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The map label for this gene is napH

Identifier: 16272297

GI number: 16272297

Start: 373806

End: 374669

Strand: Direct

Name: napH

Synonym: HI0346

Alternate gene names: 16272297

Gene position: 373806-374669 (Clockwise)

Preceding gene: 16272296

Following gene: 16272298

Centisome position: 20.43

GC content: 41.9

Gene sequence:

>864_bases
ATGGCAAATGCACCAAAATTTGCAGGGAAAGAATCGCGCGAAAAATGGGGCTGGTGGTATGCAAACCGCTTTTTGTTTTG
GCGTCGATTAAGCCAACTTAGCATTCTTGCGATGTTTTTGAGTGGCCCTTATTTTGGCGTGTGGATTTTAAAAGGTAATT
ATAGTGGTAGTTTATTACTCGATACTATTCCACTAAGTGATCCTCTAATTACCGCTGAAAGCTTGGCTGCAAGACATTTA
CCAGATGCTCTCACTTTAATTGGTGCTGCAATTATTGTGTTATTTTACGCAGTACTTGGTAGTAAAGTGTTTTGTGGTTG
GGTATGCCCACTAAATGTTGTGACTGACTGTGCAGCGTGGCTTCGCCGTAAGTTAGGCATTCGCCAAACAGCAAAAATTT
CGCGCGGTTTACGTTATGGTATTCTTGTGCTTATTTTACTAGGCAGTAGTGTCAGCGGAATGTTGCTTTGGGAGTGGGTT
AATCCAGTGGCTGCTCTTGGTCGCGCCTTTGTATTTGGTTTTGGTGCAACAGGCTGGTTATTACTTGTTATTTTTCTTTT
TGATTTATTGATTGCTGAACACGGCTGGTGTGGACATCTTTGCCCAATTGGTGCTGCTTATGGTGTGATTGGTGCAAAAA
GTTTAATCCGTATTAAAGTGATTGATCGCGCTAAATGCGATAATTGTATGGATTGTTACAATGTTTGTCCCGAAGCCCAA
GTGTTACGTTCGCCTTTACACGGAAAAAAAGACGAAAGCCTACTAGTGCTTTCCAAAGATTGCATCAGCTGCGGACGTTG
TATTGACGTTTGCGCTGAAAAAGTTTTTAAATTTTCGACCAGATTCGATCATTCAGGGGAGTGA

Upstream 100 bases:

>100_bases
AAGATATGATTTCATTGCCAACTCGAACACCAGAAGGAACAACTGTAATTCCTGAACCAGCAGAACCCGTGCTTGCGCCT
ATTTTAGGGAGTGGCAAATA

Downstream 100 bases:

>100_bases
TTAATATGACTAAACAGGTATCTAAAATTTTAGCGGGATTATTTACCGCACTTTTTGCTGGCTCACTGATGGCATCTGAT
GCACCAGCAGTGGGAAAAGA

Product: quinol dehydrogenase membrane component

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MANAPKFAGKESREKWGWWYANRFLFWRRLSQLSILAMFLSGPYFGVWILKGNYSGSLLLDTIPLSDPLITAESLAARHL
PDALTLIGAAIIVLFYAVLGSKVFCGWVCPLNVVTDCAAWLRRKLGIRQTAKISRGLRYGILVLILLGSSVSGMLLWEWV
NPVAALGRAFVFGFGATGWLLLVIFLFDLLIAEHGWCGHLCPIGAAYGVIGAKSLIRIKVIDRAKCDNCMDCYNVCPEAQ
VLRSPLHGKKDESLLVLSKDCISCGRCIDVCAEKVFKFSTRFDHSGE

Sequences:

>Translated_287_residues
MANAPKFAGKESREKWGWWYANRFLFWRRLSQLSILAMFLSGPYFGVWILKGNYSGSLLLDTIPLSDPLITAESLAARHL
PDALTLIGAAIIVLFYAVLGSKVFCGWVCPLNVVTDCAAWLRRKLGIRQTAKISRGLRYGILVLILLGSSVSGMLLWEWV
NPVAALGRAFVFGFGATGWLLLVIFLFDLLIAEHGWCGHLCPIGAAYGVIGAKSLIRIKVIDRAKCDNCMDCYNVCPEAQ
VLRSPLHGKKDESLLVLSKDCISCGRCIDVCAEKVFKFSTRFDHSGE
>Mature_286_residues
ANAPKFAGKESREKWGWWYANRFLFWRRLSQLSILAMFLSGPYFGVWILKGNYSGSLLLDTIPLSDPLITAESLAARHLP
DALTLIGAAIIVLFYAVLGSKVFCGWVCPLNVVTDCAAWLRRKLGIRQTAKISRGLRYGILVLILLGSSVSGMLLWEWVN
PVAALGRAFVFGFGATGWLLLVIFLFDLLIAEHGWCGHLCPIGAAYGVIGAKSLIRIKVIDRAKCDNCMDCYNVCPEAQV
LRSPLHGKKDESLLVLSKDCISCGRCIDVCAEKVFKFSTRFDHSGE

Specific function: Involved in electron transfer

COG id: COG0348

COG function: function code C; Polyferredoxin

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1788532, Length=287, Percent_Identity=54.7038327526132, Blast_Score=332, Evalue=1e-92,
Organism=Escherichia coli, GI1787226, Length=269, Percent_Identity=24.907063197026, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPH_HAEIN (P44653)

Other databases:

- EMBL:   L42023
- PIR:   B64149
- RefSeq:   NP_438510.1
- ProteinModelPortal:   P44653
- SMR:   P44653
- GeneID:   949454
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0346
- NMPDR:   fig|71421.1.peg.323
- TIGR:   HI_0346
- HOGENOM:   HBG296648
- OMA:   WEWVNPV
- ProtClustDB:   PRK09477
- BioCyc:   HINF71421:HI_0346-MONOMER
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR011886
- TIGRFAMs:   TIGR02163

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 31753; Mature: 31622

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc472a44)-; HASH(0xb937504)-; HASH(0xc340a08)-; HASH(0xc1e949c)-;

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANAPKFAGKESREKWGWWYANRFLFWRRLSQLSILAMFLSGPYFGVWILKGNYSGSLLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEE
DTIPLSDPLITAESLAARHLPDALTLIGAAIIVLFYAVLGSKVFCGWVCPLNVVTDCAAW
EECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHH
LRRKLGIRQTAKISRGLRYGILVLILLGSSVSGMLLWEWVNPVAALGRAFVFGFGATGWL
HHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
LLVIFLFDLLIAEHGWCGHLCPIGAAYGVIGAKSLIRIKVIDRAKCDNCMDCYNVCPEAQ
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHCCCCHH
VLRSPLHGKKDESLLVLSKDCISCGRCIDVCAEKVFKFSTRFDHSGE
HHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure 
ANAPKFAGKESREKWGWWYANRFLFWRRLSQLSILAMFLSGPYFGVWILKGNYSGSLLL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEE
DTIPLSDPLITAESLAARHLPDALTLIGAAIIVLFYAVLGSKVFCGWVCPLNVVTDCAAW
EECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHH
LRRKLGIRQTAKISRGLRYGILVLILLGSSVSGMLLWEWVNPVAALGRAFVFGFGATGWL
HHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
LLVIFLFDLLIAEHGWCGHLCPIGAAYGVIGAKSLIRIKVIDRAKCDNCMDCYNVCPEAQ
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHCCCCHH
VLRSPLHGKKDESLLVLSKDCISCGRCIDVCAEKVFKFSTRFDHSGE
HHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800