDLILIDVVKPIAPNNDPRVRCYEMNLRYPTG
CEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHEEHHHHCCCCCCCCEEEEEECCCCCCC
LDELITEETDAIFHLAAIVSSHAEQDPDLGYETNFLATRNILEICRKNNPKVRFIFSSSL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCH
AIFGGELPETILDSTAFTPQSTYGTQKAMCELLINDYSRKGFVDGIVVRLPTICIRPGKP
HHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCC
NKAASSFVSSIMREPLHGEDAVCPVSEELRLWLSSPNTVVANFIHALQLPSLPLRSWHTI
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCHHHHHHHHHHCCCCCCCCCCEEE
NLPGFSVTVKQMLSDLTQVKGEAILEHIKFEFDESINNIVASWPSRIDNTQALALGFKVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
SNFQNVIQQFIEYDM
CCHHHHHHHHHHCCC
>Mature Secondary Structure
MKVVITGGQGFLGQRLAKTLLAQNNVHIDDLILIDVVKPIAPNNDPRVRCYEMNLRYPTG
CEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHEEHHHHCCCCCCCCEEEEEECCCCCCC
LDELITEETDAIFHLAAIVSSHAEQDPDLGYETNFLATRNILEICRKNNPKVRFIFSSSL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCH
AIFGGELPETILDSTAFTPQSTYGTQKAMCELLINDYSRKGFVDGIVVRLPTICIRPGKP
HHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCC
NKAASSFVSSIMREPLHGEDAVCPVSEELRLWLSSPNTVVANFIHALQLPSLPLRSWHTI
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCHHHHHHHHHHCCCCCCCCCCEEE
NLPGFSVTVKQMLSDLTQVKGEAILEHIKFEFDESINNIVASWPSRIDNTQALALGFKVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
SNFQNVIQQFIEYDM
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800