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The map label for this gene is gdhA

Identifier: 16272153

GI number: 16272153

Start: 203291

End: 204640

Strand: Direct

Name: gdhA

Synonym: HI0189

Alternate gene names: 16272153

Gene position: 203291-204640 (Clockwise)

Preceding gene: 16272152

Following gene: 16272157

Centisome position: 11.11

GC content: 39.56

Gene sequence:

>1350_bases
ATGTCAAAAGTTGCTTCCTTAGACGCATTTTTAACAAAAGTTGCTCAACGCGATGGTTATCAACCTGAATTTTTACAAGC
GGTTCGCGAGGTATTCACATCAATTTGGCCTTTTTTAGAAGCCAATCCTAAATATCGTTCAGAAGCATTATTAGAACGTT
TAGTTGAGCCTGAACGTGCATTTCAATTCCGTGTGGCGTGGACTGACGATAAAGGGCAAGTGCAAGTAAACAGAGCATTT
CGTGTACAATTTAATAGTGCCATAGGCCCATTTAAAGGGGGAATGCGTTTCCATCCATCAGTAAATTTATCTATCTTAAA
ATTCTTAGGTTTTGAGCAAATCTTTAAAAATGCTTTAACAACATTGCCTATGGGCGGGGCAAAAGGCGGTTCAGATTTTG
ATCCTAAAGGCAAATCTGATGCTGAAGTTATGCGTTTTTGCCAAGCATTAATGGCTGAACTTTATCGTCACGTAGGAGCT
GATACAGATGTTCCCGCAGGCGATATAGGCGTCGGTGGGCGCGAAGTTGGCTATTTAGCTGGCTATATGAAAAAATTATC
AAACCAATCAGCCTGTGTTTTCACTGGTCGCGGTCTTTCTTTCGGTGGTAGTTTAATTCGTCCGGAAGCAACGGGATATG
GATTAATTTATTTTGCTCAAGCAATGCTTGCTGAAAAAGGCGATAGTTTTGCAGGTAAAGTAGTTTCAGTTTCTGGTTCT
GGTAATGTAGCACAATATGCTATTGAAAAAGCATTATCTCTTGGTGCAAAAGTAGTAACTTGTTCTGATTCATCAGGTTA
TGTTTATGATCCAAATGGATTTACTACTGAAAAATTAGCCGCACTTTTCGATATTAAAAATACAAAACGTGGGCGTGTGA
AAGATTATGCAGAACAGTTTGGTTTGCAATATTTTGAAGGTAAACGCCCTTGGGAAGTGCAAGTTGATATTGCGCTTCCT
TGTGCAACTCAAAATGAATTAGAACTTTCTGATGCACAACGTTTAATTAAAAATGGTGTGAAATTAGTGGCTGAAGGTGC
GAATATGCCAACAACAATTGAAGCAACAGAAGCATTACTAGCTGCAGATGTATTATTTGGCCCGGGTAAAGCTGCCAACG
CTGGTGGTGTTGCTACTTCTGGTTTAGAAATGGCACAAAGTTCACAACGTTTATATTGGACAGCGGAAGAAGTGGACGCT
CAATTACATCGCATTATGTTAGATATTCACGCAAACTGTAAAAAATACGGCACAATTGAAGGTCAAGAAAACATTAACTA
TGTTGTTGGGGCAAATGTAGCAGGCTTTGTTAAGGTGGCTGATGCAATGTTAGCCCAAGGCGTTTATTAA

Upstream 100 bases:

>100_bases
TGCAAGATTAAATTAGCCATTGCATATTGTCTATGCGCCTCACGAGGCGAATACAATAAAAATACAATACACACAACATC
AACAAAGAGGGAAATTCATT

Downstream 100 bases:

>100_bases
CAAATAAAAAAGTTAAACTAAATAACCGCACTGTAATAAAGTGCGGTTATTTTTTTAGGATGAAATTTGAATATAAGAAA
GCGACATTTTGTCGCCTTAG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSKVASLDAFLTKVAQRDGYQPEFLQAVREVFTSIWPFLEANPKYRSEALLERLVEPERAFQFRVAWTDDKGQVQVNRAF
RVQFNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALTTLPMGGAKGGSDFDPKGKSDAEVMRFCQALMAELYRHVGA
DTDVPAGDIGVGGREVGYLAGYMKKLSNQSACVFTGRGLSFGGSLIRPEATGYGLIYFAQAMLAEKGDSFAGKVVSVSGS
GNVAQYAIEKALSLGAKVVTCSDSSGYVYDPNGFTTEKLAALFDIKNTKRGRVKDYAEQFGLQYFEGKRPWEVQVDIALP
CATQNELELSDAQRLIKNGVKLVAEGANMPTTIEATEALLAADVLFGPGKAANAGGVATSGLEMAQSSQRLYWTAEEVDA
QLHRIMLDIHANCKKYGTIEGQENINYVVGANVAGFVKVADAMLAQGVY

Sequences:

>Translated_449_residues
MSKVASLDAFLTKVAQRDGYQPEFLQAVREVFTSIWPFLEANPKYRSEALLERLVEPERAFQFRVAWTDDKGQVQVNRAF
RVQFNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALTTLPMGGAKGGSDFDPKGKSDAEVMRFCQALMAELYRHVGA
DTDVPAGDIGVGGREVGYLAGYMKKLSNQSACVFTGRGLSFGGSLIRPEATGYGLIYFAQAMLAEKGDSFAGKVVSVSGS
GNVAQYAIEKALSLGAKVVTCSDSSGYVYDPNGFTTEKLAALFDIKNTKRGRVKDYAEQFGLQYFEGKRPWEVQVDIALP
CATQNELELSDAQRLIKNGVKLVAEGANMPTTIEATEALLAADVLFGPGKAANAGGVATSGLEMAQSSQRLYWTAEEVDA
QLHRIMLDIHANCKKYGTIEGQENINYVVGANVAGFVKVADAMLAQGVY
>Mature_448_residues
SKVASLDAFLTKVAQRDGYQPEFLQAVREVFTSIWPFLEANPKYRSEALLERLVEPERAFQFRVAWTDDKGQVQVNRAFR
VQFNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALTTLPMGGAKGGSDFDPKGKSDAEVMRFCQALMAELYRHVGAD
TDVPAGDIGVGGREVGYLAGYMKKLSNQSACVFTGRGLSFGGSLIRPEATGYGLIYFAQAMLAEKGDSFAGKVVSVSGSG
NVAQYAIEKALSLGAKVVTCSDSSGYVYDPNGFTTEKLAALFDIKNTKRGRVKDYAEQFGLQYFEGKRPWEVQVDIALPC
ATQNELELSDAQRLIKNGVKLVAEGANMPTTIEATEALLAADVLFGPGKAANAGGVATSGLEMAQSSQRLYWTAEEVDAQ
LHRIMLDIHANCKKYGTIEGQENINYVVGANVAGFVKVADAMLAQGVY

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family

Homologues:

Organism=Homo sapiens, GI31377775, Length=370, Percent_Identity=29.7297297297297, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI4885281, Length=409, Percent_Identity=28.6063569682152, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI1788059, Length=448, Percent_Identity=74.1071428571429, Blast_Score=684, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=333, Percent_Identity=30.6306306306306, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=53.0973451327434, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319256, Length=453, Percent_Identity=52.317880794702, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI45549226, Length=341, Percent_Identity=30.791788856305, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45553475, Length=341, Percent_Identity=30.791788856305, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24649283, Length=352, Percent_Identity=28.9772727272727, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24649460, Length=294, Percent_Identity=29.9319727891156, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DHE4_HAEIN (P43793)

Other databases:

- EMBL:   L42023
- PIR:   A64053
- RefSeq:   NP_438358.1
- ProteinModelPortal:   P43793
- SMR:   P43793
- GeneID:   951095
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0189
- NMPDR:   fig|71421.1.peg.179
- TIGR:   HI_0189
- HOGENOM:   HBG590661
- OMA:   KGLTYGG
- ProtClustDB:   PRK09414
- BioCyc:   HINF71421:HI_0189-MONOMER
- BRENDA:   1.4.1.4
- GO:   GO:0005488
- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11606:SF2
- PIRSF:   PIRSF000185
- PRINTS:   PR00082
- SMART:   SM00839

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N

EC number: =1.4.1.4

Molecular weight: Translated: 48661; Mature: 48530

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVASLDAFLTKVAQRDGYQPEFLQAVREVFTSIWPFLEANPKYRSEALLERLVEPERA
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHH
FQFRVAWTDDKGQVQVNRAFRVQFNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALT
EEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGAKGGSDFDPKGKSDAEVMRFCQALMAELYRHVGADTDVPAGDIGVGGREVGYLA
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GYMKKLSNQSACVFTGRGLSFGGSLIRPEATGYGLIYFAQAMLAEKGDSFAGKVVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCC
GNVAQYAIEKALSLGAKVVTCSDSSGYVYDPNGFTTEKLAALFDIKNTKRGRVKDYAEQF
CCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHC
GLQYFEGKRPWEVQVDIALPCATQNELELSDAQRLIKNGVKLVAEGANMPTTIEATEALL
CCHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
AADVLFGPGKAANAGGVATSGLEMAQSSQRLYWTAEEVDAQLHRIMLDIHANCKKYGTIE
HHHHHCCCCCCCCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHCCCCC
GQENINYVVGANVAGFVKVADAMLAQGVY
CCCCCCEEECCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKVASLDAFLTKVAQRDGYQPEFLQAVREVFTSIWPFLEANPKYRSEALLERLVEPERA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHH
FQFRVAWTDDKGQVQVNRAFRVQFNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALT
EEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGAKGGSDFDPKGKSDAEVMRFCQALMAELYRHVGADTDVPAGDIGVGGREVGYLA
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GYMKKLSNQSACVFTGRGLSFGGSLIRPEATGYGLIYFAQAMLAEKGDSFAGKVVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCC
GNVAQYAIEKALSLGAKVVTCSDSSGYVYDPNGFTTEKLAALFDIKNTKRGRVKDYAEQF
CCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHC
GLQYFEGKRPWEVQVDIALPCATQNELELSDAQRLIKNGVKLVAEGANMPTTIEATEALL
CCHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
AADVLFGPGKAANAGGVATSGLEMAQSSQRLYWTAEEVDAQLHRIMLDIHANCKKYGTIE
HHHHHCCCCCCCCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHCCCCC
GQENINYVVGANVAGFVKVADAMLAQGVY
CCCCCCEEECCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800