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The map label for this gene is trmA

Identifier: 16272789

GI number: 16272789

Start: 894898

End: 895989

Strand: Direct

Name: trmA

Synonym: HI0848

Alternate gene names: 16272789

Gene position: 894898-895989 (Clockwise)

Preceding gene: 16272788

Following gene: 16273637

Centisome position: 48.9

GC content: 38.37

Gene sequence:

>1092_bases
ATGCAATTACCGATTTCTCAATACAACGAACTGCTACAAAAAAAACTTGAAAAATTAACCGCACTTTTACACCCTTTTAA
TGCACCAGATATTCAAGTTTTTGACTCTCCAACAAGTCATTATCGCATGCGTGCGGAGTTTCGTATTTGGCACGAGCAAG
ATGATTTTTACCATATTATGTTTGATCAAGCGACGCTGCAGCGTTATCGCGTGGATGAATTTCCAATCGCCAGCATGCTA
ATTAATCGTATGATGCAAACCTTATTACCTTTGTTAAAACAACAAGAAGTGCTACACAAAAAATTATTCCAAATTGATTA
CCTTAGCACCTTGAGTAATAAGATTATTGTGAGCTTGCTTTATCATAAAACACTCACTGAAGAATGGGAAAGTGCGGCTA
AAAATTTGAAAGATTTACTCGAAAAGCAAGATTTTGATGTGCAAATAATCGGGCGTGCAAGTAAACAAAAAATCTGTTTT
GAGCAAGATTATGTGGACGAAGTTTTACCTGTGAATGGCAGAAATTATGTCTATCGCCAAGTCGAGAATAGTTTTACCCA
GCCAAACGCAACGGTAAACTGCAAAATGCTTGAATGGGCAATTGATTGTACCCAAAATAGCGAAGGTGATTTATTAGAGC
TTTACTGCGGAAACGGTAATTTTTCTATCGCACTTGCACAAAATTTCCGTAAGGTATTGGCAACAGAAATCGCCAAACCT
TCCGTAGCCGCCGCACAATTTAATATTGCTGAAAATAAAGTGGATAATTTACAAATTATCCGTATGTCGGCAGAGGAATT
TACGCAGGCAATGAATGGGGTTAGAGCCTTTAACCGCTTGAAAGGGATTGATTTAAAATCTTATGAATGTAATACCATTT
TTGTCGATCCGCCTCGTGCGGGGCTTGATCCTGATACAGTGAAACTTGTACAAAATTACGATCGCATTTTGTATATTTCC
TGTAATCCACATACGCTGTGTGATAATCTTGTTGAACTATCAAAAACGCACCGCATTGAAAAAGCGGCGTTATTTGACCA
ATTCCCTTATACTGACCACATGGAAAGCGGCCTATGGCTCATTCGCAAGTAG

Upstream 100 bases:

>100_bases
AATAGCTTGTGAAATCACGCGTAATTCACGGCTGATAACTTTATTCTCCTATCTTTGTATGAACGATTCGTCTTCATACA
CCCTATGAATACTAATTTCT

Downstream 100 bases:

>100_bases
GAATTCAGCTGATTTCTGAATCGACAGAAAAAACATTAGCAGAATTGATCGCACTTTGTGCTGAACACAACATTATTCAT
AATGAAAAGAGCCCACTTGC

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQLPISQYNELLQKKLEKLTALLHPFNAPDIQVFDSPTSHYRMRAEFRIWHEQDDFYHIMFDQATLQRYRVDEFPIASML
INRMMQTLLPLLKQQEVLHKKLFQIDYLSTLSNKIIVSLLYHKTLTEEWESAAKNLKDLLEKQDFDVQIIGRASKQKICF
EQDYVDEVLPVNGRNYVYRQVENSFTQPNATVNCKMLEWAIDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIAKP
SVAAAQFNIAENKVDNLQIIRMSAEEFTQAMNGVRAFNRLKGIDLKSYECNTIFVDPPRAGLDPDTVKLVQNYDRILYIS
CNPHTLCDNLVELSKTHRIEKAALFDQFPYTDHMESGLWLIRK

Sequences:

>Translated_363_residues
MQLPISQYNELLQKKLEKLTALLHPFNAPDIQVFDSPTSHYRMRAEFRIWHEQDDFYHIMFDQATLQRYRVDEFPIASML
INRMMQTLLPLLKQQEVLHKKLFQIDYLSTLSNKIIVSLLYHKTLTEEWESAAKNLKDLLEKQDFDVQIIGRASKQKICF
EQDYVDEVLPVNGRNYVYRQVENSFTQPNATVNCKMLEWAIDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIAKP
SVAAAQFNIAENKVDNLQIIRMSAEEFTQAMNGVRAFNRLKGIDLKSYECNTIFVDPPRAGLDPDTVKLVQNYDRILYIS
CNPHTLCDNLVELSKTHRIEKAALFDQFPYTDHMESGLWLIRK
>Mature_363_residues
MQLPISQYNELLQKKLEKLTALLHPFNAPDIQVFDSPTSHYRMRAEFRIWHEQDDFYHIMFDQATLQRYRVDEFPIASML
INRMMQTLLPLLKQQEVLHKKLFQIDYLSTLSNKIIVSLLYHKTLTEEWESAAKNLKDLLEKQDFDVQIIGRASKQKICF
EQDYVDEVLPVNGRNYVYRQVENSFTQPNATVNCKMLEWAIDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIAKP
SVAAAQFNIAENKVDNLQIIRMSAEEFTQAMNGVRAFNRLKGIDLKSYECNTIFVDPPRAGLDPDTVKLVQNYDRILYIS
CNPHTLCDNLVELSKTHRIEKAALFDQFPYTDHMESGLWLIRK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=157, Percent_Identity=29.2993630573248, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI51173878, Length=157, Percent_Identity=29.2993630573248, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790403, Length=362, Percent_Identity=64.6408839779006, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI1789148, Length=176, Percent_Identity=31.25, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787083, Length=184, Percent_Identity=27.7173913043478, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666579, Length=417, Percent_Identity=23.5011990407674, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_HAEI8 (Q4QM53)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248554.1
- ProteinModelPortal:   Q4QM53
- SMR:   Q4QM53
- STRING:   Q4QM53
- GeneID:   3430317
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI1014
- eggNOG:   COG2265
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- PhylomeDB:   Q4QM53
- ProtClustDB:   PRK05031
- BioCyc:   HINF281310:NTHI1014-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 42231; Mature: 42231

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 321-321 BINDING 187-187 BINDING 215-215 BINDING 220-220 BINDING 236-236 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLPISQYNELLQKKLEKLTALLHPFNAPDIQVFDSPTSHYRMRAEFRIWHEQDDFYHIM
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHEEEEEEEEEEECCCCEEEEE
FDQATLQRYRVDEFPIASMLINRMMQTLLPLLKQQEVLHKKLFQIDYLSTLSNKIIVSLL
ECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHKTLTEEWESAAKNLKDLLEKQDFDVQIIGRASKQKICFEQDYVDEVLPVNGRNYVYRQ
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHHH
VENSFTQPNATVNCKMLEWAIDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIAKP
HHHCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHCCC
SVAAAQFNIAENKVDNLQIIRMSAEEFTQAMNGVRAFNRLKGIDLKSYECNTIFVDPPRA
CHHHHEEECHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEECCEEEECCCCC
GLDPDTVKLVQNYDRILYISCNPHTLCDNLVELSKTHRIEKAALFDQFPYTDHMESGLWL
CCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
IRK
EEC
>Mature Secondary Structure
MQLPISQYNELLQKKLEKLTALLHPFNAPDIQVFDSPTSHYRMRAEFRIWHEQDDFYHIM
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHEEEEEEEEEEECCCCEEEEE
FDQATLQRYRVDEFPIASMLINRMMQTLLPLLKQQEVLHKKLFQIDYLSTLSNKIIVSLL
ECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHKTLTEEWESAAKNLKDLLEKQDFDVQIIGRASKQKICFEQDYVDEVLPVNGRNYVYRQ
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHHH
VENSFTQPNATVNCKMLEWAIDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIAKP
HHHCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHCCC
SVAAAQFNIAENKVDNLQIIRMSAEEFTQAMNGVRAFNRLKGIDLKSYECNTIFVDPPRA
CHHHHEEECHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEECCEEEECCCCC
GLDPDTVKLVQNYDRILYISCNPHTLCDNLVELSKTHRIEKAALFDQFPYTDHMESGLWL
CCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
IRK
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA