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The map label for this gene is arcB

Identifier: 16272539

GI number: 16272539

Start: 618348

End: 619352

Strand: Reverse

Name: arcB

Synonym: HI0596

Alternate gene names: 16272539

Gene position: 619352-618348 (Counterclockwise)

Preceding gene: 161350026

Following gene: 16272538

Centisome position: 33.84

GC content: 38.91

Gene sequence:

>1005_bases
ATGGCTTTCAATATGAAAAATAGACATTTACTTAGTCTTGTTCATCACACAGAACGTGAAATTAAATATCTCTTAGATCT
CTCCCGAGATTTGAAACGAGCCAAATATGCTGGCACAGAACAACAAAAATTAAAAGGCAAAAATATCGCGCTCATCTTTG
AAAAAACCTCCACCCGTACACGCTGTGCATTTGAAGTCGCCGCTTATGATCAAGGCGCACAAGTCACCTACATCGACCCT
AATTCCTCTCAAATTGGCCACAAAGAAAGCATGAAAGATACCGCCCGCGTATTAGGCAGAATGTATGATGGCATTGAATA
TCGTGGCTTTAAACAAAGTATCGTTCAAGAACTGGCTGATTATGCTGGAGTGCCAGTATTCAATGGTTTAACCGATGAAT
TCCACCCAACACAAATGCTTGCCGACGTACTTACCATGATTGAACATTGCGACAAACCATTAAGTGAAATTAGCTATGTA
TATATTGGGGATGCTCGCAATAACATGGGTAATTCACTTTTATTAATTGGTGCAAAATTAGGTATGGATGTTCGTATTTG
TGGACCTAAAGCATTATTACCAGAAGCAAATCTTGTGGAAATGTGCGAAAAATTTGCGAAAGAAAGTGGAGCTCGTATTA
CTGTAACTGAAGATATCGACAAAGCGGTGAAAGGCGTAGATTTTATCCATACTGATGTGTGGGTTTCAATGGGTGAACCA
TTAGAAACTTGGGGCGAACGCATTAAATTATTACTTCCTTATCAAGTCACACCTGAATTAATGAAACGCACTGGTAATCC
AAAAGTGAAATTTATGCACTGCTTGCCAGCCTTCCATAACAGCGAAACCAAAGTTGGTCGTCAAATTGCTGAAAAATATC
CTGAATTAGCTAATGGTATTGAAGTAACTGAAGAGGTATTTGAATCGCCAATGAACATTGCATTTGAACAAGCAGAAAAC
CGTATGCACACAATTAAAGCGGTAATGGTAGCAAGTTTGGCATAA

Upstream 100 bases:

>100_bases
ATTTTACCTCTCTTATAATGCAACTGTTCGAACAACAAGCAGTATCTCGAATGATGTATGTTTATTCCCCAATGTATAGT
CAAAACACAAGGAGATTATT

Downstream 100 bases:

>100_bases
GGAGAACGTATGAGAATAGTGATTGCATTAGGCGGTAATGCGTTATTACGACGCGGAGAACCAATGACAGCCGAAAATCA
ACGACAAAATGTTCGCATTG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRTRCAFEVAAYDQGAQVTYIDP
NSSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYV
YIGDARNNMGNSLLLIGAKLGMDVRICGPKALLPEANLVEMCEKFAKESGARITVTEDIDKAVKGVDFIHTDVWVSMGEP
LETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGIEVTEEVFESPMNIAFEQAEN
RMHTIKAVMVASLA

Sequences:

>Translated_334_residues
MAFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRTRCAFEVAAYDQGAQVTYIDP
NSSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYV
YIGDARNNMGNSLLLIGAKLGMDVRICGPKALLPEANLVEMCEKFAKESGARITVTEDIDKAVKGVDFIHTDVWVSMGEP
LETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGIEVTEEVFESPMNIAFEQAEN
RMHTIKAVMVASLA
>Mature_333_residues
AFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRTRCAFEVAAYDQGAQVTYIDPN
SSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYVY
IGDARNNMGNSLLLIGAKLGMDVRICGPKALLPEANLVEMCEKFAKESGARITVTEDIDKAVKGVDFIHTDVWVSMGEPL
ETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGIEVTEEVFESPMNIAFEQAENR
MHTIKAVMVASLA

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=330, Percent_Identity=39.0909090909091, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI18105007, Length=338, Percent_Identity=23.0769230769231, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=62.0060790273556, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1786469, Length=332, Percent_Identity=60.2409638554217, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI48994908, Length=383, Percent_Identity=22.976501305483, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI2367364, Length=320, Percent_Identity=26.25, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322373, Length=330, Percent_Identity=32.4242424242424, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322331, Length=195, Percent_Identity=27.1794871794872, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24642586, Length=333, Percent_Identity=22.2222222222222, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTCC_HAEIN (P44770)

Other databases:

- EMBL:   L42023
- PIR:   H64079
- RefSeq:   NP_438753.1
- ProteinModelPortal:   P44770
- SMR:   P44770
- GeneID:   950778
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0596
- TIGR:   HI_0596
- HOGENOM:   HBG579429
- OMA:   MTPYQIN
- ProtClustDB:   PRK01713
- BioCyc:   HINF71421:HI_0596-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 37648; Mature: 37517

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 108-108 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCC
RCAFEVAAYDQGAQVTYIDPNSSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELAD
CEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
YAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYVYIGDARNNMGNSLLLIGAKL
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCCEEEEEEECC
GMDVRICGPKALLPEANLVEMCEKFAKESGARITVTEDIDKAVKGVDFIHTDVWVSMGEP
CCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHCCCCC
LETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGI
HHHHHHHEEEEECCCCCHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCHH
EVTEEVFESPMNIAFEQAENRMHTIKAVMVASLA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCC
RCAFEVAAYDQGAQVTYIDPNSSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELAD
CEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
YAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYVYIGDARNNMGNSLLLIGAKL
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCCEEEEEEECC
GMDVRICGPKALLPEANLVEMCEKFAKESGARITVTEDIDKAVKGVDFIHTDVWVSMGEP
CCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHCCCCC
LETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGI
HHHHHHHEEEEECCCCCHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCHH
EVTEEVFESPMNIAFEQAENRMHTIKAVMVASLA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800