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The map label for this gene is rnt [H]

Identifier: 16272277

GI number: 16272277

Start: 352323

End: 353012

Strand: Direct

Name: rnt [H]

Synonym: HI0324

Alternate gene names: 16272277

Gene position: 352323-353012 (Clockwise)

Preceding gene: 16272276

Following gene: 16272278

Centisome position: 19.25

GC content: 41.3

Gene sequence:

>690_bases
ATGTCCGATTCTCAAGAAATCCCTTATCACAACCAATTAAAAAATCGCTTTCGTGGCTATTTCCCCGTCATTATTGATGT
TGAAACTGCAGGTTTTGATGCAAAAAAAGATGCGTTACTCGAACTAGCCGCCATCACATTAAAAATGGATGAAAACGGTT
ATTTGCATCCTGATCAGAAATGCCATTTTCATATCAAGCCTTTTGAAGGCGCAAATATTAATCCAGAATCCTTGAAATTT
AATGGTATTGATATTCACAATCCACTACGTGGCGCGGTGTCTGAACTTGATGCAATCACGGGATTATTTCAAATGGTGCG
TCGAGGACAAAAAGATGCGGATTGCCAACGTTCTATCATTGTGGCGCACAATGCCGCTTTTGACCAAAGTTTTGTGATGG
CTGCCGCTGAACGCACTGGCGTAAAGCGTAATCCCTTCCACCCTTTTGGCATGTTTGATACCGCAAGTTTAGCGGGCTTA
ATGTTTGGTCAGACTGTTCTCGTTAAAGCCTGCCAAGCGGCAAAAATTCCTTTTGATGGTAAACAAGCCCATTCAGCGTT
ATATGATACCGAACGCACGGCGAAATTATTCTGCTATATGGTCAATCATTTAAAAGATTTAGGTGGTTTCCCACATATTG
CGTCTGAACTAGAACAGGAAAAAACAACTGAAAAAGAGACCGCACTTTAA

Upstream 100 bases:

>100_bases
AGTACTAAATCTGGTTTAGGCAACTAAAGTGCGGTCAATTTTTATTGTATTTTTAATAACGTAGGGATTCCTACGTTATT
TTATTTTTAAGAGATAGATT

Downstream 100 bases:

>100_bases
GAAAAATAAAAAACTGGTTGCAATCGTTTTATTTTTCCAGTAGTTTAAAAGCAATTCTTATTTGGAGATAAAAAATGAAC
ATTATCCGTCGTCACCATCA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSDSQEIPYHNQLKNRFRGYFPVIIDVETAGFDAKKDALLELAAITLKMDENGYLHPDQKCHFHIKPFEGANINPESLKF
NGIDIHNPLRGAVSELDAITGLFQMVRRGQKDADCQRSIIVAHNAAFDQSFVMAAAERTGVKRNPFHPFGMFDTASLAGL
MFGQTVLVKACQAAKIPFDGKQAHSALYDTERTAKLFCYMVNHLKDLGGFPHIASELEQEKTTEKETAL

Sequences:

>Translated_229_residues
MSDSQEIPYHNQLKNRFRGYFPVIIDVETAGFDAKKDALLELAAITLKMDENGYLHPDQKCHFHIKPFEGANINPESLKF
NGIDIHNPLRGAVSELDAITGLFQMVRRGQKDADCQRSIIVAHNAAFDQSFVMAAAERTGVKRNPFHPFGMFDTASLAGL
MFGQTVLVKACQAAKIPFDGKQAHSALYDTERTAKLFCYMVNHLKDLGGFPHIASELEQEKTTEKETAL
>Mature_228_residues
SDSQEIPYHNQLKNRFRGYFPVIIDVETAGFDAKKDALLELAAITLKMDENGYLHPDQKCHFHIKPFEGANINPESLKFN
GIDIHNPLRGAVSELDAITGLFQMVRRGQKDADCQRSIIVAHNAAFDQSFVMAAAERTGVKRNPFHPFGMFDTASLAGLM
FGQTVLVKACQAAKIPFDGKQAHSALYDTERTAKLFCYMVNHLKDLGGFPHIASELEQEKTTEKETAL

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=216, Percent_Identity=63.8888888888889, Blast_Score=289, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 25544; Mature: 25413

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSQEIPYHNQLKNRFRGYFPVIIDVETAGFDAKKDALLELAAITLKMDENGYLHPDQK
CCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHEEEEECCCCCCCCCCC
CHFHIKPFEGANINPESLKFNGIDIHNPLRGAVSELDAITGLFQMVRRGQKDADCQRSII
EEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEE
VAHNAAFDQSFVMAAAERTGVKRNPFHPFGMFDTASLAGLMFGQTVLVKACQAAKIPFDG
EEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KQAHSALYDTERTAKLFCYMVNHLKDLGGFPHIASELEQEKTTEKETAL
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDSQEIPYHNQLKNRFRGYFPVIIDVETAGFDAKKDALLELAAITLKMDENGYLHPDQK
CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHEEEEECCCCCCCCCCC
CHFHIKPFEGANINPESLKFNGIDIHNPLRGAVSELDAITGLFQMVRRGQKDADCQRSII
EEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEE
VAHNAAFDQSFVMAAAERTGVKRNPFHPFGMFDTASLAGLMFGQTVLVKACQAAKIPFDG
EEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KQAHSALYDTERTAKLFCYMVNHLKDLGGFPHIASELEQEKTTEKETAL
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA