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The map label for this gene is thiE [H]

Identifier: 16272366

GI number: 16272366

Start: 439370

End: 440050

Strand: Direct

Name: thiE [H]

Synonym: HI0417

Alternate gene names: 16272366

Gene position: 439370-440050 (Clockwise)

Preceding gene: 16272365

Following gene: 16272367

Centisome position: 24.01

GC content: 42.88

Gene sequence:

>681_bases
ATGAAAAATATTCAAAAAATCTTACCGCTTTACTTTGTGGCAGGCACGCAAGATTGCCGTCATTTGGGCGAGAACTTGTC
GGAAAATTTATTATTCGTGCTTAAACAAGCGTTAGAAGGCGGAATTACCTGCTTTCAATTTCGGGATAAAGGCAAATTTT
CGTTAGAACATACGCCCTCAGCACAGAAGGCACTGGCGATTAATTGTCGTGATTTGTGCCGTGAATATGGCGTACCGTTT
ATTGTAGACGACAACGTTGATTTGGCGTTGGAAATCGAGGCGGATGGCATTCACGTTGGGCAAAGCGATATGCCTGTGCA
AGAGATTCGCGCTAAAACAGATAAACCGCTAATTATTGGTTGGTCGGTGAATCGTTTAGACGAGGCAAAAATTGGGGAAA
ATTTAGCAGAAATTGATTATTTTGGAATTGGTCCGATTTTCCCAACGCAATCAAAAGAAAATCCCAAACCAACACTAGGA
ATGGCATTTATTCAAACTTTGCGAAATGCGGGCATAACTAAACCGCTTGTGGCAATTGGTGGGGTGAAATTAGCTCACGT
TAAAACCTTACGAGAATTTGGGGCGGATGGTGTGGCAGTGATTACTGCAATTACGCACGCCGATAATGTGCAAGCCGCAA
CAAAAGCATTACGGGAGGCAAGTGATGAATACGCAAAATAG

Upstream 100 bases:

>100_bases
AAAGATTTTATTACCGCTGCAATTTCTCACCCATTGAATATTGGGCAAGGACATGGACCAACCAATCATTGGGCTTACAG
TCGCCTTTAAGGATTGAAAG

Downstream 100 bases:

>100_bases
TTTAAAGCAAGTAGCAACAGCGACGATGGTCGGCACTGCAATTGAGTATTTCGATAACTACATTTACGCAATGGCAGCGG
TATTGGTATTTAACCATCAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKNIQKILPLYFVAGTQDCRHLGENLSENLLFVLKQALEGGITCFQFRDKGKFSLEHTPSAQKALAINCRDLCREYGVPF
IVDDNVDLALEIEADGIHVGQSDMPVQEIRAKTDKPLIIGWSVNRLDEAKIGENLAEIDYFGIGPIFPTQSKENPKPTLG
MAFIQTLRNAGITKPLVAIGGVKLAHVKTLREFGADGVAVITAITHADNVQAATKALREASDEYAK

Sequences:

>Translated_226_residues
MKNIQKILPLYFVAGTQDCRHLGENLSENLLFVLKQALEGGITCFQFRDKGKFSLEHTPSAQKALAINCRDLCREYGVPF
IVDDNVDLALEIEADGIHVGQSDMPVQEIRAKTDKPLIIGWSVNRLDEAKIGENLAEIDYFGIGPIFPTQSKENPKPTLG
MAFIQTLRNAGITKPLVAIGGVKLAHVKTLREFGADGVAVITAITHADNVQAATKALREASDEYAK
>Mature_226_residues
MKNIQKILPLYFVAGTQDCRHLGENLSENLLFVLKQALEGGITCFQFRDKGKFSLEHTPSAQKALAINCRDLCREYGVPF
IVDDNVDLALEIEADGIHVGQSDMPVQEIRAKTDKPLIIGWSVNRLDEAKIGENLAEIDYFGIGPIFPTQSKENPKPTLG
MAFIQTLRNAGITKPLVAIGGVKLAHVKTLREFGADGVAVITAITHADNVQAATKALREASDEYAK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=187, Percent_Identity=29.9465240641711, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=203, Percent_Identity=33.0049261083744, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24731; Mature: 24731

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIQKILPLYFVAGTQDCRHLGENLSENLLFVLKQALEGGITCFQFRDKGKFSLEHTPS
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCC
AQKALAINCRDLCREYGVPFIVDDNVDLALEIEADGIHVGQSDMPVQEIRAKTDKPLIIG
HHHEEEECHHHHHHHCCCCEEEECCCCEEEEEECCCEEECCCCCCHHHHHHCCCCCEEEE
WSVNRLDEAKIGENLAEIDYFGIGPIFPTQSKENPKPTLGMAFIQTLRNAGITKPLVAIG
CCCCCCCHHHHCCCHHHCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHC
GVKLAHVKTLREFGADGVAVITAITHADNVQAATKALREASDEYAK
CCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNIQKILPLYFVAGTQDCRHLGENLSENLLFVLKQALEGGITCFQFRDKGKFSLEHTPS
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCC
AQKALAINCRDLCREYGVPFIVDDNVDLALEIEADGIHVGQSDMPVQEIRAKTDKPLIIG
HHHEEEECHHHHHHHCCCCEEEECCCCEEEEEECCCEEECCCCCCHHHHHHCCCCCEEEE
WSVNRLDEAKIGENLAEIDYFGIGPIFPTQSKENPKPTLGMAFIQTLRNAGITKPLVAIG
CCCCCCCHHHHCCCHHHCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHC
GVKLAHVKTLREFGADGVAVITAITHADNVQAATKALREASDEYAK
CCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA