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The map label for this gene is purD

Identifier: 16272828

GI number: 16272828

Start: 941550

End: 942839

Strand: Direct

Name: purD

Synonym: HI0888

Alternate gene names: 16272828

Gene position: 941550-942839 (Clockwise)

Preceding gene: 16272827

Following gene: 16272829

Centisome position: 51.45

GC content: 44.5

Gene sequence:

>1290_bases
ATGAACATCCTCATCATCGGAAACGGCGGTCGTGAACACGCCTTGGCTTGGAAAGTTGCACAATCTCCATTAGCAGATAA
AGTTTTTGTCGCACCGGGTAACGCTGGTACTGCATTGGAACACAAAGTAGAAAACGTGAATATTTCTGCGACAGATATTC
CAGCATTAGTGAAATTTGCTCAAGATAAACAAATTGGATTAACCATTGTTGGTCCTGAAGCCCCCTTAGTGATTGGGGTA
GTTGATGCATTTCGTGAAGCTGGATTGAAGATTTTTGGGCCAGCTAAAGCGGCTGCGCAATTGGAAGGTTCAAAGGCATT
CACTAAAGATTTTCTCGCTCGTCATAAAATTCCAACAGCGGAATATCAAAACTTCACCGAAGTCGAACCTGCATTGACTT
ACTTGCGTGAAAAAGGCACACCAATTGTCGTTAAAGCGGATGGCTTGGCGGCGGGTAAAGGCGTAATCGTTGCGATGACA
TTGCAAGAAGCCGAAGATGCAGTACGCGATATGCTTTCTGGTAATGCCTTTGGCGAAGCGGGCAGCCGAGTAGTGATTGA
AGAATTTCTAGATGGCGAAGAAGCAAGTTTTATCGTCATGGTAGATGGTAAAAACGTAGAACCTATGGCAAGCAGTCAAG
ATCATAAACGCGTGGGCGAAAATGATACAGGGTTAAATACAGGCGGTATGGGCGCATATTCGCCTGCACCAGTAATAACA
CCCGAAATTCATAGTCGCATTATGAAGGAAGTGATTTACCCAACCGTTAATGGCATGGCAGCGGAAGGCAATGTTTACAC
GGGTTTCCTATATGCAGGATTAATGATTATGCCAAATGGGCAGCCGAAAGTGATCGAATTTAACTGCCGTTTTGGCGATC
CAGAAACGCAGCCAATAATGCTACGTCTCAAATCAGACCTTGTTGAACTCTGTCTTAAAGCATGTGACGGCAAATTAGAT
GAAGTAATATCACAATGGGATCTGCGAGCTTCTTTAGGTATCGTATTGGCTGCAGAGGGATACCCAAAAGATTATCGCAA
AGGCGATGAAATCAGCGGATTGCCTAAAAGTGCGGTCAAAAACGAGAAAGTTTTCTTAGCGGGTGTCGCAGAACAAGAAG
GCAAGCTAGTCACAAACGGCGGTCGTGTACTTTGTGTGACTGCGTTAGGCGAAAGTGTATTTGAAGCACAACAAAAAGCG
TTAAAATTGGCTGAGCAAATTCAATGGTCTGGGCGTTTTTATCGTCGAGACATTGGTTACAGGGCTGTGGAACGAGAGTT
GCAAAAATAA

Upstream 100 bases:

>100_bases
ATTAATTTATCTAACTATCCCCCTCTTTACAAAAGAGGGGGAATTGCAGGTAAAAAATAGCCGTACTTTTTTTAAATTAG
GCATACGCAAGGACTACAAC

Downstream 100 bases:

>100_bases
TCCATATCATTATGATGAATTAATTTCATTTAAGTGATGAAAATTTAGGAATGAAATTTTAATTAAATTTTTATCCATAA
ACAGTTAAACTATCTGCCAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNILIIGNGGREHALAWKVAQSPLADKVFVAPGNAGTALEHKVENVNISATDIPALVKFAQDKQIGLTIVGPEAPLVIGV
VDAFREAGLKIFGPAKAAAQLEGSKAFTKDFLARHKIPTAEYQNFTEVEPALTYLREKGTPIVVKADGLAAGKGVIVAMT
LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMASSQDHKRVGENDTGLNTGGMGAYSPAPVIT
PEIHSRIMKEVIYPTVNGMAAEGNVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMLRLKSDLVELCLKACDGKLD
EVISQWDLRASLGIVLAAEGYPKDYRKGDEISGLPKSAVKNEKVFLAGVAEQEGKLVTNGGRVLCVTALGESVFEAQQKA
LKLAEQIQWSGRFYRRDIGYRAVERELQK

Sequences:

>Translated_429_residues
MNILIIGNGGREHALAWKVAQSPLADKVFVAPGNAGTALEHKVENVNISATDIPALVKFAQDKQIGLTIVGPEAPLVIGV
VDAFREAGLKIFGPAKAAAQLEGSKAFTKDFLARHKIPTAEYQNFTEVEPALTYLREKGTPIVVKADGLAAGKGVIVAMT
LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMASSQDHKRVGENDTGLNTGGMGAYSPAPVIT
PEIHSRIMKEVIYPTVNGMAAEGNVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMLRLKSDLVELCLKACDGKLD
EVISQWDLRASLGIVLAAEGYPKDYRKGDEISGLPKSAVKNEKVFLAGVAEQEGKLVTNGGRVLCVTALGESVFEAQQKA
LKLAEQIQWSGRFYRRDIGYRAVERELQK
>Mature_429_residues
MNILIIGNGGREHALAWKVAQSPLADKVFVAPGNAGTALEHKVENVNISATDIPALVKFAQDKQIGLTIVGPEAPLVIGV
VDAFREAGLKIFGPAKAAAQLEGSKAFTKDFLARHKIPTAEYQNFTEVEPALTYLREKGTPIVVKADGLAAGKGVIVAMT
LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMASSQDHKRVGENDTGLNTGGMGAYSPAPVIT
PEIHSRIMKEVIYPTVNGMAAEGNVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMLRLKSDLVELCLKACDGKLD
EVISQWDLRASLGIVLAAEGYPKDYRKGDEISGLPKSAVKNEKVFLAGVAEQEGKLVTNGGRVLCVTALGESVFEAQQKA
LKLAEQIQWSGRFYRRDIGYRAVERELQK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=51.1792452830189, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=51.3002364066194, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=51.3002364066194, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=51.3002364066194, Blast_Score=421, Evalue=1e-118,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=75, Blast_Score=655, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=42.4882629107981, Blast_Score=318, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=41.2162162162162, Blast_Score=346, Evalue=3e-96,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.8139534883721, Blast_Score=351, Evalue=6e-97,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.8139534883721, Blast_Score=350, Evalue=8e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_HAEIN (P43845)

Other databases:

- EMBL:   L42023
- PIR:   C64100
- RefSeq:   NP_439049.1
- ProteinModelPortal:   P43845
- SMR:   P43845
- GeneID:   949893
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0888
- NMPDR:   fig|71421.1.peg.855
- TIGR:   HI_0888
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   HINF71421:HI_0888-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 46249; Mature: 46249

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIGNGGREHALAWKVAQSPLADKVFVAPGNAGTALEHKVENVNISATDIPALVKFA
CEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHCCCEEECCCHHHHHHHH
QDKQIGLTIVGPEAPLVIGVVDAFREAGLKIFGPAKAAAQLEGSKAFTKDFLARHKIPTA
CCCCCCEEEECCCCCEEEEHHHHHHHCCCEEEECCHHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTEVEPALTYLREKGTPIVVKADGLAAGKGVIVAMTLQEAEDAVRDMLSGNAFGEA
HCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGKNVEPMASSQDHKRVGENDTGLNTGGMGAYSPAPVIT
CCEEEHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHSRIMKEVIYPTVNGMAAEGNVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHCCCCCCEEECCCEEEEEEHCCEEEEECCCCEEEEEEEECCCCCCCCEE
LRLKSDLVELCLKACDGKLDEVISQWDLRASLGIVLAAEGYPKDYRKGDEISGLPKSAVK
EEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHC
NEKVFLAGVAEQEGKLVTNGGRVLCVTALGESVFEAQQKALKLAEQIQWSGRFYRRDIGY
CCEEEEEEECCCCCEEEECCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCH
RAVERELQK
HHHHHHHCC
>Mature Secondary Structure
MNILIIGNGGREHALAWKVAQSPLADKVFVAPGNAGTALEHKVENVNISATDIPALVKFA
CEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHCCCEEECCCHHHHHHHH
QDKQIGLTIVGPEAPLVIGVVDAFREAGLKIFGPAKAAAQLEGSKAFTKDFLARHKIPTA
CCCCCCEEEECCCCCEEEEHHHHHHHCCCEEEECCHHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTEVEPALTYLREKGTPIVVKADGLAAGKGVIVAMTLQEAEDAVRDMLSGNAFGEA
HCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGKNVEPMASSQDHKRVGENDTGLNTGGMGAYSPAPVIT
CCEEEHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHSRIMKEVIYPTVNGMAAEGNVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHCCCCCCEEECCCEEEEEEHCCEEEEECCCCEEEEEEEECCCCCCCCEE
LRLKSDLVELCLKACDGKLDEVISQWDLRASLGIVLAAEGYPKDYRKGDEISGLPKSAVK
EEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHC
NEKVFLAGVAEQEGKLVTNGGRVLCVTALGESVFEAQQKALKLAEQIQWSGRFYRRDIGY
CCEEEEEEECCCCCEEEECCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCH
RAVERELQK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800