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The map label for this gene is gidA [H]

Identifier: 16272526

GI number: 16272526

Start: 601115

End: 603004

Strand: Reverse

Name: gidA [H]

Synonym: HI0582

Alternate gene names: 16272526

Gene position: 603004-601115 (Counterclockwise)

Preceding gene: 16272527

Following gene: 16272525

Centisome position: 32.95

GC content: 40.53

Gene sequence:

>1890_bases
ATGTTTTACACTGAAACTTATGATGTGATTGTGATCGGTGGTGGTCATGCGGGTACAGAAGCCGCACTTGCACCAGCTCG
TATGGGATTTAAAACCCTTTTATTAACACATAATGTAGATACTTTAGGGCAAATGTCTTGTAACCCTGCAATTGGTGGGA
TCGGTAAAGGTCATTTAGTAAAAGAAGTAGATGCAATGGGCGGTTTAATGGCGCATGCTGCAGATAAAGCAGGGATCCAA
TTTCGTACTTTAAATAGCAGTAAAGGCCCAGCAGTGCGTGCTACTCGAGCTCAATCTGACAGAGTTCTATATCGTCAAGC
TGTTCGTACTGCATTAGAAAATCAACCTAATTTAGATATTTTCCAACAAGAAGCGACAGATATTCTGATTGAGCAAGATC
GAGTTACAGGCGTTAGCACAAAAATGGGATTAACCTTCCGTGCAAAATCAGTGATCTTAACGGCCGGTACTTTCTTAGCT
GGTAAAATTCATATTGGTTTGGAAAATTATGAAGGTGGCCGTGCAGGAGATTCTGCTTCTGTAAATCTTTCACATCGATT
AAGAGATCTCGGATTACGTGTAGATCGCCTTAAAACAGGTACACCGCCGCGTATTGATGCGCGTACGATCAATTTTGATA
TTTTAGCTAAACAACATGGTGATGAGGTGTTGCCCGTGTTCTCTTTTATGGGATCTGTTGATGATCACCCTCAACAAATT
CCTTGTTATATAACCCATACCAATGAACAAACCCATGAAGTGATCCGTAATAACTTGGATCGCAGTCCAATGTATACCGG
TGTGATTGAAGGGATTGGTCCACGTTATTGCCCATCCATTGAAGATAAAGTGATGCGTTTCGCGGATCGTAATTCACATC
AAATTTATTTAGAACCAGAAGGCTTAACCAGTAATGAAGTGTATCCAAACGGGATCTCTACCAGTTTACCGTTTGACGTG
CAAATGGGCATTGTGAATTCTATGAAAGGTTTAGAAAACGCACGTATTGTGAAACCAGGTTATGCTATTGAATACGATTA
TTTTGATCCACGTGACTTAAAACCAACCTTAGAAACTAAATCAATTTCTGGTCTATTTTTCGCAGGTCAAATTAACGGTA
CAACTGGTTATGAAGAAGCAGCGGCACAAGGTTTATTGGCTGGGATTAACGCAGGTCTTTATGTTCAAGAGAAGGATGCA
TGGTATCCACGCCGTGATCAATCTTATACTGGCGTATTGGTGGATGACCTTTGCACACTTGGTACCAAAGAACCGTATCG
CGTATTTACTTCTCGTGCAGAATACCGTTTGTTACTACGTGAAGACAATGCAGACATTCGTTTAACACCAATTGCCCATG
AATTAGGTTTGATTGATGAGGCTCGCTGGGCACGTTTCAATCAAAAAATGGAAAATATTGAACAGGAACGCCAACGTTTA
CGCAGCATTTGGTTGCATCCTCGTTCGGAATATTTAGAAGAAGCGAATAAAGTGCTTGGTAGTCCACTTGTACGTGAGGC
TAGCGGTGAAGATTTATTGCGTCGTCCTGAAATGACCTACGATATTTTGACTTCTTTAACGCCATATAAACCGGCAATGG
AAGATAAGGAAGCTGTTGAGCAAGTAGAAATTGCTATTAAATATCAAGGCTATATCGAGCATCAACAAGAAGAAATCGAA
AAACAAAAACGCCATGAAAATACGGCTATTCCAGCTAACTTTGATTATAGTAAAGTTTCCGGTTTATCTAATGAAGTACG
TGCGAAATTGGAACAACATCGTCCGGTTTCCATTGGTCAAGCGAGCCGAATTTCAGGTATCACCCCTGCAGCAATTTCAA
TTATTTTGGTAAATTTGAAAAAACAAGGAATGCTAAAACGTGGGGAATAA

Upstream 100 bases:

>100_bases
GATCTTTCAAAGGATCAATAGATCCATTCTTTTTTTGTCAGGTGTTTTAAATTTCTGTAGAATAACGCCAATTTTTTGAT
CAGAATAAATAGAGATAGAT

Downstream 100 bases:

>100_bases
AGTTTTTTATAAAAAAACAAAAGTGCGGTAAATTTTAATCTAGTTTTAATCCTAGAGCATATTGTTTAATTATTGACAAT
AAAGCGTAAAACTATTAGAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVKEVDAMGGLMAHAADKAGIQ
FRTLNSSKGPAVRATRAQSDRVLYRQAVRTALENQPNLDIFQQEATDILIEQDRVTGVSTKMGLTFRAKSVILTAGTFLA
GKIHIGLENYEGGRAGDSASVNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPVFSFMGSVDDHPQQI
PCYITHTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFADRNSHQIYLEPEGLTSNEVYPNGISTSLPFDV
QMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETKSISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDA
WYPRRDQSYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDEARWARFNQKMENIEQERQRL
RSIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVEQVEIAIKYQGYIEHQQEEIE
KQKRHENTAIPANFDYSKVSGLSNEVRAKLEQHRPVSIGQASRISGITPAAISIILVNLKKQGMLKRGE

Sequences:

>Translated_629_residues
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVKEVDAMGGLMAHAADKAGIQ
FRTLNSSKGPAVRATRAQSDRVLYRQAVRTALENQPNLDIFQQEATDILIEQDRVTGVSTKMGLTFRAKSVILTAGTFLA
GKIHIGLENYEGGRAGDSASVNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPVFSFMGSVDDHPQQI
PCYITHTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFADRNSHQIYLEPEGLTSNEVYPNGISTSLPFDV
QMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETKSISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDA
WYPRRDQSYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDEARWARFNQKMENIEQERQRL
RSIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVEQVEIAIKYQGYIEHQQEEIE
KQKRHENTAIPANFDYSKVSGLSNEVRAKLEQHRPVSIGQASRISGITPAAISIILVNLKKQGMLKRGE
>Mature_629_residues
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVKEVDAMGGLMAHAADKAGIQ
FRTLNSSKGPAVRATRAQSDRVLYRQAVRTALENQPNLDIFQQEATDILIEQDRVTGVSTKMGLTFRAKSVILTAGTFLA
GKIHIGLENYEGGRAGDSASVNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPVFSFMGSVDDHPQQI
PCYITHTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFADRNSHQIYLEPEGLTSNEVYPNGISTSLPFDV
QMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETKSISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDA
WYPRRDQSYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDEARWARFNQKMENIEQERQRL
RSIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVEQVEIAIKYQGYIEHQQEEIE
KQKRHENTAIPANFDYSKVSGLSNEVRAKLEQHRPVSIGQASRISGITPAAISIILVNLKKQGMLKRGE

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=643, Percent_Identity=46.656298600311, Blast_Score=518, Evalue=1e-147,
Organism=Homo sapiens, GI19882217, Length=668, Percent_Identity=44.9101796407186, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI183227703, Length=683, Percent_Identity=43.9238653001464, Blast_Score=498, Evalue=1e-141,
Organism=Escherichia coli, GI2367273, Length=627, Percent_Identity=76.3955342902711, Blast_Score=1015, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=41.3183279742765, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321202, Length=626, Percent_Identity=43.7699680511182, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24658174, Length=637, Percent_Identity=46.3108320251177, Blast_Score=521, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70104; Mature: 70104

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLV
CCEEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCCCCCHHH
KEVDAMGGLMAHAADKAGIQFRTLNSSKGPAVRATRAQSDRVLYRQAVRTALENQPNLDI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCH
FQQEATDILIEQDRVTGVSTKMGLTFRAKSVILTAGTFLAGKIHIGLENYEGGRAGDSAS
HHHCCHHEEEECCCCCCCEECCCEEEEECEEEEEECCEEEEEEEEEEECCCCCCCCCCCE
VNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPVFSFMGSVDDHPQQI
EEHHHHHHHHCCEEEHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCC
PCYITHTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFADRNSHQIYLEPE
CEEEECCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEVYPNGISTSLPFDVQMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETK
CCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCCCCCCC
SISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDAWYPRRDQSYTGVLVDDLCTL
CCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHC
GTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDEARWARFNQKMENIEQERQRL
CCCCCHHHHHCCCEEEEEEEECCCCEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RSIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVE
HHHHCCCHHHHHHHHHHHHCCCHHHCCCCCHHHHCCCCHHHHHHCCCCCCCCCCHHHHHH
QVEIAIKYQGYIEHQQEEIEKQKRHENTAIPANFDYSKVSGLSNEVRAKLEQHRPVSIGQ
HEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCC
ASRISGITPAAISIILVNLKKQGMLKRGE
CCCCCCCCHHHEEEEEEECHHCCCCCCCC
>Mature Secondary Structure
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLV
CCEEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCCCCCHHH
KEVDAMGGLMAHAADKAGIQFRTLNSSKGPAVRATRAQSDRVLYRQAVRTALENQPNLDI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCH
FQQEATDILIEQDRVTGVSTKMGLTFRAKSVILTAGTFLAGKIHIGLENYEGGRAGDSAS
HHHCCHHEEEECCCCCCCEECCCEEEEECEEEEEECCEEEEEEEEEEECCCCCCCCCCCE
VNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPVFSFMGSVDDHPQQI
EEHHHHHHHHCCEEEHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCC
PCYITHTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFADRNSHQIYLEPE
CEEEECCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEVYPNGISTSLPFDVQMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETK
CCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCCCCCCC
SISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDAWYPRRDQSYTGVLVDDLCTL
CCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHC
GTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDEARWARFNQKMENIEQERQRL
CCCCCHHHHHCCCEEEEEEEECCCCEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RSIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVE
HHHHCCCHHHHHHHHHHHHCCCHHHCCCCCHHHHCCCCHHHHHHCCCCCCCCCCHHHHHH
QVEIAIKYQGYIEHQQEEIEKQKRHENTAIPANFDYSKVSGLSNEVRAKLEQHRPVSIGQ
HEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCC
ASRISGITPAAISIILVNLKKQGMLKRGE
CCCCCCCCHHHEEEEEEECHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA