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The map label for this gene is plsB [H]

Identifier: 16272689

GI number: 16272689

Start: 807394

End: 809826

Strand: Reverse

Name: plsB [H]

Synonym: HI0748

Alternate gene names: 16272689

Gene position: 809826-807394 (Counterclockwise)

Preceding gene: 16272692

Following gene: 16272685

Centisome position: 44.25

GC content: 35.96

Gene sequence:

>2433_bases
ATGGCAAATTTTATAAATATGTATCGCCAATTATTGAGCTTGCCGCTGTCTGCATTGGTGAAAAATAATCCTATTCCCGC
CAATCCAATAGAAGAGCTTTCACTTAATATTCATCAACCTATTGTTTATGTTTTGCCTTATACATCTCAAACTGATTTCG
TTATTTTCCGCCGAAATTGCTTAGCGTTAGGCTTGCCCGATCCCGCAGAGAAAAATGAAATTAATGGCGTTAAGTTACCA
CGTTATGTATATTTAGACGAAGGTCGCCGAATTTTTAAATCAAAAGGTGCGAAAGACGAAACCACTACCATTTTCAATAA
ATATCTTGAATTACACCGCACTTCTGAAAGTTTAGATGTGCAACTTATTCCTGTTTCTGTTCTTTGGGGACGTTCTCCCG
GACAAGAAGATAAATCAGATTTGCCTAATTTGCGTTTATTAAATGGTATACAAAAAACATTTGCAGCAATTTGGTTTGGA
CGTGATACTTTTGTGCGTTTTTCTCAAGCGGTTTCATTACGTTATATGGTAGTTGAACATGGCTCAGATGAAAAAATTGC
ACAAAAGCTGGCTCGAGTTGCAAAAATGCACTTCGCAAAACAACGTATTTCAGCGACAGGCCCTCGTTTACCAAATCGTC
AAGCAATGTTTAATAAATTATTGCAATCAGAAGCAATCCGACGAGCAATTGAAGATGAAGCAAAATCAAAAAATATCAGT
ATTGAAAAAGCACAAAAAGAAGCATATAAAATTTTGGATGAAATTGCAGCTGATGTCAGCCATTCAAGTTTACGTGCAGT
AGATCGTTTTTTACGTTGGCTTTGGAATAAACTTTATTCAGGTATTAATGTACAAAATTCGAATCGTGTCCGTAAATTAG
CGTTAGAAGGTCATGAAATTGTTTATGTGCCTTGCCACCGTAGTCATATCGATTACTTATTGCTTTCTTATGTGCTTTAT
CATCAAGGTCTTGTTCCGCCACATATCGCGGCAGGGATTAACTTAAATTTCTGGCCTATAGGTAGAATGTTTCGTAGTTG
GGGGGCATTCTTTATTCGCCGCACTTTTAAGGGAAATCGACTATATTCTGCCATTTTTCGCGAATATTTATCAGAACTAT
TCCATCGAGGCTATTCCGTCGAATATTTTATTGAGGGCGGTCGTTCTCGTACAGGTCGTTTGCTGGCACCAAAAACAGGT
ATGATGTCAATGACACTTCAAGCATTGCAACATAGTCAAACTCGCCCCATTTCGATTGTTCCTGTTTACGTTGGTTATGA
ACACGTATTAGAAGTAGACACTTATGCTAAGGAATTACGTGGTGCAGCGAAAGAAAAAGAAAATGCGGGTTTAGTACTTC
GTGTAATTAAAAAATTACGTAATCTTGGTCAAGGTTTTGTAAATTTTGGTGAGCCAATTACGCTTTCAAACTATTTGAGC
CAACATTTTCCTGATTGGAAAGAGCAAAATCACGAAGAAAAACCACAATGGTTTACACCTGCGGTAAATAATATTTCTAA
ACAAGTAATGATTAATATTAACAAAGCAGCGGCAGTTAATTCAATGAACCTTGTGGGTACAGCACTACTTTCATCTCGTC
AGCGAGCACTTTCACGTGAGCAACTGTTAGAACAACTTAGTAGTTATCAACAATTGTTACAAAATGTCCCTTATTCTACG
GACGTAGTGCTACCAAATGTGACACCGCAAGCAATGTTAGAACACGTATTGGCTTTAGATCGTATAGGTGTGTTAATTGA
AAAAGATAATTTTGGCGAAATTGTACGCTTAGAACGTTCATCTGCTGTACTAATGACTTATTATCGTAATAATATTCAGC
ATCTATTCGTATTACCATCTTTAGTTGCGAGTATTATTTTGCATTACGAAGCGATCCAAAAAGATTTATTATTAGACGCA
ATTAGAAAAATTTACCCTTTCTTACAAGGCGAATTGTTCTTACATTTCAATGAAGATGAATTGAATGTACAAATCCATCA
AATCATTAATGAATTTGCACGCCAAAGCGTTATTAATTCAAATGATAACTTCTTATCTATTAATAAATCAAAAGTACGAA
TTTTACAGCTTTGGTCTGCGGGAATGCTGGAAATCTTGCAACGTTATTACATTACTGTCACTATTTTACAAAAACAACCT
GCTATTTCTCGAGCTGAACTTGAAAAAGAAAGCCAGCTTGTTGCTCAACGTTTATCAGTATTACACGGCATAAATGCACC
AGAATTCTTTGATAAAGCGGTTTTTTCAAGCTTTATTGCAAACTTGAAAGAGCAACGTTACTTTGATGAATCTGGTTATA
CTGTTCTTGATAAAATAGAAGAATTAGCTTCAACGCTTTCCCATTTGATTTCAACAGAAATTTGCTTAACGGTGAAAGGA
ACAATTGAAAAATCCGAAGATCTTTCTTCATAA

Upstream 100 bases:

>100_bases
GGACGATAAAAACACCAAAGGGCTAAAAATAGTTCTATTCACAAACTGCTTTTTAAACGTCTATAAGATCTACAAACTTA
TAACAAAGAGAAAATTTATT

Downstream 100 bases:

>100_bases
AACAATACTGAGGGCATCATTGATGCCCTTTCTTTTATATAAATGATTTAGATCGCATTTAACGAAAAATTATACTTAAT
CAATGCAATTTTAATCTTGG

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MANFINMYRQLLSLPLSALVKNNPIPANPIEELSLNIHQPIVYVLPYTSQTDFVIFRRNCLALGLPDPAEKNEINGVKLP
RYVYLDEGRRIFKSKGAKDETTTIFNKYLELHRTSESLDVQLIPVSVLWGRSPGQEDKSDLPNLRLLNGIQKTFAAIWFG
RDTFVRFSQAVSLRYMVVEHGSDEKIAQKLARVAKMHFAKQRISATGPRLPNRQAMFNKLLQSEAIRRAIEDEAKSKNIS
IEKAQKEAYKILDEIAADVSHSSLRAVDRFLRWLWNKLYSGINVQNSNRVRKLALEGHEIVYVPCHRSHIDYLLLSYVLY
HQGLVPPHIAAGINLNFWPIGRMFRSWGAFFIRRTFKGNRLYSAIFREYLSELFHRGYSVEYFIEGGRSRTGRLLAPKTG
MMSMTLQALQHSQTRPISIVPVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGQGFVNFGEPITLSNYLS
QHFPDWKEQNHEEKPQWFTPAVNNISKQVMININKAAAVNSMNLVGTALLSSRQRALSREQLLEQLSSYQQLLQNVPYST
DVVLPNVTPQAMLEHVLALDRIGVLIEKDNFGEIVRLERSSAVLMTYYRNNIQHLFVLPSLVASIILHYEAIQKDLLLDA
IRKIYPFLQGELFLHFNEDELNVQIHQIINEFARQSVINSNDNFLSINKSKVRILQLWSAGMLEILQRYYITVTILQKQP
AISRAELEKESQLVAQRLSVLHGINAPEFFDKAVFSSFIANLKEQRYFDESGYTVLDKIEELASTLSHLISTEICLTVKG
TIEKSEDLSS

Sequences:

>Translated_810_residues
MANFINMYRQLLSLPLSALVKNNPIPANPIEELSLNIHQPIVYVLPYTSQTDFVIFRRNCLALGLPDPAEKNEINGVKLP
RYVYLDEGRRIFKSKGAKDETTTIFNKYLELHRTSESLDVQLIPVSVLWGRSPGQEDKSDLPNLRLLNGIQKTFAAIWFG
RDTFVRFSQAVSLRYMVVEHGSDEKIAQKLARVAKMHFAKQRISATGPRLPNRQAMFNKLLQSEAIRRAIEDEAKSKNIS
IEKAQKEAYKILDEIAADVSHSSLRAVDRFLRWLWNKLYSGINVQNSNRVRKLALEGHEIVYVPCHRSHIDYLLLSYVLY
HQGLVPPHIAAGINLNFWPIGRMFRSWGAFFIRRTFKGNRLYSAIFREYLSELFHRGYSVEYFIEGGRSRTGRLLAPKTG
MMSMTLQALQHSQTRPISIVPVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGQGFVNFGEPITLSNYLS
QHFPDWKEQNHEEKPQWFTPAVNNISKQVMININKAAAVNSMNLVGTALLSSRQRALSREQLLEQLSSYQQLLQNVPYST
DVVLPNVTPQAMLEHVLALDRIGVLIEKDNFGEIVRLERSSAVLMTYYRNNIQHLFVLPSLVASIILHYEAIQKDLLLDA
IRKIYPFLQGELFLHFNEDELNVQIHQIINEFARQSVINSNDNFLSINKSKVRILQLWSAGMLEILQRYYITVTILQKQP
AISRAELEKESQLVAQRLSVLHGINAPEFFDKAVFSSFIANLKEQRYFDESGYTVLDKIEELASTLSHLISTEICLTVKG
TIEKSEDLSS
>Mature_809_residues
ANFINMYRQLLSLPLSALVKNNPIPANPIEELSLNIHQPIVYVLPYTSQTDFVIFRRNCLALGLPDPAEKNEINGVKLPR
YVYLDEGRRIFKSKGAKDETTTIFNKYLELHRTSESLDVQLIPVSVLWGRSPGQEDKSDLPNLRLLNGIQKTFAAIWFGR
DTFVRFSQAVSLRYMVVEHGSDEKIAQKLARVAKMHFAKQRISATGPRLPNRQAMFNKLLQSEAIRRAIEDEAKSKNISI
EKAQKEAYKILDEIAADVSHSSLRAVDRFLRWLWNKLYSGINVQNSNRVRKLALEGHEIVYVPCHRSHIDYLLLSYVLYH
QGLVPPHIAAGINLNFWPIGRMFRSWGAFFIRRTFKGNRLYSAIFREYLSELFHRGYSVEYFIEGGRSRTGRLLAPKTGM
MSMTLQALQHSQTRPISIVPVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGQGFVNFGEPITLSNYLSQ
HFPDWKEQNHEEKPQWFTPAVNNISKQVMININKAAAVNSMNLVGTALLSSRQRALSREQLLEQLSSYQQLLQNVPYSTD
VVLPNVTPQAMLEHVLALDRIGVLIEKDNFGEIVRLERSSAVLMTYYRNNIQHLFVLPSLVASIILHYEAIQKDLLLDAI
RKIYPFLQGELFLHFNEDELNVQIHQIINEFARQSVINSNDNFLSINKSKVRILQLWSAGMLEILQRYYITVTILQKQPA
ISRAELEKESQLVAQRLSVLHGINAPEFFDKAVFSSFIANLKEQRYFDESGYTVLDKIEELASTLSHLISTEICLTVKGT
IEKSEDLSS

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=471, Percent_Identity=28.8747346072187, Blast_Score=179, Evalue=1e-44,
Organism=Homo sapiens, GI190358539, Length=433, Percent_Identity=24.7113163972286, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI87082362, Length=806, Percent_Identity=57.0719602977667, Blast_Score=951, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=283, Percent_Identity=31.8021201413428, Blast_Score=150, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71988723, Length=333, Percent_Identity=29.7297297297297, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71988728, Length=222, Percent_Identity=35.5855855855856, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17864692, Length=277, Percent_Identity=34.6570397111913, Blast_Score=166, Evalue=6e-41,
Organism=Drosophila melanogaster, GI21357731, Length=488, Percent_Identity=27.0491803278689, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24650754, Length=488, Percent_Identity=27.0491803278689, Blast_Score=136, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24650752, Length=488, Percent_Identity=27.0491803278689, Blast_Score=136, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 92776; Mature: 92645

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFINMYRQLLSLPLSALVKNNPIPANPIEELSLNIHQPIVYVLPYTSQTDFVIFRRNC
CCHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEECCE
LALGLPDPAEKNEINGVKLPRYVYLDEGRRIFKSKGAKDETTTIFNKYLELHRTSESLDV
EEECCCCCCCCCCCCCEECCEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEE
QLIPVSVLWGRSPGQEDKSDLPNLRLLNGIQKTFAAIWFGRDTFVRFSQAVSLRYMVVEH
EEEEEEEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEC
GSDEKIAQKLARVAKMHFAKQRISATGPRLPNRQAMFNKLLQSEAIRRAIEDEAKSKNIS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IEKAQKEAYKILDEIAADVSHSSLRAVDRFLRWLWNKLYSGINVQNSNRVRKLALEGHEI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCEE
VYVPCHRSHIDYLLLSYVLYHQGLVPPHIAAGINLNFWPIGRMFRSWGAFFIRRTFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCH
LYSAIFREYLSELFHRGYSVEYFIEGGRSRTGRLLAPKTGMMSMTLQALQHSQTRPISIV
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCCCCCCEEEE
PVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGQGFVNFGEPITLSNYLS
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCCCCEEHHHHHH
QHFPDWKEQNHEEKPQWFTPAVNNISKQVMININKAAAVNSMNLVGTALLSSRQRALSRE
HHCCCHHHCCCCCCCCCCCHHHHCCCEEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHH
QLLEQLSSYQQLLQNVPYSTDVVLPNVTPQAMLEHVLALDRIGVLIEKDNFGEIVRLERS
HHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCEEEECCCCCCEEEEECC
SAVLMTYYRNNIQHLFVLPSLVASIILHYEAIQKDLLLDAIRKIYPFLQGELFLHFNEDE
CEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
LNVQIHQIINEFARQSVINSNDNFLSINKSKVRILQLWSAGMLEILQRYYITVTILQKQP
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
AISRAELEKESQLVAQRLSVLHGINAPEFFDKAVFSSFIANLKEQRYFDESGYTVLDKIE
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ELASTLSHLISTEICLTVKGTIEKSEDLSS
HHHHHHHHHHHHHEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ANFINMYRQLLSLPLSALVKNNPIPANPIEELSLNIHQPIVYVLPYTSQTDFVIFRRNC
CHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEECCE
LALGLPDPAEKNEINGVKLPRYVYLDEGRRIFKSKGAKDETTTIFNKYLELHRTSESLDV
EEECCCCCCCCCCCCCEECCEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEE
QLIPVSVLWGRSPGQEDKSDLPNLRLLNGIQKTFAAIWFGRDTFVRFSQAVSLRYMVVEH
EEEEEEEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEC
GSDEKIAQKLARVAKMHFAKQRISATGPRLPNRQAMFNKLLQSEAIRRAIEDEAKSKNIS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IEKAQKEAYKILDEIAADVSHSSLRAVDRFLRWLWNKLYSGINVQNSNRVRKLALEGHEI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCEE
VYVPCHRSHIDYLLLSYVLYHQGLVPPHIAAGINLNFWPIGRMFRSWGAFFIRRTFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCH
LYSAIFREYLSELFHRGYSVEYFIEGGRSRTGRLLAPKTGMMSMTLQALQHSQTRPISIV
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCCCCCCEEEE
PVYVGYEHVLEVDTYAKELRGAAKEKENAGLVLRVIKKLRNLGQGFVNFGEPITLSNYLS
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCCCCEEHHHHHH
QHFPDWKEQNHEEKPQWFTPAVNNISKQVMININKAAAVNSMNLVGTALLSSRQRALSRE
HHCCCHHHCCCCCCCCCCCHHHHCCCEEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHH
QLLEQLSSYQQLLQNVPYSTDVVLPNVTPQAMLEHVLALDRIGVLIEKDNFGEIVRLERS
HHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCEEEECCCCCCEEEEECC
SAVLMTYYRNNIQHLFVLPSLVASIILHYEAIQKDLLLDAIRKIYPFLQGELFLHFNEDE
CEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
LNVQIHQIINEFARQSVINSNDNFLSINKSKVRILQLWSAGMLEILQRYYITVTILQKQP
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
AISRAELEKESQLVAQRLSVLHGINAPEFFDKAVFSSFIANLKEQRYFDESGYTVLDKIE
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ELASTLSHLISTEICLTVKGTIEKSEDLSS
HHHHHHHHHHHHHEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA