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The map label for this gene is aspA

Identifier: 16272478

GI number: 16272478

Start: 558436

End: 559863

Strand: Reverse

Name: aspA

Synonym: HI0534

Alternate gene names: 16272478

Gene position: 559863-558436 (Counterclockwise)

Preceding gene: 16272479

Following gene: 16272474

Centisome position: 30.59

GC content: 37.82

Gene sequence:

>1428_bases
GTGATTACAATGACTCAATTTAGAAAAGAAGTAGATTTACTTGGCGAACGTGATGTGCCTGCAGAAGCATATTGGGGGAT
TCATACATTAAGAGCGGTAGAAAATTTCAATATTTCTAACGTAACCATTTCTGACGTGCCTGAGTTTGTGCGTGGTATGG
TAATGGTAAAAAAAGCAACAGCTTTAGCCAATGGCGAATTAGGTGCAATTCCAAGTGATATTGCAAAAGCGATTGTAGCA
GCTTGTGATGAAATTCTTACCACTGGAAAATGCTTAGATCAATTCCCATCAGACGTATATCAAGGTGGTGCAGGTACGTC
TGTCAATATGAATACTAATGAAGTGGTTGCTAATCTTGCCCTTGAAAAAATTGGTCATAAAAAAGGCGAATATAACGTGA
TTAATCCGATGGATCACGTTAATGCAAGCCAATCGACCAACGATGCGTATCCTACCGGTTTCCGTATTGCGGTGTACAAC
AGCATCTTAAAATTGATCGATAAAATTCAATATTTACACGACAGTTTTGATAATAAAGCAAAAGAGTTTGCAAATATCTT
AAAAATGGGTCGTACCCAATTGCAAGATGCGGTGCCAATGACTGTTGGTCAAGAATTCAAAGCTTTCGCCGTATTACTTG
AAGAAGAAGTGCGTAACTTAAAACGTACAGCAGGTTTATTACTTGAAGTAAACCTTGGTGCAACGGCAATCGGTACTGGT
TTAAATACGCCACAAGGCTATACAGAATTAGTTGTAAAACATCTTGCTGAAGTGACTGGATTAGCTTGTGTACCAGCAGA
AAACTTAATTGAAGCAACATCTGACTGTGGTGCTTATGTCATGGTTCACGGTGCATTAAAACGTACTGCAGTGAAACTTT
CTAAAGTATGTAATGACTTACGTTTACTTTCTTCTGGCCCACGTGCAGGTTTAAAAGAAATTAATCTTCCTGAATTACAA
GCGGGTTCTTCTATTATGCCAGCAAAAGTAAACCCAGTTGTTCCAGAGGTGGTAAACCAAGTGTGCTTTAAAGTAATTGG
TAACGATACCACTGTGACCTTCGCATCTGAAGCAGGTCAATTACAATTAAACGTAATGGAACCAGTGATTGGTCAAGCAA
TGTTTGAATCTATCGATATCTTAACCAATGCTTGTGTGAACTTACGCGATAAATGTGTGGATGGCATCACTGTAAACAAA
GAAATTTGTGAAAACTACGTGTTTAATTCAATTGGTATTGTGACTTACTTGAATCCATTTATCGGTCACCACAACGGCGA
CTTAGTGGGTAAAATCTGTGCACAAACAGGCAAAGGCGTACGTGAAGTTGTGTTAGAAAAAGGTTTATTAACAGAAGAAC
AATTAGATGACATTCTTTCTGTAGAAAACTTAATGAATCCAACTTACAAAGCGAAATTAAATAAATAA

Upstream 100 bases:

>100_bases
TGAAAATATGTCTTGAGAGCATTTTTTGAAAATGTGATCTTCATCAAGTTTTTTCTTCTTGAAATTTCTATAATGACGAT
AAATTAACTAACCCAAAAAG

Downstream 100 bases:

>100_bases
TTTACGCCAAATATCAAAAAAATTTACCGCACTTTAATCTGTGCCTTAAAGCTGAATAAATAAACCAGCTAATTGGGGTG
CAGATTTTTTTATGATTAAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MITMTQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALANGELGAIPSDIAKAIVA
ACDEILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEKIGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYN
SILKLIDKIQYLHDSFDNKAKEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTG
LNTPQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLKEINLPELQ
AGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNK
EICENYVFNSIGIVTYLNPFIGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK

Sequences:

>Translated_475_residues
MITMTQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALANGELGAIPSDIAKAIVA
ACDEILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEKIGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYN
SILKLIDKIQYLHDSFDNKAKEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTG
LNTPQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLKEINLPELQ
AGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNK
EICENYVFNSIGIVTYLNPFIGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK
>Mature_475_residues
MITMTQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALANGELGAIPSDIAKAIVA
ACDEILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEKIGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYN
SILKLIDKIQYLHDSFDNKAKEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTG
LNTPQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLKEINLPELQ
AGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNK
EICENYVFNSIGIVTYLNPFIGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily

Homologues:

Organism=Homo sapiens, GI19743875, Length=468, Percent_Identity=40.1709401709402, Blast_Score=335, Evalue=5e-92,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=78.5407725321888, Blast_Score=753, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=37.3650107991361, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1790398, Length=282, Percent_Identity=23.0496453900709, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=467, Percent_Identity=40.4710920770878, Blast_Score=351, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=41.1042944785276, Blast_Score=258, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=40.0862068965517, Blast_Score=337, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24640177, Length=465, Percent_Identity=40, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24640179, Length=465, Percent_Identity=40, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=39.2241379310345, Blast_Score=330, Evalue=9e-91,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=38.7931034482759, Blast_Score=330, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24583245, Length=449, Percent_Identity=32.739420935412, Blast_Score=266, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASPA_HAEIN (P44324)

Other databases:

- EMBL:   L42023
- PIR:   C64075
- RefSeq:   NP_438692.1
- ProteinModelPortal:   P44324
- SMR:   P44324
- GeneID:   949597
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0534
- NMPDR:   fig|71421.1.peg.505
- TIGR:   HI_0534
- HOGENOM:   HBG284369
- OMA:   VGKICAQ
- ProtClustDB:   PRK12273
- BioCyc:   HINF71421:HI_0534-MONOMER
- BRENDA:   4.3.1.1
- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00839

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.1.1

Molecular weight: Translated: 51484; Mature: 51484

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMTQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKAT
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHH
ALANGELGAIPSDIAKAIVAACDEILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHHHHHHHHH
LEKIGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYNSILKLIDKIQYLHDSFDNKA
HHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEECCC
LNTPQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDL
CCCCCCHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHH
RLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
LQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEICENYVFNSIGIVTYLNPF
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK
HCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCC
>Mature Secondary Structure
MITMTQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKAT
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHH
ALANGELGAIPSDIAKAIVAACDEILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHHHHHHHHH
LEKIGHKKGEYNVINPMDHVNASQSTNDAYPTGFRIAVYNSILKLIDKIQYLHDSFDNKA
HHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEECCC
LNTPQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDL
CCCCCCHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHH
RLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
LQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEICENYVFNSIGIVTYLNPF
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK
HCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800