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The map label for this gene is guaB [C]

Identifier: 16272184

GI number: 16272184

Start: 250524

End: 251015

Strand: Direct

Name: guaB [C]

Synonym: HI0221.1

Alternate gene names: 16272184

Gene position: 250524-251015 (Clockwise)

Preceding gene: 16272183

Following gene: 16272185

Centisome position: 13.69

GC content: 43.99

Gene sequence:

>492_bases
ATGACAAATATCCACTATCACAAAATCCTAATCCTCGACTTTGGTTCACAATACACTCAACTGATTGCGCGTCGTGTGCG
TGAAATCGGTGTGTACTGCGAACTTTGGGCTTGGGATGTAACCGAACAANAAATCCGTGAATTTGCTCCCGAACTTTATC
AAGGTCGTGCATTTAAATCTTACCGTGGTATGGGCTCATTAGGTGCCATGGCGAAAGGCTCATCAGACCGTTATTTCCAA
TCGGATAACGCGGCAGATAAACTCGTACCGGAAGGTATTGAAGGCCGTATTCCATATAAAGGTTACTTAAAAGAAATCAT
CCACCAACAAATGGGTGGCTTACGTTCTTGTATGGGCTTAACGGGCTGTGCAACTATTGATGAACTACGCACCAAAGCGG
AATTTGTTCGCATTAGCGGTGCAGGCATTAAAGAAAGCCACGTTCACGATGTTGCTATTACTAAAGAAGCACCTAACTAT
CGTATGGGCTAA

Upstream 100 bases:

>100_bases
TGGGCTTTAGCCTGCCTTTTGCTGTTTAAAGTGCGGTTGGTTTTTGCAAAGTTTTAAATAATATGAACCGCTTACACTAT
CAATAATAAGAGCATTATAA

Downstream 100 bases:

>100_bases
TCTTTAAGGTGGGCTTTAGCCTGCCTTTTGCTGTTTAAAGTGCGGTTGGTTTTTGCAAAGTTTTAAATAATATGAACCGC
TTACACTATCAATAATAAGA

Product: inosine-5'-monophosphate dehydrogenase-like protein

Products: H+; Nicotinamide adenine dinucleotide - reduced; Xanthosine 5'-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTNIHYHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEQXIREFAPELYQGRAFKSYRGMGSLGAMAKGSSDRYFQ
SDNAADKLVPEGIEGRIPYKGYLKEIIHQQMGGLRSCMGLTGCATIDELRTKAEFVRISGAGIKESHVHDVAITKEAPNY
RMG

Sequences:

>Translated_163_residues
MTNIHYHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEQXIREFAPELYQGRAFKSYRGMGSLGAMAKGSSDRYFQ
SDNAADKLVPEGIEGRIPYKGYLKEIIHQQMGGLRSCMGLTGCATIDELRTKAEFVRISGAGIKESHVHDVAITKEAPNY
RMG
>Mature_162_residues
TNIHYHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEQXIREFAPELYQGRAFKSYRGMGSLGAMAKGSSDRYFQS
DNAADKLVPEGIEGRIPYKGYLKEIIHQQMGGLRSCMGLTGCATIDELRTKAEFVRISGAGIKESHVHDVAITKEAPNYR
MG

Specific function: GMP biosynthesis from IMP; first step. [C]

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IMPDH/GMPR family

Homologues:

Organism=Escherichia coli, GI1788855, Length=113, Percent_Identity=90.2654867256637, Blast_Score=221, Evalue=2e-59,
Organism=Escherichia coli, GI1788854, Length=48, Percent_Identity=83.3333333333333, Blast_Score=94, Evalue=4e-21,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): Y221A_HAEIN (O86223)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_438393.1
- ProteinModelPortal:   O86223
- SMR:   O86223
- GeneID:   951134
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0221.1
- NMPDR:   fig|71421.1.peg.211
- TIGR:   HI_0221.1
- BioCyc:   HINF71421:HI_0221.1-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR001093
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00478 IMPDH

EC number: 1.1.1.205 [C]

Molecular weight: Translated: 18353; Mature: 18222

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIHYHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEQXIREFAPELYQGRAFKS
CCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCHHHH
YRGMGSLGAMAKGSSDRYFQSDNAADKLVPEGIEGRIPYKGYLKEIIHQQMGGLRSCMGL
HCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
TGCATIDELRTKAEFVRISGAGIKESHVHDVAITKEAPNYRMG
CCCHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
TNIHYHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEQXIREFAPELYQGRAFKS
CCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCHHHH
YRGMGSLGAMAKGSSDRYFQSDNAADKLVPEGIEGRIPYKGYLKEIIHQQMGGLRSCMGL
HCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
TGCATIDELRTKAEFVRISGAGIKESHVHDVAITKEAPNYRMG
CCCHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; IMP; Nicotinamide adenine dinucleotide [C]

Specific reaction: H2O + IMP + Nicotinamide adenine dinucleotide --> H+ + Nicotinamide adenine dinucleotide - reduced + Xanthosine 5'-phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800