Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 16272926

GI number: 16272926

Start: 1046240

End: 1047649

Strand: Direct

Name: leuC [H]

Synonym: HI0988

Alternate gene names: 16272926

Gene position: 1046240-1047649 (Clockwise)

Preceding gene: 16272925

Following gene: 16272927

Centisome position: 57.17

GC content: 43.55

Gene sequence:

>1410_bases
ATGGCAAAAACACTTTACGAAAAATTATTTGATTCACACATTGTGTATGAAGCAGAGGGCGAAACGCCAATTTTGTATAT
TAATCGTCATTTGATTCACGAAGTAACCAGCCCGCAGGCCTTTGATGGGTTACGCGTCGCAAATCGCCAAGTGCGCCAAG
TGAATAAAACTTTTGGCACAATGGATCACAGCATTTCTACTCAAGTACGAGATGTCAATAAACTTGAAGGTCAAGCGAAA
ATTCAAGTGTTAGAATTGGATAAAAATACCAAAGCAACAGGTATTAAATTATTTGATATAACAACTAAAGAGCAAGGTAT
TGTACACGTTATGGGGCCAGAACAAGGTTTAACCTTACCCGGTATGACAATTGTTTGTGGCGATTCACACACCGCAACGC
ACGGTGCATTTGGTGCCTTGGCTTTTGGTATCGGTACTTCTGAAGTAGAGCACGTATTAGCCACTCAAACTTTAAAACAG
GCTCGCGCAAAAAGTATGAAAATTGAAGTGCGTGGTAAAGTCGCATCAGGTATTACGGCAAAAGATATTATTCTTGCTAT
TATCGGCAAAACTACGATGGCAGGCGGTACAGGACACGTTGTAGAATTCTGTGGCGAAGCGATCCAAGACCTTTCTATGG
AGGGGCGTATGACCGTCTGTAATATGGCAATTGAAATGGGTGCAAAAGCAGGCTTGATTGCGCCAGATGAAACCACATTT
GCATATTTGAAAGATCGTCCACACGCGCCGAAAGGCAAAGATTGGGAGGATGCCGTTGCTTATTGGAAAACCTTAAAATC
TGACGATGATGCACAATTTGACACTGTGGTAACGCTAGAAGCAAAAGACATCGCACCACAAGTCACTTGGGGGACAAACC
CCGGGCAGGTAATTTCTGTGAATGAAACCATACCAAATCCGCAAGAAATGGCAGACCCTGTACAACGTGCTTCGGCGGAA
AAAGCCTTACATTATATTGGCTTGGAAGCTGGCACTAATTTAAAAGATATTAAAGTTGATCAAGTGTTCATCGGATCTTG
CACCAACTCGCGCATTGAAGATTTACGCGCAGCGGCGGCTGTGATGAAAGGCCGTAAAAAAGCTGATAACGTAAAACGTA
TTTTAGTAGTACCAGGTTCTGGCTTAGTGAAAGAACAAGCGGAAAAAGAGGGCTTGGATAAAATCTTTATTGCCGCAGGT
GCAGAATGGCGTAATCCAGGTTGTTCAATGTGTTTAGGAATGAACGACGACCGTTTAGGCGAATGGGAACGCTGTGCTTC
CACTTCAAACCGTAACTTTGAAGGTCGCCAAGGCCGTAATGGACGCACCCATTTGGTAAGTCCTGCAATGGCGGCAGCGG
CAGGAGTGTTCGGTAAATTTGTTGATATTCGTGACGTAACATTAAACTAA

Upstream 100 bases:

>100_bases
AAATATCCCCAATAAACAGGGGGGACAAAGTATTTAAAGTGCGGTTGAAAAACACATCGAATTTTAACCGCACTTCCCTT
AACGAATAGAGAAAAATATT

Downstream 100 bases:

>100_bases
GGCTCAAAAGAGGAAAAAGAAAAATGGCAGGATTTAAACAACTTTCAGGCTTAGTCGTACCATTGGATGCGGCAAATGTA
GATACCGATGCAATTATTCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAKTLYEKLFDSHIVYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGTMDHSISTQVRDVNKLEGQAK
IQVLELDKNTKATGIKLFDITTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
ARAKSMKIEVRGKVASGITAKDIILAIIGKTTMAGGTGHVVEFCGEAIQDLSMEGRMTVCNMAIEMGAKAGLIAPDETTF
AYLKDRPHAPKGKDWEDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISVNETIPNPQEMADPVQRASAE
KALHYIGLEAGTNLKDIKVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKEGLDKIFIAAG
AEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN

Sequences:

>Translated_469_residues
MAKTLYEKLFDSHIVYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGTMDHSISTQVRDVNKLEGQAK
IQVLELDKNTKATGIKLFDITTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
ARAKSMKIEVRGKVASGITAKDIILAIIGKTTMAGGTGHVVEFCGEAIQDLSMEGRMTVCNMAIEMGAKAGLIAPDETTF
AYLKDRPHAPKGKDWEDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISVNETIPNPQEMADPVQRASAE
KALHYIGLEAGTNLKDIKVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKEGLDKIFIAAG
AEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN
>Mature_468_residues
AKTLYEKLFDSHIVYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGTMDHSISTQVRDVNKLEGQAKI
QVLELDKNTKATGIKLFDITTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQA
RAKSMKIEVRGKVASGITAKDIILAIIGKTTMAGGTGHVVEFCGEAIQDLSMEGRMTVCNMAIEMGAKAGLIAPDETTFA
YLKDRPHAPKGKDWEDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISVNETIPNPQEMADPVQRASAEK
ALHYIGLEAGTNLKDIKVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKEGLDKIFIAAGA
EWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=365, Percent_Identity=27.3972602739726, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI8659555, Length=447, Percent_Identity=26.3982102908277, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI41352693, Length=378, Percent_Identity=26.1904761904762, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=76.1802575107296, Blast_Score=728, Evalue=0.0,
Organism=Escherichia coli, GI87081781, Length=325, Percent_Identity=26.4615384615385, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=25.414364640884, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367097, Length=354, Percent_Identity=25.7062146892655, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=362, Percent_Identity=29.8342541436464, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=29.7520661157025, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=29.9342105263158, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=462, Percent_Identity=25.974025974026, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=60.9808102345416, Blast_Score=588, Evalue=1e-169,
Organism=Saccharomyces cerevisiae, GI6323335, Length=406, Percent_Identity=29.064039408867, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=369, Percent_Identity=28.9972899728997, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320440, Length=467, Percent_Identity=25.2676659528908, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281365315, Length=362, Percent_Identity=25.9668508287293, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17864292, Length=362, Percent_Identity=25.9668508287293, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161076999, Length=363, Percent_Identity=25.8953168044077, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=365, Percent_Identity=26.027397260274, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24645686, Length=460, Percent_Identity=26.5217391304348, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI17137564, Length=459, Percent_Identity=27.2331154684096, Blast_Score=95, Evalue=9e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50875; Mature: 50744

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKLFDSHIVYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGT
CCHHHHHHHCCCCEEEEECCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCC
MDHSISTQVRDVNKLEGQAKIQVLELDKNTKATGIKLFDITTKEQGIVHVMGPEQGLTLP
CCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQARAKSMKIEVRGKVASGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCH
KDIILAIIGKTTMAGGTGHVVEFCGEAIQDLSMEGRMTVCNMAIEMGAKAGLIAPDETTF
HHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AYLKDRPHAPKGKDWEDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISV
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCEEEECCCCCEEEEE
NETIPNPQEMADPVQRASAEKALHYIGLEAGTNLKDIKVDQVFIGSCTNSRIEDLRAAAA
CCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
VMKGRKKADNVKRILVVPGSGLVKEQAEKEGLDKIFIAAGAEWRNPGCSMCLGMNDDRLG
HHHCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCC
EWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN
HHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEEEEEECC
>Mature Secondary Structure 
AKTLYEKLFDSHIVYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGT
CHHHHHHHCCCCEEEEECCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCC
MDHSISTQVRDVNKLEGQAKIQVLELDKNTKATGIKLFDITTKEQGIVHVMGPEQGLTLP
CCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQARAKSMKIEVRGKVASGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCH
KDIILAIIGKTTMAGGTGHVVEFCGEAIQDLSMEGRMTVCNMAIEMGAKAGLIAPDETTF
HHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AYLKDRPHAPKGKDWEDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISV
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCEEEECCCCCEEEEE
NETIPNPQEMADPVQRASAEKALHYIGLEAGTNLKDIKVDQVFIGSCTNSRIEDLRAAAA
CCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
VMKGRKKADNVKRILVVPGSGLVKEQAEKEGLDKIFIAAGAEWRNPGCSMCLGMNDDRLG
HHHCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCC
EWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN
HHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA