Click here to switch to the map view.

The map label for this gene is napC

Identifier: 16272299

GI number: 16272299

Start: 375133

End: 375735

Strand: Direct

Name: napC

Synonym: HI0348

Alternate gene names: 16272299

Gene position: 375133-375735 (Clockwise)

Preceding gene: 16272298

Following gene: 16272304

Centisome position: 20.5

GC content: 39.14

Gene sequence:

>603_bases
ATGTCTGAGAAAAAACCAAATATCTTAAAGCGTTTCTGGCAATGGTTTAGAAAACCAAGCCGTATGGCGATAGGTACAAT
TATTATCTTATCGGCTATTGGCGGTATTCTTTCTTGGGTTGGTTTTAACTATGGTTTAGAAAAAACCAACACTGAACAGT
TCTGTGCAAGCTGCCATATGCAAGATGCTTATCCAGAATATTTACATAGCGTGCATTATCAAACTCGTACTGGTGTGGGG
GCAAGTTGTCCAGATTGTCACGTACCACACGAATTTGGTGCAAAAATGAAACGTAAAATTATTGCAGCTAAAGAAGTATA
TGCGCACTATACTGGAAAAGTGGATACCTTAGAAAAATTCAATGCACATCGTTTAGAAATGGCTCAAAATGAATGGGCGC
GTATGAAAGCTAACGATTCTAAAGAATGTCGTAATTGCCATAATGTAGATCGTATGACCTTCAATGATCAACGTTCTGTG
GCGGCACGTATGCACCAAAAAATGAAAACTGAAGGTAAAACTTGTATCGACTGTCACAAAGGTATTGCACACCAATTACC
TGATATGAGTGGCGTTGAGTCAGGCTTCAAAGACGAGAAGTGA

Upstream 100 bases:

>100_bases
TCTGTTTACAATGTCACGTTTCTCAAGCAAACGTAGATCCGATTGTTCCAAATGATTTCAAACCGATGAAAGGTTACGGA
AACTAATTTAAGAGGTCTAT

Downstream 100 bases:

>100_bases
TTTTCTAAAAATAGGCTGAAAAAATATCGCACTTTTTTCAGTCTATTATAAATTTGGGTAGTTTATAAAAAAATCTGCAC
TTTAATTCGCTGATTTATTC

Product: cytochrome C-type protein

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSEKKPNILKRFWQWFRKPSRMAIGTIIILSAIGGILSWVGFNYGLEKTNTEQFCASCHMQDAYPEYLHSVHYQTRTGVG
ASCPDCHVPHEFGAKMKRKIIAAKEVYAHYTGKVDTLEKFNAHRLEMAQNEWARMKANDSKECRNCHNVDRMTFNDQRSV
AARMHQKMKTEGKTCIDCHKGIAHQLPDMSGVESGFKDEK

Sequences:

>Translated_200_residues
MSEKKPNILKRFWQWFRKPSRMAIGTIIILSAIGGILSWVGFNYGLEKTNTEQFCASCHMQDAYPEYLHSVHYQTRTGVG
ASCPDCHVPHEFGAKMKRKIIAAKEVYAHYTGKVDTLEKFNAHRLEMAQNEWARMKANDSKECRNCHNVDRMTFNDQRSV
AARMHQKMKTEGKTCIDCHKGIAHQLPDMSGVESGFKDEK
>Mature_199_residues
SEKKPNILKRFWQWFRKPSRMAIGTIIILSAIGGILSWVGFNYGLEKTNTEQFCASCHMQDAYPEYLHSVHYQTRTGVGA
SCPDCHVPHEFGAKMKRKIIAAKEVYAHYTGKVDTLEKFNAHRLEMAQNEWARMKANDSKECRNCHNVDRMTFNDQRSVA
ARMHQKMKTEGKTCIDCHKGIAHQLPDMSGVESGFKDEK

Specific function: Mediates electron flow from quinones to the NapAB complex

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788530, Length=197, Percent_Identity=58.8832487309645, Blast_Score=260, Evalue=4e-71,
Organism=Escherichia coli, GI1787230, Length=194, Percent_Identity=43.8144329896907, Blast_Score=171, Evalue=3e-44,
Organism=Escherichia coli, GI1788181, Length=146, Percent_Identity=43.1506849315069, Blast_Score=133, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPC_HAEIN (P44655)

Other databases:

- EMBL:   L42023
- PIR:   H64062
- RefSeq:   NP_438512.1
- ProteinModelPortal:   P44655
- SMR:   P44655
- GeneID:   949803
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0348
- NMPDR:   fig|71421.1.peg.325
- TIGR:   HI_0348
- HOGENOM:   HBG297153
- OMA:   CTGCHEM
- ProtClustDB:   CLSK789812
- BioCyc:   HINF71421:HI_0348-MONOMER
- GO:   GO:0006810
- InterPro:   IPR011031
- InterPro:   IPR005126
- InterPro:   IPR011885
- TIGRFAMs:   TIGR02161

Pfam domain/function: PF03264 Cytochrom_NNT

EC number: NA

Molecular weight: Translated: 22958; Mature: 22826

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 55-55 BINDING 58-58 BINDING 83-83 BINDING 86-86 BINDING 143-143 BINDING 146-146 BINDING 175-175 BINDING 178-178

Signals:

None

Transmembrane regions:

HASH(0xafed284)-;

Cys/Met content:

4.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKPNILKRFWQWFRKPSRMAIGTIIILSAIGGILSWVGFNYGLEKTNTEQFCASCHM
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
QDAYPEYLHSVHYQTRTGVGASCPDCHVPHEFGAKMKRKIIAAKEVYAHYTGKVDTLEKF
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NAHRLEMAQNEWARMKANDSKECRNCHNVDRMTFNDQRSVAARMHQKMKTEGKTCIDCHK
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
GIAHQLPDMSGVESGFKDEK
HHHHHCCCCCHHHCCCCCCH
>Mature Secondary Structure 
SEKKPNILKRFWQWFRKPSRMAIGTIIILSAIGGILSWVGFNYGLEKTNTEQFCASCHM
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
QDAYPEYLHSVHYQTRTGVGASCPDCHVPHEFGAKMKRKIIAAKEVYAHYTGKVDTLEKF
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NAHRLEMAQNEWARMKANDSKECRNCHNVDRMTFNDQRSVAARMHQKMKTEGKTCIDCHK
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
GIAHQLPDMSGVESGFKDEK
HHHHHCCCCCHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800