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The map label for this gene is por

Identifier: 16272787

GI number: 16272787

Start: 893740

End: 894357

Strand: Direct

Name: por

Synonym: HI0846

Alternate gene names: 16272787

Gene position: 893740-894357 (Clockwise)

Preceding gene: 16272786

Following gene: 16272788

Centisome position: 48.83

GC content: 35.6

Gene sequence:

>618_bases
ATGAAAAAAGTATTACTTGCGTTAGGTTTAGGTGTCAGCACGCTTATGTCTGTAAATAGTTTTGCCGCAGATTTACAAGA
AGGCAAACAATATGTTCAAGTGAGTCAACAGGCTTCACAGCAAAAAGAAGTGATTGAGTTTTTCTCATTCTATTGCCCGC
ATTGTTACGCCTTTGAAATGGAATACAAAATTCCACAACAAGTCGTAGATGCTTTACCAAAAGATGTGAAATTTAAACAA
TATCATGTAAATTTCTTAGGTCATCAATCTGAAAACTTAACACGTGCTTGGGCGTTAGCAATGGCATTAGGTGCAGAAAG
TAAAGTAAAATCACCATTATTTGAAGCGGCTCAAAAGGATGCCTTGAAATCAATGGATGATATTCGAGCTATCTTCTTAT
CGAATGGCATAACCGCCGAGCAATTTGATGGTGGCATTAATAGTTTTGCAGTAAATGGTTTAGTTAATAAACAAGTGAAT
GCAGCAGAACAATTTAAAGTGCGTGGCGTACCTGATTTTTATGTAAATGGAAAATTCCGTGTAAACCCTGAAGGGTTAAA
TTATGATGATTTCGTGAAAGATTATGTGCAAACCGTAAAAGGTTTATTGCAAAAATAA

Upstream 100 bases:

>100_bases
GATAAATATGAGCCAATTCCTGGTCTCAAAAAAGATTACGAAGAAGATTTTAAAACCGCATTGCTCCGTGCTCGCGGTAT
CATTAAATAAGAGGAAAGAA

Downstream 100 bases:

>100_bases
CGAAAAATTGGTTTAATGCCAGCCCTATTTTGTAAATATTAATGAAAGAAGAGAGAATATTATGGCTGCAAGTTTTAGCG
TAACGCGTCGTTTCTTTGAC

Product: periplasmic oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKKVLLALGLGVSTLMSVNSFAADLQEGKQYVQVSQQASQQKEVIEFFSFYCPHCYAFEMEYKIPQQVVDALPKDVKFKQ
YHVNFLGHQSENLTRAWALAMALGAESKVKSPLFEAAQKDALKSMDDIRAIFLSNGITAEQFDGGINSFAVNGLVNKQVN
AAEQFKVRGVPDFYVNGKFRVNPEGLNYDDFVKDYVQTVKGLLQK

Sequences:

>Translated_205_residues
MKKVLLALGLGVSTLMSVNSFAADLQEGKQYVQVSQQASQQKEVIEFFSFYCPHCYAFEMEYKIPQQVVDALPKDVKFKQ
YHVNFLGHQSENLTRAWALAMALGAESKVKSPLFEAAQKDALKSMDDIRAIFLSNGITAEQFDGGINSFAVNGLVNKQVN
AAEQFKVRGVPDFYVNGKFRVNPEGLNYDDFVKDYVQTVKGLLQK
>Mature_205_residues
MKKVLLALGLGVSTLMSVNSFAADLQEGKQYVQVSQQASQQKEVIEFFSFYCPHCYAFEMEYKIPQQVVDALPKDVKFKQ
YHVNFLGHQSENLTRAWALAMALGAESKVKSPLFEAAQKDALKSMDDIRAIFLSNGITAEQFDGGINSFAVNGLVNKQVN
AAEQFKVRGVPDFYVNGKFRVNPEGLNYDDFVKDYVQTVKGLLQK

Specific function: Involved in disulfide-bond formation. Required for HI transformation. Acts by transferring its disulfide bond to other proteins

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1790291, Length=210, Percent_Identity=45.2380952380952, Blast_Score=183, Evalue=9e-48,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DSBA_HAEIN (P31810)

Other databases:

- EMBL:   M94205
- EMBL:   L42023
- PIR:   B64098
- RefSeq:   NP_439006.1
- ProteinModelPortal:   P31810
- SMR:   P31810
- GeneID:   949506
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0846
- TIGR:   HI_0846
- HOGENOM:   HBG680642
- OMA:   CYQFEQV
- ProtClustDB:   CLSK870622
- BioCyc:   HINF71421:HI_0846-MONOMER
- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PIRSF:   PIRSF001488

Pfam domain/function: PF01323 DSBA; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 22964; Mature: 22964

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00194 THIOREDOXIN_1; PS51352 THIOREDOXIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLLALGLGVSTLMSVNSFAADLQEGKQYVQVSQQASQQKEVIEFFSFYCPHCYAFEM
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
EYKIPQQVVDALPKDVKFKQYHVNFLGHQSENLTRAWALAMALGAESKVKSPLFEAAQKD
CCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALKSMDDIRAIFLSNGITAEQFDGGINSFAVNGLVNKQVNAAEQFKVRGVPDFYVNGKFR
HHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCEEECCEEE
VNPEGLNYDDFVKDYVQTVKGLLQK
ECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVLLALGLGVSTLMSVNSFAADLQEGKQYVQVSQQASQQKEVIEFFSFYCPHCYAFEM
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
EYKIPQQVVDALPKDVKFKQYHVNFLGHQSENLTRAWALAMALGAESKVKSPLFEAAQKD
CCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALKSMDDIRAIFLSNGITAEQFDGGINSFAVNGLVNKQVNAAEQFKVRGVPDFYVNGKFR
HHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCEEECCEEE
VNPEGLNYDDFVKDYVQTVKGLLQK
ECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1438213; 7542800