Click here to switch to the map view.

The map label for this gene is mpl

Identifier: 16272091

GI number: 16272091

Start: 135589

End: 136950

Strand: Reverse

Name: mpl

Synonym: HI0121

Alternate gene names: 16272091

Gene position: 136950-135589 (Counterclockwise)

Preceding gene: 16272092

Following gene: 16272090

Centisome position: 7.48

GC content: 39.5

Gene sequence:

>1362_bases
ATGAAACATATTCATATCTTAGGTATTTGTGGCACATTTATGGGCGGTGTGGCGATGATTGCTAAACAAATGGGCTATCA
CGTAACGGGTTCCGATACTAATGTTTACCCTCCAATGAGCACTTTTTTAGAAGAGCAAGGTATCGAGATTATTCCAAATT
ATGATGTTGCACAATTACAACCTGCGCCTGATATGGTAATTGTTGGTAATGCGATGAAACGTGGTAATCCTTGCGTAGAA
TATGTGTTGGAAAATAATTTGAAATACACATCTGGCCCTCAATGGTTACATGATCATTTATTGCGCGATCGTTGGGTATT
GGCGGTTTCAGGTACGCATGGAAAAACAACGACCACAGGTATGCTAACGTGGGTCTTAGAACAAAATGGCTTAAAATCAG
GTTTTTTAATCGGCGGAATTGCGGGTAATTTTGGCATTTCAGCTCGTTTAGGAGATAGCCCTTATTTTATTATTGAAGCT
GATGAATATGATACAGCTTTCTTTGATAAACGTTCGAAATTTGTCCATTACAATCCACGTACACTTATCGTAAACAATAT
TAGTTTTGATCACGCTGATATTTTTGATGATCTCAAAGCGATCCAACGCCAATTTCATCATATGATTCGAACTATCCCAG
CCAGTGGCTTGGTATTAAGTTCGGCGAGTGAACAAAGTGCAAAAGAAACTTTGGCATTGGGATGTTGGTCTCAACAGCAA
TTTTTAGGCAAAGATAACGAGTGGTTTGCTGAACGTATTACAAATGATGCTTCACATTTTGCGGTTTTCCATCATGGAGA
AAAAGTGGCAGAAGTAAAATGGAATGTAGTGGGACAACATAATATGCATAATGCGTTAATGGCAATAGCTGCCGCTCATC
ATACTGGCGTTGCTATTGAAGATGCGTGCAAAGCGTTGGGATCTTTTGTGAATGCTAAACGTCGTTTAGAAGTGAAAGGC
GAAGTTAATAGCATTACGGTTTATGATGATTTTGCGCATCATCCAGAAGCTATTCTTGCTACGCTAACCGCTTTACGAGA
TAAAGTTGGTGGTGGTGTTCGTATTCTTGCAGTGCTTGAACCTCGTTCTAACACAATGAAAATGGGTGTTCATAAAGATG
ACATCGCGCCTGCACTTGGTCGAGCTGATGCCGTGTTTATGCTTCAACCTGAACAACTTTCTTGGGAAGTGGCAGATATT
GCAAACCAATGTGTACAACCTGCTTATTGGAATGCAAATTTAGATCGATTAGTGGATATGATTGTTGCAAAGGCTCAACC
TACGGATCATATTTTAGTGATGTCTAACGGCAGTTTTGGTGGCATTCATCAAAAAATTCTTGATAAGCTAAAACTGAAAT
AA

Upstream 100 bases:

>100_bases
TTATAGATCATATAGTTTGGTATGATGGTCTTTTCTTTTGAAAGTTCATAGAAGTTGTTTTATCATATTTTTCGTTTTCC
TTAATCAACAGAGAAATACA

Downstream 100 bases:

>100_bases
TAAAAGTGCGGTTAATTTAGAACCTGTTTTTAAATTGACTGTACTTTTTATTTCTGACTTTTAGGATATATTTATTTTTA
ATTTGTAATTCATAAAAAGG

Product: UDP-N-acetylmuramoylalanine-D-glutamate ligase-like protein

Products: NA

Alternate protein names: Murein peptide ligase

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQPAPDMVIVGNAMKRGNPCVE
YVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTGMLTWVLEQNGLKSGFLIGGIAGNFGISARLGDSPYFIIEA
DEYDTAFFDKRSKFVHYNPRTLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIEDACKALGSFVNAKRRLEVKG
EVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLEPRSNTMKMGVHKDDIAPALGRADAVFMLQPEQLSWEVADI
ANQCVQPAYWNANLDRLVDMIVAKAQPTDHILVMSNGSFGGIHQKILDKLKLK

Sequences:

>Translated_453_residues
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQPAPDMVIVGNAMKRGNPCVE
YVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTGMLTWVLEQNGLKSGFLIGGIAGNFGISARLGDSPYFIIEA
DEYDTAFFDKRSKFVHYNPRTLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIEDACKALGSFVNAKRRLEVKG
EVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLEPRSNTMKMGVHKDDIAPALGRADAVFMLQPEQLSWEVADI
ANQCVQPAYWNANLDRLVDMIVAKAQPTDHILVMSNGSFGGIHQKILDKLKLK
>Mature_453_residues
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQPAPDMVIVGNAMKRGNPCVE
YVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTGMLTWVLEQNGLKSGFLIGGIAGNFGISARLGDSPYFIIEA
DEYDTAFFDKRSKFVHYNPRTLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIEDACKALGSFVNAKRRLEVKG
EVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLEPRSNTMKMGVHKDDIAPALGRADAVFMLQPEQLSWEVADI
ANQCVQPAYWNANLDRLVDMIVAKAQPTDHILVMSNGSFGGIHQKILDKLKLK

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1790680, Length=450, Percent_Identity=68.2222222222222, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=482, Percent_Identity=28.6307053941909, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MPL_HAEIN (P43948)

Other databases:

- EMBL:   L42023
- PIR:   B64002
- RefSeq:   NP_438293.1
- ProteinModelPortal:   P43948
- GeneID:   951026
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0121
- NMPDR:   fig|71421.1.peg.115
- TIGR:   HI_0121
- HOGENOM:   HBG476594
- OMA:   PYTSGPQ
- ProtClustDB:   CLSK870302
- BioCyc:   HINF71421:HI_0121-MONOMER
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01081

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50075; Mature: 50075

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQ
CCEEEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCEEECCCCHHEEC
PAPDMVIVGNAMKRGNPCVEYVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTG
CCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHH
MLTWVLEQNGLKSGFLIGGIAGNFGISARLGDSPYFIIEADEYDTAFFDKRSKFVHYNPR
EEEEEEECCCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCCCCHHHHCCCCEEEECCC
TLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEEECCCHHH
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIE
HCCCCCHHHHHHHCCCCCEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHH
DACKALGSFVNAKRRLEVKGEVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLE
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEC
PRSNTMKMGVHKDDIAPALGRADAVFMLQPEQLSWEVADIANQCVQPAYWNANLDRLVDM
CCCCCEEECCCHHHCCHHCCCCCEEEEECCHHHCCHHHHHHHHHCCCCCCCCCHHHHHHH
IVAKAQPTDHILVMSNGSFGGIHQKILDKLKLK
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKHIHILGICGTFMGGVAMIAKQMGYHVTGSDTNVYPPMSTFLEEQGIEIIPNYDVAQLQ
CCEEEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCEEECCCCHHEEC
PAPDMVIVGNAMKRGNPCVEYVLENNLKYTSGPQWLHDHLLRDRWVLAVSGTHGKTTTTG
CCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHH
MLTWVLEQNGLKSGFLIGGIAGNFGISARLGDSPYFIIEADEYDTAFFDKRSKFVHYNPR
EEEEEEECCCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCCCCHHHHCCCCEEEECCC
TLIVNNISFDHADIFDDLKAIQRQFHHMIRTIPASGLVLSSASEQSAKETLALGCWSQQQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEEECCCHHH
FLGKDNEWFAERITNDASHFAVFHHGEKVAEVKWNVVGQHNMHNALMAIAAAHHTGVAIE
HCCCCCHHHHHHHCCCCCEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHH
DACKALGSFVNAKRRLEVKGEVNSITVYDDFAHHPEAILATLTALRDKVGGGVRILAVLE
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEC
PRSNTMKMGVHKDDIAPALGRADAVFMLQPEQLSWEVADIANQCVQPAYWNANLDRLVDM
CCCCCEEECCCHHHCCHHCCCCCEEEEECCHHHCCHHHHHHHHHCCCCCCCCCHHHHHHH
IVAKAQPTDHILVMSNGSFGGIHQKILDKLKLK
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800