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The map label for this gene is dnaG

Identifier: 16272476

GI number: 16272476

Start: 554392

End: 556173

Strand: Direct

Name: dnaG

Synonym: HI0532

Alternate gene names: 16272476

Gene position: 554392-556173 (Clockwise)

Preceding gene: 16272475

Following gene: 16272477

Centisome position: 30.29

GC content: 39.84

Gene sequence:

>1782_bases
ATGAAAGGTTCTATTCCTCGCCCCTTTATTGATGATTTGCTGACAAAGTCCGATATTGTCGATGTGATTAACACGCGCGT
AAAACTAAAAAAAGCTGGCCGCGATTATCAAGCCTGCTGCCCTTTCCATCACGAAAAAACACCATCCTTCACAGTTAGCC
AAAAGAAACAGTTTTATCATTGCTTTGGTTGTGGCGCACATGGTAATGCGATTTCCTTTTTAATGGATTATGACAAACTT
GAATTTATAGAGGCGATTGAAGAACTTGCAGCAATGGCAGGGCTTGAAATACCTTACGAAAAACGCGTTAATCACAGCGG
AAAACCTCAAGCTAATTACCAAACCAAACGAAATCTCTATGAATTAATGCAAGAGATTGCCACGTTTTATCAAAACCAAT
TACCATTAAATACTCAAGCACAAGAATATTTACAACAACGTGGGCTTTCACCTGAAATTATTGAGCGTTTCCAAATTGGG
TTTGTGCCGAATGCAATGGATACTGTACTGCGTAAATTTGGTGTTAATCGTGAAGAACAACAAAAACTCATTGAGTTAGG
CATGCTTTCTCGAAATGATCGTGGCAATATTTACGATAAATTCCGAAATCGCATCATGTTTCCAATCCGCGATAAACGTG
GTCGCACAGTGGCTTTTGGTGGGCGTGTATTAACAGATGAAAAACCGAAATATCTGAATTCGCCAGAAACCATTACTTAT
CATAAAGGTAAGGAGCTTTATGGTTTGTATGAAGCCTTGCAAACCAACGATGAACCCAAACAATTACTTGTTGTTGAAGG
TTATATGGATGTAGTGGCATTAGCACAATTTGGCGTGGATTATGCGGTCGCTTCTTTAGGGACATCCACGACATCAGAAC
AAATTCAACTTATTTTGCGTTCGACCGAACAGGTTGTTTGTTGTTACGATGGTGACCGTGCAGGGCGTGATGCTGCTTGG
CGCGCACTAGAAAATGCGTTGCCCTATTTAGAAGATGGCAGACAACTCAAATTTATTTTCTTGCCAGATGGAGAAGAACC
TGATACTTACATTCGTCAATACGGCAAAGAAAAATTTGAAGAATACATCGAAAGTGCGCAATCTTTAAGCGAGTTTATGT
TTGCACATTTAAGTCCTCAGGTTGATTTTTCCACTAAAGAAGGGCGTGGAAAATTAGTTGCGCTAGCTGCGCCGTTAATT
CACCAAATTCCAGGAGAAATGCTTCGCTTATCGCTACGCAATATGCTTGCTCAAAAACTTGGGATTTTTGATCAAACTCA
GCTGGAAAATCTTATTCCCAAAAAATTAGAACAAGCCAATACACAACAAAAAGTCACTCATAATAAGATCAAGAAGACAC
CGATGCGAATGGTCATTTCATTGCTTATGCAAAATCCTGAGTTAGTAAAACGTATGTCTGAAAGCGGCGTGCAAGCATTA
CGTGCGGAAGCAGGGTTTGAAATTCTGGAAAAATTGACCGCACTTTGTCGCCAACGAGAGGGCATTACTACGGGGCAAAT
TTTGGAATACTTCCGCAATACATCTTACAGTAATCCCCTTGAAATACTGGCGACTTGGGATCATTTATTGGACGAATCCG
ACATAATCAACGCATTTTCTCAAAATTACCGTCGCTTAAATATTCAAGCTATTGAGCGCGATATTGAAATGCTTATTGCT
AAAGAACGAACAGAGGGTCTCACTAATGAAGAAAAAACGGTACTTGTACATCTTTTAGCTGGTAAAGAACAGCAGAAAAA
ACAGTTAGTGAATCCGCTATAA

Upstream 100 bases:

>100_bases
CCGTGAATCCTTTGGACTCACGGTTTTGTTACTTTAAGCACAACAAAAATCTACGCCAAAAACGACCGCACTTTCACACC
ACGATCACGGAGGCTCGACA

Downstream 100 bases:

>100_bases
CAATGGTAGAATCTCAAGTTCATAAAAAATCTTATCTTCTCAATCACATCAAGCAAAACGAGTATAAAAATGGAAAAAAA
TCAACAATCTACGGCTGAAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMDYDKL
EFIEAIEELAAMAGLEIPYEKRVNHSGKPQANYQTKRNLYELMQEIATFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIG
FVPNAMDTVLRKFGVNREEQQKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLILRSTEQVVCCYDGDRAGRDAAW
RALENALPYLEDGRQLKFIFLPDGEEPDTYIRQYGKEKFEEYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLI
HQIPGEMLRLSLRNMLAQKLGIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLMQNPELVKRMSESGVQAL
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFSQNYRRLNIQAIERDIEMLIA
KERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL

Sequences:

>Translated_593_residues
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMDYDKL
EFIEAIEELAAMAGLEIPYEKRVNHSGKPQANYQTKRNLYELMQEIATFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIG
FVPNAMDTVLRKFGVNREEQQKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLILRSTEQVVCCYDGDRAGRDAAW
RALENALPYLEDGRQLKFIFLPDGEEPDTYIRQYGKEKFEEYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLI
HQIPGEMLRLSLRNMLAQKLGIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLMQNPELVKRMSESGVQAL
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFSQNYRRLNIQAIERDIEMLIA
KERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL
>Mature_593_residues
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMDYDKL
EFIEAIEELAAMAGLEIPYEKRVNHSGKPQANYQTKRNLYELMQEIATFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIG
FVPNAMDTVLRKFGVNREEQQKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLILRSTEQVVCCYDGDRAGRDAAW
RALENALPYLEDGRQLKFIFLPDGEEPDTYIRQYGKEKFEEYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLI
HQIPGEMLRLSLRNMLAQKLGIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLMQNPELVKRMSESGVQAL
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFSQNYRRLNIQAIERDIEMLIA
KERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=580, Percent_Identity=56.0344827586207, Blast_Score=671, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_HAEIN (Q08346)

Other databases:

- EMBL:   L11044
- EMBL:   L42023
- EMBL:   L01756
- PIR:   A64075
- RefSeq:   NP_438690.1
- ProteinModelPortal:   Q08346
- GeneID:   949627
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0532
- NMPDR:   fig|71421.1.peg.503
- TIGR:   HI_0532
- HOGENOM:   HBG573408
- OMA:   ALAEKEW
- ProtClustDB:   PRK05667
- BioCyc:   HINF71421:HI_0532-MONOMER
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:1.10.860.10
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00766
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 68002; Mature: 68002

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHH
CFGCGAHGNAISFLMDYDKLEFIEAIEELAAMAGLEIPYEKRVNHSGKPQANYQTKRNLY
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHH
ELMQEIATFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIGFVPNAMDTVLRKFGVNREEQ
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
QKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HHHHHHHHHCCCCCCCHHHHHHCCEECEEECCCCCEEEECCEEECCCCCCCCCCCCEEEE
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLILR
ECCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
STEQVVCCYDGDRAGRDAAWRALENALPYLEDGRQLKFIFLPDGEEPDTYIRQYGKEKFE
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
EYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLIHQIPGEMLRLSLRNMLAQKL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHH
GIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLMQNPELVKRMSESGVQAL
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHH
QNYRRLNIQAIERDIEMLIAKERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL
HHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCC
>Mature Secondary Structure
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHH
CFGCGAHGNAISFLMDYDKLEFIEAIEELAAMAGLEIPYEKRVNHSGKPQANYQTKRNLY
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHH
ELMQEIATFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIGFVPNAMDTVLRKFGVNREEQ
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
QKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HHHHHHHHHCCCCCCCHHHHHHCCEECEEECCCCCEEEECCEEECCCCCCCCCCCCEEEE
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLILR
ECCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
STEQVVCCYDGDRAGRDAAWRALENALPYLEDGRQLKFIFLPDGEEPDTYIRQYGKEKFE
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
EYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLIHQIPGEMLRLSLRNMLAQKL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHH
GIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLMQNPELVKRMSESGVQAL
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHH
QNYRRLNIQAIERDIEMLIAKERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL
HHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8294018; 7542800; 8316085