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The map label for this gene is thiD

Identifier: 16272365

GI number: 16272365

Start: 438550

End: 439359

Strand: Direct

Name: thiD

Synonym: HI0416

Alternate gene names: 16272365

Gene position: 438550-439359 (Clockwise)

Preceding gene: 16272364

Following gene: 16272366

Centisome position: 23.96

GC content: 46.91

Gene sequence:

>810_bases
ATGAGTAATGTGAAACAAGTTTTAACCATCGCAGGGTCAGACAGTGGCGGTGGTGCAGGCATTCAAGCGGATCTTAAAAC
CTTCCAAATGCGCGGCGTGTTTGGTACATCAGCAATCACGGCAGTTACGGCACAAAACACACTTGGCGTATTTGATATTC
ACCCTATTCCACTGAAAACAATTCAAGCGCAACTGGAAGCGGTCAAAAATGATTTCCAGATTGCAAGTTGCAAAATTGGT
ATGTTGGGCAATGCAGAAATTATTGAATGCGTCGCAGATTTTCTTGCTGATAAGCCTTTCGGCACGCTGGTGCTAGATCC
TGTGATGATCGCCAAAGGTGGCGCACCGTTATTGCAACGGCAAGCGGTGAGTGCGTTAAGCCAAAAATTGCTGCCGTTGG
CGGACGTGATTACACCGAACATTCCCGAAGCGGAAGCATTAACGGGGATTGCGATTGTGGACGACATCAGTATTCAACAA
GCCGCAAAAGCGTTGCAAAAACAAGGAGCGAAGAATGTGATTATTAAGGGCGGACATTCGCTCAATTCGCAAAGTGAGCT
GTGCCAAGATTGGGTTTTACTTGCAGATGGTCGTCATTTTACTTTGCAAAGCCCCCGTTTCAACACGCCGCATACACATG
GTACGGGCTGTACCTTTTCTGCCTGTTTAACCGCAGAGTTGGCGAAAGGCGAACCGTTGCAAAGTGCGGTAAAAACAGCG
AAAGATTTTATTACCGCTGCAATTTCTCACCCATTGAATATTGGGCAAGGACATGGACCAACCAATCATTGGGCTTACAG
TCGCCTTTAA

Upstream 100 bases:

>100_bases
GCGCAAGGCTTAACCACACAAGTGGGACAGTTCCAAATCCGTTTGTTGGACGAGCTTGCTGCCCTTTCCCCTGAAACCAT
TGGACAAAGAGGGCGCATCA

Downstream 100 bases:

>100_bases
GGATTGAAAGATGAAAAATATTCAAAAAATCTTACCGCTTTACTTTGTGGCAGGCACGCAAGATTGCCGTCATTTGGGCG
AGAACTTGTCGGAAAATTTA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSNVKQVLTIAGSDSGGGAGIQADLKTFQMRGVFGTSAITAVTAQNTLGVFDIHPIPLKTIQAQLEAVKNDFQIASCKIG
MLGNAEIIECVADFLADKPFGTLVLDPVMIAKGGAPLLQRQAVSALSQKLLPLADVITPNIPEAEALTGIAIVDDISIQQ
AAKALQKQGAKNVIIKGGHSLNSQSELCQDWVLLADGRHFTLQSPRFNTPHTHGTGCTFSACLTAELAKGEPLQSAVKTA
KDFITAAISHPLNIGQGHGPTNHWAYSRL

Sequences:

>Translated_269_residues
MSNVKQVLTIAGSDSGGGAGIQADLKTFQMRGVFGTSAITAVTAQNTLGVFDIHPIPLKTIQAQLEAVKNDFQIASCKIG
MLGNAEIIECVADFLADKPFGTLVLDPVMIAKGGAPLLQRQAVSALSQKLLPLADVITPNIPEAEALTGIAIVDDISIQQ
AAKALQKQGAKNVIIKGGHSLNSQSELCQDWVLLADGRHFTLQSPRFNTPHTHGTGCTFSACLTAELAKGEPLQSAVKTA
KDFITAAISHPLNIGQGHGPTNHWAYSRL
>Mature_268_residues
SNVKQVLTIAGSDSGGGAGIQADLKTFQMRGVFGTSAITAVTAQNTLGVFDIHPIPLKTIQAQLEAVKNDFQIASCKIGM
LGNAEIIECVADFLADKPFGTLVLDPVMIAKGGAPLLQRQAVSALSQKLLPLADVITPNIPEAEALTGIAIVDDISIQQA
AKALQKQGAKNVIIKGGHSLNSQSELCQDWVLLADGRHFTLQSPRFNTPHTHGTGCTFSACLTAELAKGEPLQSAVKTAK
DFITAAISHPLNIGQGHGPTNHWAYSRL

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family

Homologues:

Organism=Escherichia coli, GI1788420, Length=261, Percent_Identity=43.2950191570881, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1788758, Length=247, Percent_Identity=30.3643724696356, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325378, Length=272, Percent_Identity=30.8823529411765, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324517, Length=273, Percent_Identity=34.7985347985348, Blast_Score=113, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324997, Length=273, Percent_Identity=33.6996336996337, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THID_HAEIN (P44697)

Other databases:

- EMBL:   L42023
- PIR:   I64151
- RefSeq:   NP_438578.1
- ProteinModelPortal:   P44697
- SMR:   P44697
- GeneID:   949517
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0416
- NMPDR:   fig|71421.1.peg.391
- TIGR:   HI_0416
- HOGENOM:   HBG476673
- OMA:   LNIGHGH
- ProtClustDB:   PRK06427
- BioCyc:   HINF71421:HI_0416-MONOMER
- BRENDA:   2.7.4.7
- InterPro:   IPR013749
- InterPro:   IPR004399
- TIGRFAMs:   TIGR00097

Pfam domain/function: PF08543 Phos_pyr_kin

EC number: =2.7.1.49; =2.7.4.7

Molecular weight: Translated: 28295; Mature: 28164

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: BINDING 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVKQVLTIAGSDSGGGAGIQADLKTFQMRGVFGTSAITAVTAQNTLGVFDIHPIPLKT
CCCHHEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHEEEEECCCCCEEECCCCCHHH
IQAQLEAVKNDFQIASCKIGMLGNAEIIECVADFLADKPFGTLVLDPVMIAKGGAPLLQR
HHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEECHHHEECCCCHHHHH
QAVSALSQKLLPLADVITPNIPEAEALTGIAIVDDISIQQAAKALQKQGAKNVIIKGGHS
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCC
LNSQSELCQDWVLLADGRHFTLQSPRFNTPHTHGTGCTFSACLTAELAKGEPLQSAVKTA
CCCHHHHHHHHEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
KDFITAAISHPLNIGQGHGPTNHWAYSRL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNVKQVLTIAGSDSGGGAGIQADLKTFQMRGVFGTSAITAVTAQNTLGVFDIHPIPLKT
CCHHEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHEEEEECCCCCEEECCCCCHHH
IQAQLEAVKNDFQIASCKIGMLGNAEIIECVADFLADKPFGTLVLDPVMIAKGGAPLLQR
HHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEECHHHEECCCCHHHHH
QAVSALSQKLLPLADVITPNIPEAEALTGIAIVDDISIQQAAKALQKQGAKNVIIKGGHS
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCC
LNSQSELCQDWVLLADGRHFTLQSPRFNTPHTHGTGCTFSACLTAELAKGEPLQSAVKTA
CCCHHHHHHHHEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
KDFITAAISHPLNIGQGHGPTNHWAYSRL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800