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The map label for this gene is thiM [H]

Identifier: 16272364

GI number: 16272364

Start: 437760

End: 438557

Strand: Direct

Name: thiM [H]

Synonym: HI0415

Alternate gene names: 16272364

Gene position: 437760-438557 (Clockwise)

Preceding gene: 16272363

Following gene: 16272365

Centisome position: 23.92

GC content: 48.25

Gene sequence:

>798_bases
ATGCTTATGCAATCAATTTATCTCTCCAAAATCCGTGAGCAGAATCCGCTCATTCATAACATTACCAATATTGTAGCAGC
GAATTTTAGTGCCAATGGCTTGTTGGCATTAGGAGCCTCGCCTTTGATGTCGGCGAATGTTGAAGAAATGCAGGAAGTAC
CGAAAATCAGTCAAGCATTGGTCATTAATATTGGTACGCTGATTGGTAAAGATCGGGAGGCGATGTTGCAGGCAGGAAAA
ACAGCAAATGAGGTTGGAATTCCTGTAGTGTTAGATCCTGTTGGTGTGGGGGCGACCAGTTATCGCCGTGAGACCATTCG
TGAGCTTTTAGCCGAGGTAAAATTCGCACTTATTCGTGGTAATGCAGGTGAACTGGCTGCCATTGCAGGCGAGACGTGGC
AGGCAAAAGGTGTAGATGCAGGGCAAGGCGAAGTGGATTTAAAAGCCGTTGCCGAGAAAGTGGCTCAACGCTATGGCTGT
ACGGTGTTGATTAGCGGGGCGGTGGATATTGTCAGCGATGGCACACAAACTGCGACCGTTCACAACGGCACGTCATTGTT
CCCGAAAGTTACGGCTTCGGGCTGTTTATTGAGTGCTGTGTGTGCGGCATTTTTAGCAGTAAGTGAAGGGAATTATTTTT
CTGCGACATTAGAAGCCTGTGTAGCATACACCATTGCAGGCGAGTGTGCGGCGCAAGGCTTAACCACACAAGTGGGACAG
TTCCAAATCCGTTTGTTGGACGAGCTTGCTGCCCTTTCCCCTGAAACCATTGGACAAAGAGGGCGCATCAATGAGTAA

Upstream 100 bases:

>100_bases
TATCCTGTTTATTTTCCCAATTTTCTACATTTTATTACACCTGCTAATTGCTCACAGTGAATAGCGGGCATTTTTGTTTA
TTTTATATTCAACACAAGGA

Downstream 100 bases:

>100_bases
TGTGAAACAAGTTTTAACCATCGCAGGGTCAGACAGTGGCGGTGGTGCAGGCATTCAAGCGGATCTTAAAACCTTCCAAA
TGCGCGGCGTGTTTGGTACA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLMQSIYLSKIREQNPLIHNITNIVAANFSANGLLALGASPLMSANVEEMQEVPKISQALVINIGTLIGKDREAMLQAGK
TANEVGIPVVLDPVGVGATSYRRETIRELLAEVKFALIRGNAGELAAIAGETWQAKGVDAGQGEVDLKAVAEKVAQRYGC
TVLISGAVDIVSDGTQTATVHNGTSLFPKVTASGCLLSAVCAAFLAVSEGNYFSATLEACVAYTIAGECAAQGLTTQVGQ
FQIRLLDELAALSPETIGQRGRINE

Sequences:

>Translated_265_residues
MLMQSIYLSKIREQNPLIHNITNIVAANFSANGLLALGASPLMSANVEEMQEVPKISQALVINIGTLIGKDREAMLQAGK
TANEVGIPVVLDPVGVGATSYRRETIRELLAEVKFALIRGNAGELAAIAGETWQAKGVDAGQGEVDLKAVAEKVAQRYGC
TVLISGAVDIVSDGTQTATVHNGTSLFPKVTASGCLLSAVCAAFLAVSEGNYFSATLEACVAYTIAGECAAQGLTTQVGQ
FQIRLLDELAALSPETIGQRGRINE
>Mature_265_residues
MLMQSIYLSKIREQNPLIHNITNIVAANFSANGLLALGASPLMSANVEEMQEVPKISQALVINIGTLIGKDREAMLQAGK
TANEVGIPVVLDPVGVGATSYRRETIRELLAEVKFALIRGNAGELAAIAGETWQAKGVDAGQGEVDLKAVAEKVAQRYGC
TVLISGAVDIVSDGTQTATVHNGTSLFPKVTASGCLLSAVCAAFLAVSEGNYFSATLEACVAYTIAGECAAQGLTTQVGQ
FQIRLLDELAALSPETIGQRGRINE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=248, Percent_Identity=39.5161290322581, Blast_Score=148, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6325042, Length=284, Percent_Identity=32.0422535211268, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 27733; Mature: 27733

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMQSIYLSKIREQNPLIHNITNIVAANFSANGLLALGASPLMSANVEEMQEVPKISQAL
CCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCHHCCCHHHHHHHHHHHHHH
VINIGTLIGKDREAMLQAGKTANEVGIPVVLDPVGVGATSYRRETIRELLAEVKFALIRG
HEEHHHHHCCCHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEC
NAGELAAIAGETWQAKGVDAGQGEVDLKAVAEKVAQRYGCTVLISGAVDIVSDGTQTATV
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEE
HNGTSLFPKVTASGCLLSAVCAAFLAVSEGNYFSATLEACVAYTIAGECAAQGLTTQVGQ
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FQIRLLDELAALSPETIGQRGRINE
HHHHHHHHHHHCCHHHCCCCCCCCC
>Mature Secondary Structure
MLMQSIYLSKIREQNPLIHNITNIVAANFSANGLLALGASPLMSANVEEMQEVPKISQAL
CCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCHHCCCHHHHHHHHHHHHHH
VINIGTLIGKDREAMLQAGKTANEVGIPVVLDPVGVGATSYRRETIRELLAEVKFALIRG
HEEHHHHHCCCHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEC
NAGELAAIAGETWQAKGVDAGQGEVDLKAVAEKVAQRYGCTVLISGAVDIVSDGTQTATV
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEE
HNGTSLFPKVTASGCLLSAVCAAFLAVSEGNYFSATLEACVAYTIAGECAAQGLTTQVGQ
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FQIRLLDELAALSPETIGQRGRINE
HHHHHHHHHHHCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA