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The map label for this gene is ispD [H]

Identifier: 16272614

GI number: 16272614

Start: 715613

End: 716290

Strand: Reverse

Name: ispD [H]

Synonym: HI0672

Alternate gene names: 16272614

Gene position: 716290-715613 (Counterclockwise)

Preceding gene: 16272615

Following gene: 16272613

Centisome position: 39.14

GC content: 40.41

Gene sequence:

>678_bases
ATGGCTCGTTCAATTATTGCGGTCTTGCCAGCTGCTGGTGTTGGTTCACGAATGCAAGCGGATAAACCGAAACAATATTT
AACGCTGCTTGGCAAAACACTGCTTGAACATACACTCGATGTGATGCTTTCTTATCCTGCCGTATCTAAAATTATCTTGG
CGGTAAGCAAAGATGATCCTTATATTTCTACACTATCGCTCGATCCTAAAATTCAGTTAGTGGAAGGTGGCACAACGCGA
GCTGAATCGGTCTTAAATGGTTTAAATGCAATTGCGGAAAAAAATGCCTGGGTGCTTGTTCATGATGCTGCTCGCCCTTG
TTTACAACATGCTGATATTGATAAATTGTTAGCTATTGAAGATAAACAAGGTGCGATTTTGGCAATCCCAGTAACAGATA
CGATTAAACGAGCGGACAATCAGCAATGTATTGTAAAAACAGAAGATCGTAGCCAGCTTTGGCAAGCAATGACACCGCAG
TTTTTCCCAGTAGATATATTAAGAGATGCGCTTTCCACAGGAATTCAACAAGGTGCGAATATAACAGATGAAGCATCAGC
AATAGAACTTGCTGGATTTCGACCGCACTTAGTGGCTGGACGTAGCGATAATTTAAAAGTCACTCGTCCTGAAGATTTAG
CATTAGCAGAATTTTATTTAACAAGGAATAAATTATGA

Upstream 100 bases:

>100_bases
TAACAAAAGGTTTTGAAGCGATCGAAGAACGCGCACGAATGCAACATGGCTTAGTGAAAGAAAATGAAGTGTTCTATCAT
ATTGTAAAAGAGAGTAAATA

Downstream 100 bases:

>100_bases
TCAGAATTGGACACGGCTTTGATGTTCACGCTTTTGGCGAAGATCGTCCATTAATTATTGGTGGTGTAGAAGTGCCTTAT
CATACGGGCTTTATTGCGCA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MARSIIAVLPAAGVGSRMQADKPKQYLTLLGKTLLEHTLDVMLSYPAVSKIILAVSKDDPYISTLSLDPKIQLVEGGTTR
AESVLNGLNAIAEKNAWVLVHDAARPCLQHADIDKLLAIEDKQGAILAIPVTDTIKRADNQQCIVKTEDRSQLWQAMTPQ
FFPVDILRDALSTGIQQGANITDEASAIELAGFRPHLVAGRSDNLKVTRPEDLALAEFYLTRNKL

Sequences:

>Translated_225_residues
MARSIIAVLPAAGVGSRMQADKPKQYLTLLGKTLLEHTLDVMLSYPAVSKIILAVSKDDPYISTLSLDPKIQLVEGGTTR
AESVLNGLNAIAEKNAWVLVHDAARPCLQHADIDKLLAIEDKQGAILAIPVTDTIKRADNQQCIVKTEDRSQLWQAMTPQ
FFPVDILRDALSTGIQQGANITDEASAIELAGFRPHLVAGRSDNLKVTRPEDLALAEFYLTRNKL
>Mature_224_residues
ARSIIAVLPAAGVGSRMQADKPKQYLTLLGKTLLEHTLDVMLSYPAVSKIILAVSKDDPYISTLSLDPKIQLVEGGTTRA
ESVLNGLNAIAEKNAWVLVHDAARPCLQHADIDKLLAIEDKQGAILAIPVTDTIKRADNQQCIVKTEDRSQLWQAMTPQF
FPVDILRDALSTGIQQGANITDEASAIELAGFRPHLVAGRSDNLKVTRPEDLALAEFYLTRNKL

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=230, Percent_Identity=29.1304347826087, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI157671913, Length=135, Percent_Identity=31.1111111111111, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=53.3632286995516, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 24549; Mature: 24417

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSIIAVLPAAGVGSRMQADKPKQYLTLLGKTLLEHTLDVMLSYPAVSKIILAVSKDDP
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCC
YISTLSLDPKIQLVEGGTTRAESVLNGLNAIAEKNAWVLVHDAARPCLQHADIDKLLAIE
EEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCHHEEEEE
DKQGAILAIPVTDTIKRADNQQCIVKTEDRSQLWQAMTPQFFPVDILRDALSTGIQQGAN
CCCCCEEEEECHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
ITDEASAIELAGFRPHLVAGRSDNLKVTRPEDLALAEFYLTRNKL
CCCCCCEEEECCCCCEEEECCCCCEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ARSIIAVLPAAGVGSRMQADKPKQYLTLLGKTLLEHTLDVMLSYPAVSKIILAVSKDDP
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCC
YISTLSLDPKIQLVEGGTTRAESVLNGLNAIAEKNAWVLVHDAARPCLQHADIDKLLAIE
EEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCHHEEEEE
DKQGAILAIPVTDTIKRADNQQCIVKTEDRSQLWQAMTPQFFPVDILRDALSTGIQQGAN
CCCCCEEEEECHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
ITDEASAIELAGFRPHLVAGRSDNLKVTRPEDLALAEFYLTRNKL
CCCCCCEEEECCCCCEEEECCCCCEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA