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The map label for this gene is 16272306

Identifier: 16272306

GI number: 16272306

Start: 382121

End: 383065

Strand: Direct

Name: 16272306

Synonym: HI0357

Alternate gene names: NA

Gene position: 382121-383065 (Clockwise)

Preceding gene: 16272305

Following gene: 16272307

Centisome position: 20.88

GC content: 39.58

Gene sequence:

>945_bases
ATGAAGACAATCATCCGTTATTTCAGCTTTGTAATGGGGCTAATGCTCACTCTTCCTAGCTTTGCAAAAGAGAAAATCAG
CATAATGCTAGATTGGTATGTAAACCCCGATCATGCAGCCATCATTGTGGCTCAACAAAAAGGCTTTTTTGAAAAAAATA
ACTTGGAGGTTGAAATTATCGAACCTGCTGATCCTGCATTACCGCCGAAATTAGCTGCGGCAGAAAAAGTTGATTTAGCC
GTGAGTTATCAGCCTCAGCTTTATCAACAAGTGGCAGAAGGCTTGCCTTTAGTGCGAGTGGGTTCACTGATTTCAAATCC
ATTAAATAGCGTAGTGGTACTAAAAAAGAGCAATTTGAAAAGCCTTGCTGATTTAAAAGGTAAAAAAGTGGGGTATTCTG
TCAGCGGTTTTGAAGATGGATTACTTGATACAATGCTTCATTCTATTGGTTTAAGCAATAAAGATGTGGAATTAGTGAAT
GTAAATTGGTCGCTTTCGCCCTCTCTTTTAACTGGACAAGTGGATGCTGTGATTGGTGCATTTCGTAATTTTGAACTCAA
TCAGCTTGCTTTAGAAAAGCAAGAAGGTATCGCATTCTTCCCTGAACAATATGGCGTGCCTGCTTATGATGAGTTGATTT
TAGTTGCTAATAAAAACAGCGTAACAGATAAAAAAACATCGGCATTTTTGACCGCACTTGAACAAGCCACAAGCTATCTA
CAAGCTCACCCCAATGAAGCGTGGCAAGCCTTTGTCAGCTATAAACCAAACGAACTCAACACGCCATTAAACCAACTGGC
GTGGAAAGACACGCTCCCCTTTTTAGCCAATAAACCTCGCCAATTAGACGCTAAACGTTATCAACAAATGGCAGAATTTA
TGCAACAAAAAGGCTTAATTCCAAAAGCCTTAGCCTTGAAAGAATATGCGGTAGAAATTGAATAA

Upstream 100 bases:

>100_bases
ACTGATTTTAGTGTCAATTTCACTCTGTTTATATTTCAGCATTGATTGGCTATTACACCGTTTTATTTGGTCTGTTTAAC
TTTTCATTAAAGGAGAAAAA

Downstream 100 bases:

>100_bases
GGAAAAAAGATGATTGAACAACTCATTCAACAAGCTCAGCCTTATTGGCAACAATATATTGAACACGAATTTGTGCAACA
GCTCGCAAAAGGTACGCTAC

Product: thiamine biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKTIIRYFSFVMGLMLTLPSFAKEKISIMLDWYVNPDHAAIIVAQQKGFFEKNNLEVEIIEPADPALPPKLAAAEKVDLA
VSYQPQLYQQVAEGLPLVRVGSLISNPLNSVVVLKKSNLKSLADLKGKKVGYSVSGFEDGLLDTMLHSIGLSNKDVELVN
VNWSLSPSLLTGQVDAVIGAFRNFELNQLALEKQEGIAFFPEQYGVPAYDELILVANKNSVTDKKTSAFLTALEQATSYL
QAHPNEAWQAFVSYKPNELNTPLNQLAWKDTLPFLANKPRQLDAKRYQQMAEFMQQKGLIPKALALKEYAVEIE

Sequences:

>Translated_314_residues
MKTIIRYFSFVMGLMLTLPSFAKEKISIMLDWYVNPDHAAIIVAQQKGFFEKNNLEVEIIEPADPALPPKLAAAEKVDLA
VSYQPQLYQQVAEGLPLVRVGSLISNPLNSVVVLKKSNLKSLADLKGKKVGYSVSGFEDGLLDTMLHSIGLSNKDVELVN
VNWSLSPSLLTGQVDAVIGAFRNFELNQLALEKQEGIAFFPEQYGVPAYDELILVANKNSVTDKKTSAFLTALEQATSYL
QAHPNEAWQAFVSYKPNELNTPLNQLAWKDTLPFLANKPRQLDAKRYQQMAEFMQQKGLIPKALALKEYAVEIE
>Mature_314_residues
MKTIIRYFSFVMGLMLTLPSFAKEKISIMLDWYVNPDHAAIIVAQQKGFFEKNNLEVEIIEPADPALPPKLAAAEKVDLA
VSYQPQLYQQVAEGLPLVRVGSLISNPLNSVVVLKKSNLKSLADLKGKKVGYSVSGFEDGLLDTMLHSIGLSNKDVELVN
VNWSLSPSLLTGQVDAVIGAFRNFELNQLALEKQEGIAFFPEQYGVPAYDELILVANKNSVTDKKTSAFLTALEQATSYL
QAHPNEAWQAFVSYKPNELNTPLNQLAWKDTLPFLANKPRQLDAKRYQQMAEFMQQKGLIPKALALKEYAVEIE

Specific function: Probably involved in thiamine biosynthesis

COG id: COG0715

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NMT1 family

Homologues:

Organism=Saccharomyces cerevisiae, GI6319957, Length=269, Percent_Identity=27.5092936802974, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321050, Length=269, Percent_Identity=27.5092936802974, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323997, Length=269, Percent_Identity=27.5092936802974, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6322616, Length=269, Percent_Identity=27.1375464684015, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y357_HAEIN (P44658)

Other databases:

- EMBL:   L42023
- PIR:   C64063
- RefSeq:   NP_438519.1
- ProteinModelPortal:   P44658
- GeneID:   949462
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0357
- NMPDR:   fig|71421.1.peg.332
- TIGR:   HI_0357
- HOGENOM:   HBG678689
- OMA:   PNAVHSF
- ProtClustDB:   CLSK2518357
- BioCyc:   HINF71421:HI_0357-MONOMER
- InterPro:   IPR015168

Pfam domain/function: PF09084 NMT1

EC number: NA

Molecular weight: Translated: 34962; Mature: 34962

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIIRYFSFVMGLMLTLPSFAKEKISIMLDWYVNPDHAAIIVAQQKGFFEKNNLEVEII
CHHHHHHHHHHHHHHHHCCHHHHCEEEEEEEEEECCCCEEEEEEECCCCEECCCCEEEEE
EPADPALPPKLAAAEKVDLAVSYQPQLYQQVAEGLPLVRVGSLISNPLNSVVVLKKSNLK
CCCCCCCCCCHHCCCCEEEEEECCHHHHHHHHCCCCEEEHHHHHHCCCCCEEEEECCCHH
SLADLKGKKVGYSVSGFEDGLLDTMLHSIGLSNKDVELVNVNWSLSPSLLTGQVDAVIGA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
FRNFELNQLALEKQEGIAFFPEQYGVPAYDELILVANKNSVTDKKTSAFLTALEQATSYL
HHCCCHHHHHHHHCCCCEECHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
QAHPNEAWQAFVSYKPNELNTPLNQLAWKDTLPFLANKPRQLDAKRYQQMAEFMQQKGLI
HCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCC
PKALALKEYAVEIE
CHHHHHHHHHEECC
>Mature Secondary Structure
MKTIIRYFSFVMGLMLTLPSFAKEKISIMLDWYVNPDHAAIIVAQQKGFFEKNNLEVEII
CHHHHHHHHHHHHHHHHCCHHHHCEEEEEEEEEECCCCEEEEEEECCCCEECCCCEEEEE
EPADPALPPKLAAAEKVDLAVSYQPQLYQQVAEGLPLVRVGSLISNPLNSVVVLKKSNLK
CCCCCCCCCCHHCCCCEEEEEECCHHHHHHHHCCCCEEEHHHHHHCCCCCEEEEECCCHH
SLADLKGKKVGYSVSGFEDGLLDTMLHSIGLSNKDVELVNVNWSLSPSLLTGQVDAVIGA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
FRNFELNQLALEKQEGIAFFPEQYGVPAYDELILVANKNSVTDKKTSAFLTALEQATSYL
HHCCCHHHHHHHHCCCCEECHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
QAHPNEAWQAFVSYKPNELNTPLNQLAWKDTLPFLANKPRQLDAKRYQQMAEFMQQKGLI
HCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCC
PKALALKEYAVEIE
CHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023