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The map label for this gene is glyS [H]

Identifier: 16272861

GI number: 16272861

Start: 981597

End: 983663

Strand: Reverse

Name: glyS [H]

Synonym: HI0924

Alternate gene names: 16272861

Gene position: 983663-981597 (Counterclockwise)

Preceding gene: 16272862

Following gene: 16272857

Centisome position: 53.75

GC content: 44.75

Gene sequence:

>2067_bases
ATGACAACCCAAAACTTCCTTGTAGAAATCGGCACGGAAGAGCTGCCACCAAAAGCTCTCAAAACATTAGCAACCTCTTT
TGCGGATAACGTTGAGGCGGAATTGAATCAAGCAGGTTTATCATTCGACAAAATCGAATGGTTTGCGGCGCCGCGTCGTT
TGGCGGTGAAAGTGTTGAACTTAGCCACACAGCAACCGAGCAAAGAAATCGAAAAACGCGGGCCTGCAGTGTCAGCAGCC
TTTGATGCGGAAGGTAAACCAACAAAAGCGGCTGAAGGCTGGGCACGTGGTTGTGGTATTACCGTTGAGCAAGCAGAACG
CATTGCAACTGATAAAGGCGAATGGCTCATTCATCGTGCGAAAATTGAAGGTCAGCCGACCAAAAACTTACTTAACGATA
TTGTGGCAAATGCGTTAGCGAAATTGCCAATTCCAAAACCAATGCGTTGGGCAGATAAAACCGTGCAGTTTATTCGCCCA
GTTCACACTGTGACTATGTTGTTAGGCGATGAGTTAATTGAAGGCGAAATTTTAGGTGTGGCAAGTGCTCGCACCATTCG
CGGTCACCGTTTCTTAGGCGAGAAAGAATTTTACATTCAACATGCAGACCAATATCCGCAATTATTGCGTGAAAAAGGTT
CTGTGGTGGCGGACTTCAACGAGCGTAAAGCAGAAATTCTTGCAAAATCTCAAGCAAAAGCGACCGCACTTGGCGGCGTA
GCTGATATTGAAGAAAGCTTGCTTGAAGAAGTCACTTCCTTGGTGGAATATCCAAATGTTTTAGCAGCAAAATTTGAAGA
ACATTTCTTAGCAGTGCCTGCGGAAGCCTTGGTTTACACCATGAAAGGAGACCAAAAATATTTCCCAATTTATGACAAAG
ACGGCAAATTATTACCGCACTTTATTTTTGTCTCGAACATCAACCCAGAAGATCCAACCGCGATTATCGAAGGGAATGAA
AAAGTGGTTCGTCCACGTTTAACCGATGCGGAATTCTTCTTCAAAACCGACTTAAAACAAAAACTGGTTGATCGTTTACC
ACGTTTAGAAACCGTGTTGTTCCAGCAACAATTGGGTACATTGAAAGATAAAACTGATCGCATTGAGCAACTTGCAGGCG
AAATTGCAAAACAAATCGGCGCAGACGAAGCGAAAGCAAAACGTGCGGGTTTACTGTCAAAATGTGATTTGATGACCAAC
ATGGTATTTGAATTCACTGATACGCAAGGCGTAATGGGTATGCACTACGCTCGTCATGACGGTGAAGATGAAGAAGTAGC
GGTGGCGTTAAACGAACAATATATGCCACGCTTTGCTGGTGATGAATTACCAAAATCTCTCGTTGCAAGTGCGGTCGCTT
TAGCGGATAAATTTGACACTTTAACGGGTATCTTCGGCATCGGCCAAGCACCAAAAGGTAGCGCAGACCCATTTGCATTA
CGTCGTGCGGCATTAGGTGCGTTACGTATTATCGTAGAGAAAAACTTACCACTTGATTTAGAAGATTTAGTGAAAAAATC
AGCCGCACTTTTCGGTGATAAACTCACGAATCAAAATGTGGTTGCTGATGTGGTGGACTTCATGCTCGGTCGTTTCCGTG
CATGGTATCAAGATGAAGGCATTGCGGTGGATGTGATTCAAGCGGTATTGGCACGTCGTCCAACTCGCCCAGCAGACTTT
GATGCGCGCGTGCGTGCGGTTTCACACTTCCGTACTTTAGATTCAGCGGAAGCGTTAGCAGCAGCAAACAAACGTGTCAG
CAACATCTTAGCGAAAGCAGGTGCGGCAATTGGCGAGATCAATTTAACCGCTTGCGTAGAGCCAGCAGAAAAAGCCCTTG
CTGAAGCGGTGCTTGCATTACGCACTGAAGTACAACCGCTTATCGCACAAGGCGATTACACGACAGTATTGGATAAATTA
GCAAACTTACGTGCGCCAGTAGACAGTTTCTTTGATAACGTGATGGTAAATGCTGAAGATCCAGCACTACGTCAAAACCG
ATTAGCGATTTTAAATACGTTACAAGGGTTATTCTTACAAGTAGCTGATATTTCAGTGCTTCAATAA

Upstream 100 bases:

>100_bases
AATCCCCTCTTTAGCAAAGAGGGGTAGGGGCGATTTGGCAGAAATGATAACGAGAAAATAAGAATTTAAATCTACAGAAT
TTTTAAATAAGAGAAACAAA

Downstream 100 bases:

>100_bases
TGTAAAAAGGGATGGTTAATTTTTCGTCAATCATCCCTTCTTTTATAGACTTCAAGTGATTATTTCTCTATAATTACCGC
ACTTTCCCCCCTTAGCTCAG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MTTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLSFDKIEWFAAPRRLAVKVLNLATQQPSKEIEKRGPAVSAA
FDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLIHRAKIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRP
VHTVTMLLGDELIEGEILGVASARTIRGHRFLGEKEFYIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGV
ADIEESLLEEVTSLVEYPNVLAAKFEEHFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPEDPTAIIEGNE
KVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGTLKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
RRAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEGIAVDVIQAVLARRPTRPADF
DARVRAVSHFRTLDSAEALAAANKRVSNILAKAGAAIGEINLTACVEPAEKALAEAVLALRTEVQPLIAQGDYTTVLDKL
ANLRAPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISVLQ

Sequences:

>Translated_688_residues
MTTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLSFDKIEWFAAPRRLAVKVLNLATQQPSKEIEKRGPAVSAA
FDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLIHRAKIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRP
VHTVTMLLGDELIEGEILGVASARTIRGHRFLGEKEFYIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGV
ADIEESLLEEVTSLVEYPNVLAAKFEEHFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPEDPTAIIEGNE
KVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGTLKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
RRAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEGIAVDVIQAVLARRPTRPADF
DARVRAVSHFRTLDSAEALAAANKRVSNILAKAGAAIGEINLTACVEPAEKALAEAVLALRTEVQPLIAQGDYTTVLDKL
ANLRAPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISVLQ
>Mature_687_residues
TTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLSFDKIEWFAAPRRLAVKVLNLATQQPSKEIEKRGPAVSAAF
DAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLIHRAKIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRPV
HTVTMLLGDELIEGEILGVASARTIRGHRFLGEKEFYIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGVA
DIEESLLEEVTSLVEYPNVLAAKFEEHFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPEDPTAIIEGNEK
VVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGTLKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFALR
RAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEGIAVDVIQAVLARRPTRPADFD
ARVRAVSHFRTLDSAEALAAANKRVSNILAKAGAAIGEINLTACVEPAEKALAEAVLALRTEVQPLIAQGDYTTVLDKLA
NLRAPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISVLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=69.66618287373, Blast_Score=971, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75614; Mature: 75482

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLSFDKIEWFAAPRRLAVKVLN
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LATQQPSKEIEKRGPAVSAAFDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLIHRA
HHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
KIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRPVHTVTMLLGDELIEGEILGV
ECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ASARTIRGHRFLGEKEFYIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGV
CCHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHCCH
ADIEESLLEEVTSLVEYPNVLAAKFEEHFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPEDPTAIIEGNEKVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGT
EEEEECCCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEG
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
IAVDVIQAVLARRPTRPADFDARVRAVSHFRTLDSAEALAAANKRVSNILAKAGAAIGEI
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCE
NLTACVEPAEKALAEAVLALRTEVQPLIAQGDYTTVLDKLANLRAPVDSFFDNVMVNAED
EHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCEEECCCC
PALRQNRLAILNTLQGLFLQVADISVLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQNFLVEIGTEELPPKALKTLATSFADNVEAELNQAGLSFDKIEWFAAPRRLAVKVLN
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LATQQPSKEIEKRGPAVSAAFDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLIHRA
HHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
KIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRPVHTVTMLLGDELIEGEILGV
ECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ASARTIRGHRFLGEKEFYIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGV
CCHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHCCH
ADIEESLLEEVTSLVEYPNVLAAKFEEHFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPEDPTAIIEGNEKVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGT
EEEEECCCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGALRIIVEKNLPLDLEDLVKKSAALFGDKLTNQNVVADVVDFMLGRFRAWYQDEG
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
IAVDVIQAVLARRPTRPADFDARVRAVSHFRTLDSAEALAAANKRVSNILAKAGAAIGEI
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCE
NLTACVEPAEKALAEAVLALRTEVQPLIAQGDYTTVLDKLANLRAPVDSFFDNVMVNAED
EHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCEEECCCC
PALRQNRLAILNTLQGLFLQVADISVLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA