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The map label for this gene is rluE

Identifier: 16272636

GI number: 16272636

Start: 738386

End: 739108

Strand: Direct

Name: rluE

Synonym: HI0694

Alternate gene names: 16272636

Gene position: 738386-739108 (Clockwise)

Preceding gene: 16272635

Following gene: 16272642

Centisome position: 40.35

GC content: 37.48

Gene sequence:

>723_bases
ATGATGTTGCAAGCCTTTCAAAATAGAAATCATAAGTTAATGAAGAAAAATTTTTCAGCAGGGAAATTGCCGTCTAAAGG
AAAAAGTGCGGTTAATTTTCACCGCACTTTTAAGCCAAAATTAAAACCCAAAACGTTGTCGTTGGATGAAACCAAAGTTG
TGTTATTCAATAAACCTTTTGATGTACTCACGCAATTTACCGACGAACAAGGACGCGCTACCTTAAAAGATTTTATTTCA
ATTCCAAATGTTTATGCGGCAGGTCGATTAGATAGAGACAGTGAAGGATTACTGATTTTAACCAATAATGGCGAATTACA
ACATCGTTTGGCAGATCCCAAATTTAAAACAGAAAAAACCTATTGGGTGCAAGTGGAAGGTATCCCTGAAGAAACCGATT
TAGCACAACTGAGAAAAGGCGTTGAACTCAAAGATGGCGTGACAAAATCCGCTAAAGTGCGGTTAATTTCAGAGCCAAAT
TTATGGGAACGCAATCCACCTATCCGAGAACGGAAAAATATTCCCACAAGCTGGTTAGAAATTAAGATTAGTGAAGGGCG
CAACCGCCAAGTGCGGAGAATGACTGCGCATATTGGTTTCCCCACTTTACGATTAGTGAGAGTGAGTATGGGCTTATTAT
CTATTAATGGATTAGAAAATGGCTCATTTAGATTATTATCATTGGATGAAATAAAAGCGTTATTTCAGACCGTCAAATTA
TAA

Upstream 100 bases:

>100_bases
AATTTAATTGAGGGGCTAAGCTCAGTTTTTACTGGCTTAGCCTTTTTAATTTTTCAAGTTCACTCAAGTAATCATCATTA
CTACAGCCTGAAGTTTTGCT

Downstream 100 bases:

>100_bases
AAATAGGGCAAGTATTTAGCTTGCCCTAAATTTTTCTCCCATAAATATTGCGCACTGAGTAAATTAGCACTATCCGCAAC
GCGATGAGCTTGAGCTTGGT

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MMLQAFQNRNHKLMKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPFDVLTQFTDEQGRATLKDFIS
IPNVYAAGRLDRDSEGLLILTNNGELQHRLADPKFKTEKTYWVQVEGIPEETDLAQLRKGVELKDGVTKSAKVRLISEPN
LWERNPPIRERKNIPTSWLEIKISEGRNRQVRRMTAHIGFPTLRLVRVSMGLLSINGLENGSFRLLSLDEIKALFQTVKL

Sequences:

>Translated_240_residues
MMLQAFQNRNHKLMKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPFDVLTQFTDEQGRATLKDFIS
IPNVYAAGRLDRDSEGLLILTNNGELQHRLADPKFKTEKTYWVQVEGIPEETDLAQLRKGVELKDGVTKSAKVRLISEPN
LWERNPPIRERKNIPTSWLEIKISEGRNRQVRRMTAHIGFPTLRLVRVSMGLLSINGLENGSFRLLSLDEIKALFQTVKL
>Mature_240_residues
MMLQAFQNRNHKLMKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPFDVLTQFTDEQGRATLKDFIS
IPNVYAAGRLDRDSEGLLILTNNGELQHRLADPKFKTEKTYWVQVEGIPEETDLAQLRKGVELKDGVTKSAKVRLISEPN
LWERNPPIRERKNIPTSWLEIKISEGRNRQVRRMTAHIGFPTLRLVRVSMGLLSINGLENGSFRLLSLDEIKALFQTVKL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family

Homologues:

Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=66.2857142857143, Blast_Score=238, Evalue=2e-64,
Organism=Escherichia coli, GI1787524, Length=239, Percent_Identity=28.8702928870293, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788510, Length=186, Percent_Identity=31.7204301075269, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790453, Length=188, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUE_HAEIN (P44827)

Other databases:

- EMBL:   L42023
- PIR:   I64156
- RefSeq:   NP_438854.1
- ProteinModelPortal:   P44827
- SMR:   P44827
- GeneID:   950211
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0694
- NMPDR:   fig|71421.1.peg.664
- TIGR:   HI_0694
- HOGENOM:   HBG754478
- OMA:   CQFSPHE
- ProtClustDB:   CLSK892379
- BioCyc:   HINF71421:HI_0694-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 27464; Mature: 27464

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: PS01149 PSI_RSU

Important sites: ACT_SITE 91-91

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLQAFQNRNHKLMKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPF
CCCCCHHCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCEEEEEEECCH
DVLTQFTDEQGRATLKDFISIPNVYAAGRLDRDSEGLLILTNNGELQHRLADPKFKTEKT
HHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCE
YWVQVEGIPEETDLAQLRKGVELKDGVTKSAKVRLISEPNLWERNPPIRERKNIPTSWLE
EEEEEECCCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCCEEEE
IKISEGRNRQVRRMTAHIGFPTLRLVRVSMGLLSINGLENGSFRLLSLDEIKALFQTVKL
EEECCCCCHHHHHHHHHCCCCHHHHHHHHHCEEEECCCCCCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MMLQAFQNRNHKLMKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPF
CCCCCHHCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCEEEEEEECCH
DVLTQFTDEQGRATLKDFISIPNVYAAGRLDRDSEGLLILTNNGELQHRLADPKFKTEKT
HHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCE
YWVQVEGIPEETDLAQLRKGVELKDGVTKSAKVRLISEPNLWERNPPIRERKNIPTSWLE
EEEEEECCCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCCEEEE
IKISEGRNRQVRRMTAHIGFPTLRLVRVSMGLLSINGLENGSFRLLSLDEIKALFQTVKL
EEECCCCCHHHHHHHHHCCCCHHHHHHHHHCEEEECCCCCCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800