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The map label for this gene is leuD [H]

Identifier: 16272927

GI number: 16272927

Start: 1047673

End: 1048275

Strand: Direct

Name: leuD [H]

Synonym: HI0989

Alternate gene names: 16272927

Gene position: 1047673-1048275 (Clockwise)

Preceding gene: 16272926

Following gene: 16272934

Centisome position: 57.25

GC content: 39.8

Gene sequence:

>603_bases
ATGGCAGGATTTAAACAACTTTCAGGCTTAGTCGTACCATTGGATGCGGCAAATGTAGATACCGATGCAATTATTCCAAA
ACAATTTTTACAAGCCATTACCCGCATAGGTTTCGGCAAACACTTATTCCACGAATGGCGTTATTTGGACGTAGAAGGCA
CCAAGCCAAATCCAGAATTTGTCTTAAATTATCCGCAATATCAAGGTGCAACAATTTTGTTGGCGCGTAAAAACCTTGGC
TGTGGTTCTTCTCGTGAACACGCCCCTTGGGCATTAGCAGACTACGGCTTTAAAGTAATGATCGCCCCAAGTTTTGCAGA
TATTTTCTATAACAATAGCTTAAATAATCATATGTTACCGATTCGTTTAAGCGAAGAAGAAGTAGAAGAAATCTTCCAAT
GGGTATGGGCAAATGAAGGCAAACAAATCCACGTAGATTTAGAAGCGATGACGGTCACTGTAGGGGATAAAGTTTATACT
TTTGAGCTTGATGAATTCCGCCGCCATTGTTTGCTAAACGGTTTAGATAATATCGGCTTAACTCTTCAGCACGAAGACAA
AATCTCAGCATACGAGAAAAATATTCCAGCGTTTTTACGTTAA

Upstream 100 bases:

>100_bases
TGGTAAGTCCTGCAATGGCGGCAGCGGCAGGAGTGTTCGGTAAATTTGTTGATATTCGTGACGTAACATTAAACTAAGGC
TCAAAAGAGGAAAAAGAAAA

Downstream 100 bases:

>100_bases
AAATAAAAAATGCGCTGATTTTTCACCGCACTTAATCACTGAATTTCATTCAAAAATAAAGCCCTGTAAGACACAGGGCT
TTGTTGTTTATAACGTTAAT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MAGFKQLSGLVVPLDAANVDTDAIIPKQFLQAITRIGFGKHLFHEWRYLDVEGTKPNPEFVLNYPQYQGATILLARKNLG
CGSSREHAPWALADYGFKVMIAPSFADIFYNNSLNNHMLPIRLSEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGDKVYT
FELDEFRRHCLLNGLDNIGLTLQHEDKISAYEKNIPAFLR

Sequences:

>Translated_200_residues
MAGFKQLSGLVVPLDAANVDTDAIIPKQFLQAITRIGFGKHLFHEWRYLDVEGTKPNPEFVLNYPQYQGATILLARKNLG
CGSSREHAPWALADYGFKVMIAPSFADIFYNNSLNNHMLPIRLSEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGDKVYT
FELDEFRRHCLLNGLDNIGLTLQHEDKISAYEKNIPAFLR
>Mature_199_residues
AGFKQLSGLVVPLDAANVDTDAIIPKQFLQAITRIGFGKHLFHEWRYLDVEGTKPNPEFVLNYPQYQGATILLARKNLGC
GSSREHAPWALADYGFKVMIAPSFADIFYNNSLNNHMLPIRLSEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGDKVYTF
ELDEFRRHCLLNGLDNIGLTLQHEDKISAYEKNIPAFLR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=196, Percent_Identity=69.3877551020408, Blast_Score=300, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6321429, Length=197, Percent_Identity=51.7766497461929, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22812; Mature: 22681

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGFKQLSGLVVPLDAANVDTDAIIPKQFLQAITRIGFGKHLFHEWRYLDVEGTKPNPEF
CCCCHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHEEEEEECCCCCCCCE
VLNYPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIAPSFADIFYNNSLNNHMLP
EEECCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCEEEEECCCCCCCEEE
IRLSEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGDKVYTFELDEFRRHCLLNGLDNIGL
EEECHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCEEEEEEHHHHHHHHHHCCCCCCCE
TLQHEDKISAYEKNIPAFLR
EEEECCHHHHHHCCCCHHCC
>Mature Secondary Structure 
AGFKQLSGLVVPLDAANVDTDAIIPKQFLQAITRIGFGKHLFHEWRYLDVEGTKPNPEF
CCCHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHEEEEEECCCCCCCCE
VLNYPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIAPSFADIFYNNSLNNHMLP
EEECCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCEEEEECCCCCCCEEE
IRLSEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGDKVYTFELDEFRRHCLLNGLDNIGL
EEECHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCEEEEEEHHHHHHHHHHCCCCCCCE
TLQHEDKISAYEKNIPAFLR
EEEECCHHHHHHCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA